Thiorhodovibrio sp. 970

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; Thiorhodovibrio; unclassified Thiorhodovibrio

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4691 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|H8Z5M8|H8Z5M8_9GAMM MoxR-like ATPase OS=Thiorhodovibrio sp. 970 OX=631362 GN=Thi970DRAFT_04252 PE=4 SV=1
MM1 pKa = 7.58TMTTDD6 pKa = 3.56LTGDD10 pKa = 3.47APLVFTNSAAAKK22 pKa = 9.63VADD25 pKa = 4.72LIKK28 pKa = 10.92EE29 pKa = 4.13EE30 pKa = 4.41GNEE33 pKa = 3.84EE34 pKa = 3.89LMLRR38 pKa = 11.84VYY40 pKa = 10.25IQGGGCSGFQYY51 pKa = 10.95GFTFDD56 pKa = 4.22EE57 pKa = 4.46QVQDD61 pKa = 3.39GDD63 pKa = 4.26TEE65 pKa = 4.62VITDD69 pKa = 4.05GVKK72 pKa = 10.77LLIDD76 pKa = 4.19PMSMQYY82 pKa = 9.18LTGAEE87 pKa = 3.72IDD89 pKa = 3.84YY90 pKa = 11.45SEE92 pKa = 4.69GLQGAQFVIRR102 pKa = 11.84NPNATTTCGCGSSFDD117 pKa = 3.7VV118 pKa = 4.17

Molecular weight:
12.64 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|H8Z2L5|H8Z2L5_9GAMM FimV N-terminal domain protein OS=Thiorhodovibrio sp. 970 OX=631362 GN=Thi970DRAFT_02987 PE=4 SV=1
MM1 pKa = 7.49PHH3 pKa = 6.76LEE5 pKa = 3.64NWLYY9 pKa = 11.42GLAFFLSIAVFLYY22 pKa = 10.78SVFDD26 pKa = 3.22LTRR29 pKa = 11.84RR30 pKa = 11.84LLARR34 pKa = 11.84FALRR38 pKa = 11.84VCRR41 pKa = 11.84MRR43 pKa = 11.84EE44 pKa = 3.69ARR46 pKa = 11.84EE47 pKa = 3.85GGFICEE53 pKa = 5.25LKK55 pKa = 10.68RR56 pKa = 11.84KK57 pKa = 8.98LQGFASRR64 pKa = 11.84SLVSRR69 pKa = 11.84VLLLVGSFGAALFIIIEE86 pKa = 4.21HH87 pKa = 5.37SAKK90 pKa = 10.61LL91 pKa = 3.54

Molecular weight:
10.42 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4691

0

4691

1526990

23

2941

325.5

35.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.419 ± 0.047

1.048 ± 0.015

5.926 ± 0.025

6.228 ± 0.034

3.541 ± 0.022

7.591 ± 0.033

2.311 ± 0.021

4.865 ± 0.028

2.999 ± 0.029

11.567 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.146 ± 0.018

2.685 ± 0.022

5.333 ± 0.03

4.307 ± 0.029

7.505 ± 0.042

5.554 ± 0.029

4.869 ± 0.027

6.382 ± 0.033

1.435 ± 0.015

2.289 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski