Botrytis cinerea fusarivirus 1-S1
Average proteome isoelectric point is 7.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U9NJ82|A0A2U9NJ82_9VIRU Uncharacterized protein OS=Botrytis cinerea fusarivirus 1-S1 OX=2219107 PE=4 SV=1
MM1 pKa = 7.43 TGHH4 pKa = 7.64 ASTSADD10 pKa = 2.97 NSLAMVALYY19 pKa = 10.92 ALAWRR24 pKa = 11.84 KK25 pKa = 7.96 LTGTGSQEE33 pKa = 3.52 FLKK36 pKa = 11.14 YY37 pKa = 9.33 NTLSVYY43 pKa = 10.86 GDD45 pKa = 3.59 DD46 pKa = 5.19 HH47 pKa = 7.68 ILSIARR53 pKa = 11.84 NAPVQWTFPNVQSYY67 pKa = 9.62 LASCNITLRR76 pKa = 11.84 EE77 pKa = 3.85 EE78 pKa = 4.23 VQTGGKK84 pKa = 7.45 GTKK87 pKa = 9.94 LEE89 pKa = 4.66 SIPFLSKK96 pKa = 10.01 MARR99 pKa = 11.84 KK100 pKa = 9.07 PSAEE104 pKa = 3.93 EE105 pKa = 3.36 AHH107 pKa = 6.58 LFEE110 pKa = 4.81 SLGIEE115 pKa = 4.23 VPRR118 pKa = 11.84 WTISHH123 pKa = 6.93 DD124 pKa = 3.56 RR125 pKa = 11.84 EE126 pKa = 4.06 KK127 pKa = 11.27 LLGKK131 pKa = 8.17 MTAQLPNQNPLARR144 pKa = 11.84 AKK146 pKa = 10.52 RR147 pKa = 11.84 MQSYY151 pKa = 10.56 LYY153 pKa = 10.46 LCAHH157 pKa = 6.18 NPEE160 pKa = 4.64 AYY162 pKa = 8.26 EE163 pKa = 4.17 ALTVALNTLFGKK175 pKa = 10.09 FPKK178 pKa = 10.09 VEE180 pKa = 4.16 KK181 pKa = 10.43 EE182 pKa = 3.8 LGKK185 pKa = 9.01 YY186 pKa = 6.86 TPSYY190 pKa = 11.19 ARR192 pKa = 11.84 VLKK195 pKa = 10.29 VWYY198 pKa = 9.69 DD199 pKa = 3.47 PEE201 pKa = 4.29 SAPKK205 pKa = 9.45 IDD207 pKa = 4.04 EE208 pKa = 4.2 DD209 pKa = 4.74 HH210 pKa = 7.13 LVGDD214 pKa = 4.72 DD215 pKa = 4.98 DD216 pKa = 4.95 GVSAYY221 pKa = 10.45 GGLTAMDD228 pKa = 3.53 QFLNATAALPDD239 pKa = 3.86 YY240 pKa = 9.94 FNPIMKK246 pKa = 10.44 NNGWVKK252 pKa = 10.55 SIQQLAGTSLFWPNEE267 pKa = 3.86 LIQRR271 pKa = 11.84 TNSHH275 pKa = 5.89 VSDD278 pKa = 3.97 AQVSSSLRR286 pKa = 11.84 KK287 pKa = 8.62 SCYY290 pKa = 9.83 SFLEE294 pKa = 3.94 HH295 pKa = 7.14 RR296 pKa = 11.84 GTVPSSGSTLLTLLLRR312 pKa = 11.84 HH313 pKa = 5.97 WLFILMTPRR322 pKa = 11.84 SSTDD326 pKa = 3.38 AISSVTKK333 pKa = 10.69 VADD336 pKa = 3.98 GVSASAARR344 pKa = 11.84 WNFVLLAKK352 pKa = 9.49 DD353 pKa = 3.59 TYY355 pKa = 10.4 KK356 pKa = 11.0 LEE358 pKa = 4.33 SASLHH363 pKa = 5.67 LKK365 pKa = 7.81 EE366 pKa = 5.52 TLFLWILGFLEE377 pKa = 4.83 VPFEE381 pKa = 4.32 LPSVFYY387 pKa = 10.44 SLRR390 pKa = 11.84 IPP392 pKa = 3.8
Molecular weight: 43.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.831
IPC2_protein 7.029
IPC_protein 7.22
Toseland 7.22
ProMoST 7.761
Dawson 7.819
Bjellqvist 7.732
Wikipedia 7.775
Rodwell 7.805
Grimsley 7.322
Solomon 7.951
Lehninger 7.966
Nozaki 7.951
DTASelect 7.922
Thurlkill 7.966
EMBOSS 8.083
Sillero 8.156
Patrickios 4.291
IPC_peptide 7.951
IPC2_peptide 7.205
IPC2.peptide.svr19 7.355
Protein with the highest isoelectric point:
>tr|A0A2U9NJ82|A0A2U9NJ82_9VIRU Uncharacterized protein OS=Botrytis cinerea fusarivirus 1-S1 OX=2219107 PE=4 SV=1
MM1 pKa = 7.35 WSIIFSVYY9 pKa = 9.0 WPEE12 pKa = 4.56 AIQLIAATFWMWAAFIIVFPAMLILHH38 pKa = 6.73 GLADD42 pKa = 3.64 VMFICLFSLLVLRR55 pKa = 11.84 SYY57 pKa = 10.77 GASLIFIFFKK67 pKa = 10.61 FSMWGTSPAFNIADD81 pKa = 3.56 QLRR84 pKa = 11.84 PIVTGLEE91 pKa = 3.43 KK92 pKa = 10.21 WLNIDD97 pKa = 4.68 LMSHH101 pKa = 5.33 YY102 pKa = 10.27 EE103 pKa = 3.84 RR104 pKa = 11.84 AIRR107 pKa = 11.84 DD108 pKa = 3.62 YY109 pKa = 11.38 SLSPWSLQFLIIFIISKK126 pKa = 9.84 YY127 pKa = 8.25 FLRR130 pKa = 11.84 RR131 pKa = 11.84 QMDD134 pKa = 3.92 FAKK137 pKa = 10.55 SLLWALLISNVLGPTAGSRR156 pKa = 11.84 FVKK159 pKa = 10.39 AVTKK163 pKa = 9.51 VLPRR167 pKa = 11.84 FHH169 pKa = 7.25 LSHH172 pKa = 7.1 LSYY175 pKa = 10.99 LAMAFRR181 pKa = 11.84 RR182 pKa = 11.84 PWLRR186 pKa = 11.84 LVLEE190 pKa = 4.53 EE191 pKa = 3.99 VTPPIEE197 pKa = 3.85 DD198 pKa = 2.74 RR199 pKa = 11.84 MYY201 pKa = 10.97 RR202 pKa = 11.84 SVLALRR208 pKa = 11.84 ALKK211 pKa = 9.84 WLGAMGFMLPSDD223 pKa = 3.87 SPTARR228 pKa = 11.84 RR229 pKa = 11.84 SRR231 pKa = 11.84 IGTLSQLLQIGGKK244 pKa = 9.09 IVNDD248 pKa = 3.54 FDD250 pKa = 4.31 LPAFIRR256 pKa = 11.84 SPYY259 pKa = 9.31 QSRR262 pKa = 11.84 LTEE265 pKa = 4.01 VEE267 pKa = 4.47 TIDD270 pKa = 4.12 LMKK273 pKa = 10.93 SLGYY277 pKa = 7.18 PTNAQVQIHH286 pKa = 5.8 VPSKK290 pKa = 10.49 SGQQNKK296 pKa = 8.66 PWWIGRR302 pKa = 11.84 SDD304 pKa = 3.72 WKK306 pKa = 10.04 MHH308 pKa = 6.09 LPMFKK313 pKa = 10.58 SFIQEE318 pKa = 3.74 EE319 pKa = 4.53 LEE321 pKa = 4.31 DD322 pKa = 4.29 FRR324 pKa = 11.84 KK325 pKa = 9.97 LRR327 pKa = 11.84 NLPVFKK333 pKa = 10.52 HH334 pKa = 5.98 SYY336 pKa = 9.54 TYY338 pKa = 9.77 GTLDD342 pKa = 3.44 SQVEE346 pKa = 4.47 SISRR350 pKa = 11.84 YY351 pKa = 8.65 FYY353 pKa = 10.23 EE354 pKa = 4.92 SPVSMTLTKK363 pKa = 10.54 DD364 pKa = 3.27 DD365 pKa = 4.49 YY366 pKa = 11.59 DD367 pKa = 4.03 IVWDD371 pKa = 4.07 LVKK374 pKa = 10.63 DD375 pKa = 3.87 IYY377 pKa = 11.52 ANSQLAPISEE387 pKa = 4.99 IYY389 pKa = 10.37 RR390 pKa = 11.84 KK391 pKa = 8.61 WDD393 pKa = 2.78 KK394 pKa = 11.28 KK395 pKa = 9.84 MNVGVFASSRR405 pKa = 11.84 EE406 pKa = 3.95 KK407 pKa = 9.47 FTKK410 pKa = 10.35 FGSAKK415 pKa = 10.21 KK416 pKa = 8.88 MPRR419 pKa = 11.84 WEE421 pKa = 4.55 YY422 pKa = 8.89 IRR424 pKa = 11.84 LMGGHH429 pKa = 6.66 KK430 pKa = 10.08 KK431 pKa = 10.45 VIKK434 pKa = 9.5 QWYY437 pKa = 9.22 AFFKK441 pKa = 10.27 HH442 pKa = 5.44 YY443 pKa = 10.77 PRR445 pKa = 11.84 MEE447 pKa = 3.89 NFAQYY452 pKa = 7.87 FTKK455 pKa = 10.87 AEE457 pKa = 3.94 FLPPKK462 pKa = 9.9 KK463 pKa = 9.47 WLNDD467 pKa = 3.45 VVRR470 pKa = 11.84 TPVASMLPQYY480 pKa = 10.02 VSQMVWSGVQNHH492 pKa = 6.03 RR493 pKa = 11.84 FRR495 pKa = 11.84 PFEE498 pKa = 4.1 TPVKK502 pKa = 10.18 IGLPINGTNLSKK514 pKa = 10.35 IYY516 pKa = 10.35 ALHH519 pKa = 6.43 EE520 pKa = 4.13 VTGGKK525 pKa = 10.05 DD526 pKa = 3.28 GATHH530 pKa = 7.16 FAGDD534 pKa = 4.27 CSAFDD539 pKa = 3.55 STLTGPVLDD548 pKa = 4.55 VIKK551 pKa = 10.37 HH552 pKa = 3.84 VRR554 pKa = 11.84 KK555 pKa = 10.17 RR556 pKa = 11.84 GFKK559 pKa = 9.46 DD560 pKa = 2.95 HH561 pKa = 7.07 RR562 pKa = 11.84 AVKK565 pKa = 9.86 GNIMAGGQEE574 pKa = 3.97 LRR576 pKa = 11.84 GNN578 pKa = 3.83
Molecular weight: 66.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.129
IPC2_protein 9.297
IPC_protein 9.282
Toseland 9.926
ProMoST 9.633
Dawson 10.16
Bjellqvist 9.823
Wikipedia 10.321
Rodwell 10.555
Grimsley 10.233
Solomon 10.175
Lehninger 10.131
Nozaki 9.911
DTASelect 9.823
Thurlkill 9.999
EMBOSS 10.335
Sillero 10.058
Patrickios 9.823
IPC_peptide 10.175
IPC2_peptide 8.361
IPC2.peptide.svr19 8.273
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
970
392
578
485.0
55.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.423 ± 0.894
0.515 ± 0.148
4.227 ± 0.086
4.33 ± 0.61
5.67 ± 0.943
5.464 ± 0.063
2.474 ± 0.046
5.567 ± 1.185
6.186 ± 0.341
11.34 ± 0.992
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.093 ± 0.625
3.299 ± 0.465
5.258 ± 0.092
3.299 ± 0.141
5.258 ± 0.699
8.763 ± 0.704
5.155 ± 0.878
5.979 ± 0.218
2.887 ± 0.351
3.814 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here