Roseobacter sp. SK209-2-6

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseobacter; unclassified Roseobacter

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4497 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4EZ96|A4EZ96_9RHOB 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase OS=Roseobacter sp. SK209-2-6 OX=388739 GN=ispE PE=3 SV=1
MM1 pKa = 8.18PITLQVIYY9 pKa = 9.12PASADD14 pKa = 3.2ASFDD18 pKa = 3.27YY19 pKa = 10.72DD20 pKa = 4.0YY21 pKa = 11.49YY22 pKa = 11.33LDD24 pKa = 3.57SHH26 pKa = 6.93LPLVQEE32 pKa = 4.49HH33 pKa = 5.75WGEE36 pKa = 4.0MIEE39 pKa = 4.21GVEE42 pKa = 4.25VAKK45 pKa = 10.91GLASGPDD52 pKa = 3.52VPPAYY57 pKa = 10.36LVITTITFPDD67 pKa = 4.03MEE69 pKa = 4.62TLDD72 pKa = 3.78KK73 pKa = 11.65AMGEE77 pKa = 4.11AGGVIVDD84 pKa = 4.58DD85 pKa = 4.19VPNFTNVRR93 pKa = 11.84PQILIGEE100 pKa = 5.14LIHH103 pKa = 7.23GG104 pKa = 4.45

Molecular weight: 11.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4F049|A4F049_9RHOB Alkylhydroperoxidase AhpD family core domain protein OS=Roseobacter sp. SK209-2-6 OX=388739 GN=RSK20926_10239 PE=4 SV=1
MM1 pKa = 7.59AKK3 pKa = 9.96SRR5 pKa = 11.84LQIMPTRR12 pKa = 11.84QVKK15 pKa = 8.77PQISRR20 pKa = 11.84KK21 pKa = 6.47SQRR24 pKa = 11.84KK25 pKa = 8.02GASNNPSSPLGFDD38 pKa = 2.76AGLRR42 pKa = 11.84FAAFLGFGAALGLAAGLAFGLPPKK66 pKa = 10.23RR67 pKa = 11.84PRR69 pKa = 11.84KK70 pKa = 8.89PRR72 pKa = 11.84LFALVVRR79 pKa = 11.84FLAPGLPP86 pKa = 3.69

Molecular weight: 9.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4497

0

4497

1332181

20

2169

296.2

32.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.523 ± 0.048

0.997 ± 0.01

5.497 ± 0.034

6.574 ± 0.037

3.853 ± 0.023

8.467 ± 0.04

2.095 ± 0.015

5.165 ± 0.025

3.696 ± 0.03

10.417 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.022

2.824 ± 0.02

4.883 ± 0.027

3.752 ± 0.022

6.168 ± 0.039

5.792 ± 0.026

5.092 ± 0.027

6.723 ± 0.03

1.414 ± 0.017

2.298 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski