Amino acid dipepetide frequency for Leifsonia sp. BK543

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.159AlaAla: 21.159 ± 0.226
0.586AlaCys: 0.586 ± 0.027
8.663AlaAsp: 8.663 ± 0.11
8.223AlaGlu: 8.223 ± 0.104
4.22AlaPhe: 4.22 ± 0.073
12.406AlaGly: 12.406 ± 0.142
2.405AlaHis: 2.405 ± 0.052
6.549AlaIle: 6.549 ± 0.099
2.48AlaLys: 2.48 ± 0.065
14.25AlaLeu: 14.25 ± 0.148
2.441AlaMet: 2.441 ± 0.051
2.35AlaAsn: 2.35 ± 0.052
6.94AlaPro: 6.94 ± 0.119
3.446AlaGln: 3.446 ± 0.071
9.262AlaArg: 9.262 ± 0.143
7.783AlaSer: 7.783 ± 0.099
7.692AlaThr: 7.692 ± 0.107
12.164AlaVal: 12.164 ± 0.123
1.979AlaTrp: 1.979 ± 0.053
2.402AlaTyr: 2.402 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
0.572CysAla: 0.572 ± 0.024
0.04CysCys: 0.04 ± 0.006
0.285CysAsp: 0.285 ± 0.02
0.229CysGlu: 0.229 ± 0.016
0.145CysPhe: 0.145 ± 0.012
0.492CysGly: 0.492 ± 0.025
0.093CysHis: 0.093 ± 0.011
0.177CysIle: 0.177 ± 0.014
0.038CysLys: 0.038 ± 0.007
0.4CysLeu: 0.4 ± 0.023
0.067CysMet: 0.067 ± 0.007
0.094CysAsn: 0.094 ± 0.01
0.246CysPro: 0.246 ± 0.019
0.097CysGln: 0.097 ± 0.011
0.278CysArg: 0.278 ± 0.018
0.285CysSer: 0.285 ± 0.017
0.338CysThr: 0.338 ± 0.022
0.402CysVal: 0.402 ± 0.021
0.07CysTrp: 0.07 ± 0.008
0.107CysTyr: 0.107 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
9.642AspAla: 9.642 ± 0.124
0.222AspCys: 0.222 ± 0.018
4.56AspAsp: 4.56 ± 0.077
4.485AspGlu: 4.485 ± 0.086
1.797AspPhe: 1.797 ± 0.041
6.467AspGly: 6.467 ± 0.106
1.158AspHis: 1.158 ± 0.035
2.397AspIle: 2.397 ± 0.051
0.889AspLys: 0.889 ± 0.037
5.807AspLeu: 5.807 ± 0.083
0.676AspMet: 0.676 ± 0.025
0.903AspAsn: 0.903 ± 0.033
4.66AspPro: 4.66 ± 0.061
1.494AspGln: 1.494 ± 0.035
4.579AspArg: 4.579 ± 0.089
2.464AspSer: 2.464 ± 0.049
3.006AspThr: 3.006 ± 0.064
5.18AspVal: 5.18 ± 0.075
1.029AspTrp: 1.029 ± 0.033
1.41AspTyr: 1.41 ± 0.043
0.0AspXaa: 0.0 ± 0.0
Glu
6.901GluAla: 6.901 ± 0.09
0.223GluCys: 0.223 ± 0.016
2.177GluAsp: 2.177 ± 0.049
2.74GluGlu: 2.74 ± 0.059
1.858GluPhe: 1.858 ± 0.044
4.011GluGly: 4.011 ± 0.072
1.364GluHis: 1.364 ± 0.036
2.468GluIle: 2.468 ± 0.05
1.308GluLys: 1.308 ± 0.044
7.58GluLeu: 7.58 ± 0.108
0.782GluMet: 0.782 ± 0.03
1.186GluAsn: 1.186 ± 0.033
3.165GluPro: 3.165 ± 0.057
2.127GluGln: 2.127 ± 0.048
5.071GluArg: 5.071 ± 0.092
2.774GluSer: 2.774 ± 0.058
3.036GluThr: 3.036 ± 0.057
4.954GluVal: 4.954 ± 0.068
0.799GluTrp: 0.799 ± 0.027
1.162GluTyr: 1.162 ± 0.034
0.0GluXaa: 0.0 ± 0.0
Phe
4.562PheAla: 4.562 ± 0.068
0.173PheCys: 0.173 ± 0.013
2.345PheAsp: 2.345 ± 0.047
1.772PheGlu: 1.772 ± 0.039
1.098PhePhe: 1.098 ± 0.039
3.587PheGly: 3.587 ± 0.06
0.504PheHis: 0.504 ± 0.021
1.235PheIle: 1.235 ± 0.042
0.388PheLys: 0.388 ± 0.021
2.952PheLeu: 2.952 ± 0.062
0.451PheMet: 0.451 ± 0.019
0.554PheAsn: 0.554 ± 0.027
1.52PhePro: 1.52 ± 0.042
0.802PheGln: 0.802 ± 0.026
1.925PheArg: 1.925 ± 0.045
1.82PheSer: 1.82 ± 0.044
2.223PheThr: 2.223 ± 0.051
2.89PheVal: 2.89 ± 0.052
0.55PheTrp: 0.55 ± 0.028
0.642PheTyr: 0.642 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
11.072GlyAla: 11.072 ± 0.131
0.581GlyCys: 0.581 ± 0.026
5.541GlyAsp: 5.541 ± 0.081
4.976GlyGlu: 4.976 ± 0.073
3.376GlyPhe: 3.376 ± 0.064
7.976GlyGly: 7.976 ± 0.123
1.739GlyHis: 1.739 ± 0.043
4.962GlyIle: 4.962 ± 0.082
1.928GlyLys: 1.928 ± 0.053
9.037GlyLeu: 9.037 ± 0.11
1.905GlyMet: 1.905 ± 0.039
1.648GlyAsn: 1.648 ± 0.044
3.881GlyPro: 3.881 ± 0.07
2.259GlyGln: 2.259 ± 0.055
6.252GlyArg: 6.252 ± 0.089
5.498GlySer: 5.498 ± 0.079
5.78GlyThr: 5.78 ± 0.104
8.36GlyVal: 8.36 ± 0.097
1.788GlyTrp: 1.788 ± 0.045
2.359GlyTyr: 2.359 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
2.21HisAla: 2.21 ± 0.051
0.083HisCys: 0.083 ± 0.01
1.275HisAsp: 1.275 ± 0.036
1.1HisGlu: 1.1 ± 0.037
0.516HisPhe: 0.516 ± 0.024
1.845HisGly: 1.845 ± 0.043
0.525HisHis: 0.525 ± 0.027
0.675HisIle: 0.675 ± 0.028
0.224HisLys: 0.224 ± 0.014
2.053HisLeu: 2.053 ± 0.051
0.264HisMet: 0.264 ± 0.017
0.331HisAsn: 0.331 ± 0.018
1.472HisPro: 1.472 ± 0.044
0.447HisGln: 0.447 ± 0.02
1.44HisArg: 1.44 ± 0.038
0.821HisSer: 0.821 ± 0.031
0.889HisThr: 0.889 ± 0.032
1.528HisVal: 1.528 ± 0.038
0.303HisTrp: 0.303 ± 0.018
0.42HisTyr: 0.42 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
7.16IleAla: 7.16 ± 0.092
0.198IleCys: 0.198 ± 0.014
3.498IleAsp: 3.498 ± 0.06
2.736IleGlu: 2.736 ± 0.053
1.196IlePhe: 1.196 ± 0.04
4.686IleGly: 4.686 ± 0.079
0.641IleHis: 0.641 ± 0.028
1.798IleIle: 1.798 ± 0.053
0.741IleLys: 0.741 ± 0.03
3.761IleLeu: 3.761 ± 0.071
0.585IleMet: 0.585 ± 0.024
0.872IleAsn: 0.872 ± 0.033
2.616IlePro: 2.616 ± 0.05
1.031IleGln: 1.031 ± 0.036
3.138IleArg: 3.138 ± 0.062
2.287IleSer: 2.287 ± 0.047
2.808IleThr: 2.808 ± 0.056
4.875IleVal: 4.875 ± 0.07
0.578IleTrp: 0.578 ± 0.027
0.79IleTyr: 0.79 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
2.263LysAla: 2.263 ± 0.059
0.045LysCys: 0.045 ± 0.007
0.926LysAsp: 0.926 ± 0.037
0.847LysGlu: 0.847 ± 0.03
0.461LysPhe: 0.461 ± 0.021
1.369LysGly: 1.369 ± 0.043
0.446LysHis: 0.446 ± 0.024
0.779LysIle: 0.779 ± 0.031
0.734LysLys: 0.734 ± 0.035
1.901LysLeu: 1.901 ± 0.047
0.332LysMet: 0.332 ± 0.019
0.474LysAsn: 0.474 ± 0.024
1.143LysPro: 1.143 ± 0.045
0.599LysGln: 0.599 ± 0.027
1.397LysArg: 1.397 ± 0.043
1.021LysSer: 1.021 ± 0.037
1.17LysThr: 1.17 ± 0.039
1.661LysVal: 1.661 ± 0.054
0.229LysTrp: 0.229 ± 0.015
0.375LysTyr: 0.375 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
15.524LeuAla: 15.524 ± 0.163
0.436LeuCys: 0.436 ± 0.022
7.088LeuAsp: 7.088 ± 0.115
5.367LeuGlu: 5.367 ± 0.067
2.975LeuPhe: 2.975 ± 0.06
9.721LeuGly: 9.721 ± 0.119
1.728LeuHis: 1.728 ± 0.041
4.504LeuIle: 4.504 ± 0.077
1.614LeuLys: 1.614 ± 0.045
10.77LeuLeu: 10.77 ± 0.178
1.455LeuMet: 1.455 ± 0.042
1.721LeuAsn: 1.721 ± 0.038
5.779LeuPro: 5.779 ± 0.081
2.493LeuGln: 2.493 ± 0.051
7.059LeuArg: 7.059 ± 0.112
5.417LeuSer: 5.417 ± 0.072
6.172LeuThr: 6.172 ± 0.094
10.131LeuVal: 10.131 ± 0.119
1.272LeuTrp: 1.272 ± 0.034
1.701LeuTyr: 1.701 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
1.963MetAla: 1.963 ± 0.044
0.102MetCys: 0.102 ± 0.011
0.653MetAsp: 0.653 ± 0.027
0.588MetGlu: 0.588 ± 0.022
0.487MetPhe: 0.487 ± 0.021
1.18MetGly: 1.18 ± 0.034
0.288MetHis: 0.288 ± 0.016
0.798MetIle: 0.798 ± 0.027
0.355MetLys: 0.355 ± 0.019
1.734MetLeu: 1.734 ± 0.047
0.254MetMet: 0.254 ± 0.016
0.434MetAsn: 0.434 ± 0.021
1.08MetPro: 1.08 ± 0.027
0.468MetGln: 0.468 ± 0.02
1.269MetArg: 1.269 ± 0.038
1.327MetSer: 1.327 ± 0.036
1.64MetThr: 1.64 ± 0.045
1.244MetVal: 1.244 ± 0.035
0.197MetTrp: 0.197 ± 0.014
0.251MetTyr: 0.251 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
2.381AsnAla: 2.381 ± 0.055
0.119AsnCys: 0.119 ± 0.012
1.096AsnAsp: 1.096 ± 0.044
0.874AsnGlu: 0.874 ± 0.032
0.625AsnPhe: 0.625 ± 0.03
1.883AsnGly: 1.883 ± 0.05
0.349AsnHis: 0.349 ± 0.02
0.794AsnIle: 0.794 ± 0.034
0.361AsnLys: 0.361 ± 0.022
1.859AsnLeu: 1.859 ± 0.048
0.268AsnMet: 0.268 ± 0.016
0.491AsnAsn: 0.491 ± 0.025
1.608AsnPro: 1.608 ± 0.043
0.499AsnGln: 0.499 ± 0.024
1.214AsnArg: 1.214 ± 0.035
0.982AsnSer: 0.982 ± 0.043
1.113AsnThr: 1.113 ± 0.045
1.646AsnVal: 1.646 ± 0.042
0.348AsnTrp: 0.348 ± 0.021
0.497AsnTyr: 0.497 ± 0.024
0.0AsnXaa: 0.0 ± 0.0
Pro
7.793ProAla: 7.793 ± 0.112
0.173ProCys: 0.173 ± 0.013
4.29ProAsp: 4.29 ± 0.071
3.869ProGlu: 3.869 ± 0.066
1.817ProPhe: 1.817 ± 0.045
5.232ProGly: 5.232 ± 0.086
1.055ProHis: 1.055 ± 0.038
2.208ProIle: 2.208 ± 0.055
0.979ProLys: 0.979 ± 0.04
4.976ProLeu: 4.976 ± 0.083
0.835ProMet: 0.835 ± 0.027
1.077ProAsn: 1.077 ± 0.034
2.495ProPro: 2.495 ± 0.082
1.551ProGln: 1.551 ± 0.049
3.405ProArg: 3.405 ± 0.066
3.059ProSer: 3.059 ± 0.056
3.57ProThr: 3.57 ± 0.064
5.15ProVal: 5.15 ± 0.088
0.948ProTrp: 0.948 ± 0.032
1.147ProTyr: 1.147 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
3.268GlnAla: 3.268 ± 0.062
0.099GlnCys: 0.099 ± 0.01
1.088GlnAsp: 1.088 ± 0.036
1.221GlnGlu: 1.221 ± 0.033
0.835GlnPhe: 0.835 ± 0.03
1.844GlnGly: 1.844 ± 0.051
0.534GlnHis: 0.534 ± 0.023
1.188GlnIle: 1.188 ± 0.034
0.658GlnLys: 0.658 ± 0.027
3.482GlnLeu: 3.482 ± 0.065
0.406GlnMet: 0.406 ± 0.022
0.618GlnAsn: 0.618 ± 0.025
1.524GlnPro: 1.524 ± 0.053
1.152GlnGln: 1.152 ± 0.047
2.183GlnArg: 2.183 ± 0.053
1.262GlnSer: 1.262 ± 0.042
1.485GlnThr: 1.485 ± 0.042
2.471GlnVal: 2.471 ± 0.051
0.411GlnTrp: 0.411 ± 0.022
0.62GlnTyr: 0.62 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
9.004ArgAla: 9.004 ± 0.127
0.252ArgCys: 0.252 ± 0.018
4.269ArgAsp: 4.269 ± 0.075
4.315ArgGlu: 4.315 ± 0.081
2.535ArgPhe: 2.535 ± 0.052
5.512ArgGly: 5.512 ± 0.085
1.406ArgHis: 1.406 ± 0.041
3.949ArgIle: 3.949 ± 0.064
1.237ArgLys: 1.237 ± 0.043
7.129ArgLeu: 7.129 ± 0.114
1.677ArgMet: 1.677 ± 0.038
1.286ArgAsn: 1.286 ± 0.037
3.626ArgPro: 3.626 ± 0.066
1.858ArgGln: 1.858 ± 0.04
6.35ArgArg: 6.35 ± 0.112
3.736ArgSer: 3.736 ± 0.062
4.106ArgThr: 4.106 ± 0.061
6.149ArgVal: 6.149 ± 0.095
1.24ArgTrp: 1.24 ± 0.039
1.593ArgTyr: 1.593 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
6.958SerAla: 6.958 ± 0.084
0.231SerCys: 0.231 ± 0.015
3.241SerAsp: 3.241 ± 0.061
2.628SerGlu: 2.628 ± 0.057
1.86SerPhe: 1.86 ± 0.041
5.918SerGly: 5.918 ± 0.089
0.93SerHis: 0.93 ± 0.033
2.605SerIle: 2.605 ± 0.054
0.938SerLys: 0.938 ± 0.032
5.061SerLeu: 5.061 ± 0.083
1.002SerMet: 1.002 ± 0.032
1.069SerAsn: 1.069 ± 0.039
2.983SerPro: 2.983 ± 0.059
1.277SerGln: 1.277 ± 0.035
3.661SerArg: 3.661 ± 0.068
3.212SerSer: 3.212 ± 0.06
3.711SerThr: 3.711 ± 0.067
4.59SerVal: 4.59 ± 0.068
0.977SerTrp: 0.977 ± 0.034
1.266SerTyr: 1.266 ± 0.04
0.0SerXaa: 0.0 ± 0.0
Thr
7.917ThrAla: 7.917 ± 0.103
0.263ThrCys: 0.263 ± 0.024
3.54ThrAsp: 3.54 ± 0.067
2.905ThrGlu: 2.905 ± 0.06
1.924ThrPhe: 1.924 ± 0.04
5.979ThrGly: 5.979 ± 0.088
1.036ThrHis: 1.036 ± 0.032
3.07ThrIle: 3.07 ± 0.065
1.052ThrLys: 1.052 ± 0.038
6.144ThrLeu: 6.144 ± 0.079
0.96ThrMet: 0.96 ± 0.032
1.184ThrAsn: 1.184 ± 0.043
4.113ThrPro: 4.113 ± 0.083
1.45ThrGln: 1.45 ± 0.036
3.967ThrArg: 3.967 ± 0.068
3.491ThrSer: 3.491 ± 0.072
4.041ThrThr: 4.041 ± 0.087
5.898ThrVal: 5.898 ± 0.11
0.959ThrTrp: 0.959 ± 0.034
1.039ThrTyr: 1.039 ± 0.04
0.0ThrXaa: 0.0 ± 0.0
Val
12.738ValAla: 12.738 ± 0.13
0.44ValCys: 0.44 ± 0.021
6.121ValAsp: 6.121 ± 0.081
5.063ValGlu: 5.063 ± 0.083
2.937ValPhe: 2.937 ± 0.05
7.628ValGly: 7.628 ± 0.094
1.613ValHis: 1.613 ± 0.04
4.401ValIle: 4.401 ± 0.062
1.601ValLys: 1.601 ± 0.039
9.841ValLeu: 9.841 ± 0.134
1.366ValMet: 1.366 ± 0.037
1.805ValAsn: 1.805 ± 0.051
4.898ValPro: 4.898 ± 0.073
2.16ValGln: 2.16 ± 0.053
5.912ValArg: 5.912 ± 0.078
4.883ValSer: 4.883 ± 0.088
5.949ValThr: 5.949 ± 0.111
9.746ValVal: 9.746 ± 0.13
1.186ValTrp: 1.186 ± 0.036
1.618ValTyr: 1.618 ± 0.041
0.0ValXaa: 0.0 ± 0.0
Trp
1.787TrpAla: 1.787 ± 0.043
0.085TrpCys: 0.085 ± 0.008
0.849TrpAsp: 0.849 ± 0.029
0.672TrpGlu: 0.672 ± 0.029
0.618TrpPhe: 0.618 ± 0.024
1.237TrpGly: 1.237 ± 0.043
0.315TrpHis: 0.315 ± 0.017
0.74TrpIle: 0.74 ± 0.028
0.305TrpLys: 0.305 ± 0.018
1.756TrpLeu: 1.756 ± 0.043
0.363TrpMet: 0.363 ± 0.021
0.474TrpAsn: 0.474 ± 0.025
0.777TrpPro: 0.777 ± 0.028
0.559TrpGln: 0.559 ± 0.02
1.18TrpArg: 1.18 ± 0.035
0.913TrpSer: 0.913 ± 0.03
0.977TrpThr: 0.977 ± 0.032
1.269TrpVal: 1.269 ± 0.034
0.377TrpTrp: 0.377 ± 0.02
0.324TrpTyr: 0.324 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.523TyrAla: 2.523 ± 0.052
0.102TyrCys: 0.102 ± 0.011
1.32TyrAsp: 1.32 ± 0.035
1.08TyrGlu: 1.08 ± 0.033
0.711TyrPhe: 0.711 ± 0.028
1.945TyrGly: 1.945 ± 0.043
0.291TyrHis: 0.291 ± 0.018
0.698TyrIle: 0.698 ± 0.026
0.344TyrLys: 0.344 ± 0.022
2.436TyrLeu: 2.436 ± 0.052
0.223TyrMet: 0.223 ± 0.015
0.437TyrAsn: 0.437 ± 0.024
1.102TyrPro: 1.102 ± 0.037
0.576TyrGln: 0.576 ± 0.027
1.688TyrArg: 1.688 ± 0.045
1.082TyrSer: 1.082 ± 0.037
1.226TyrThr: 1.226 ± 0.042
1.601TyrVal: 1.601 ± 0.045
0.337TyrTrp: 0.337 ± 0.021
0.431TyrTyr: 0.431 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3055 proteins (1003576 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski