Amino acid dipepetide frequency for Spiroplasma syrphidicola EA-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.728AlaAla: 3.728 ± 0.147
0.549AlaCys: 0.549 ± 0.041
2.704AlaAsp: 2.704 ± 0.106
3.155AlaGlu: 3.155 ± 0.115
2.851AlaPhe: 2.851 ± 0.106
3.668AlaGly: 3.668 ± 0.125
0.876AlaHis: 0.876 ± 0.053
6.327AlaIle: 6.327 ± 0.175
5.568AlaLys: 5.568 ± 0.142
5.709AlaLeu: 5.709 ± 0.139
1.471AlaMet: 1.471 ± 0.076
3.953AlaAsn: 3.953 ± 0.106
1.663AlaPro: 1.663 ± 0.091
2.329AlaGln: 2.329 ± 0.088
1.951AlaArg: 1.951 ± 0.092
3.161AlaSer: 3.161 ± 0.108
3.518AlaThr: 3.518 ± 0.113
4.085AlaVal: 4.085 ± 0.13
0.609AlaTrp: 0.609 ± 0.043
1.888AlaTyr: 1.888 ± 0.095
0.0AlaXaa: 0.0 ± 0.0
Cys
0.483CysAla: 0.483 ± 0.043
0.087CysCys: 0.087 ± 0.016
0.432CysAsp: 0.432 ± 0.038
0.387CysGlu: 0.387 ± 0.036
0.426CysPhe: 0.426 ± 0.038
0.513CysGly: 0.513 ± 0.04
0.165CysHis: 0.165 ± 0.023
0.441CysIle: 0.441 ± 0.033
0.408CysLys: 0.408 ± 0.039
0.732CysLeu: 0.732 ± 0.049
0.096CysMet: 0.096 ± 0.016
0.363CysAsn: 0.363 ± 0.037
0.255CysPro: 0.255 ± 0.026
0.429CysGln: 0.429 ± 0.039
0.186CysArg: 0.186 ± 0.021
0.432CysSer: 0.432 ± 0.037
0.33CysThr: 0.33 ± 0.029
0.312CysVal: 0.312 ± 0.031
0.09CysTrp: 0.09 ± 0.015
0.348CysTyr: 0.348 ± 0.034
0.0CysXaa: 0.0 ± 0.0
Asp
2.248AspAla: 2.248 ± 0.088
0.342AspCys: 0.342 ± 0.034
2.716AspAsp: 2.716 ± 0.121
3.515AspGlu: 3.515 ± 0.129
3.095AspPhe: 3.095 ± 0.108
2.611AspGly: 2.611 ± 0.108
0.819AspHis: 0.819 ± 0.053
4.634AspIle: 4.634 ± 0.147
4.064AspLys: 4.064 ± 0.144
6.063AspLeu: 6.063 ± 0.145
0.813AspMet: 0.813 ± 0.047
3.851AspAsn: 3.851 ± 0.108
1.615AspPro: 1.615 ± 0.071
2.266AspGln: 2.266 ± 0.075
1.357AspArg: 1.357 ± 0.062
2.764AspSer: 2.764 ± 0.104
1.831AspThr: 1.831 ± 0.07
2.968AspVal: 2.968 ± 0.101
0.609AspTrp: 0.609 ± 0.044
2.71AspTyr: 2.71 ± 0.096
0.0AspXaa: 0.0 ± 0.0
Glu
3.02GluAla: 3.02 ± 0.104
0.291GluCys: 0.291 ± 0.028
2.5GluAsp: 2.5 ± 0.086
4.385GluGlu: 4.385 ± 0.14
2.641GluPhe: 2.641 ± 0.084
2.683GluGly: 2.683 ± 0.09
1.012GluHis: 1.012 ± 0.054
6.555GluIle: 6.555 ± 0.17
6.09GluLys: 6.09 ± 0.159
6.537GluLeu: 6.537 ± 0.156
1.525GluMet: 1.525 ± 0.076
4.172GluAsn: 4.172 ± 0.116
1.525GluPro: 1.525 ± 0.076
3.35GluGln: 3.35 ± 0.126
1.912GluArg: 1.912 ± 0.091
2.194GluSer: 2.194 ± 0.085
2.905GluThr: 2.905 ± 0.112
3.851GluVal: 3.851 ± 0.117
0.591GluTrp: 0.591 ± 0.04
2.488GluTyr: 2.488 ± 0.095
0.0GluXaa: 0.0 ± 0.0
Phe
3.332PheAla: 3.332 ± 0.109
0.459PheCys: 0.459 ± 0.035
2.99PheAsp: 2.99 ± 0.098
2.713PheGlu: 2.713 ± 0.102
2.875PhePhe: 2.875 ± 0.112
2.986PheGly: 2.986 ± 0.11
0.588PheHis: 0.588 ± 0.051
4.841PheIle: 4.841 ± 0.154
3.845PheLys: 3.845 ± 0.117
5.655PheLeu: 5.655 ± 0.156
0.993PheMet: 0.993 ± 0.054
3.881PheAsn: 3.881 ± 0.136
1.336PhePro: 1.336 ± 0.068
1.525PheGln: 1.525 ± 0.071
1.354PheArg: 1.354 ± 0.066
3.584PheSer: 3.584 ± 0.111
2.971PheThr: 2.971 ± 0.105
3.029PheVal: 3.029 ± 0.096
0.69PheTrp: 0.69 ± 0.052
2.107PheTyr: 2.107 ± 0.087
0.0PheXaa: 0.0 ± 0.0
Gly
3.518GlyAla: 3.518 ± 0.124
0.369GlyCys: 0.369 ± 0.035
2.485GlyAsp: 2.485 ± 0.103
3.164GlyGlu: 3.164 ± 0.115
2.944GlyPhe: 2.944 ± 0.114
2.953GlyGly: 2.953 ± 0.128
1.003GlyHis: 1.003 ± 0.059
5.163GlyIle: 5.163 ± 0.141
4.373GlyLys: 4.373 ± 0.126
5.325GlyLeu: 5.325 ± 0.157
1.315GlyMet: 1.315 ± 0.06
2.659GlyAsn: 2.659 ± 0.096
1.48GlyPro: 1.48 ± 0.073
2.188GlyGln: 2.188 ± 0.086
1.606GlyArg: 1.606 ± 0.081
2.86GlySer: 2.86 ± 0.094
3.482GlyThr: 3.482 ± 0.11
3.686GlyVal: 3.686 ± 0.122
0.768GlyTrp: 0.768 ± 0.056
2.641GlyTyr: 2.641 ± 0.097
0.0GlyXaa: 0.0 ± 0.0
His
0.843HisAla: 0.843 ± 0.046
0.135HisCys: 0.135 ± 0.021
0.747HisAsp: 0.747 ± 0.049
0.771HisGlu: 0.771 ± 0.052
0.879HisPhe: 0.879 ± 0.052
0.897HisGly: 0.897 ± 0.048
0.471HisHis: 0.471 ± 0.043
1.129HisIle: 1.129 ± 0.051
0.978HisLys: 0.978 ± 0.059
1.717HisLeu: 1.717 ± 0.074
0.243HisMet: 0.243 ± 0.028
1.069HisAsn: 1.069 ± 0.065
0.678HisPro: 0.678 ± 0.045
0.831HisGln: 0.831 ± 0.051
0.549HisArg: 0.549 ± 0.04
0.852HisSer: 0.852 ± 0.043
0.651HisThr: 0.651 ± 0.045
0.807HisVal: 0.807 ± 0.051
0.189HisTrp: 0.189 ± 0.025
0.801HisTyr: 0.801 ± 0.06
0.0HisXaa: 0.0 ± 0.0
Ile
6.144IleAla: 6.144 ± 0.14
0.828IleCys: 0.828 ± 0.056
4.838IleAsp: 4.838 ± 0.128
4.673IleGlu: 4.673 ± 0.134
5.229IlePhe: 5.229 ± 0.164
5.097IleGly: 5.097 ± 0.157
1.105IleHis: 1.105 ± 0.055
10.307IleIle: 10.307 ± 0.218
8.608IleLys: 8.608 ± 0.191
9.314IleLeu: 9.314 ± 0.198
1.882IleMet: 1.882 ± 0.064
7.672IleAsn: 7.672 ± 0.196
2.845IlePro: 2.845 ± 0.092
2.272IleGln: 2.272 ± 0.098
2.815IleArg: 2.815 ± 0.093
6.081IleSer: 6.081 ± 0.144
5.751IleThr: 5.751 ± 0.135
5.727IleVal: 5.727 ± 0.132
0.915IleTrp: 0.915 ± 0.056
3.734IleTyr: 3.734 ± 0.103
0.0IleXaa: 0.0 ± 0.0
Lys
4.874LysAla: 4.874 ± 0.142
0.351LysCys: 0.351 ± 0.035
4.565LysAsp: 4.565 ± 0.129
6.018LysGlu: 6.018 ± 0.153
3.125LysPhe: 3.125 ± 0.102
3.593LysGly: 3.593 ± 0.115
1.189LysHis: 1.189 ± 0.063
8.668LysIle: 8.668 ± 0.172
8.329LysLys: 8.329 ± 0.202
7.678LysLeu: 7.678 ± 0.157
2.482LysMet: 2.482 ± 0.085
7.033LysAsn: 7.033 ± 0.165
2.53LysPro: 2.53 ± 0.097
3.305LysGln: 3.305 ± 0.103
2.416LysArg: 2.416 ± 0.081
3.821LysSer: 3.821 ± 0.109
5.394LysThr: 5.394 ± 0.114
4.992LysVal: 4.992 ± 0.152
0.939LysTrp: 0.939 ± 0.056
4.028LysTyr: 4.028 ± 0.125
0.0LysXaa: 0.0 ± 0.0
Leu
7.57LeuAla: 7.57 ± 0.178
0.687LeuCys: 0.687 ± 0.046
4.995LeuAsp: 4.995 ± 0.165
6.06LeuGlu: 6.06 ± 0.156
5.202LeuPhe: 5.202 ± 0.148
5.685LeuGly: 5.685 ± 0.149
1.258LeuHis: 1.258 ± 0.055
9.536LeuIle: 9.536 ± 0.189
8.614LeuLys: 8.614 ± 0.165
10.055LeuLeu: 10.055 ± 0.209
1.984LeuMet: 1.984 ± 0.085
6.693LeuAsn: 6.693 ± 0.161
3.218LeuPro: 3.218 ± 0.103
3.533LeuGln: 3.533 ± 0.134
2.68LeuArg: 2.68 ± 0.099
6.393LeuSer: 6.393 ± 0.165
6.684LeuThr: 6.684 ± 0.16
6.567LeuVal: 6.567 ± 0.155
1.018LeuTrp: 1.018 ± 0.054
3.341LeuTyr: 3.341 ± 0.109
0.0LeuXaa: 0.0 ± 0.0
Met
1.753MetAla: 1.753 ± 0.072
0.114MetCys: 0.114 ± 0.018
0.819MetAsp: 0.819 ± 0.052
1.024MetGlu: 1.024 ± 0.05
1.048MetPhe: 1.048 ± 0.057
1.315MetGly: 1.315 ± 0.06
0.291MetHis: 0.291 ± 0.032
1.939MetIle: 1.939 ± 0.08
1.729MetLys: 1.729 ± 0.068
2.26MetLeu: 2.26 ± 0.084
0.558MetMet: 0.558 ± 0.048
1.255MetAsn: 1.255 ± 0.053
0.666MetPro: 0.666 ± 0.046
0.852MetGln: 0.852 ± 0.055
0.612MetArg: 0.612 ± 0.045
1.249MetSer: 1.249 ± 0.06
1.24MetThr: 1.24 ± 0.061
1.165MetVal: 1.165 ± 0.058
0.144MetTrp: 0.144 ± 0.02
0.672MetTyr: 0.672 ± 0.044
0.0MetXaa: 0.0 ± 0.0
Asn
3.107AsnAla: 3.107 ± 0.09
0.465AsnCys: 0.465 ± 0.044
4.22AsnAsp: 4.22 ± 0.145
4.16AsnGlu: 4.16 ± 0.126
3.989AsnPhe: 3.989 ± 0.117
3.347AsnGly: 3.347 ± 0.1
1.186AsnHis: 1.186 ± 0.064
6.126AsnIle: 6.126 ± 0.148
6.15AsnLys: 6.15 ± 0.162
7.72AsnLeu: 7.72 ± 0.188
1.126AsnMet: 1.126 ± 0.06
6.897AsnAsn: 6.897 ± 0.221
2.722AsnPro: 2.722 ± 0.089
3.188AsnGln: 3.188 ± 0.114
2.044AsnArg: 2.044 ± 0.072
4.364AsnSer: 4.364 ± 0.141
2.809AsnThr: 2.809 ± 0.084
3.596AsnVal: 3.596 ± 0.127
0.822AsnTrp: 0.822 ± 0.051
3.674AsnTyr: 3.674 ± 0.123
0.0AsnXaa: 0.0 ± 0.0
Pro
1.864ProAla: 1.864 ± 0.079
0.165ProCys: 0.165 ± 0.023
1.519ProAsp: 1.519 ± 0.069
2.359ProGlu: 2.359 ± 0.109
1.675ProPhe: 1.675 ± 0.07
1.984ProGly: 1.984 ± 0.094
0.48ProHis: 0.48 ± 0.038
2.758ProIle: 2.758 ± 0.097
2.293ProLys: 2.293 ± 0.092
2.878ProLeu: 2.878 ± 0.099
0.576ProMet: 0.576 ± 0.044
2.071ProAsn: 2.071 ± 0.091
0.825ProPro: 0.825 ± 0.054
1.144ProGln: 1.144 ± 0.068
0.759ProArg: 0.759 ± 0.042
1.735ProSer: 1.735 ± 0.075
2.101ProThr: 2.101 ± 0.086
2.167ProVal: 2.167 ± 0.093
0.369ProTrp: 0.369 ± 0.034
1.102ProTyr: 1.102 ± 0.063
0.0ProXaa: 0.0 ± 0.0
Gln
2.359GlnAla: 2.359 ± 0.09
0.177GlnCys: 0.177 ± 0.024
1.864GlnAsp: 1.864 ± 0.074
2.959GlnGlu: 2.959 ± 0.093
1.801GlnPhe: 1.801 ± 0.069
1.828GlnGly: 1.828 ± 0.081
0.618GlnHis: 0.618 ± 0.04
3.512GlnIle: 3.512 ± 0.11
3.764GlnLys: 3.764 ± 0.114
4.16GlnLeu: 4.16 ± 0.129
0.813GlnMet: 0.813 ± 0.045
2.845GlnAsn: 2.845 ± 0.102
1.27GlnPro: 1.27 ± 0.067
2.233GlnGln: 2.233 ± 0.108
1.246GlnArg: 1.246 ± 0.062
1.654GlnSer: 1.654 ± 0.071
2.416GlnThr: 2.416 ± 0.082
1.975GlnVal: 1.975 ± 0.071
0.471GlnTrp: 0.471 ± 0.04
1.699GlnTyr: 1.699 ± 0.073
0.0GlnXaa: 0.0 ± 0.0
Arg
1.636ArgAla: 1.636 ± 0.067
0.21ArgCys: 0.21 ± 0.026
1.483ArgAsp: 1.483 ± 0.061
2.071ArgGlu: 2.071 ± 0.093
1.321ArgPhe: 1.321 ± 0.058
1.501ArgGly: 1.501 ± 0.078
0.561ArgHis: 0.561 ± 0.038
2.758ArgIle: 2.758 ± 0.09
2.59ArgLys: 2.59 ± 0.095
2.812ArgLeu: 2.812 ± 0.117
0.72ArgMet: 0.72 ± 0.046
1.828ArgAsn: 1.828 ± 0.09
0.942ArgPro: 0.942 ± 0.061
1.39ArgGln: 1.39 ± 0.068
1.204ArgArg: 1.204 ± 0.072
1.486ArgSer: 1.486 ± 0.061
1.516ArgThr: 1.516 ± 0.068
1.588ArgVal: 1.588 ± 0.075
0.366ArgTrp: 0.366 ± 0.033
1.288ArgTyr: 1.288 ± 0.057
0.0ArgXaa: 0.0 ± 0.0
Ser
3.194SerAla: 3.194 ± 0.109
0.414SerCys: 0.414 ± 0.035
2.893SerAsp: 2.893 ± 0.1
3.479SerGlu: 3.479 ± 0.106
3.359SerPhe: 3.359 ± 0.12
3.305SerGly: 3.305 ± 0.103
0.903SerHis: 0.903 ± 0.052
4.718SerIle: 4.718 ± 0.137
4.418SerLys: 4.418 ± 0.11
5.964SerLeu: 5.964 ± 0.149
1.021SerMet: 1.021 ± 0.055
3.806SerAsn: 3.806 ± 0.147
1.843SerPro: 1.843 ± 0.073
2.209SerGln: 2.209 ± 0.094
1.633SerArg: 1.633 ± 0.081
3.386SerSer: 3.386 ± 0.123
2.99SerThr: 2.99 ± 0.104
3.128SerVal: 3.128 ± 0.094
0.774SerTrp: 0.774 ± 0.048
2.716SerTyr: 2.716 ± 0.094
0.0SerXaa: 0.0 ± 0.0
Thr
3.509ThrAla: 3.509 ± 0.113
0.285ThrCys: 0.285 ± 0.031
2.677ThrAsp: 2.677 ± 0.097
2.857ThrGlu: 2.857 ± 0.09
2.86ThrPhe: 2.86 ± 0.094
3.536ThrGly: 3.536 ± 0.112
0.693ThrHis: 0.693 ± 0.046
6.324ThrIle: 6.324 ± 0.166
4.763ThrLys: 4.763 ± 0.124
5.259ThrLeu: 5.259 ± 0.123
0.909ThrMet: 0.909 ± 0.05
3.89ThrAsn: 3.89 ± 0.121
2.188ThrPro: 2.188 ± 0.09
1.417ThrGln: 1.417 ± 0.077
1.453ThrArg: 1.453 ± 0.068
3.371ThrSer: 3.371 ± 0.117
3.947ThrThr: 3.947 ± 0.123
3.77ThrVal: 3.77 ± 0.124
0.63ThrTrp: 0.63 ± 0.05
1.999ThrTyr: 1.999 ± 0.083
0.0ThrXaa: 0.0 ± 0.0
Val
3.803ValAla: 3.803 ± 0.113
0.534ValCys: 0.534 ± 0.046
3.149ValAsp: 3.149 ± 0.101
3.686ValGlu: 3.686 ± 0.113
2.884ValPhe: 2.884 ± 0.092
3.728ValGly: 3.728 ± 0.115
0.729ValHis: 0.729 ± 0.056
6.126ValIle: 6.126 ± 0.136
5.322ValLys: 5.322 ± 0.155
5.925ValLeu: 5.925 ± 0.13
1.237ValMet: 1.237 ± 0.065
3.992ValAsn: 3.992 ± 0.118
1.816ValPro: 1.816 ± 0.072
1.726ValGln: 1.726 ± 0.071
1.678ValArg: 1.678 ± 0.066
3.56ValSer: 3.56 ± 0.113
3.503ValThr: 3.503 ± 0.126
4.031ValVal: 4.031 ± 0.109
0.567ValTrp: 0.567 ± 0.044
2.137ValTyr: 2.137 ± 0.085
0.0ValXaa: 0.0 ± 0.0
Trp
0.63TrpAla: 0.63 ± 0.05
0.069TrpCys: 0.069 ± 0.014
0.564TrpAsp: 0.564 ± 0.041
0.642TrpGlu: 0.642 ± 0.043
0.783TrpPhe: 0.783 ± 0.049
0.558TrpGly: 0.558 ± 0.048
0.192TrpHis: 0.192 ± 0.028
1.189TrpIle: 1.189 ± 0.063
0.87TrpLys: 0.87 ± 0.052
1.069TrpLeu: 1.069 ± 0.053
0.252TrpMet: 0.252 ± 0.03
0.81TrpAsn: 0.81 ± 0.051
0.282TrpPro: 0.282 ± 0.03
0.438TrpGln: 0.438 ± 0.037
0.309TrpArg: 0.309 ± 0.032
0.663TrpSer: 0.663 ± 0.049
0.633TrpThr: 0.633 ± 0.038
0.621TrpVal: 0.621 ± 0.044
0.216TrpTrp: 0.216 ± 0.029
0.558TrpTyr: 0.558 ± 0.047
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.149TyrAla: 2.149 ± 0.083
0.384TyrCys: 0.384 ± 0.033
2.731TyrAsp: 2.731 ± 0.107
2.149TyrGlu: 2.149 ± 0.078
2.767TyrPhe: 2.767 ± 0.091
2.194TyrGly: 2.194 ± 0.082
1.102TyrHis: 1.102 ± 0.064
2.785TyrIle: 2.785 ± 0.094
2.551TyrLys: 2.551 ± 0.093
4.94TyrLeu: 4.94 ± 0.151
0.684TyrMet: 0.684 ± 0.053
2.971TyrAsn: 2.971 ± 0.104
1.126TyrPro: 1.126 ± 0.059
3.041TyrGln: 3.041 ± 0.12
1.51TyrArg: 1.51 ± 0.073
2.542TyrSer: 2.542 ± 0.098
1.666TyrThr: 1.666 ± 0.077
2.068TyrVal: 2.068 ± 0.077
0.57TyrTrp: 0.57 ± 0.043
2.194TyrTyr: 2.194 ± 0.088
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1006 proteins (333167 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski