Sulfolobus spindle-shaped virus Lassen

Taxonomy: Viruses; Fuselloviridae; Alphafusellovirus; unclassified Alphafusellovirus

Average proteome isoelectric point is 7.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5WVM6|A0A1S5WVM6_9VIRU B298 OS=Sulfolobus spindle-shaped virus Lassen OX=1959008 PE=4 SV=1
MM1 pKa = 7.69RR2 pKa = 11.84KK3 pKa = 9.41SLLALLTLSLALLMLATPTGAISASQLGASQIVFTTNQFNFAGVSYY49 pKa = 9.78SNEE52 pKa = 3.83WISTFFHH59 pKa = 6.03GWNPIVYY66 pKa = 8.5DD67 pKa = 3.43VEE69 pKa = 4.97GIYY72 pKa = 10.46LGNISLGYY80 pKa = 10.06VNNPTGNGYY89 pKa = 9.86NEE91 pKa = 3.85IFIGIGLSAYY101 pKa = 8.65ATGTLGGAGTPGQIINNSVLAGLYY125 pKa = 9.89IVTTSSGYY133 pKa = 9.05YY134 pKa = 8.86YY135 pKa = 10.94YY136 pKa = 11.14VGAEE140 pKa = 3.93TLQNGNLIVAKK151 pKa = 9.99DD152 pKa = 3.63SSPITGSLPISLQIQLYY169 pKa = 10.75DD170 pKa = 4.54DD171 pKa = 4.62GNGTATAYY179 pKa = 7.63ITLYY183 pKa = 10.52YY184 pKa = 10.54SYY186 pKa = 11.65GLLTYY191 pKa = 10.21SFYY194 pKa = 11.43VPYY197 pKa = 10.23PFNPSNGYY205 pKa = 10.42ASYY208 pKa = 11.1SALTLIGAVLQLPYY222 pKa = 10.33ISGGLTSFDD231 pKa = 4.13FVYY234 pKa = 10.78QSNGQNYY241 pKa = 7.28EE242 pKa = 3.86WPNQIASGTQIMAGVYY258 pKa = 10.08SISGSVNEE266 pKa = 4.16LTQVSLYY273 pKa = 11.17NGFLNNGLWSYY284 pKa = 11.06IYY286 pKa = 10.54NFSNPYY292 pKa = 6.68PTTYY296 pKa = 11.45ALL298 pKa = 3.9

Molecular weight: 32.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5WVH6|A0A1S5WVH6_9VIRU ORF C82 OS=Sulfolobus spindle-shaped virus Lassen OX=1959008 PE=4 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84WGRR5 pKa = 11.84DD6 pKa = 3.0DD7 pKa = 3.99TYY9 pKa = 10.61TGKK12 pKa = 9.61IRR14 pKa = 11.84RR15 pKa = 11.84NSPRR19 pKa = 11.84FRR21 pKa = 11.84FNYY24 pKa = 9.17ADD26 pKa = 3.7YY27 pKa = 11.24VITIQKK33 pKa = 9.3AKK35 pKa = 9.92RR36 pKa = 11.84VEE38 pKa = 3.96RR39 pKa = 11.84EE40 pKa = 3.73VNIRR44 pKa = 11.84PEE46 pKa = 3.6KK47 pKa = 10.58HH48 pKa = 4.32KK49 pKa = 9.31TLRR52 pKa = 11.84VLSGHH57 pKa = 6.01ISYY60 pKa = 10.75RR61 pKa = 11.84SLSLEE66 pKa = 4.07SLCFLHH72 pKa = 6.86EE73 pKa = 4.4LSRR76 pKa = 11.84LLRR79 pKa = 11.84GVDD82 pKa = 3.53GG83 pKa = 4.47

Molecular weight: 9.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38

0

38

5342

49

814

140.6

16.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.755 ± 0.348

0.562 ± 0.21

3.575 ± 0.48

5.953 ± 1.032

4.53 ± 0.358

5.897 ± 0.637

1.273 ± 0.286

8.761 ± 0.511

7.881 ± 1.046

11.475 ± 0.562

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.134 ± 0.334

5.522 ± 0.517

3.557 ± 0.455

3.238 ± 0.308

3.37 ± 0.624

6.608 ± 0.815

6.72 ± 0.653

7.151 ± 0.473

1.367 ± 0.208

5.672 ± 0.512
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski