Maricaulis maris (strain MCS10)
Average proteome isoelectric point is 5.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3060 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q0ANE1|SURE_MARMM 5'-nucleotidase SurE OS=Maricaulis maris (strain MCS10) OX=394221 GN=surE PE=3 SV=1
MM1 pKa = 7.27 KK2 pKa = 9.73 TFKK5 pKa = 10.82 YY6 pKa = 10.82 LMIGASALVLTACGNSDD23 pKa = 3.47 TSRR26 pKa = 11.84 DD27 pKa = 3.44 LAEE30 pKa = 4.94 ANVDD34 pKa = 3.66 YY35 pKa = 10.83 QATLMEE41 pKa = 4.31 QLIDD45 pKa = 3.89 AQPGDD50 pKa = 4.32 VIEE53 pKa = 4.9 IPAGVYY59 pKa = 10.55 SFDD62 pKa = 4.23 RR63 pKa = 11.84 SLSLNIDD70 pKa = 3.36 GVTIRR75 pKa = 11.84 GAGMDD80 pKa = 3.59 EE81 pKa = 4.73 TILSFQDD88 pKa = 3.26 QIAGAEE94 pKa = 4.14 GLIVTANDD102 pKa = 3.28 FTLEE106 pKa = 3.89 NLAIEE111 pKa = 4.3 DD112 pKa = 4.42 TIGDD116 pKa = 3.93 GLKK119 pKa = 10.96 VNGGEE124 pKa = 4.08 NIIIRR129 pKa = 11.84 GVRR132 pKa = 11.84 VEE134 pKa = 4.05 WTNGYY139 pKa = 8.55 ATEE142 pKa = 4.13 NGAYY146 pKa = 10.01 GIYY149 pKa = 9.89 PVQTTNVLVEE159 pKa = 4.06 DD160 pKa = 4.52 TVAIGASDD168 pKa = 3.18 AGIYY172 pKa = 9.7 VGQSRR177 pKa = 11.84 NVIVRR182 pKa = 11.84 NSRR185 pKa = 11.84 AEE187 pKa = 3.9 YY188 pKa = 9.36 NVAGIEE194 pKa = 3.97 IEE196 pKa = 4.11 NCIGADD202 pKa = 3.53 VYY204 pKa = 11.79 GNTATNNTGGILVFNMPNLPQPGYY228 pKa = 8.38 HH229 pKa = 5.26 TRR231 pKa = 11.84 VYY233 pKa = 11.3 DD234 pKa = 3.91 NDD236 pKa = 2.89 IFANNTEE243 pKa = 4.32 NFGHH247 pKa = 7.3 AGTPVASVPAGSGVVINSNDD267 pKa = 3.22 QVEE270 pKa = 4.37 IFNNRR275 pKa = 11.84 ISDD278 pKa = 3.73 NDD280 pKa = 3.72 TANIIISSLHH290 pKa = 4.61 STGYY294 pKa = 10.35 SDD296 pKa = 5.63 YY297 pKa = 11.41 SVQTDD302 pKa = 4.0 FDD304 pKa = 5.06 PYY306 pKa = 10.66 PEE308 pKa = 5.15 SIWIHH313 pKa = 6.75 DD314 pKa = 3.73 NTYY317 pKa = 10.88 SGGGTSPDD325 pKa = 3.77 GLDD328 pKa = 3.27 LTALKK333 pKa = 10.37 IAMFGLNGALPDD345 pKa = 3.59 VLFDD349 pKa = 5.13 GFVDD353 pKa = 4.2 DD354 pKa = 5.91 AKK356 pKa = 11.12 LVDD359 pKa = 4.12 GALPDD364 pKa = 3.63 ALRR367 pKa = 11.84 ICVSDD372 pKa = 3.77 PVDD375 pKa = 3.42 VLNADD380 pKa = 3.85 GPNGYY385 pKa = 10.34 ASPSVDD391 pKa = 3.14 TEE393 pKa = 4.23 DD394 pKa = 4.41 FRR396 pKa = 11.84 CSLDD400 pKa = 3.37 SLSAVEE406 pKa = 5.51 LAFADD411 pKa = 3.72
Molecular weight: 43.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.688
IPC2_protein 3.745
IPC_protein 3.783
Toseland 3.554
ProMoST 3.923
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.452
Solomon 3.77
Lehninger 3.732
Nozaki 3.884
DTASelect 4.151
Thurlkill 3.605
EMBOSS 3.732
Sillero 3.897
Patrickios 1.036
IPC_peptide 3.77
IPC2_peptide 3.872
IPC2.peptide.svr19 3.807
Protein with the highest isoelectric point:
>tr|Q0ASZ6|Q0ASZ6_MARMM Proline racemase OS=Maricaulis maris (strain MCS10) OX=394221 GN=Mmar10_0298 PE=3 SV=1
MM1 pKa = 7.38 TNVGHH6 pKa = 7.52 KK7 pKa = 10.2 KK8 pKa = 10.07 RR9 pKa = 11.84 IAPRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 PRR17 pKa = 11.84 HH18 pKa = 5.26 PRR20 pKa = 11.84 PMTRR24 pKa = 11.84 PSPLQNRR31 pKa = 11.84 VTPTGEE37 pKa = 3.69 ICAHH41 pKa = 6.9 AARR44 pKa = 11.84 GTLMGNRR51 pKa = 11.84 GILHH55 pKa = 7.33 DD56 pKa = 4.45 EE57 pKa = 4.08 RR58 pKa = 11.84 QQLGTARR65 pKa = 11.84 WKK67 pKa = 8.72 HH68 pKa = 4.65 QAWVTCTLAFKK79 pKa = 10.62 ARR81 pKa = 11.84 RR82 pKa = 11.84 RR83 pKa = 11.84 DD84 pKa = 3.35 LMKK87 pKa = 10.31 PGNYY91 pKa = 8.88 TEE93 pKa = 6.0 LFFTDD98 pKa = 3.44 EE99 pKa = 4.24 AVALAAGHH107 pKa = 6.5 RR108 pKa = 11.84 PCGEE112 pKa = 3.92 CRR114 pKa = 11.84 RR115 pKa = 11.84 EE116 pKa = 3.86 DD117 pKa = 3.6 FNRR120 pKa = 11.84 FRR122 pKa = 11.84 DD123 pKa = 4.06 AFAKK127 pKa = 10.07 AHH129 pKa = 7.12 PDD131 pKa = 3.44 LPHH134 pKa = 7.27 RR135 pKa = 11.84 APDD138 pKa = 3.54 MDD140 pKa = 3.75 RR141 pKa = 11.84 LMHH144 pKa = 6.67 KK145 pKa = 10.28 ARR147 pKa = 11.84 VVSRR151 pKa = 11.84 SRR153 pKa = 11.84 AQVTYY158 pKa = 10.64 EE159 pKa = 4.33 AMLSDD164 pKa = 3.84 LPEE167 pKa = 4.12 GVFFRR172 pKa = 11.84 VAPAGGPLVRR182 pKa = 11.84 WQGKK186 pKa = 7.23 VLAWSFEE193 pKa = 4.29 GYY195 pKa = 9.8 SAGPDD200 pKa = 3.33 ALPEE204 pKa = 4.01 RR205 pKa = 11.84 VEE207 pKa = 4.14 VLTPEE212 pKa = 4.24 PTVKK216 pKa = 10.59 AIAAGYY222 pKa = 10.1 VPVCIATGG230 pKa = 3.22
Molecular weight: 25.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 9.414
IPC_protein 10.043
Toseland 10.482
ProMoST 10.16
Dawson 10.584
Bjellqvist 10.306
Wikipedia 10.789
Rodwell 10.76
Grimsley 10.643
Solomon 10.687
Lehninger 10.657
Nozaki 10.496
DTASelect 10.292
Thurlkill 10.482
EMBOSS 10.877
Sillero 10.526
Patrickios 10.467
IPC_peptide 10.701
IPC2_peptide 9.458
IPC2.peptide.svr19 8.566
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3060
0
3060
1006057
41
4368
328.8
35.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.701 ± 0.059
0.787 ± 0.015
6.623 ± 0.04
5.988 ± 0.047
3.734 ± 0.033
8.814 ± 0.097
1.957 ± 0.025
5.029 ± 0.03
2.49 ± 0.046
9.842 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.497 ± 0.025
2.595 ± 0.038
4.938 ± 0.035
3.061 ± 0.023
7.12 ± 0.055
5.65 ± 0.046
5.42 ± 0.046
7.177 ± 0.035
1.431 ± 0.022
2.147 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here