Spiroplasma syrphidicola EA-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae; Spiroplasma; Spiroplasma syrphidicola

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1006 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4U5P1|R4U5P1_9MOLU Dipeptidase PepV OS=Spiroplasma syrphidicola EA-1 OX=1276229 GN=ytjP PE=4 SV=1
MM1 pKa = 7.45NNGFYY6 pKa = 10.39ILPNDD11 pKa = 3.12EE12 pKa = 4.53GYY14 pKa = 10.66LVKK17 pKa = 10.77SHH19 pKa = 6.83DD20 pKa = 3.97TNGDD24 pKa = 3.11VALFRR29 pKa = 11.84SRR31 pKa = 11.84RR32 pKa = 11.84DD33 pKa = 3.15AEE35 pKa = 4.33VFISALTTPQHH46 pKa = 5.25GMGYY50 pKa = 8.24QAPYY54 pKa = 8.62PGYY57 pKa = 9.96MPGQPMMPMYY67 pKa = 9.91PPQSSPQVFFIQQPAAPSPYY87 pKa = 9.46PPAPYY92 pKa = 9.55PFYY95 pKa = 11.02NSYY98 pKa = 9.32PAPGMMVPPMPMPGCGCQHH117 pKa = 6.42GFDD120 pKa = 4.46HH121 pKa = 6.37QHH123 pKa = 5.07QAGNNVNNGNNQNNRR138 pKa = 11.84QGQGNFDD145 pKa = 3.66NNVNNNANQNDD156 pKa = 4.24YY157 pKa = 11.37NLNQPPYY164 pKa = 9.32YY165 pKa = 9.19EE166 pKa = 4.19QEE168 pKa = 4.03FMADD172 pKa = 3.58PVYY175 pKa = 10.74LGQDD179 pKa = 3.04QNYY182 pKa = 8.93DD183 pKa = 3.12QNYY186 pKa = 9.48NYY188 pKa = 10.55NNEE191 pKa = 4.12ANYY194 pKa = 10.77SQTDD198 pKa = 3.64NNGTDD203 pKa = 3.62YY204 pKa = 11.71NDD206 pKa = 4.76GNNQTQDD213 pKa = 3.18NEE215 pKa = 4.19YY216 pKa = 10.44SQPEE220 pKa = 4.3GQAEE224 pKa = 3.99DD225 pKa = 3.68TNINNFGQEE234 pKa = 3.5NSQYY238 pKa = 10.88QEE240 pKa = 3.96TANQDD245 pKa = 3.38NNFEE249 pKa = 4.46NIIGDD254 pKa = 3.89SGDD257 pKa = 3.37MFDD260 pKa = 6.3FIDD263 pKa = 4.08NEE265 pKa = 4.43PVSSEE270 pKa = 3.8SGEE273 pKa = 3.92LSKK276 pKa = 11.11RR277 pKa = 11.84QLKK280 pKa = 10.22KK281 pKa = 10.26LAKK284 pKa = 9.29QEE286 pKa = 3.84KK287 pKa = 9.53KK288 pKa = 10.14AAKK291 pKa = 9.08IQNKK295 pKa = 8.99VEE297 pKa = 3.96QKK299 pKa = 9.85NAKK302 pKa = 9.65KK303 pKa = 9.03MAKK306 pKa = 9.49LGKK309 pKa = 9.66YY310 pKa = 8.94DD311 pKa = 3.71QYY313 pKa = 12.19DD314 pKa = 3.53NLEE317 pKa = 4.54DD318 pKa = 4.04LDD320 pKa = 4.54SFVEE324 pKa = 4.2

Molecular weight: 36.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4U2R2|R4U2R2_9MOLU Uncharacterized protein OS=Spiroplasma syrphidicola EA-1 OX=1276229 GN=SSYRP_v1c00510 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.14IKK11 pKa = 10.33HH12 pKa = 5.53KK13 pKa = 9.07RR14 pKa = 11.84THH16 pKa = 5.84GFRR19 pKa = 11.84ARR21 pKa = 11.84MQSATGRR28 pKa = 11.84KK29 pKa = 8.39LLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.5GRR39 pKa = 11.84KK40 pKa = 8.78VLSAA44 pKa = 4.05

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1006

0

1006

333167

34

1451

331.2

37.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.976 ± 0.082

0.692 ± 0.02

5.089 ± 0.055

6.023 ± 0.081

5.175 ± 0.066

5.398 ± 0.075

1.524 ± 0.03

9.584 ± 0.078

8.533 ± 0.092

10.177 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.07 ± 0.033

7.171 ± 0.087

3.083 ± 0.039

3.966 ± 0.054

2.927 ± 0.04

5.615 ± 0.051

5.634 ± 0.052

6.013 ± 0.055

1.135 ± 0.032

4.213 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski