Winogradskyella sediminis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Winogradskyella

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3162 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H1X232|A0A1H1X232_9FLAO Helix-turn-helix Psq domain OS=Winogradskyella sediminis OX=1382466 GN=SAMN04489797_3073 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 10.37RR3 pKa = 11.84LLSFSLCFGILLFWSSCRR21 pKa = 11.84NDD23 pKa = 3.92FEE25 pKa = 4.5FTPSKK30 pKa = 11.18GNLEE34 pKa = 4.2FAQDD38 pKa = 4.11TIYY41 pKa = 11.18LDD43 pKa = 3.74TVFTNIGSSTYY54 pKa = 9.91NLKK57 pKa = 10.64VYY59 pKa = 10.31NRR61 pKa = 11.84SDD63 pKa = 3.82DD64 pKa = 5.08DD65 pKa = 3.74IVIPTIQFEE74 pKa = 4.32NGVNSLYY81 pKa = 10.96RR82 pKa = 11.84MNIDD86 pKa = 3.31GNTGLEE92 pKa = 4.1DD93 pKa = 3.57AQEE96 pKa = 4.27GKK98 pKa = 10.61FFEE101 pKa = 5.1DD102 pKa = 4.23VEE104 pKa = 4.47LLANDD109 pKa = 3.69SLFIFIEE116 pKa = 4.18TTIDD120 pKa = 3.13ISTLGALEE128 pKa = 4.04NQFLYY133 pKa = 10.04TDD135 pKa = 4.99KK136 pKa = 11.12ILFDD140 pKa = 4.29SGNNQQDD147 pKa = 3.12IDD149 pKa = 4.18VVTLVKK155 pKa = 10.54DD156 pKa = 3.42AVFIYY161 pKa = 9.81PSKK164 pKa = 11.13DD165 pKa = 3.06DD166 pKa = 3.29TTGIVEE172 pKa = 4.3TLSFDD177 pKa = 3.28VDD179 pKa = 3.57GDD181 pKa = 3.93GTPDD185 pKa = 3.16EE186 pKa = 4.53TTLEE190 pKa = 4.27GRR192 pKa = 11.84FLTDD196 pKa = 4.93DD197 pKa = 3.62EE198 pKa = 4.75LTFTNEE204 pKa = 3.56KK205 pKa = 10.09PYY207 pKa = 10.86VIYY210 pKa = 10.07GYY212 pKa = 11.03AGVGSGKK219 pKa = 8.08TLTMEE224 pKa = 4.56AGARR228 pKa = 11.84VHH230 pKa = 6.27FHH232 pKa = 7.05ADD234 pKa = 2.64SGIIVTDD241 pKa = 3.63NASLQINGALSTDD254 pKa = 3.38QEE256 pKa = 4.41TLEE259 pKa = 4.44NEE261 pKa = 4.52VILEE265 pKa = 3.94GDD267 pKa = 3.35RR268 pKa = 11.84LEE270 pKa = 4.65PLYY273 pKa = 10.94EE274 pKa = 5.22DD275 pKa = 5.6IPGQWGTIWLYY286 pKa = 11.12NGSEE290 pKa = 4.23NNTINYY296 pKa = 7.49ATIKK300 pKa = 10.29NATIGVLSEE309 pKa = 4.38GNQNAPTDD317 pKa = 3.74KK318 pKa = 10.54LTITNTQIYY327 pKa = 8.86NVSSFGILGRR337 pKa = 11.84ATSIRR342 pKa = 11.84AEE344 pKa = 3.63NLVINNAGQSSFAGTYY360 pKa = 8.12GGKK363 pKa = 10.68YY364 pKa = 9.81NVTHH368 pKa = 5.44STIANYY374 pKa = 9.01WNSSFRR380 pKa = 11.84EE381 pKa = 4.1YY382 pKa = 10.3PALLINDD389 pKa = 4.62YY390 pKa = 10.99ILDD393 pKa = 3.91EE394 pKa = 4.67NNTIFTNDD402 pKa = 2.86LTEE405 pKa = 5.08ANFNNCIIYY414 pKa = 10.83GNDD417 pKa = 3.09NPEE420 pKa = 4.42FILEE424 pKa = 4.15NEE426 pKa = 4.02GDD428 pKa = 3.75VFNYY432 pKa = 10.37KK433 pKa = 8.62FTNCLLRR440 pKa = 11.84FNDD443 pKa = 4.72NNLQGNGNYY452 pKa = 10.27NFDD455 pKa = 3.6DD456 pKa = 3.57TAFYY460 pKa = 10.24IDD462 pKa = 4.1NVFNEE467 pKa = 4.38NPDD470 pKa = 3.45FEE472 pKa = 5.82DD473 pKa = 4.68PYY475 pKa = 10.87EE476 pKa = 4.53SLMKK480 pKa = 10.23IGEE483 pKa = 4.32DD484 pKa = 3.54SSAKK488 pKa = 10.43GIGSATYY495 pKa = 11.06SNLVLNDD502 pKa = 3.17ILGASRR508 pKa = 11.84IDD510 pKa = 3.47SGSFDD515 pKa = 3.07AGAYY519 pKa = 9.5NFSTFEE525 pKa = 4.1DD526 pKa = 3.59

Molecular weight: 58.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H1N9V9|A0A1H1N9V9_9FLAO Phosphoribosylformylglycinamidine synthase OS=Winogradskyella sediminis OX=1382466 GN=purL PE=3 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.06RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.18RR22 pKa = 11.84MASVNGRR29 pKa = 11.84KK30 pKa = 9.21VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 8.0KK42 pKa = 10.66LSVSTEE48 pKa = 3.92TRR50 pKa = 11.84HH51 pKa = 6.44KK52 pKa = 10.57KK53 pKa = 9.81

Molecular weight: 6.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3162

0

3162

1098209

29

3263

347.3

39.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.44 ± 0.037

0.754 ± 0.016

5.875 ± 0.039

6.472 ± 0.042

5.14 ± 0.037

6.158 ± 0.041

1.835 ± 0.021

8.061 ± 0.041

7.417 ± 0.068

9.253 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.117 ± 0.021

6.338 ± 0.052

3.279 ± 0.028

3.333 ± 0.022

3.219 ± 0.031

6.667 ± 0.039

6.25 ± 0.061

6.243 ± 0.032

0.985 ± 0.015

4.166 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski