Solirubrobacter pauli

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Thermoleophilia; Solirubrobacterales; Solirubrobacteraceae; Solirubrobacter

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6590 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A660LKA1|A0A660LKA1_9ACTN Protein-glutamate O-methyltransferase OS=Solirubrobacter pauli OX=166793 GN=C8N24_3799 PE=4 SV=1
MM1 pKa = 7.1STDD4 pKa = 3.52TDD6 pKa = 3.84RR7 pKa = 11.84QWICEE12 pKa = 4.23SCGFIYY18 pKa = 10.53DD19 pKa = 4.23PEE21 pKa = 5.34EE22 pKa = 4.39GDD24 pKa = 3.43PDD26 pKa = 3.89GGIDD30 pKa = 3.57EE31 pKa = 4.59GTAFEE36 pKa = 6.05DD37 pKa = 4.77IPKK40 pKa = 10.35DD41 pKa = 3.37WYY43 pKa = 11.22CPVCGARR50 pKa = 11.84KK51 pKa = 9.67ADD53 pKa = 4.28FSPFEE58 pKa = 4.08GG59 pKa = 3.96

Molecular weight: 6.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A660LC04|A0A660LC04_9ACTN Bcr/CflA family efflux transporter OS=Solirubrobacter pauli OX=166793 GN=C8N24_0375 PE=3 SV=1
MM1 pKa = 6.99QRR3 pKa = 11.84QPAAKK8 pKa = 8.99KK9 pKa = 9.32PAMKK13 pKa = 10.34RR14 pKa = 11.84PLLPPKK20 pKa = 10.02RR21 pKa = 11.84APRR24 pKa = 11.84PALKK28 pKa = 10.19PIARR32 pKa = 11.84QMPKK36 pKa = 8.57QQHH39 pKa = 5.38RR40 pKa = 11.84GGRR43 pKa = 3.54

Molecular weight: 4.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6590

0

6590

2231201

29

10614

338.6

36.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.17 ± 0.051

0.727 ± 0.009

6.056 ± 0.024

5.816 ± 0.04

2.987 ± 0.017

9.207 ± 0.044

2.02 ± 0.016

3.704 ± 0.022

2.255 ± 0.026

10.224 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.599 ± 0.014

1.971 ± 0.025

5.771 ± 0.03

2.693 ± 0.016

7.824 ± 0.047

4.945 ± 0.026

6.189 ± 0.049

8.519 ± 0.026

1.369 ± 0.013

1.954 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski