Amino acid dipepetide frequency for Lactobacillus phage JCL1032

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.322AlaAla: 6.322 ± 1.05
0.942AlaCys: 0.942 ± 0.268
5.38AlaAsp: 5.38 ± 0.679
4.977AlaGlu: 4.977 ± 0.749
3.161AlaPhe: 3.161 ± 0.523
5.784AlaGly: 5.784 ± 1.036
1.614AlaHis: 1.614 ± 0.264
4.237AlaIle: 4.237 ± 0.487
6.053AlaLys: 6.053 ± 1.046
6.187AlaLeu: 6.187 ± 0.77
2.287AlaMet: 2.287 ± 0.317
4.641AlaAsn: 4.641 ± 0.663
2.354AlaPro: 2.354 ± 0.589
2.69AlaGln: 2.69 ± 0.551
3.766AlaArg: 3.766 ± 0.428
4.842AlaSer: 4.842 ± 0.709
3.497AlaThr: 3.497 ± 0.565
4.775AlaVal: 4.775 ± 0.573
1.547AlaTrp: 1.547 ± 0.31
3.094AlaTyr: 3.094 ± 0.451
0.0AlaXaa: 0.0 ± 0.0
Cys
0.404CysAla: 0.404 ± 0.211
0.269CysCys: 0.269 ± 0.192
0.673CysAsp: 0.673 ± 0.216
0.404CysGlu: 0.404 ± 0.175
0.269CysPhe: 0.269 ± 0.124
0.538CysGly: 0.538 ± 0.193
0.135CysHis: 0.135 ± 0.094
0.202CysIle: 0.202 ± 0.124
1.076CysLys: 1.076 ± 0.318
1.211CysLeu: 1.211 ± 0.39
0.538CysMet: 0.538 ± 0.227
0.269CysAsn: 0.269 ± 0.153
0.404CysPro: 0.404 ± 0.17
0.336CysGln: 0.336 ± 0.143
0.336CysArg: 0.336 ± 0.179
0.74CysSer: 0.74 ± 0.32
0.471CysThr: 0.471 ± 0.213
0.538CysVal: 0.538 ± 0.21
0.135CysTrp: 0.135 ± 0.096
0.471CysTyr: 0.471 ± 0.155
0.0CysXaa: 0.0 ± 0.0
Asp
4.842AspAla: 4.842 ± 0.578
0.605AspCys: 0.605 ± 0.188
5.38AspAsp: 5.38 ± 1.053
4.17AspGlu: 4.17 ± 0.619
2.892AspPhe: 2.892 ± 0.442
4.573AspGly: 4.573 ± 0.693
1.143AspHis: 1.143 ± 0.32
3.228AspIle: 3.228 ± 0.452
5.179AspLys: 5.179 ± 0.603
5.582AspLeu: 5.582 ± 0.653
2.219AspMet: 2.219 ± 0.274
3.43AspAsn: 3.43 ± 0.517
2.018AspPro: 2.018 ± 0.414
2.825AspGln: 2.825 ± 0.417
2.287AspArg: 2.287 ± 0.429
3.632AspSer: 3.632 ± 0.477
3.564AspThr: 3.564 ± 0.539
3.766AspVal: 3.766 ± 0.45
1.211AspTrp: 1.211 ± 0.344
3.43AspTyr: 3.43 ± 0.683
0.0AspXaa: 0.0 ± 0.0
Glu
4.775GluAla: 4.775 ± 0.576
0.74GluCys: 0.74 ± 0.268
2.892GluAsp: 2.892 ± 0.529
3.766GluGlu: 3.766 ± 0.619
2.757GluPhe: 2.757 ± 0.408
4.506GluGly: 4.506 ± 0.7
1.076GluHis: 1.076 ± 0.244
3.632GluIle: 3.632 ± 0.542
4.439GluLys: 4.439 ± 0.549
5.717GluLeu: 5.717 ± 0.705
2.354GluMet: 2.354 ± 0.448
2.219GluAsn: 2.219 ± 0.391
1.681GluPro: 1.681 ± 0.451
2.959GluGln: 2.959 ± 0.513
2.219GluArg: 2.219 ± 0.466
3.363GluSer: 3.363 ± 0.435
2.892GluThr: 2.892 ± 0.38
3.766GluVal: 3.766 ± 0.567
0.874GluTrp: 0.874 ± 0.225
3.295GluTyr: 3.295 ± 0.52
0.0GluXaa: 0.0 ± 0.0
Phe
2.421PheAla: 2.421 ± 0.332
0.336PheCys: 0.336 ± 0.132
2.623PheAsp: 2.623 ± 0.516
2.623PheGlu: 2.623 ± 0.588
1.614PhePhe: 1.614 ± 0.343
2.623PheGly: 2.623 ± 0.354
0.202PheHis: 0.202 ± 0.121
2.421PheIle: 2.421 ± 0.387
3.43PheLys: 3.43 ± 0.367
2.959PheLeu: 2.959 ± 0.501
1.278PheMet: 1.278 ± 0.312
2.354PheAsn: 2.354 ± 0.404
0.807PhePro: 0.807 ± 0.245
1.278PheGln: 1.278 ± 0.323
1.547PheArg: 1.547 ± 0.276
3.161PheSer: 3.161 ± 0.478
1.816PheThr: 1.816 ± 0.315
1.816PheVal: 1.816 ± 0.384
0.269PheTrp: 0.269 ± 0.128
1.547PheTyr: 1.547 ± 0.342
0.0PheXaa: 0.0 ± 0.0
Gly
4.91GlyAla: 4.91 ± 1.019
0.202GlyCys: 0.202 ± 0.124
5.179GlyAsp: 5.179 ± 0.93
3.699GlyGlu: 3.699 ± 0.458
2.556GlyPhe: 2.556 ± 0.517
4.439GlyGly: 4.439 ± 0.64
1.278GlyHis: 1.278 ± 0.279
3.161GlyIle: 3.161 ± 0.379
5.649GlyLys: 5.649 ± 0.566
6.793GlyLeu: 6.793 ± 0.92
1.345GlyMet: 1.345 ± 0.381
3.632GlyAsn: 3.632 ± 0.496
2.219GlyPro: 2.219 ± 0.455
2.488GlyGln: 2.488 ± 0.408
2.825GlyArg: 2.825 ± 0.471
3.766GlySer: 3.766 ± 0.455
4.17GlyThr: 4.17 ± 0.664
5.111GlyVal: 5.111 ± 0.851
1.345GlyTrp: 1.345 ± 0.234
3.228GlyTyr: 3.228 ± 0.534
0.0GlyXaa: 0.0 ± 0.0
His
0.942HisAla: 0.942 ± 0.215
0.202HisCys: 0.202 ± 0.118
1.345HisAsp: 1.345 ± 0.261
1.48HisGlu: 1.48 ± 0.321
1.009HisPhe: 1.009 ± 0.291
1.211HisGly: 1.211 ± 0.303
0.605HisHis: 0.605 ± 0.197
1.211HisIle: 1.211 ± 0.264
1.076HisLys: 1.076 ± 0.262
1.076HisLeu: 1.076 ± 0.225
0.605HisMet: 0.605 ± 0.181
1.076HisAsn: 1.076 ± 0.295
1.009HisPro: 1.009 ± 0.35
0.135HisGln: 0.135 ± 0.088
0.942HisArg: 0.942 ± 0.206
0.605HisSer: 0.605 ± 0.192
1.48HisThr: 1.48 ± 0.368
1.883HisVal: 1.883 ± 0.396
0.202HisTrp: 0.202 ± 0.146
1.143HisTyr: 1.143 ± 0.333
0.0HisXaa: 0.0 ± 0.0
Ile
3.632IleAla: 3.632 ± 0.455
0.673IleCys: 0.673 ± 0.236
4.372IleAsp: 4.372 ± 0.495
3.564IleGlu: 3.564 ± 0.409
1.345IlePhe: 1.345 ± 0.258
3.497IleGly: 3.497 ± 0.537
1.076IleHis: 1.076 ± 0.213
2.354IleIle: 2.354 ± 0.564
5.38IleLys: 5.38 ± 0.689
3.766IleLeu: 3.766 ± 0.539
1.48IleMet: 1.48 ± 0.315
3.564IleAsn: 3.564 ± 0.537
2.354IlePro: 2.354 ± 0.427
2.018IleGln: 2.018 ± 0.337
2.421IleArg: 2.421 ± 0.475
3.833IleSer: 3.833 ± 0.461
4.372IleThr: 4.372 ± 0.684
3.094IleVal: 3.094 ± 0.524
1.009IleTrp: 1.009 ± 0.262
1.681IleTyr: 1.681 ± 0.33
0.0IleXaa: 0.0 ± 0.0
Lys
8.205LysAla: 8.205 ± 1.135
0.471LysCys: 0.471 ± 0.185
4.708LysAsp: 4.708 ± 0.629
5.313LysGlu: 5.313 ± 0.762
3.026LysPhe: 3.026 ± 0.494
4.775LysGly: 4.775 ± 0.686
2.085LysHis: 2.085 ± 0.381
4.035LysIle: 4.035 ± 0.439
6.658LysLys: 6.658 ± 0.968
6.322LysLeu: 6.322 ± 0.685
2.757LysMet: 2.757 ± 0.433
3.632LysAsn: 3.632 ± 0.427
2.623LysPro: 2.623 ± 0.592
3.363LysGln: 3.363 ± 0.611
3.766LysArg: 3.766 ± 0.552
5.044LysSer: 5.044 ± 0.949
5.111LysThr: 5.111 ± 0.649
4.17LysVal: 4.17 ± 0.566
1.076LysTrp: 1.076 ± 0.25
2.959LysTyr: 2.959 ± 0.495
0.0LysXaa: 0.0 ± 0.0
Leu
7.801LeuAla: 7.801 ± 0.823
0.673LeuCys: 0.673 ± 0.24
5.38LeuAsp: 5.38 ± 0.6
3.901LeuGlu: 3.901 ± 0.629
2.892LeuPhe: 2.892 ± 0.531
5.717LeuGly: 5.717 ± 0.639
1.345LeuHis: 1.345 ± 0.354
4.102LeuIle: 4.102 ± 0.481
6.053LeuLys: 6.053 ± 0.816
5.515LeuLeu: 5.515 ± 0.828
2.421LeuMet: 2.421 ± 0.325
3.564LeuAsn: 3.564 ± 0.554
2.959LeuPro: 2.959 ± 0.504
2.959LeuGln: 2.959 ± 0.507
4.304LeuArg: 4.304 ± 0.585
6.12LeuSer: 6.12 ± 0.717
6.053LeuThr: 6.053 ± 0.569
5.38LeuVal: 5.38 ± 0.763
0.874LeuTrp: 0.874 ± 0.239
3.161LeuTyr: 3.161 ± 0.551
0.0LeuXaa: 0.0 ± 0.0
Met
2.623MetAla: 2.623 ± 0.355
0.135MetCys: 0.135 ± 0.102
1.749MetAsp: 1.749 ± 0.337
1.48MetGlu: 1.48 ± 0.283
0.874MetPhe: 0.874 ± 0.244
1.614MetGly: 1.614 ± 0.412
0.538MetHis: 0.538 ± 0.18
2.085MetIle: 2.085 ± 0.33
2.488MetLys: 2.488 ± 0.376
2.959MetLeu: 2.959 ± 0.362
0.807MetMet: 0.807 ± 0.259
1.412MetAsn: 1.412 ± 0.309
0.605MetPro: 0.605 ± 0.226
1.143MetGln: 1.143 ± 0.256
1.48MetArg: 1.48 ± 0.325
2.354MetSer: 2.354 ± 0.485
1.412MetThr: 1.412 ± 0.307
1.816MetVal: 1.816 ± 0.314
0.404MetTrp: 0.404 ± 0.174
0.471MetTyr: 0.471 ± 0.181
0.0MetXaa: 0.0 ± 0.0
Asn
4.102AsnAla: 4.102 ± 0.663
0.404AsnCys: 0.404 ± 0.195
3.564AsnAsp: 3.564 ± 0.621
3.632AsnGlu: 3.632 ± 0.529
1.95AsnPhe: 1.95 ± 0.425
5.313AsnGly: 5.313 ± 0.601
1.412AsnHis: 1.412 ± 0.359
2.085AsnIle: 2.085 ± 0.381
3.161AsnLys: 3.161 ± 0.532
3.766AsnLeu: 3.766 ± 0.518
1.412AsnMet: 1.412 ± 0.28
2.69AsnAsn: 2.69 ± 0.361
2.354AsnPro: 2.354 ± 0.381
1.547AsnGln: 1.547 ± 0.331
1.883AsnArg: 1.883 ± 0.442
3.094AsnSer: 3.094 ± 0.485
2.556AsnThr: 2.556 ± 0.402
3.632AsnVal: 3.632 ± 0.477
1.009AsnTrp: 1.009 ± 0.284
2.287AsnTyr: 2.287 ± 0.381
0.0AsnXaa: 0.0 ± 0.0
Pro
3.026ProAla: 3.026 ± 0.597
0.202ProCys: 0.202 ± 0.145
2.556ProAsp: 2.556 ± 0.472
2.354ProGlu: 2.354 ± 0.414
1.009ProPhe: 1.009 ± 0.28
2.152ProGly: 2.152 ± 0.429
0.673ProHis: 0.673 ± 0.165
2.556ProIle: 2.556 ± 0.544
2.69ProLys: 2.69 ± 0.47
3.161ProLeu: 3.161 ± 0.578
0.605ProMet: 0.605 ± 0.214
1.681ProAsn: 1.681 ± 0.458
0.538ProPro: 0.538 ± 0.205
1.278ProGln: 1.278 ± 0.353
1.345ProArg: 1.345 ± 0.315
2.085ProSer: 2.085 ± 0.386
2.825ProThr: 2.825 ± 0.48
2.085ProVal: 2.085 ± 0.373
0.673ProTrp: 0.673 ± 0.261
1.009ProTyr: 1.009 ± 0.316
0.0ProXaa: 0.0 ± 0.0
Gln
3.901GlnAla: 3.901 ± 0.586
0.538GlnCys: 0.538 ± 0.176
1.816GlnAsp: 1.816 ± 0.4
2.488GlnGlu: 2.488 ± 0.376
1.278GlnPhe: 1.278 ± 0.229
1.749GlnGly: 1.749 ± 0.244
1.009GlnHis: 1.009 ± 0.264
2.354GlnIle: 2.354 ± 0.304
3.094GlnLys: 3.094 ± 0.505
3.094GlnLeu: 3.094 ± 0.425
1.143GlnMet: 1.143 ± 0.289
1.883GlnAsn: 1.883 ± 0.384
1.614GlnPro: 1.614 ± 0.499
1.749GlnGln: 1.749 ± 0.371
1.816GlnArg: 1.816 ± 0.378
1.95GlnSer: 1.95 ± 0.367
2.959GlnThr: 2.959 ± 0.518
2.556GlnVal: 2.556 ± 0.428
0.538GlnTrp: 0.538 ± 0.228
1.749GlnTyr: 1.749 ± 0.332
0.0GlnXaa: 0.0 ± 0.0
Arg
2.757ArgAla: 2.757 ± 0.541
0.202ArgCys: 0.202 ± 0.144
2.623ArgAsp: 2.623 ± 0.371
3.094ArgGlu: 3.094 ± 0.569
2.018ArgPhe: 2.018 ± 0.572
2.152ArgGly: 2.152 ± 0.383
1.009ArgHis: 1.009 ± 0.305
2.757ArgIle: 2.757 ± 0.399
3.564ArgLys: 3.564 ± 0.57
4.237ArgLeu: 4.237 ± 0.468
1.547ArgMet: 1.547 ± 0.267
2.421ArgAsn: 2.421 ± 0.388
1.48ArgPro: 1.48 ± 0.346
2.556ArgGln: 2.556 ± 0.454
2.287ArgArg: 2.287 ± 0.415
2.825ArgSer: 2.825 ± 0.427
2.488ArgThr: 2.488 ± 0.416
2.556ArgVal: 2.556 ± 0.468
0.942ArgTrp: 0.942 ± 0.261
1.681ArgTyr: 1.681 ± 0.309
0.0ArgXaa: 0.0 ± 0.0
Ser
5.179SerAla: 5.179 ± 0.507
0.336SerCys: 0.336 ± 0.14
3.833SerAsp: 3.833 ± 0.561
3.564SerGlu: 3.564 ± 0.516
1.95SerPhe: 1.95 ± 0.346
4.372SerGly: 4.372 ± 0.868
0.942SerHis: 0.942 ± 0.262
4.035SerIle: 4.035 ± 0.726
5.851SerLys: 5.851 ± 0.675
4.237SerLeu: 4.237 ± 0.387
1.749SerMet: 1.749 ± 0.334
3.295SerAsn: 3.295 ± 0.466
2.556SerPro: 2.556 ± 0.406
3.497SerGln: 3.497 ± 0.511
3.699SerArg: 3.699 ± 0.539
4.641SerSer: 4.641 ± 0.61
4.035SerThr: 4.035 ± 0.554
4.102SerVal: 4.102 ± 0.496
1.009SerTrp: 1.009 ± 0.259
2.757SerTyr: 2.757 ± 0.356
0.0SerXaa: 0.0 ± 0.0
Thr
4.439ThrAla: 4.439 ± 0.521
0.807ThrCys: 0.807 ± 0.259
4.439ThrAsp: 4.439 ± 0.686
3.161ThrGlu: 3.161 ± 0.345
1.883ThrPhe: 1.883 ± 0.365
4.775ThrGly: 4.775 ± 0.549
1.009ThrHis: 1.009 ± 0.242
4.17ThrIle: 4.17 ± 0.37
4.708ThrLys: 4.708 ± 0.926
4.91ThrLeu: 4.91 ± 0.573
1.143ThrMet: 1.143 ± 0.238
2.825ThrAsn: 2.825 ± 0.39
2.757ThrPro: 2.757 ± 0.552
1.883ThrGln: 1.883 ± 0.432
2.152ThrArg: 2.152 ± 0.458
4.775ThrSer: 4.775 ± 0.637
4.439ThrThr: 4.439 ± 0.67
4.708ThrVal: 4.708 ± 0.526
0.74ThrTrp: 0.74 ± 0.211
2.085ThrTyr: 2.085 ± 0.388
0.0ThrXaa: 0.0 ± 0.0
Val
4.237ValAla: 4.237 ± 0.488
0.673ValCys: 0.673 ± 0.252
3.564ValAsp: 3.564 ± 0.369
3.295ValGlu: 3.295 ± 0.452
2.556ValPhe: 2.556 ± 0.412
4.237ValGly: 4.237 ± 0.469
0.807ValHis: 0.807 ± 0.207
4.035ValIle: 4.035 ± 0.631
6.053ValLys: 6.053 ± 0.621
4.641ValLeu: 4.641 ± 0.647
1.614ValMet: 1.614 ± 0.326
3.901ValAsn: 3.901 ± 0.452
2.354ValPro: 2.354 ± 0.386
2.085ValGln: 2.085 ± 0.347
2.757ValArg: 2.757 ± 0.422
5.448ValSer: 5.448 ± 0.528
4.641ValThr: 4.641 ± 0.577
3.295ValVal: 3.295 ± 0.414
0.605ValTrp: 0.605 ± 0.228
2.219ValTyr: 2.219 ± 0.379
0.0ValXaa: 0.0 ± 0.0
Trp
1.009TrpAla: 1.009 ± 0.235
0.336TrpCys: 0.336 ± 0.152
1.009TrpAsp: 1.009 ± 0.217
0.807TrpGlu: 0.807 ± 0.286
0.605TrpPhe: 0.605 ± 0.17
0.942TrpGly: 0.942 ± 0.271
0.404TrpHis: 0.404 ± 0.172
0.807TrpIle: 0.807 ± 0.245
0.807TrpLys: 0.807 ± 0.215
1.345TrpLeu: 1.345 ± 0.331
0.336TrpMet: 0.336 ± 0.127
1.412TrpAsn: 1.412 ± 0.287
0.605TrpPro: 0.605 ± 0.214
0.673TrpGln: 0.673 ± 0.211
0.74TrpArg: 0.74 ± 0.267
0.605TrpSer: 0.605 ± 0.279
1.076TrpThr: 1.076 ± 0.206
1.143TrpVal: 1.143 ± 0.328
0.067TrpTrp: 0.067 ± 0.069
0.605TrpTyr: 0.605 ± 0.206
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.623TyrAla: 2.623 ± 0.471
0.673TyrCys: 0.673 ± 0.243
2.892TyrAsp: 2.892 ± 0.534
2.152TyrGlu: 2.152 ± 0.415
1.48TyrPhe: 1.48 ± 0.332
3.026TyrGly: 3.026 ± 0.53
0.874TyrHis: 0.874 ± 0.301
2.018TyrIle: 2.018 ± 0.322
2.757TyrLys: 2.757 ± 0.52
3.699TyrLeu: 3.699 ± 0.819
0.74TyrMet: 0.74 ± 0.198
1.95TyrAsn: 1.95 ± 0.391
1.211TyrPro: 1.211 ± 0.293
1.816TyrGln: 1.816 ± 0.467
2.623TyrArg: 2.623 ± 0.38
2.757TyrSer: 2.757 ± 0.433
1.95TyrThr: 1.95 ± 0.449
2.825TyrVal: 2.825 ± 0.547
0.807TyrTrp: 0.807 ± 0.216
1.48TyrTyr: 1.48 ± 0.345
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 77 proteins (14870 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski