Amino acid dipepetide frequency for Streptomyces sp. BK161

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.222AlaAla: 20.222 ± 0.134
1.117AlaCys: 1.117 ± 0.023
8.206AlaAsp: 8.206 ± 0.057
8.504AlaGlu: 8.504 ± 0.085
3.493AlaPhe: 3.493 ± 0.034
12.415AlaGly: 12.415 ± 0.078
2.93AlaHis: 2.93 ± 0.03
3.409AlaIle: 3.409 ± 0.037
2.881AlaLys: 2.881 ± 0.046
14.349AlaLeu: 14.349 ± 0.088
2.368AlaMet: 2.368 ± 0.032
2.002AlaAsn: 2.002 ± 0.03
7.032AlaPro: 7.032 ± 0.07
3.846AlaGln: 3.846 ± 0.041
10.099AlaArg: 10.099 ± 0.073
6.046AlaSer: 6.046 ± 0.047
7.129AlaThr: 7.129 ± 0.058
12.095AlaVal: 12.095 ± 0.075
1.912AlaTrp: 1.912 ± 0.025
2.808AlaTyr: 2.808 ± 0.031
0.0AlaXaa: 0.0 ± 0.0
Cys
1.108CysAla: 1.108 ± 0.021
0.104CysCys: 0.104 ± 0.006
0.465CysAsp: 0.465 ± 0.013
0.413CysGlu: 0.413 ± 0.013
0.223CysPhe: 0.223 ± 0.008
0.969CysGly: 0.969 ± 0.021
0.203CysHis: 0.203 ± 0.008
0.146CysIle: 0.146 ± 0.008
0.128CysLys: 0.128 ± 0.007
0.764CysLeu: 0.764 ± 0.016
0.114CysMet: 0.114 ± 0.006
0.145CysAsn: 0.145 ± 0.007
0.496CysPro: 0.496 ± 0.013
0.178CysGln: 0.178 ± 0.009
0.614CysArg: 0.614 ± 0.017
0.442CysSer: 0.442 ± 0.015
0.531CysThr: 0.531 ± 0.014
0.685CysVal: 0.685 ± 0.013
0.138CysTrp: 0.138 ± 0.007
0.153CysTyr: 0.153 ± 0.006
0.0CysXaa: 0.0 ± 0.0
Asp
7.562AspAla: 7.562 ± 0.055
0.433AspCys: 0.433 ± 0.013
3.727AspAsp: 3.727 ± 0.034
3.844AspGlu: 3.844 ± 0.041
1.668AspPhe: 1.668 ± 0.026
6.379AspGly: 6.379 ± 0.055
1.451AspHis: 1.451 ± 0.022
1.929AspIle: 1.929 ± 0.028
1.245AspLys: 1.245 ± 0.026
6.199AspLeu: 6.199 ± 0.052
0.827AspMet: 0.827 ± 0.016
1.052AspAsn: 1.052 ± 0.02
4.496AspPro: 4.496 ± 0.045
1.578AspGln: 1.578 ± 0.025
4.857AspArg: 4.857 ± 0.048
2.673AspSer: 2.673 ± 0.029
3.47AspThr: 3.47 ± 0.033
4.815AspVal: 4.815 ± 0.042
1.055AspTrp: 1.055 ± 0.018
1.17AspTyr: 1.17 ± 0.024
0.0AspXaa: 0.0 ± 0.0
Glu
7.312GluAla: 7.312 ± 0.075
0.371GluCys: 0.371 ± 0.011
2.843GluAsp: 2.843 ± 0.032
3.567GluGlu: 3.567 ± 0.044
1.466GluPhe: 1.466 ± 0.026
4.3GluGly: 4.3 ± 0.04
1.535GluHis: 1.535 ± 0.024
2.171GluIle: 2.171 ± 0.026
1.434GluLys: 1.434 ± 0.023
6.67GluLeu: 6.67 ± 0.064
0.857GluMet: 0.857 ± 0.017
1.035GluAsn: 1.035 ± 0.02
3.366GluPro: 3.366 ± 0.04
2.277GluGln: 2.277 ± 0.027
5.399GluArg: 5.399 ± 0.052
2.59GluSer: 2.59 ± 0.031
2.912GluThr: 2.912 ± 0.036
4.421GluVal: 4.421 ± 0.043
0.811GluTrp: 0.811 ± 0.016
1.126GluTyr: 1.126 ± 0.02
0.0GluXaa: 0.0 ± 0.0
Phe
3.54PheAla: 3.54 ± 0.036
0.262PheCys: 0.262 ± 0.01
1.95PheAsp: 1.95 ± 0.028
1.444PheGlu: 1.444 ± 0.023
0.886PhePhe: 0.886 ± 0.02
2.985PheGly: 2.985 ± 0.033
0.634PheHis: 0.634 ± 0.014
0.709PheIle: 0.709 ± 0.017
0.529PheLys: 0.529 ± 0.014
2.585PheLeu: 2.585 ± 0.037
0.394PheMet: 0.394 ± 0.011
0.591PheAsn: 0.591 ± 0.013
1.383PhePro: 1.383 ± 0.022
0.744PheGln: 0.744 ± 0.015
1.843PheArg: 1.843 ± 0.024
1.492PheSer: 1.492 ± 0.022
2.051PheThr: 2.051 ± 0.029
2.264PheVal: 2.264 ± 0.027
0.436PheTrp: 0.436 ± 0.011
0.592PheTyr: 0.592 ± 0.013
0.0PheXaa: 0.0 ± 0.0
Gly
10.86GlyAla: 10.86 ± 0.077
0.832GlyCys: 0.832 ± 0.018
5.138GlyAsp: 5.138 ± 0.049
4.999GlyGlu: 4.999 ± 0.046
2.867GlyPhe: 2.867 ± 0.036
8.699GlyGly: 8.699 ± 0.084
2.323GlyHis: 2.323 ± 0.029
3.353GlyIle: 3.353 ± 0.035
2.447GlyLys: 2.447 ± 0.038
9.34GlyLeu: 9.34 ± 0.068
1.914GlyMet: 1.914 ± 0.029
1.833GlyAsn: 1.833 ± 0.033
5.086GlyPro: 5.086 ± 0.051
2.662GlyGln: 2.662 ± 0.033
7.662GlyArg: 7.662 ± 0.057
5.361GlySer: 5.361 ± 0.052
6.502GlyThr: 6.502 ± 0.065
7.599GlyVal: 7.599 ± 0.058
1.707GlyTrp: 1.707 ± 0.029
2.311GlyTyr: 2.311 ± 0.032
0.0GlyXaa: 0.0 ± 0.0
His
2.696HisAla: 2.696 ± 0.033
0.212HisCys: 0.212 ± 0.008
1.373HisAsp: 1.373 ± 0.023
1.244HisGlu: 1.244 ± 0.022
0.675HisPhe: 0.675 ± 0.014
2.433HisGly: 2.433 ± 0.032
0.721HisHis: 0.721 ± 0.019
0.676HisIle: 0.676 ± 0.014
0.374HisLys: 0.374 ± 0.013
2.477HisLeu: 2.477 ± 0.032
0.33HisMet: 0.33 ± 0.01
0.397HisAsn: 0.397 ± 0.01
1.895HisPro: 1.895 ± 0.028
0.642HisGln: 0.642 ± 0.015
2.098HisArg: 2.098 ± 0.033
1.043HisSer: 1.043 ± 0.02
1.453HisThr: 1.453 ± 0.023
1.737HisVal: 1.737 ± 0.027
0.383HisTrp: 0.383 ± 0.011
0.511HisTyr: 0.511 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
4.584IleAla: 4.584 ± 0.042
0.269IleCys: 0.269 ± 0.009
2.199IleAsp: 2.199 ± 0.031
1.889IleGlu: 1.889 ± 0.023
0.671IlePhe: 0.671 ± 0.016
3.367IleGly: 3.367 ± 0.034
0.614IleHis: 0.614 ± 0.015
0.835IleIle: 0.835 ± 0.016
0.759IleLys: 0.759 ± 0.018
2.384IleLeu: 2.384 ± 0.029
0.444IleMet: 0.444 ± 0.014
0.725IleAsn: 0.725 ± 0.016
1.773IlePro: 1.773 ± 0.027
0.734IleGln: 0.734 ± 0.016
2.195IleArg: 2.195 ± 0.029
1.67IleSer: 1.67 ± 0.024
2.254IleThr: 2.254 ± 0.03
2.69IleVal: 2.69 ± 0.033
0.374IleTrp: 0.374 ± 0.012
0.522IleTyr: 0.522 ± 0.015
0.0IleXaa: 0.0 ± 0.0
Lys
3.027LysAla: 3.027 ± 0.044
0.114LysCys: 0.114 ± 0.007
1.39LysAsp: 1.39 ± 0.029
1.204LysGlu: 1.204 ± 0.019
0.458LysPhe: 0.458 ± 0.015
1.876LysGly: 1.876 ± 0.031
0.433LysHis: 0.433 ± 0.011
0.86LysIle: 0.86 ± 0.02
0.862LysLys: 0.862 ± 0.025
2.052LysLeu: 2.052 ± 0.03
0.371LysMet: 0.371 ± 0.013
0.585LysAsn: 0.585 ± 0.018
1.398LysPro: 1.398 ± 0.029
0.744LysGln: 0.744 ± 0.017
1.413LysArg: 1.413 ± 0.027
1.184LysSer: 1.184 ± 0.022
1.346LysThr: 1.346 ± 0.028
1.968LysVal: 1.968 ± 0.031
0.3LysTrp: 0.3 ± 0.009
0.49LysTyr: 0.49 ± 0.014
0.0LysXaa: 0.0 ± 0.0
Leu
14.71LeuAla: 14.71 ± 0.081
0.849LeuCys: 0.849 ± 0.018
6.65LeuAsp: 6.65 ± 0.053
4.677LeuGlu: 4.677 ± 0.046
2.609LeuPhe: 2.609 ± 0.033
9.119LeuGly: 9.119 ± 0.06
2.326LeuHis: 2.326 ± 0.032
3.178LeuIle: 3.178 ± 0.039
2.117LeuLys: 2.117 ± 0.032
11.169LeuLeu: 11.169 ± 0.081
1.623LeuMet: 1.623 ± 0.025
1.721LeuAsn: 1.721 ± 0.026
6.461LeuPro: 6.461 ± 0.056
2.212LeuGln: 2.212 ± 0.027
8.66LeuArg: 8.66 ± 0.074
5.419LeuSer: 5.419 ± 0.039
7.145LeuThr: 7.145 ± 0.05
8.777LeuVal: 8.777 ± 0.074
1.413LeuTrp: 1.413 ± 0.025
1.93LeuTyr: 1.93 ± 0.028
0.0LeuXaa: 0.0 ± 0.0
Met
2.131MetAla: 2.131 ± 0.028
0.135MetCys: 0.135 ± 0.007
0.86MetAsp: 0.86 ± 0.016
0.718MetGlu: 0.718 ± 0.015
0.439MetPhe: 0.439 ± 0.014
1.29MetGly: 1.29 ± 0.024
0.352MetHis: 0.352 ± 0.012
0.62MetIle: 0.62 ± 0.015
0.427MetLys: 0.427 ± 0.01
1.611MetLeu: 1.611 ± 0.026
0.286MetMet: 0.286 ± 0.01
0.44MetAsn: 0.44 ± 0.011
1.094MetPro: 1.094 ± 0.019
0.433MetGln: 0.433 ± 0.01
1.405MetArg: 1.405 ± 0.02
1.315MetSer: 1.315 ± 0.021
1.578MetThr: 1.578 ± 0.022
1.234MetVal: 1.234 ± 0.022
0.223MetTrp: 0.223 ± 0.009
0.318MetTyr: 0.318 ± 0.01
0.0MetXaa: 0.0 ± 0.0
Asn
2.292AsnAla: 2.292 ± 0.028
0.166AsnCys: 0.166 ± 0.007
1.023AsnAsp: 1.023 ± 0.021
0.83AsnGlu: 0.83 ± 0.017
0.497AsnPhe: 0.497 ± 0.012
2.037AsnGly: 2.037 ± 0.036
0.421AsnHis: 0.421 ± 0.012
0.658AsnIle: 0.658 ± 0.017
0.45AsnLys: 0.45 ± 0.014
1.698AsnLeu: 1.698 ± 0.024
0.303AsnMet: 0.303 ± 0.01
0.492AsnAsn: 0.492 ± 0.016
1.412AsnPro: 1.412 ± 0.025
0.572AsnGln: 0.572 ± 0.016
1.304AsnArg: 1.304 ± 0.02
1.019AsnSer: 1.019 ± 0.02
1.19AsnThr: 1.19 ± 0.026
1.459AsnVal: 1.459 ± 0.024
0.324AsnTrp: 0.324 ± 0.011
0.447AsnTyr: 0.447 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.334ProAla: 8.334 ± 0.067
0.363ProCys: 0.363 ± 0.011
4.548ProAsp: 4.548 ± 0.042
4.364ProGlu: 4.364 ± 0.047
1.526ProPhe: 1.526 ± 0.023
6.498ProGly: 6.498 ± 0.057
1.457ProHis: 1.457 ± 0.024
1.253ProIle: 1.253 ± 0.019
1.212ProLys: 1.212 ± 0.023
5.414ProLeu: 5.414 ± 0.043
0.953ProMet: 0.953 ± 0.016
0.978ProAsn: 0.978 ± 0.02
3.633ProPro: 3.633 ± 0.062
1.779ProGln: 1.779 ± 0.033
3.991ProArg: 3.991 ± 0.049
3.362ProSer: 3.362 ± 0.039
3.434ProThr: 3.434 ± 0.035
5.527ProVal: 5.527 ± 0.054
0.963ProTrp: 0.963 ± 0.017
1.478ProTyr: 1.478 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
3.796GlnAla: 3.796 ± 0.04
0.183GlnCys: 0.183 ± 0.009
1.479GlnAsp: 1.479 ± 0.024
1.457GlnGlu: 1.457 ± 0.025
0.702GlnPhe: 0.702 ± 0.015
2.384GlnGly: 2.384 ± 0.032
0.672GlnHis: 0.672 ± 0.014
1.084GlnIle: 1.084 ± 0.02
0.63GlnLys: 0.63 ± 0.014
3.046GlnLeu: 3.046 ± 0.032
0.488GlnMet: 0.488 ± 0.012
0.522GlnAsn: 0.522 ± 0.014
1.73GlnPro: 1.73 ± 0.031
1.302GlnGln: 1.302 ± 0.028
2.419GlnArg: 2.419 ± 0.027
1.313GlnSer: 1.313 ± 0.018
1.406GlnThr: 1.406 ± 0.024
2.388GlnVal: 2.388 ± 0.033
0.516GlnTrp: 0.516 ± 0.014
0.659GlnTyr: 0.659 ± 0.016
0.0GlnXaa: 0.0 ± 0.0
Arg
9.811ArgAla: 9.811 ± 0.082
0.613ArgCys: 0.613 ± 0.016
4.273ArgAsp: 4.273 ± 0.043
4.735ArgGlu: 4.735 ± 0.051
2.341ArgPhe: 2.341 ± 0.034
5.789ArgGly: 5.789 ± 0.052
2.168ArgHis: 2.168 ± 0.032
3.118ArgIle: 3.118 ± 0.033
1.621ArgLys: 1.621 ± 0.031
8.813ArgLeu: 8.813 ± 0.07
1.675ArgMet: 1.675 ± 0.024
1.346ArgAsn: 1.346 ± 0.022
5.01ArgPro: 5.01 ± 0.051
2.347ArgGln: 2.347 ± 0.028
7.657ArgArg: 7.657 ± 0.073
4.003ArgSer: 4.003 ± 0.035
5.4ArgThr: 5.4 ± 0.05
5.917ArgVal: 5.917 ± 0.046
1.391ArgTrp: 1.391 ± 0.024
1.774ArgTyr: 1.774 ± 0.024
0.0ArgXaa: 0.0 ± 0.0
Ser
6.748SerAla: 6.748 ± 0.05
0.423SerCys: 0.423 ± 0.012
2.852SerAsp: 2.852 ± 0.034
2.46SerGlu: 2.46 ± 0.032
1.524SerPhe: 1.524 ± 0.023
6.075SerGly: 6.075 ± 0.054
1.043SerHis: 1.043 ± 0.021
1.421SerIle: 1.421 ± 0.024
1.066SerLys: 1.066 ± 0.023
4.896SerLeu: 4.896 ± 0.048
1.087SerMet: 1.087 ± 0.02
0.95SerAsn: 0.95 ± 0.017
3.295SerPro: 3.295 ± 0.039
1.291SerGln: 1.291 ± 0.022
3.72SerArg: 3.72 ± 0.036
3.028SerSer: 3.028 ± 0.042
3.273SerThr: 3.273 ± 0.038
4.402SerVal: 4.402 ± 0.04
0.929SerTrp: 0.929 ± 0.017
1.284SerTyr: 1.284 ± 0.023
0.0SerXaa: 0.0 ± 0.0
Thr
9.029ThrAla: 9.029 ± 0.069
0.466ThrCys: 0.466 ± 0.013
3.853ThrAsp: 3.853 ± 0.042
3.326ThrGlu: 3.326 ± 0.037
1.681ThrPhe: 1.681 ± 0.026
6.833ThrGly: 6.833 ± 0.054
1.264ThrHis: 1.264 ± 0.022
1.687ThrIle: 1.687 ± 0.028
1.284ThrLys: 1.284 ± 0.028
5.904ThrLeu: 5.904 ± 0.045
0.93ThrMet: 0.93 ± 0.017
1.138ThrAsn: 1.138 ± 0.023
4.34ThrPro: 4.34 ± 0.043
1.438ThrGln: 1.438 ± 0.027
4.0ThrArg: 4.0 ± 0.04
3.427ThrSer: 3.427 ± 0.043
4.142ThrThr: 4.142 ± 0.055
6.258ThrVal: 6.258 ± 0.049
0.973ThrTrp: 0.973 ± 0.018
1.468ThrTyr: 1.468 ± 0.023
0.0ThrXaa: 0.0 ± 0.0
Val
10.566ValAla: 10.566 ± 0.074
0.773ValCys: 0.773 ± 0.017
5.092ValAsp: 5.092 ± 0.041
4.725ValGlu: 4.725 ± 0.046
2.417ValPhe: 2.417 ± 0.033
6.552ValGly: 6.552 ± 0.05
1.998ValHis: 1.998 ± 0.028
2.774ValIle: 2.774 ± 0.035
1.77ValLys: 1.77 ± 0.029
9.483ValLeu: 9.483 ± 0.065
1.384ValMet: 1.384 ± 0.022
1.712ValAsn: 1.712 ± 0.029
5.3ValPro: 5.3 ± 0.053
2.118ValGln: 2.118 ± 0.028
7.281ValArg: 7.281 ± 0.058
4.405ValSer: 4.405 ± 0.042
5.81ValThr: 5.81 ± 0.05
8.079ValVal: 8.079 ± 0.067
1.218ValTrp: 1.218 ± 0.02
1.649ValTyr: 1.649 ± 0.025
0.0ValXaa: 0.0 ± 0.0
Trp
1.715TrpAla: 1.715 ± 0.026
0.154TrpCys: 0.154 ± 0.007
0.892TrpAsp: 0.892 ± 0.018
0.771TrpGlu: 0.771 ± 0.015
0.52TrpPhe: 0.52 ± 0.014
1.134TrpGly: 1.134 ± 0.018
0.398TrpHis: 0.398 ± 0.012
0.563TrpIle: 0.563 ± 0.013
0.398TrpLys: 0.398 ± 0.013
1.805TrpLeu: 1.805 ± 0.027
0.289TrpMet: 0.289 ± 0.01
0.463TrpAsn: 0.463 ± 0.014
0.805TrpPro: 0.805 ± 0.017
0.646TrpGln: 0.646 ± 0.015
1.375TrpArg: 1.375 ± 0.025
0.994TrpSer: 0.994 ± 0.021
1.133TrpThr: 1.133 ± 0.018
1.022TrpVal: 1.022 ± 0.018
0.341TrpTrp: 0.341 ± 0.013
0.389TrpTyr: 0.389 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.821TyrAla: 2.821 ± 0.03
0.179TyrCys: 0.179 ± 0.009
1.616TyrAsp: 1.616 ± 0.031
1.294TyrGlu: 1.294 ± 0.019
0.652TyrPhe: 0.652 ± 0.016
2.362TyrGly: 2.362 ± 0.033
0.425TyrHis: 0.425 ± 0.012
0.505TyrIle: 0.505 ± 0.013
0.441TyrLys: 0.441 ± 0.014
2.105TyrLeu: 2.105 ± 0.028
0.272TyrMet: 0.272 ± 0.01
0.467TyrAsn: 0.467 ± 0.015
1.062TyrPro: 1.062 ± 0.018
0.634TyrGln: 0.634 ± 0.015
1.813TyrArg: 1.813 ± 0.026
0.982TyrSer: 0.982 ± 0.018
1.279TyrThr: 1.279 ± 0.023
1.77TyrVal: 1.77 ± 0.025
0.4TyrTrp: 0.4 ± 0.012
0.506TyrTyr: 0.506 ± 0.013
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 9221 proteins (3064903 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski