Streptomyces himastatinicus ATCC 53653

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces violaceusniger group; Streptomyces himastatinicus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9177 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9WAD5|D9WAD5_9ACTN Uncharacterized protein OS=Streptomyces himastatinicus ATCC 53653 OX=457427 GN=SSOG_04645 PE=4 SV=1
LL1 pKa = 6.4RR2 pKa = 11.84TAARR6 pKa = 11.84AYY8 pKa = 7.83VTNRR12 pKa = 11.84DD13 pKa = 3.32SDD15 pKa = 4.22TVSVISTSANTVIATIDD32 pKa = 3.4VGDD35 pKa = 4.03YY36 pKa = 8.97PQGVAVAPDD45 pKa = 3.88GSRR48 pKa = 11.84VYY50 pKa = 9.31VTNSGNGDD58 pKa = 3.73GVSVIDD64 pKa = 3.95TGTHH68 pKa = 4.3TVTATVATGNHH79 pKa = 6.14PNAVAVTPDD88 pKa = 3.17GTRR91 pKa = 11.84VYY93 pKa = 10.4IADD96 pKa = 4.4AGDD99 pKa = 4.28DD100 pKa = 3.92SLSVISTSTNTVTATISVGDD120 pKa = 4.06NPEE123 pKa = 4.64GIAVAPDD130 pKa = 3.26GTRR133 pKa = 11.84AYY135 pKa = 8.68VTLYY139 pKa = 10.56NSASVAVVDD148 pKa = 4.82ISANTVTDD156 pKa = 4.34TIGVGTLPYY165 pKa = 10.12GVAVTADD172 pKa = 3.35GTRR175 pKa = 11.84AYY177 pKa = 8.64VTNSDD182 pKa = 3.66GYY184 pKa = 9.85ANTVSVIDD192 pKa = 3.8TSDD195 pKa = 3.11NTVIDD200 pKa = 4.52TIDD203 pKa = 3.4VGTFPYY209 pKa = 10.69GVATAVVRR217 pKa = 11.84APKK220 pKa = 8.25PTCANATPTITGTNGSDD237 pKa = 3.87NITGTPGNDD246 pKa = 3.33AIFASGGNDD255 pKa = 3.23VVDD258 pKa = 3.93GAGGNDD264 pKa = 3.95LVCGGTGHH272 pKa = 7.44DD273 pKa = 3.88VLRR276 pKa = 11.84GGTGDD281 pKa = 4.11DD282 pKa = 4.06RR283 pKa = 11.84IEE285 pKa = 4.63GGDD288 pKa = 3.85GNDD291 pKa = 3.42ILLGGDD297 pKa = 3.49GDD299 pKa = 4.11DD300 pKa = 4.59ALFGEE305 pKa = 5.05AGNDD309 pKa = 3.37VLVGNAGDD317 pKa = 3.73NTNDD321 pKa = 3.23GGAGANICANPATGPGCC338 pKa = 3.95

Molecular weight: 33.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9W929|D9W929_9ACTN Putative membrane protein OS=Streptomyces himastatinicus ATCC 53653 OX=457427 GN=SSOG_06525 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 11.06GRR40 pKa = 11.84GRR42 pKa = 11.84LSAA45 pKa = 4.4

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9177

0

9177

3045779

20

7593

331.9

35.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.466 ± 0.036

0.836 ± 0.008

6.001 ± 0.02

5.563 ± 0.022

2.709 ± 0.013

9.635 ± 0.026

2.411 ± 0.012

3.204 ± 0.016

2.064 ± 0.025

10.185 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.806 ± 0.01

1.728 ± 0.012

6.26 ± 0.023

2.738 ± 0.015

8.426 ± 0.03

5.11 ± 0.02

6.117 ± 0.02

8.167 ± 0.029

1.529 ± 0.01

2.046 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski