Thiorhodovibrio sp. 970
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4691 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H8Z5M8|H8Z5M8_9GAMM MoxR-like ATPase OS=Thiorhodovibrio sp. 970 OX=631362 GN=Thi970DRAFT_04252 PE=4 SV=1
MM1 pKa = 7.58 TMTTDD6 pKa = 3.56 LTGDD10 pKa = 3.47 APLVFTNSAAAKK22 pKa = 9.63 VADD25 pKa = 4.72 LIKK28 pKa = 10.92 EE29 pKa = 4.13 EE30 pKa = 4.41 GNEE33 pKa = 3.84 EE34 pKa = 3.89 LMLRR38 pKa = 11.84 VYY40 pKa = 10.25 IQGGGCSGFQYY51 pKa = 10.95 GFTFDD56 pKa = 4.22 EE57 pKa = 4.46 QVQDD61 pKa = 3.39 GDD63 pKa = 4.26 TEE65 pKa = 4.62 VITDD69 pKa = 4.05 GVKK72 pKa = 10.77 LLIDD76 pKa = 4.19 PMSMQYY82 pKa = 9.18 LTGAEE87 pKa = 3.72 IDD89 pKa = 3.84 YY90 pKa = 11.45 SEE92 pKa = 4.69 GLQGAQFVIRR102 pKa = 11.84 NPNATTTCGCGSSFDD117 pKa = 3.7 VV118 pKa = 4.17
Molecular weight: 12.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.732
IPC_protein 3.681
Toseland 3.478
ProMoST 3.859
Dawson 3.668
Bjellqvist 3.834
Wikipedia 3.617
Rodwell 3.516
Grimsley 3.389
Solomon 3.643
Lehninger 3.605
Nozaki 3.808
DTASelect 3.999
Thurlkill 3.541
EMBOSS 3.63
Sillero 3.795
Patrickios 0.769
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.732
Protein with the highest isoelectric point:
>tr|H8Z2L5|H8Z2L5_9GAMM FimV N-terminal domain protein OS=Thiorhodovibrio sp. 970 OX=631362 GN=Thi970DRAFT_02987 PE=4 SV=1
MM1 pKa = 7.49 PHH3 pKa = 6.76 LEE5 pKa = 3.64 NWLYY9 pKa = 11.42 GLAFFLSIAVFLYY22 pKa = 10.78 SVFDD26 pKa = 3.22 LTRR29 pKa = 11.84 RR30 pKa = 11.84 LLARR34 pKa = 11.84 FALRR38 pKa = 11.84 VCRR41 pKa = 11.84 MRR43 pKa = 11.84 EE44 pKa = 3.69 ARR46 pKa = 11.84 EE47 pKa = 3.85 GGFICEE53 pKa = 5.25 LKK55 pKa = 10.68 RR56 pKa = 11.84 KK57 pKa = 8.98 LQGFASRR64 pKa = 11.84 SLVSRR69 pKa = 11.84 VLLLVGSFGAALFIIIEE86 pKa = 4.21 HH87 pKa = 5.37 SAKK90 pKa = 10.61 LL91 pKa = 3.54
Molecular weight: 10.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.604
IPC_protein 10.54
Toseland 10.613
ProMoST 10.379
Dawson 10.73
Bjellqvist 10.482
Wikipedia 10.965
Rodwell 10.833
Grimsley 10.789
Solomon 10.877
Lehninger 10.833
Nozaki 10.628
DTASelect 10.467
Thurlkill 10.628
EMBOSS 11.038
Sillero 10.672
Patrickios 10.643
IPC_peptide 10.877
IPC2_peptide 9.75
IPC2.peptide.svr19 8.468
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4691
0
4691
1526990
23
2941
325.5
35.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.419 ± 0.047
1.048 ± 0.015
5.926 ± 0.025
6.228 ± 0.034
3.541 ± 0.022
7.591 ± 0.033
2.311 ± 0.021
4.865 ± 0.028
2.999 ± 0.029
11.567 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.146 ± 0.018
2.685 ± 0.022
5.333 ± 0.03
4.307 ± 0.029
7.505 ± 0.042
5.554 ± 0.029
4.869 ± 0.027
6.382 ± 0.033
1.435 ± 0.015
2.289 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here