Lactobacillus phage JCL1032

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8J1E4|F8J1E4_9CAUD Uncharacterized protein OS=Lactobacillus phage JCL1032 OX=37105 PE=4 SV=1
MM1 pKa = 7.19NVIEE5 pKa = 4.54LHH7 pKa = 5.68PAEE10 pKa = 4.41NEE12 pKa = 3.91EE13 pKa = 4.09MDD15 pKa = 3.73EE16 pKa = 4.39MEE18 pKa = 4.85YY19 pKa = 10.69TPMNYY24 pKa = 9.03IMALLNPDD32 pKa = 4.03HH33 pKa = 7.06PPVVDD38 pKa = 3.54GRR40 pKa = 11.84EE41 pKa = 3.85MTDD44 pKa = 3.64YY45 pKa = 11.21EE46 pKa = 3.89ILEE49 pKa = 4.17YY50 pKa = 10.73LRR52 pKa = 11.84QCVYY56 pKa = 11.23

Molecular weight: 6.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8J1G4|F8J1G4_9CAUD Uncharacterized protein OS=Lactobacillus phage JCL1032 OX=37105 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84WNRR5 pKa = 11.84TSFQPKK11 pKa = 9.12HH12 pKa = 5.57NCNKK16 pKa = 10.45KK17 pKa = 9.92IMRR20 pKa = 11.84SSTKK24 pKa = 10.0FKK26 pKa = 10.94LQFTICCSRR35 pKa = 11.84LSTPITAMHH44 pKa = 7.24DD45 pKa = 3.38LGLAPSGSLWCRR57 pKa = 11.84VTT59 pKa = 3.61

Molecular weight: 6.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

14870

32

1868

193.1

21.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.814 ± 0.559

0.941 ± 0.186

6.597 ± 0.436

5.992 ± 0.349

3.712 ± 0.244

6.799 ± 0.415

1.923 ± 0.144

5.548 ± 0.217

8.016 ± 0.592

8.117 ± 0.32

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.845 ± 0.154

5.185 ± 0.242

3.45 ± 0.275

3.927 ± 0.201

4.519 ± 0.252

6.564 ± 0.272

6.18 ± 0.284

6.301 ± 0.193

1.533 ± 0.125

4.035 ± 0.301
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski