Beutenbergia cavernae (strain ATCC BAA-8 / DSM 12333 / NBRC 16432)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Beutenbergiaceae; Beutenbergia; Beutenbergia cavernae

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4195 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5BY60|C5BY60_BEUC1 Major facilitator superfamily MFS_1 OS=Beutenbergia cavernae (strain ATCC BAA-8 / DSM 12333 / NBRC 16432) OX=471853 GN=Bcav_2715 PE=4 SV=1
MM1 pKa = 7.01SRR3 pKa = 11.84AWRR6 pKa = 11.84EE7 pKa = 3.66GPYY10 pKa = 10.33ADD12 pKa = 3.22PVSRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84AARR21 pKa = 11.84GVAAVLAVAGLAAACAGPAPMLQPVDD47 pKa = 4.06AGAPTDD53 pKa = 4.07PGQQGSATPQPSDD66 pKa = 3.28DD67 pKa = 4.29ALAEE71 pKa = 4.61FYY73 pKa = 10.08TQVPSWYY80 pKa = 8.58ACGGGFEE87 pKa = 4.45CADD90 pKa = 3.36VEE92 pKa = 5.1VPLDD96 pKa = 3.49YY97 pKa = 10.83DD98 pKa = 3.62APDD101 pKa = 3.77GEE103 pKa = 4.43RR104 pKa = 11.84VQLAVKK110 pKa = 10.22RR111 pKa = 11.84LPADD115 pKa = 3.8DD116 pKa = 3.78PAARR120 pKa = 11.84QGSLLVNPGGPGASGLDD137 pKa = 3.9LVDD140 pKa = 3.71SAADD144 pKa = 3.55LFSNRR149 pKa = 11.84VLDD152 pKa = 4.52AYY154 pKa = 10.69DD155 pKa = 3.35VVGFDD160 pKa = 3.64PRR162 pKa = 11.84GVGSSTKK169 pKa = 9.7IVCLGPDD176 pKa = 3.53EE177 pKa = 4.9EE178 pKa = 5.27DD179 pKa = 3.86PSGGDD184 pKa = 3.35YY185 pKa = 11.14DD186 pKa = 5.42LSDD189 pKa = 3.91DD190 pKa = 5.04AEE192 pKa = 4.36VQRR195 pKa = 11.84LVDD198 pKa = 4.89DD199 pKa = 4.93LDD201 pKa = 4.77ALGEE205 pKa = 4.07MCRR208 pKa = 11.84DD209 pKa = 3.41HH210 pKa = 8.93SGDD213 pKa = 4.11LLDD216 pKa = 5.56HH217 pKa = 6.9VDD219 pKa = 3.74TVHH222 pKa = 7.72AARR225 pKa = 11.84DD226 pKa = 3.76LDD228 pKa = 3.72VLRR231 pKa = 11.84AVLGDD236 pKa = 3.48EE237 pKa = 3.9RR238 pKa = 11.84LTYY241 pKa = 10.81LGFSYY246 pKa = 9.06GTILGATFAEE256 pKa = 4.62LFPDD260 pKa = 3.32RR261 pKa = 11.84VGRR264 pKa = 11.84LVLDD268 pKa = 4.13GAIDD272 pKa = 3.75PSIDD276 pKa = 3.66YY277 pKa = 8.45TQMTADD283 pKa = 3.2QVDD286 pKa = 3.88GFEE289 pKa = 4.24VAFRR293 pKa = 11.84SYY295 pKa = 10.91LANCLEE301 pKa = 4.5GDD303 pKa = 3.61ACPFSGTEE311 pKa = 3.72DD312 pKa = 3.35EE313 pKa = 4.97AYY315 pKa = 10.63DD316 pKa = 3.54RR317 pKa = 11.84AVAFLEE323 pKa = 4.18EE324 pKa = 4.91LDD326 pKa = 4.72AEE328 pKa = 4.39PLPSEE333 pKa = 4.71GEE335 pKa = 4.15DD336 pKa = 3.93GEE338 pKa = 4.5LTSDD342 pKa = 3.46EE343 pKa = 4.76AYY345 pKa = 10.52GAIQSSMYY353 pKa = 9.85VAWAWEE359 pKa = 3.81ALSEE363 pKa = 4.33GFTQAFEE370 pKa = 4.53DD371 pKa = 4.21ADD373 pKa = 4.14GSALDD378 pKa = 4.52VIAHH382 pKa = 6.42EE383 pKa = 5.18NDD385 pKa = 4.03DD386 pKa = 4.62PDD388 pKa = 4.47PNADD392 pKa = 3.37FAFWGIDD399 pKa = 3.29CSDD402 pKa = 3.72YY403 pKa = 11.04PITSSVDD410 pKa = 3.5EE411 pKa = 4.71ILAQADD417 pKa = 3.85EE418 pKa = 4.73LEE420 pKa = 4.34EE421 pKa = 4.64ASALFGAAMGTGEE434 pKa = 4.5LVCRR438 pKa = 11.84QWPYY442 pKa = 10.08QSTAVRR448 pKa = 11.84EE449 pKa = 4.32PIEE452 pKa = 4.24AAGAAPILVVGTTRR466 pKa = 11.84DD467 pKa = 3.1PATPYY472 pKa = 10.25RR473 pKa = 11.84WAEE476 pKa = 3.74ALAGQLEE483 pKa = 4.44SGRR486 pKa = 11.84LLTWDD491 pKa = 3.76GDD493 pKa = 3.57GHH495 pKa = 5.17TAYY498 pKa = 10.44ASGSPCIDD506 pKa = 3.56EE507 pKa = 5.37AVQAYY512 pKa = 10.24LLDD515 pKa = 3.84GTLPDD520 pKa = 5.51DD521 pKa = 4.14GTVCC525 pKa = 4.7

Molecular weight: 55.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5C197|C5C197_BEUC1 Thiamine_BP domain-containing protein OS=Beutenbergia cavernae (strain ATCC BAA-8 / DSM 12333 / NBRC 16432) OX=471853 GN=Bcav_3264 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4195

0

4195

1434007

32

4672

341.8

36.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.644 ± 0.058

0.52 ± 0.008

6.634 ± 0.036

5.488 ± 0.036

2.761 ± 0.024

9.442 ± 0.037

2.031 ± 0.021

3.216 ± 0.025

1.088 ± 0.02

10.244 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.597 ± 0.014

1.507 ± 0.017

5.999 ± 0.031

2.403 ± 0.02

7.836 ± 0.047

5.057 ± 0.024

6.131 ± 0.057

9.814 ± 0.037

1.655 ± 0.019

1.932 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski