Leifsonia sp. BK543

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leifsonia; unclassified Leifsonia

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3055 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q7PR06|A0A4Q7PR06_9MICO 6 7-dimethyl-8-ribityllumazine synthase OS=Leifsonia sp. BK543 OX=2512174 GN=ribH PE=3 SV=1
MM1 pKa = 7.59PRR3 pKa = 11.84TRR5 pKa = 11.84HH6 pKa = 5.45RR7 pKa = 11.84RR8 pKa = 11.84TVLLVLLGAAAFALAGCAADD28 pKa = 3.8PEE30 pKa = 4.89VVEE33 pKa = 4.79SAPSASPTPTEE44 pKa = 4.11APAPTSVRR52 pKa = 11.84PDD54 pKa = 2.83ARR56 pKa = 11.84IALTCDD62 pKa = 3.1QLVPTAAADD71 pKa = 3.96GFGAPALHH79 pKa = 6.37GVVDD83 pKa = 5.26LDD85 pKa = 4.04SAAPAQAGWTWCSWGAVGQLRR106 pKa = 11.84LEE108 pKa = 4.29LVVVPDD114 pKa = 4.24GPIDD118 pKa = 4.02DD119 pKa = 4.94SVNEE123 pKa = 4.07PCVLFPSGAGCTAQVSVGSTQVWVTAGYY151 pKa = 9.41PGGTPLADD159 pKa = 3.9ADD161 pKa = 3.61QSFPHH166 pKa = 7.37IVEE169 pKa = 3.93NVAAILAEE177 pKa = 4.34TTVAPAITPQRR188 pKa = 11.84TVPAGAVSGLDD199 pKa = 3.44PATAAGWFGVDD210 pKa = 3.47PDD212 pKa = 3.91GGIPLGVPQEE222 pKa = 4.21ILGDD226 pKa = 3.37AAYY229 pKa = 9.96RR230 pKa = 11.84QTASEE235 pKa = 3.98TAAWGTEE242 pKa = 3.89EE243 pKa = 4.71LSWVQVDD250 pKa = 4.64LLPGGGWAAAGFPDD264 pKa = 5.22APVAPLQQPSAFEE277 pKa = 3.96PTEE280 pKa = 3.96VPGFADD286 pKa = 3.59AALEE290 pKa = 4.16TSDD293 pKa = 6.07GYY295 pKa = 10.78QQLCGAIGTDD305 pKa = 4.16LVCVAAYY312 pKa = 9.88GVDD315 pKa = 3.38PALFLQGAAALAAEE329 pKa = 4.9LGG331 pKa = 3.79

Molecular weight: 33.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q7PRJ4|A0A4Q7PRJ4_9MICO 1-acyl-sn-glycerol-3-phosphate acyltransferase OS=Leifsonia sp. BK543 OX=2512174 GN=EV591_1843 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3055

0

3055

1003576

29

2789

328.5

35.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.74 ± 0.065

0.445 ± 0.01

6.283 ± 0.037

5.569 ± 0.044

3.199 ± 0.027

9.092 ± 0.04

1.842 ± 0.021

4.599 ± 0.033

1.817 ± 0.031

10.293 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.631 ± 0.016

1.901 ± 0.024

5.563 ± 0.036

2.635 ± 0.024

7.123 ± 0.047

5.412 ± 0.034

6.093 ± 0.055

9.191 ± 0.037

1.557 ± 0.021

2.015 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski