Mycobacterium phage Corndog (Mycobacteriophage Corndog)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Corndogvirus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 122 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q856Q5|Q856Q5_BPMCO Uncharacterized protein OS=Mycobacterium phage Corndog OX=205875 GN=39 PE=4 SV=1
MM1 pKa = 7.26KK2 pKa = 10.16VWFQGRR8 pKa = 11.84YY9 pKa = 8.0IDD11 pKa = 3.66VAVTVVEE18 pKa = 4.4GDD20 pKa = 3.5DD21 pKa = 3.77GVPYY25 pKa = 10.57VYY27 pKa = 10.93VDD29 pKa = 3.77VPDD32 pKa = 4.8GPDD35 pKa = 3.19GGGEE39 pKa = 4.14PLPAAAA45 pKa = 5.69

Molecular weight: 4.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q856K7|KU_BPMCO Protein Ku OS=Mycobacterium phage Corndog OX=205875 GN=87 PE=1 SV=1
MM1 pKa = 7.19RR2 pKa = 11.84TCLVCGTTIKK12 pKa = 10.67EE13 pKa = 4.1PIGRR17 pKa = 11.84GRR19 pKa = 11.84PTEE22 pKa = 4.17LCSDD26 pKa = 3.89KK27 pKa = 10.95CRR29 pKa = 11.84AEE31 pKa = 4.59RR32 pKa = 11.84KK33 pKa = 6.91MQQRR37 pKa = 11.84SEE39 pKa = 3.9SRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84AIARR47 pKa = 11.84GIPAHH52 pKa = 6.05LHH54 pKa = 4.65GTSTGSTYY62 pKa = 10.45YY63 pKa = 10.32RR64 pKa = 11.84CSCDD68 pKa = 3.38KK69 pKa = 10.66CRR71 pKa = 11.84EE72 pKa = 3.71WSRR75 pKa = 11.84TYY77 pKa = 9.84QQARR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84GQLATEE89 pKa = 4.51GRR91 pKa = 3.9

Molecular weight: 10.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

122

0

122

21973

33

1461

180.1

19.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.545 ± 0.322

1.292 ± 0.157

6.208 ± 0.187

6.604 ± 0.283

2.945 ± 0.143

8.715 ± 0.349

1.934 ± 0.149

4.474 ± 0.186

3.509 ± 0.181

7.814 ± 0.198

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.562 ± 0.117

3.086 ± 0.167

6.189 ± 0.24

3.309 ± 0.173

7.163 ± 0.301

5.384 ± 0.183

6.076 ± 0.182

7.518 ± 0.244

2.194 ± 0.124

2.48 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski