Hosta virus X

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus

Average proteome isoelectric point is 7.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6Y594|Q6Y594_9VIRU Movement protein TGBp3 OS=Hosta virus X OX=214439 GN=TGB3 PE=3 SV=1
MM1 pKa = 7.55ASDD4 pKa = 4.59APTPPAAPSPVTFTAPTQEE23 pKa = 4.07QLTSLALPIISTRR36 pKa = 11.84LPSPDD41 pKa = 3.38VLNQISVKK49 pKa = 8.05WQEE52 pKa = 4.2LGVPTASISSTAIALCMACYY72 pKa = 10.12HH73 pKa = 6.44SGSSGSTLIPGLAPGTTVNYY93 pKa = 8.55TSLAAAVKK101 pKa = 10.27SLATLRR107 pKa = 11.84EE108 pKa = 3.94FARR111 pKa = 11.84YY112 pKa = 7.38FAPIIWNYY120 pKa = 10.45AIEE123 pKa = 4.58HH124 pKa = 6.88KK125 pKa = 10.53IPPANWAAMGYY136 pKa = 9.85KK137 pKa = 10.38EE138 pKa = 4.08NTKK141 pKa = 10.42YY142 pKa = 10.87AAFDD146 pKa = 3.61TFDD149 pKa = 4.85SILNPAALQPTGGLIRR165 pKa = 11.84QPTEE169 pKa = 3.8EE170 pKa = 4.69EE171 pKa = 4.17LLAHH175 pKa = 5.84QANSALHH182 pKa = 6.52IFDD185 pKa = 4.2SLRR188 pKa = 11.84NDD190 pKa = 3.75FASTDD195 pKa = 3.26GRR197 pKa = 11.84VTRR200 pKa = 11.84GHH202 pKa = 5.86ITSNVNSLNYY212 pKa = 10.27LPAPEE217 pKa = 4.91GSSS220 pKa = 3.17

Molecular weight: 23.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5GR28|Q5GR28_9VIRU ORF1 protein OS=Hosta virus X OX=214439 GN=Xgp1 PE=4 SV=1
MM1 pKa = 7.57SSPHH5 pKa = 7.02RR6 pKa = 11.84LTPPPNYY13 pKa = 9.78TPVLLAVVIGVGLAVVTNQLTRR35 pKa = 11.84STLPHH40 pKa = 6.64VGDD43 pKa = 4.96NIHH46 pKa = 6.38SLPHH50 pKa = 5.72GGNYY54 pKa = 9.71KK55 pKa = 10.74DD56 pKa = 3.58GTKK59 pKa = 10.4SVIYY63 pKa = 9.98RR64 pKa = 11.84GPAPFQRR71 pKa = 11.84SHH73 pKa = 5.15STAPPFNAVLLLTFAIWFLSCRR95 pKa = 11.84TRR97 pKa = 11.84RR98 pKa = 11.84AAIGIHH104 pKa = 5.2VCHH107 pKa = 6.45TCSQTRR113 pKa = 11.84EE114 pKa = 4.03QQQ116 pKa = 2.99

Molecular weight: 12.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2147

74

1507

429.4

48.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.058 ± 1.758

1.49 ± 0.641

4.332 ± 0.808

5.589 ± 1.275

4.658 ± 0.581

4.984 ± 0.673

3.354 ± 0.571

5.217 ± 0.385

6.195 ± 2.178

9.688 ± 1.108

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.677 ± 0.415

4.285 ± 0.526

7.266 ± 0.929

4.565 ± 1.079

5.822 ± 0.768

6.148 ± 1.558

7.965 ± 0.574

4.471 ± 0.898

1.304 ± 0.32

2.934 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski