Amino acid dipepetide frequency for Roseobacter sp. SK209-2-6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.683AlaAla: 14.683 ± 0.161
1.088AlaCys: 1.088 ± 0.03
5.869AlaAsp: 5.869 ± 0.077
8.717AlaGlu: 8.717 ± 0.116
3.986AlaPhe: 3.986 ± 0.063
9.929AlaGly: 9.929 ± 0.107
2.176AlaHis: 2.176 ± 0.041
5.591AlaIle: 5.591 ± 0.063
4.525AlaLys: 4.525 ± 0.067
12.973AlaLeu: 12.973 ± 0.143
3.484AlaMet: 3.484 ± 0.052
2.832AlaAsn: 2.832 ± 0.044
5.194AlaPro: 5.194 ± 0.087
4.531AlaGln: 4.531 ± 0.067
7.217AlaArg: 7.217 ± 0.083
6.003AlaSer: 6.003 ± 0.07
5.208AlaThr: 5.208 ± 0.073
7.32AlaVal: 7.32 ± 0.08
1.389AlaTrp: 1.389 ± 0.036
2.516AlaTyr: 2.516 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
1.12CysAla: 1.12 ± 0.029
0.151CysCys: 0.151 ± 0.011
0.609CysAsp: 0.609 ± 0.022
0.55CysGlu: 0.55 ± 0.02
0.439CysPhe: 0.439 ± 0.018
0.983CysGly: 0.983 ± 0.034
0.269CysHis: 0.269 ± 0.016
0.483CysIle: 0.483 ± 0.019
0.282CysLys: 0.282 ± 0.015
1.055CysLeu: 1.055 ± 0.031
0.196CysMet: 0.196 ± 0.012
0.282CysAsn: 0.282 ± 0.012
0.557CysPro: 0.557 ± 0.021
0.324CysGln: 0.324 ± 0.017
0.592CysArg: 0.592 ± 0.023
0.618CysSer: 0.618 ± 0.021
0.486CysThr: 0.486 ± 0.02
0.568CysVal: 0.568 ± 0.019
0.133CysTrp: 0.133 ± 0.011
0.27CysTyr: 0.27 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
5.773AspAla: 5.773 ± 0.071
0.619AspCys: 0.619 ± 0.022
2.639AspAsp: 2.639 ± 0.072
3.32AspGlu: 3.32 ± 0.056
2.329AspPhe: 2.329 ± 0.047
4.674AspGly: 4.674 ± 0.067
1.371AspHis: 1.371 ± 0.034
2.786AspIle: 2.786 ± 0.043
1.76AspLys: 1.76 ± 0.039
6.736AspLeu: 6.736 ± 0.083
1.567AspMet: 1.567 ± 0.032
1.3AspAsn: 1.3 ± 0.027
3.345AspPro: 3.345 ± 0.044
2.24AspGln: 2.24 ± 0.036
3.452AspArg: 3.452 ± 0.054
2.317AspSer: 2.317 ± 0.045
2.467AspThr: 2.467 ± 0.052
3.633AspVal: 3.633 ± 0.055
1.145AspTrp: 1.145 ± 0.035
1.498AspTyr: 1.498 ± 0.035
0.0AspXaa: 0.0 ± 0.0
Glu
8.727GluAla: 8.727 ± 0.116
0.435GluCys: 0.435 ± 0.019
3.638GluAsp: 3.638 ± 0.057
4.767GluGlu: 4.767 ± 0.08
2.157GluPhe: 2.157 ± 0.038
5.222GluGly: 5.222 ± 0.076
1.271GluHis: 1.271 ± 0.033
4.2GluIle: 4.2 ± 0.065
2.62GluLys: 2.62 ± 0.056
6.137GluLeu: 6.137 ± 0.08
2.017GluMet: 2.017 ± 0.036
2.09GluAsn: 2.09 ± 0.04
2.534GluPro: 2.534 ± 0.049
2.499GluGln: 2.499 ± 0.048
4.165GluArg: 4.165 ± 0.061
2.63GluSer: 2.63 ± 0.044
3.895GluThr: 3.895 ± 0.058
4.818GluVal: 4.818 ± 0.067
0.73GluTrp: 0.73 ± 0.021
1.191GluTyr: 1.191 ± 0.034
0.0GluXaa: 0.0 ± 0.0
Phe
4.465PheAla: 4.465 ± 0.069
0.52PheCys: 0.52 ± 0.022
2.771PheAsp: 2.771 ± 0.049
2.48PheGlu: 2.48 ± 0.042
1.561PhePhe: 1.561 ± 0.042
3.706PheGly: 3.706 ± 0.053
0.772PheHis: 0.772 ± 0.02
1.595PheIle: 1.595 ± 0.036
1.15PheLys: 1.15 ± 0.035
3.782PheLeu: 3.782 ± 0.066
0.91PheMet: 0.91 ± 0.026
1.144PheAsn: 1.144 ± 0.031
1.585PhePro: 1.585 ± 0.032
1.282PheGln: 1.282 ± 0.031
2.058PheArg: 2.058 ± 0.041
2.485PheSer: 2.485 ± 0.039
2.061PheThr: 2.061 ± 0.042
2.562PheVal: 2.562 ± 0.051
0.622PheTrp: 0.622 ± 0.025
1.022PheTyr: 1.022 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
8.934GlyAla: 8.934 ± 0.096
0.926GlyCys: 0.926 ± 0.026
4.35GlyAsp: 4.35 ± 0.074
5.147GlyGlu: 5.147 ± 0.065
3.902GlyPhe: 3.902 ± 0.062
7.143GlyGly: 7.143 ± 0.099
1.945GlyHis: 1.945 ± 0.041
4.469GlyIle: 4.469 ± 0.063
3.575GlyLys: 3.575 ± 0.058
8.991GlyLeu: 8.991 ± 0.099
2.487GlyMet: 2.487 ± 0.043
2.365GlyAsn: 2.365 ± 0.052
3.293GlyPro: 3.293 ± 0.051
3.399GlyGln: 3.399 ± 0.051
5.08GlyArg: 5.08 ± 0.069
4.714GlySer: 4.714 ± 0.057
4.283GlyThr: 4.283 ± 0.065
5.797GlyVal: 5.797 ± 0.062
1.401GlyTrp: 1.401 ± 0.037
2.469GlyTyr: 2.469 ± 0.043
0.0GlyXaa: 0.0 ± 0.0
His
2.114HisAla: 2.114 ± 0.044
0.281HisCys: 0.281 ± 0.014
1.143HisAsp: 1.143 ± 0.031
1.134HisGlu: 1.134 ± 0.028
0.942HisPhe: 0.942 ± 0.027
1.837HisGly: 1.837 ± 0.04
0.571HisHis: 0.571 ± 0.022
1.025HisIle: 1.025 ± 0.026
0.609HisLys: 0.609 ± 0.023
2.356HisLeu: 2.356 ± 0.044
0.586HisMet: 0.586 ± 0.02
0.503HisAsn: 0.503 ± 0.02
1.331HisPro: 1.331 ± 0.033
0.811HisGln: 0.811 ± 0.023
1.367HisArg: 1.367 ± 0.029
1.182HisSer: 1.182 ± 0.028
0.746HisThr: 0.746 ± 0.026
1.435HisVal: 1.435 ± 0.034
0.39HisTrp: 0.39 ± 0.018
0.589HisTyr: 0.589 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
6.549IleAla: 6.549 ± 0.068
0.698IleCys: 0.698 ± 0.022
3.084IleAsp: 3.084 ± 0.05
3.701IleGlu: 3.701 ± 0.06
1.799IlePhe: 1.799 ± 0.041
4.653IleGly: 4.653 ± 0.075
0.909IleHis: 0.909 ± 0.028
2.199IleIle: 2.199 ± 0.052
1.714IleLys: 1.714 ± 0.037
5.189IleLeu: 5.189 ± 0.076
1.164IleMet: 1.164 ± 0.034
1.495IleAsn: 1.495 ± 0.033
2.355IlePro: 2.355 ± 0.048
1.449IleGln: 1.449 ± 0.033
2.994IleArg: 2.994 ± 0.046
3.589IleSer: 3.589 ± 0.054
2.849IleThr: 2.849 ± 0.047
3.193IleVal: 3.193 ± 0.052
0.806IleTrp: 0.806 ± 0.027
1.261IleTyr: 1.261 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
4.369LysAla: 4.369 ± 0.066
0.243LysCys: 0.243 ± 0.015
2.025LysAsp: 2.025 ± 0.048
2.252LysGlu: 2.252 ± 0.048
1.129LysPhe: 1.129 ± 0.027
3.12LysGly: 3.12 ± 0.056
0.724LysHis: 0.724 ± 0.023
1.987LysIle: 1.987 ± 0.043
1.504LysLys: 1.504 ± 0.043
3.512LysLeu: 3.512 ± 0.053
1.049LysMet: 1.049 ± 0.029
1.029LysAsn: 1.029 ± 0.03
2.078LysPro: 2.078 ± 0.046
1.323LysGln: 1.323 ± 0.031
2.52LysArg: 2.52 ± 0.05
2.276LysSer: 2.276 ± 0.042
2.217LysThr: 2.217 ± 0.043
2.472LysVal: 2.472 ± 0.048
0.414LysTrp: 0.414 ± 0.017
0.718LysTyr: 0.718 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
12.353LeuAla: 12.353 ± 0.141
1.09LeuCys: 1.09 ± 0.031
5.624LeuAsp: 5.624 ± 0.063
6.549LeuGlu: 6.549 ± 0.079
3.859LeuPhe: 3.859 ± 0.068
8.54LeuGly: 8.54 ± 0.112
2.01LeuHis: 2.01 ± 0.037
5.258LeuIle: 5.258 ± 0.071
3.971LeuLys: 3.971 ± 0.061
9.74LeuLeu: 9.74 ± 0.141
2.766LeuMet: 2.766 ± 0.051
3.009LeuAsn: 3.009 ± 0.048
5.778LeuPro: 5.778 ± 0.083
3.739LeuGln: 3.739 ± 0.051
6.89LeuArg: 6.89 ± 0.084
7.266LeuSer: 7.266 ± 0.079
5.709LeuThr: 5.709 ± 0.071
6.657LeuVal: 6.657 ± 0.087
1.35LeuTrp: 1.35 ± 0.039
2.012LeuTyr: 2.012 ± 0.044
0.0LeuXaa: 0.0 ± 0.0
Met
3.243MetAla: 3.243 ± 0.05
0.218MetCys: 0.218 ± 0.013
1.325MetAsp: 1.325 ± 0.031
1.534MetGlu: 1.534 ± 0.036
0.805MetPhe: 0.805 ± 0.029
2.27MetGly: 2.27 ± 0.048
0.527MetHis: 0.527 ± 0.019
1.494MetIle: 1.494 ± 0.033
1.155MetLys: 1.155 ± 0.028
2.688MetLeu: 2.688 ± 0.054
0.82MetMet: 0.82 ± 0.028
0.854MetAsn: 0.854 ± 0.024
1.47MetPro: 1.47 ± 0.036
1.203MetGln: 1.203 ± 0.032
1.819MetArg: 1.819 ± 0.04
1.91MetSer: 1.91 ± 0.034
1.94MetThr: 1.94 ± 0.04
1.837MetVal: 1.837 ± 0.039
0.245MetTrp: 0.245 ± 0.013
0.33MetTyr: 0.33 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.187AsnAla: 3.187 ± 0.049
0.323AsnCys: 0.323 ± 0.015
1.483AsnAsp: 1.483 ± 0.041
1.398AsnGlu: 1.398 ± 0.033
1.065AsnPhe: 1.065 ± 0.029
2.512AsnGly: 2.512 ± 0.048
0.59AsnHis: 0.59 ± 0.021
1.439AsnIle: 1.439 ± 0.039
0.881AsnLys: 0.881 ± 0.029
3.028AsnLeu: 3.028 ± 0.045
0.789AsnMet: 0.789 ± 0.024
0.783AsnAsn: 0.783 ± 0.026
1.946AsnPro: 1.946 ± 0.04
0.999AsnGln: 0.999 ± 0.029
1.816AsnArg: 1.816 ± 0.039
1.565AsnSer: 1.565 ± 0.037
1.397AsnThr: 1.397 ± 0.034
1.751AsnVal: 1.751 ± 0.038
0.55AsnTrp: 0.55 ± 0.018
0.745AsnTyr: 0.745 ± 0.024
0.0AsnXaa: 0.0 ± 0.0
Pro
5.211ProAla: 5.211 ± 0.076
0.384ProCys: 0.384 ± 0.015
3.202ProAsp: 3.202 ± 0.046
4.402ProGlu: 4.402 ± 0.072
1.881ProPhe: 1.881 ± 0.039
4.236ProGly: 4.236 ± 0.061
1.024ProHis: 1.024 ± 0.029
2.207ProIle: 2.207 ± 0.045
1.972ProLys: 1.972 ± 0.042
4.769ProLeu: 4.769 ± 0.075
1.308ProMet: 1.308 ± 0.031
1.419ProAsn: 1.419 ± 0.036
2.006ProPro: 2.006 ± 0.044
1.853ProGln: 1.853 ± 0.044
2.429ProArg: 2.429 ± 0.048
2.768ProSer: 2.768 ± 0.043
2.028ProThr: 2.028 ± 0.035
3.897ProVal: 3.897 ± 0.056
0.666ProTrp: 0.666 ± 0.025
1.163ProTyr: 1.163 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
4.653GlnAla: 4.653 ± 0.065
0.279GlnCys: 0.279 ± 0.015
2.006GlnAsp: 2.006 ± 0.043
2.439GlnGlu: 2.439 ± 0.045
1.222GlnPhe: 1.222 ± 0.032
3.022GlnGly: 3.022 ± 0.055
0.724GlnHis: 0.724 ± 0.022
2.329GlnIle: 2.329 ± 0.044
1.497GlnLys: 1.497 ± 0.032
3.441GlnLeu: 3.441 ± 0.046
1.218GlnMet: 1.218 ± 0.031
1.188GlnAsn: 1.188 ± 0.027
1.714GlnPro: 1.714 ± 0.042
1.474GlnGln: 1.474 ± 0.038
2.503GlnArg: 2.503 ± 0.05
2.354GlnSer: 2.354 ± 0.044
1.809GlnThr: 1.809 ± 0.038
2.577GlnVal: 2.577 ± 0.047
0.431GlnTrp: 0.431 ± 0.018
0.639GlnTyr: 0.639 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
6.677ArgAla: 6.677 ± 0.076
0.537ArgCys: 0.537 ± 0.023
3.534ArgAsp: 3.534 ± 0.055
3.945ArgGlu: 3.945 ± 0.062
2.578ArgPhe: 2.578 ± 0.047
4.156ArgGly: 4.156 ± 0.06
1.442ArgHis: 1.442 ± 0.03
3.601ArgIle: 3.601 ± 0.053
2.459ArgLys: 2.459 ± 0.047
6.819ArgLeu: 6.819 ± 0.079
1.799ArgMet: 1.799 ± 0.033
1.889ArgAsn: 1.889 ± 0.038
2.869ArgPro: 2.869 ± 0.049
2.593ArgGln: 2.593 ± 0.047
4.397ArgArg: 4.397 ± 0.068
3.462ArgSer: 3.462 ± 0.055
2.536ArgThr: 2.536 ± 0.039
4.058ArgVal: 4.058 ± 0.059
0.887ArgTrp: 0.887 ± 0.028
1.443ArgTyr: 1.443 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
6.289SerAla: 6.289 ± 0.083
0.611SerCys: 0.611 ± 0.023
3.329SerAsp: 3.329 ± 0.053
3.61SerGlu: 3.61 ± 0.055
2.612SerPhe: 2.612 ± 0.044
5.749SerGly: 5.749 ± 0.073
1.32SerHis: 1.32 ± 0.033
2.886SerIle: 2.886 ± 0.052
2.103SerLys: 2.103 ± 0.043
5.82SerLeu: 5.82 ± 0.079
1.536SerMet: 1.536 ± 0.038
1.658SerAsn: 1.658 ± 0.035
2.779SerPro: 2.779 ± 0.043
2.108SerGln: 2.108 ± 0.041
3.315SerArg: 3.315 ± 0.059
3.34SerSer: 3.34 ± 0.057
2.756SerThr: 2.756 ± 0.048
3.713SerVal: 3.713 ± 0.05
0.82SerTrp: 0.82 ± 0.024
1.564SerTyr: 1.564 ± 0.037
0.0SerXaa: 0.0 ± 0.0
Thr
5.659ThrAla: 5.659 ± 0.071
0.517ThrCys: 0.517 ± 0.02
2.626ThrAsp: 2.626 ± 0.052
3.0ThrGlu: 3.0 ± 0.045
1.817ThrPhe: 1.817 ± 0.035
5.056ThrGly: 5.056 ± 0.065
1.112ThrHis: 1.112 ± 0.031
2.461ThrIle: 2.461 ± 0.042
1.64ThrLys: 1.64 ± 0.04
5.724ThrLeu: 5.724 ± 0.069
1.201ThrMet: 1.201 ± 0.029
1.294ThrAsn: 1.294 ± 0.038
3.045ThrPro: 3.045 ± 0.047
1.744ThrGln: 1.744 ± 0.037
3.061ThrArg: 3.061 ± 0.044
2.916ThrSer: 2.916 ± 0.049
2.561ThrThr: 2.561 ± 0.058
3.563ThrVal: 3.563 ± 0.056
0.693ThrTrp: 0.693 ± 0.021
1.231ThrTyr: 1.231 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
7.441ValAla: 7.441 ± 0.069
0.607ValCys: 0.607 ± 0.021
3.491ValAsp: 3.491 ± 0.056
4.604ValGlu: 4.604 ± 0.069
2.847ValPhe: 2.847 ± 0.05
4.713ValGly: 4.713 ± 0.061
1.254ValHis: 1.254 ± 0.03
3.943ValIle: 3.943 ± 0.064
2.335ValLys: 2.335 ± 0.044
7.307ValLeu: 7.307 ± 0.089
1.883ValMet: 1.883 ± 0.039
1.976ValAsn: 1.976 ± 0.042
3.266ValPro: 3.266 ± 0.058
2.315ValGln: 2.315 ± 0.043
3.442ValArg: 3.442 ± 0.049
4.262ValSer: 4.262 ± 0.059
4.288ValThr: 4.288 ± 0.06
5.001ValVal: 5.001 ± 0.064
0.884ValTrp: 0.884 ± 0.029
1.372ValTyr: 1.372 ± 0.031
0.0ValXaa: 0.0 ± 0.0
Trp
1.322TrpAla: 1.322 ± 0.034
0.16TrpCys: 0.16 ± 0.011
0.724TrpAsp: 0.724 ± 0.028
0.763TrpGlu: 0.763 ± 0.024
0.613TrpPhe: 0.613 ± 0.021
1.055TrpGly: 1.055 ± 0.029
0.38TrpHis: 0.38 ± 0.017
0.771TrpIle: 0.771 ± 0.026
0.515TrpLys: 0.515 ± 0.02
1.64TrpLeu: 1.64 ± 0.036
0.428TrpMet: 0.428 ± 0.019
0.462TrpAsn: 0.462 ± 0.019
0.644TrpPro: 0.644 ± 0.02
0.728TrpGln: 0.728 ± 0.023
1.049TrpArg: 1.049 ± 0.031
0.875TrpSer: 0.875 ± 0.026
0.652TrpThr: 0.652 ± 0.023
0.866TrpVal: 0.866 ± 0.024
0.201TrpTrp: 0.201 ± 0.013
0.299TrpTyr: 0.299 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.463TyrAla: 2.463 ± 0.043
0.279TyrCys: 0.279 ± 0.015
1.501TyrAsp: 1.501 ± 0.031
1.429TyrGlu: 1.429 ± 0.032
0.987TyrPhe: 0.987 ± 0.03
2.096TyrGly: 2.096 ± 0.047
0.56TyrHis: 0.56 ± 0.023
0.927TyrIle: 0.927 ± 0.028
0.695TyrLys: 0.695 ± 0.023
2.463TyrLeu: 2.463 ± 0.044
0.477TyrMet: 0.477 ± 0.019
0.672TyrAsn: 0.672 ± 0.023
1.036TyrPro: 1.036 ± 0.03
0.906TyrGln: 0.906 ± 0.03
1.516TyrArg: 1.516 ± 0.037
1.389TyrSer: 1.389 ± 0.032
1.033TyrThr: 1.033 ± 0.026
1.514TyrVal: 1.514 ± 0.036
0.389TyrTrp: 0.389 ± 0.018
0.648TyrTyr: 0.648 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4497 proteins (1332181 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski