Klebsiella phage vB_KpnS_IME279

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Sortsnevirus; Klebsiella virus IME279

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291LBL1|A0A291LBL1_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnS_IME279 OX=2041211 PE=4 SV=1
MM1 pKa = 6.61TTLAQTLADD10 pKa = 3.23IQAEE14 pKa = 4.2IEE16 pKa = 4.1RR17 pKa = 11.84QKK19 pKa = 11.62ASGNLSPLVAGIIANEE35 pKa = 3.72AAEE38 pKa = 4.05RR39 pKa = 11.84AEE41 pKa = 4.34VDD43 pKa = 4.83DD44 pKa = 4.52LTPDD48 pKa = 2.96EE49 pKa = 4.63WDD51 pKa = 3.88LVDD54 pKa = 6.71DD55 pKa = 4.57EE56 pKa = 4.74AQLRR60 pKa = 11.84ALPGYY65 pKa = 10.69YY66 pKa = 10.15DD67 pKa = 3.61GFF69 pKa = 4.68

Molecular weight: 7.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A291LBA5|A0A291LBA5_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnS_IME279 OX=2041211 PE=4 SV=1
MM1 pKa = 7.51IPADD5 pKa = 3.55IQRR8 pKa = 11.84KK9 pKa = 7.9AKK11 pKa = 10.21KK12 pKa = 9.68VLQRR16 pKa = 11.84HH17 pKa = 5.55LAGEE21 pKa = 4.29IKK23 pKa = 9.88PRR25 pKa = 11.84KK26 pKa = 9.06LVGGLGLALEE36 pKa = 4.42VGYY39 pKa = 10.27RR40 pKa = 11.84WRR42 pKa = 11.84LLSRR46 pKa = 11.84DD47 pKa = 3.79KK48 pKa = 11.45GQTWDD53 pKa = 3.52LMSHH57 pKa = 5.82EE58 pKa = 5.37KK59 pKa = 10.63YY60 pKa = 10.83NKK62 pKa = 8.27LTRR65 pKa = 11.84GKK67 pKa = 9.82KK68 pKa = 7.52PPKK71 pKa = 9.42MRR73 pKa = 4.84

Molecular weight: 8.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

13193

57

1047

223.6

24.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.968 ± 0.639

1.054 ± 0.178

6.663 ± 0.206

6.253 ± 0.403

3.032 ± 0.173

8.224 ± 0.48

1.865 ± 0.223

5.063 ± 0.297

5.435 ± 0.266

7.762 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.926 ± 0.172

4.267 ± 0.277

4.79 ± 0.234

4.927 ± 0.431

6.094 ± 0.326

4.624 ± 0.216

5.473 ± 0.325

5.897 ± 0.311

1.675 ± 0.129

3.009 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski