Spinach yellow vein Sikar virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Spinach yellow vein virus

Average proteome isoelectric point is 7.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5SNH6|V5SNH6_9GEMI Capsid protein OS=Spinach yellow vein Sikar virus OX=1432565 GN=AV1 PE=3 SV=1
MM1 pKa = 7.45PRR3 pKa = 11.84INSFCVNAKK12 pKa = 10.49NIFLTYY18 pKa = 9.31FHH20 pKa = 6.83TPIPIFILLEE30 pKa = 3.7ILQSINCPSDD40 pKa = 3.49NPCFRR45 pKa = 11.84VAQEE49 pKa = 3.82KK50 pKa = 9.77HH51 pKa = 5.22QDD53 pKa = 3.38GSLHH57 pKa = 5.16IHH59 pKa = 6.91ALIQFKK65 pKa = 10.72GKK67 pKa = 10.47SKK69 pKa = 10.22FRR71 pKa = 11.84NPRR74 pKa = 11.84HH75 pKa = 6.17FDD77 pKa = 3.03VTHH80 pKa = 7.16PNNSTQFHH88 pKa = 7.21PNFQGAKK95 pKa = 9.08SSSDD99 pKa = 3.06VKK101 pKa = 11.23SYY103 pKa = 10.83IEE105 pKa = 4.56KK106 pKa = 10.78DD107 pKa = 2.66SDD109 pKa = 4.26YY110 pKa = 11.02ISNEE114 pKa = 2.8AWYY117 pKa = 10.73NVIDD121 pKa = 5.29DD122 pKa = 4.41VDD124 pKa = 3.62PHH126 pKa = 5.91YY127 pKa = 11.01LKK129 pKa = 10.8HH130 pKa = 5.59LTNHH134 pKa = 6.71AEE136 pKa = 3.93ALNAGSAEE144 pKa = 3.87AALAIIRR151 pKa = 11.84EE152 pKa = 4.23KK153 pKa = 10.87LPKK156 pKa = 10.46DD157 pKa = 4.77FIFQYY162 pKa = 11.21HH163 pKa = 5.47NLKK166 pKa = 10.81CNLEE170 pKa = 4.44RR171 pKa = 11.84IYY173 pKa = 10.07TPPMEE178 pKa = 4.77VYY180 pKa = 10.56VSPFSSSSFDD190 pKa = 3.41QVPEE194 pKa = 3.98EE195 pKa = 4.13LEE197 pKa = 3.58EE198 pKa = 3.9WAAEE202 pKa = 4.09NVVGDD207 pKa = 3.89AARR210 pKa = 11.84ANRR213 pKa = 11.84PISIVIEE220 pKa = 4.23GDD222 pKa = 3.2SRR224 pKa = 11.84TGKK227 pKa = 8.48TMWASNEE234 pKa = 3.22AWYY237 pKa = 10.85NVIDD241 pKa = 5.29DD242 pKa = 4.41VDD244 pKa = 3.61PHH246 pKa = 5.76YY247 pKa = 11.0LKK249 pKa = 10.83HH250 pKa = 6.2FSNEE254 pKa = 2.86AWYY257 pKa = 10.8NVIDD261 pKa = 5.29DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.22YY287 pKa = 10.09GKK289 pKa = 9.34PVGMYY294 pKa = 10.18

Molecular weight: 33.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5SQ21|V5SQ21_9GEMI Replication enhancer OS=Spinach yellow vein Sikar virus OX=1432565 GN=AC3 PE=3 SV=1
MM1 pKa = 6.94HH2 pKa = 7.56HH3 pKa = 6.49NRR5 pKa = 11.84PFLMNRR11 pKa = 11.84DD12 pKa = 3.73IITVQIQFNHH22 pKa = 6.03SLRR25 pKa = 11.84KK26 pKa = 9.53ALGIHH31 pKa = 5.89KK32 pKa = 9.94CFLAFRR38 pKa = 11.84IWMTSQPPIGRR49 pKa = 11.84FLRR52 pKa = 11.84VFKK55 pKa = 9.95TQVTNHH61 pKa = 5.14WNVLEE66 pKa = 4.09HH67 pKa = 6.28VVYY70 pKa = 10.85VDD72 pKa = 3.34QSSSIKK78 pKa = 10.0FNIWNVLEE86 pKa = 4.1HH87 pKa = 6.18VVYY90 pKa = 10.85VDD92 pKa = 3.34QSSSIKK98 pKa = 10.4FNIYY102 pKa = 10.21

Molecular weight: 12.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

995

75

294

165.8

19.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.126 ± 0.806

2.513 ± 0.844

4.724 ± 0.671

4.824 ± 0.52

4.623 ± 0.689

3.719 ± 0.377

4.322 ± 0.762

6.332 ± 0.739

5.226 ± 1.12

6.131 ± 1.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.915 ± 0.532

5.829 ± 0.831

6.734 ± 1.318

4.121 ± 0.652

5.829 ± 0.744

8.643 ± 1.707

5.226 ± 1.259

7.638 ± 1.145

1.407 ± 0.317

4.121 ± 0.875
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski