Aspergillus terreus (strain NIH 2624 / FGSC A1156)
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10417 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q0CTY6|Q0CTY6_ASPTN [RNA-polymerase]-subunit kinase OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=ATEG_02848 PE=3 SV=1
MM1 pKa = 7.05 QFSKK5 pKa = 11.22 SFMLLAALTTGALALPQKK23 pKa = 10.3 RR24 pKa = 11.84 DD25 pKa = 2.89 WNTAGFGASTASSGSDD41 pKa = 2.92 VTYY44 pKa = 10.33 QGNVGNPWGSNIIEE58 pKa = 4.25 VSSSDD63 pKa = 3.26 AASYY67 pKa = 10.71 KK68 pKa = 9.27 YY69 pKa = 8.98 TLEE72 pKa = 3.67 ITGQNSEE79 pKa = 3.64 PWQIVFWNKK88 pKa = 10.14 YY89 pKa = 9.75 GPDD92 pKa = 3.42 GLMDD96 pKa = 3.71 GWFGNSALTLTLNAGEE112 pKa = 4.29 TKK114 pKa = 10.57 YY115 pKa = 11.01 VAFDD119 pKa = 4.29 DD120 pKa = 4.08 DD121 pKa = 4.51 TNGGFAAGPGSVPTANGEE139 pKa = 4.09 WASTWGEE146 pKa = 3.81 FDD148 pKa = 4.98 FGSTTNGGWSGFDD161 pKa = 2.97 VSAIVAQNTGQTVQGMQMCDD181 pKa = 3.09 KK182 pKa = 11.01 ASGVCSTITPNAATVDD198 pKa = 3.73 NAYY201 pKa = 6.81 TTAEE205 pKa = 4.04 TDD207 pKa = 2.75 IGGIGGNISGGGQVQLTAVIDD228 pKa = 3.96 YY229 pKa = 10.66 QGG231 pKa = 2.91
Molecular weight: 23.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.757
IPC_protein 3.732
Toseland 3.516
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.694
Rodwell 3.567
Grimsley 3.427
Solomon 3.719
Lehninger 3.681
Nozaki 3.859
DTASelect 4.113
Thurlkill 3.579
EMBOSS 3.706
Sillero 3.859
Patrickios 0.896
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.768
Protein with the highest isoelectric point:
>tr|Q0CN60|Q0CN60_ASPTN GTP-binding protein ypt3 OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=ATEG_04874 PE=4 SV=1
MM1 pKa = 7.38 LTTTASSRR9 pKa = 11.84 KK10 pKa = 8.93 RR11 pKa = 11.84 RR12 pKa = 11.84 TISRR16 pKa = 11.84 TASTTNNLLSGYY28 pKa = 9.66 RR29 pKa = 11.84 PFISAVPSSEE39 pKa = 3.96 STSNSLVPGGQSGSTAHH56 pKa = 7.02 ACTSLTTTTRR66 pKa = 11.84 RR67 pKa = 11.84 GLRR70 pKa = 11.84 ARR72 pKa = 11.84 GCACADD78 pKa = 3.25 ARR80 pKa = 11.84 SVGSTSSPSSTVLSTFTVSVRR101 pKa = 11.84 SYY103 pKa = 9.6 PSAVAKK109 pKa = 9.44 TVVAIAAFATTASSLGSCAARR130 pKa = 11.84 AAKK133 pKa = 10.2 ACTEE137 pKa = 3.99 AKK139 pKa = 10.08 EE140 pKa = 4.23 EE141 pKa = 4.13 RR142 pKa = 11.84 SSGQTSQMPGRR153 pKa = 11.84 PVEE156 pKa = 3.86 VSMEE160 pKa = 3.75 VRR162 pKa = 11.84 AASPAEE168 pKa = 3.74 RR169 pKa = 11.84 GMEE172 pKa = 4.05 VVVVMDD178 pKa = 4.03 RR179 pKa = 11.84 VV180 pKa = 3.23
Molecular weight: 18.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.472
IPC_protein 10.438
Toseland 10.657
ProMoST 10.452
Dawson 10.73
Bjellqvist 10.526
Wikipedia 10.979
Rodwell 10.774
Grimsley 10.774
Solomon 10.921
Lehninger 10.877
Nozaki 10.687
DTASelect 10.496
Thurlkill 10.657
EMBOSS 11.067
Sillero 10.687
Patrickios 10.54
IPC_peptide 10.921
IPC2_peptide 9.97
IPC2.peptide.svr19 8.609
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10417
0
10417
5198365
48
5842
499.0
55.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.738 ± 0.023
1.292 ± 0.009
5.726 ± 0.018
6.024 ± 0.026
3.741 ± 0.015
6.855 ± 0.024
2.477 ± 0.01
4.821 ± 0.016
4.414 ± 0.021
9.119 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.182 ± 0.008
3.486 ± 0.012
6.158 ± 0.026
3.969 ± 0.016
6.295 ± 0.02
8.105 ± 0.025
5.889 ± 0.015
6.326 ± 0.017
1.499 ± 0.008
2.884 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here