Amino acid dipepetide frequency for Leifsonia sp. Leaf336

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.62AlaAla: 20.62 ± 0.172
0.658AlaCys: 0.658 ± 0.024
8.069AlaAsp: 8.069 ± 0.091
8.045AlaGlu: 8.045 ± 0.105
4.23AlaPhe: 4.23 ± 0.068
12.482AlaGly: 12.482 ± 0.117
2.349AlaHis: 2.349 ± 0.045
5.972AlaIle: 5.972 ± 0.072
2.771AlaLys: 2.771 ± 0.057
14.221AlaLeu: 14.221 ± 0.128
2.507AlaMet: 2.507 ± 0.051
2.588AlaAsn: 2.588 ± 0.049
6.202AlaPro: 6.202 ± 0.09
3.794AlaGln: 3.794 ± 0.062
8.782AlaArg: 8.782 ± 0.111
7.688AlaSer: 7.688 ± 0.084
7.688AlaThr: 7.688 ± 0.095
12.107AlaVal: 12.107 ± 0.117
1.771AlaTrp: 1.771 ± 0.04
2.476AlaTyr: 2.476 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
0.613CysAla: 0.613 ± 0.024
0.042CysCys: 0.042 ± 0.007
0.281CysAsp: 0.281 ± 0.014
0.209CysGlu: 0.209 ± 0.013
0.164CysPhe: 0.164 ± 0.012
0.534CysGly: 0.534 ± 0.021
0.095CysHis: 0.095 ± 0.01
0.177CysIle: 0.177 ± 0.013
0.062CysLys: 0.062 ± 0.007
0.422CysLeu: 0.422 ± 0.017
0.065CysMet: 0.065 ± 0.007
0.099CysAsn: 0.099 ± 0.009
0.281CysPro: 0.281 ± 0.016
0.111CysGln: 0.111 ± 0.009
0.258CysArg: 0.258 ± 0.016
0.356CysSer: 0.356 ± 0.019
0.335CysThr: 0.335 ± 0.017
0.438CysVal: 0.438 ± 0.02
0.073CysTrp: 0.073 ± 0.009
0.103CysTyr: 0.103 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
8.434AspAla: 8.434 ± 0.097
0.237AspCys: 0.237 ± 0.014
4.25AspAsp: 4.25 ± 0.06
4.009AspGlu: 4.009 ± 0.065
1.809AspPhe: 1.809 ± 0.039
6.079AspGly: 6.079 ± 0.089
1.188AspHis: 1.188 ± 0.032
2.294AspIle: 2.294 ± 0.042
1.062AspLys: 1.062 ± 0.035
6.212AspLeu: 6.212 ± 0.065
0.699AspMet: 0.699 ± 0.022
1.041AspAsn: 1.041 ± 0.036
4.133AspPro: 4.133 ± 0.073
1.581AspGln: 1.581 ± 0.033
4.495AspArg: 4.495 ± 0.061
2.702AspSer: 2.702 ± 0.05
2.856AspThr: 2.856 ± 0.05
5.031AspVal: 5.031 ± 0.071
0.99AspTrp: 0.99 ± 0.034
1.31AspTyr: 1.31 ± 0.04
0.0AspXaa: 0.0 ± 0.0
Glu
7.295GluAla: 7.295 ± 0.094
0.193GluCys: 0.193 ± 0.012
2.37GluAsp: 2.37 ± 0.047
2.913GluGlu: 2.913 ± 0.052
1.669GluPhe: 1.669 ± 0.042
3.713GluGly: 3.713 ± 0.057
1.408GluHis: 1.408 ± 0.039
2.309GluIle: 2.309 ± 0.052
1.354GluLys: 1.354 ± 0.043
6.836GluLeu: 6.836 ± 0.081
0.744GluMet: 0.744 ± 0.025
1.153GluAsn: 1.153 ± 0.034
2.973GluPro: 2.973 ± 0.063
2.063GluGln: 2.063 ± 0.05
5.252GluArg: 5.252 ± 0.082
2.755GluSer: 2.755 ± 0.047
2.887GluThr: 2.887 ± 0.052
4.424GluVal: 4.424 ± 0.063
0.842GluTrp: 0.842 ± 0.026
1.06GluTyr: 1.06 ± 0.03
0.001GluXaa: 0.001 ± 0.001
Phe
4.573PheAla: 4.573 ± 0.066
0.187PheCys: 0.187 ± 0.013
2.377PheAsp: 2.377 ± 0.046
1.73PheGlu: 1.73 ± 0.038
1.18PhePhe: 1.18 ± 0.04
3.572PheGly: 3.572 ± 0.063
0.56PheHis: 0.56 ± 0.02
1.178PheIle: 1.178 ± 0.036
0.476PheLys: 0.476 ± 0.023
3.014PheLeu: 3.014 ± 0.058
0.412PheMet: 0.412 ± 0.022
0.696PheAsn: 0.696 ± 0.028
1.519PhePro: 1.519 ± 0.038
0.8PheGln: 0.8 ± 0.03
1.924PheArg: 1.924 ± 0.043
1.87PheSer: 1.87 ± 0.042
2.159PheThr: 2.159 ± 0.046
3.083PheVal: 3.083 ± 0.053
0.546PheTrp: 0.546 ± 0.024
0.74PheTyr: 0.74 ± 0.024
0.0PheXaa: 0.0 ± 0.0
Gly
10.936GlyAla: 10.936 ± 0.116
0.523GlyCys: 0.523 ± 0.021
5.106GlyAsp: 5.106 ± 0.069
4.648GlyGlu: 4.648 ± 0.067
3.342GlyPhe: 3.342 ± 0.051
7.87GlyGly: 7.87 ± 0.106
1.861GlyHis: 1.861 ± 0.047
4.687GlyIle: 4.687 ± 0.063
2.118GlyLys: 2.118 ± 0.048
9.105GlyLeu: 9.105 ± 0.1
1.928GlyMet: 1.928 ± 0.042
1.781GlyAsn: 1.781 ± 0.044
3.884GlyPro: 3.884 ± 0.051
2.45GlyGln: 2.45 ± 0.05
6.469GlyArg: 6.469 ± 0.064
5.697GlySer: 5.697 ± 0.078
5.634GlyThr: 5.634 ± 0.075
8.036GlyVal: 8.036 ± 0.084
1.788GlyTrp: 1.788 ± 0.043
2.423GlyTyr: 2.423 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
2.294HisAla: 2.294 ± 0.043
0.097HisCys: 0.097 ± 0.009
1.357HisAsp: 1.357 ± 0.032
1.129HisGlu: 1.129 ± 0.032
0.605HisPhe: 0.605 ± 0.022
1.959HisGly: 1.959 ± 0.048
0.506HisHis: 0.506 ± 0.023
0.721HisIle: 0.721 ± 0.023
0.281HisLys: 0.281 ± 0.014
1.908HisLeu: 1.908 ± 0.041
0.277HisMet: 0.277 ± 0.018
0.381HisAsn: 0.381 ± 0.019
1.515HisPro: 1.515 ± 0.038
0.493HisGln: 0.493 ± 0.019
1.463HisArg: 1.463 ± 0.037
0.995HisSer: 0.995 ± 0.025
0.992HisThr: 0.992 ± 0.029
1.601HisVal: 1.601 ± 0.039
0.31HisTrp: 0.31 ± 0.017
0.448HisTyr: 0.448 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
6.924IleAla: 6.924 ± 0.064
0.224IleCys: 0.224 ± 0.013
3.24IleAsp: 3.24 ± 0.05
2.528IleGlu: 2.528 ± 0.048
1.207IlePhe: 1.207 ± 0.039
4.672IleGly: 4.672 ± 0.065
0.737IleHis: 0.737 ± 0.025
1.729IleIle: 1.729 ± 0.047
0.78IleLys: 0.78 ± 0.027
3.722IleLeu: 3.722 ± 0.058
0.59IleMet: 0.59 ± 0.025
0.917IleAsn: 0.917 ± 0.027
2.419IlePro: 2.419 ± 0.046
1.03IleGln: 1.03 ± 0.026
2.578IleArg: 2.578 ± 0.048
2.22IleSer: 2.22 ± 0.049
2.657IleThr: 2.657 ± 0.052
4.959IleVal: 4.959 ± 0.066
0.554IleTrp: 0.554 ± 0.024
0.758IleTyr: 0.758 ± 0.028
0.0IleXaa: 0.0 ± 0.0
Lys
2.531LysAla: 2.531 ± 0.058
0.065LysCys: 0.065 ± 0.007
1.129LysAsp: 1.129 ± 0.039
0.963LysGlu: 0.963 ± 0.034
0.52LysPhe: 0.52 ± 0.023
1.564LysGly: 1.564 ± 0.038
0.449LysHis: 0.449 ± 0.02
0.897LysIle: 0.897 ± 0.031
0.782LysLys: 0.782 ± 0.032
2.001LysLeu: 2.001 ± 0.05
0.355LysMet: 0.355 ± 0.02
0.587LysAsn: 0.587 ± 0.026
1.262LysPro: 1.262 ± 0.038
0.771LysGln: 0.771 ± 0.025
1.526LysArg: 1.526 ± 0.034
1.189LysSer: 1.189 ± 0.041
1.337LysThr: 1.337 ± 0.04
1.675LysVal: 1.675 ± 0.048
0.249LysTrp: 0.249 ± 0.014
0.497LysTyr: 0.497 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
14.717LeuAla: 14.717 ± 0.142
0.503LeuCys: 0.503 ± 0.018
6.678LeuAsp: 6.678 ± 0.074
5.021LeuGlu: 5.021 ± 0.083
3.124LeuPhe: 3.124 ± 0.067
9.307LeuGly: 9.307 ± 0.104
2.008LeuHis: 2.008 ± 0.039
4.532LeuIle: 4.532 ± 0.068
1.829LeuLys: 1.829 ± 0.047
10.622LeuLeu: 10.622 ± 0.125
1.501LeuMet: 1.501 ± 0.037
1.933LeuAsn: 1.933 ± 0.04
5.82LeuPro: 5.82 ± 0.072
2.641LeuGln: 2.641 ± 0.047
7.409LeuArg: 7.409 ± 0.098
5.97LeuSer: 5.97 ± 0.076
6.633LeuThr: 6.633 ± 0.081
9.236LeuVal: 9.236 ± 0.102
1.319LeuTrp: 1.319 ± 0.032
1.81LeuTyr: 1.81 ± 0.039
0.002LeuXaa: 0.002 ± 0.001
Met
1.898MetAla: 1.898 ± 0.044
0.099MetCys: 0.099 ± 0.009
0.777MetAsp: 0.777 ± 0.029
0.63MetGlu: 0.63 ± 0.022
0.481MetPhe: 0.481 ± 0.021
1.146MetGly: 1.146 ± 0.035
0.295MetHis: 0.295 ± 0.014
0.807MetIle: 0.807 ± 0.026
0.402MetLys: 0.402 ± 0.018
1.752MetLeu: 1.752 ± 0.04
0.278MetMet: 0.278 ± 0.015
0.471MetAsn: 0.471 ± 0.021
0.984MetPro: 0.984 ± 0.027
0.518MetGln: 0.518 ± 0.018
1.283MetArg: 1.283 ± 0.031
1.337MetSer: 1.337 ± 0.031
1.627MetThr: 1.627 ± 0.034
1.247MetVal: 1.247 ± 0.031
0.176MetTrp: 0.176 ± 0.012
0.276MetTyr: 0.276 ± 0.017
0.001MetXaa: 0.001 ± 0.001
Asn
2.697AsnAla: 2.697 ± 0.046
0.124AsnCys: 0.124 ± 0.011
1.222AsnAsp: 1.222 ± 0.033
0.93AsnGlu: 0.93 ± 0.03
0.669AsnPhe: 0.669 ± 0.022
2.235AsnGly: 2.235 ± 0.054
0.367AsnHis: 0.367 ± 0.018
0.846AsnIle: 0.846 ± 0.026
0.46AsnLys: 0.46 ± 0.025
1.937AsnLeu: 1.937 ± 0.041
0.292AsnMet: 0.292 ± 0.015
0.516AsnAsn: 0.516 ± 0.024
1.672AsnPro: 1.672 ± 0.037
0.636AsnGln: 0.636 ± 0.024
1.309AsnArg: 1.309 ± 0.032
1.107AsnSer: 1.107 ± 0.033
1.244AsnThr: 1.244 ± 0.041
1.752AsnVal: 1.752 ± 0.039
0.33AsnTrp: 0.33 ± 0.017
0.537AsnTyr: 0.537 ± 0.024
0.0AsnXaa: 0.0 ± 0.0
Pro
7.378ProAla: 7.378 ± 0.096
0.163ProCys: 0.163 ± 0.013
3.744ProAsp: 3.744 ± 0.059
3.696ProGlu: 3.696 ± 0.065
1.842ProPhe: 1.842 ± 0.036
5.159ProGly: 5.159 ± 0.068
1.06ProHis: 1.06 ± 0.028
2.162ProIle: 2.162 ± 0.049
1.094ProLys: 1.094 ± 0.031
4.972ProLeu: 4.972 ± 0.07
0.824ProMet: 0.824 ± 0.027
1.149ProAsn: 1.149 ± 0.034
2.338ProPro: 2.338 ± 0.054
1.592ProGln: 1.592 ± 0.037
3.179ProArg: 3.179 ± 0.057
3.404ProSer: 3.404 ± 0.059
3.536ProThr: 3.536 ± 0.063
4.884ProVal: 4.884 ± 0.067
0.832ProTrp: 0.832 ± 0.031
1.093ProTyr: 1.093 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.488GlnAla: 3.488 ± 0.063
0.087GlnCys: 0.087 ± 0.009
1.226GlnAsp: 1.226 ± 0.031
1.311GlnGlu: 1.311 ± 0.036
0.912GlnPhe: 0.912 ± 0.025
1.984GlnGly: 1.984 ± 0.043
0.592GlnHis: 0.592 ± 0.023
1.248GlnIle: 1.248 ± 0.034
0.771GlnLys: 0.771 ± 0.029
3.448GlnLeu: 3.448 ± 0.057
0.432GlnMet: 0.432 ± 0.017
0.777GlnAsn: 0.777 ± 0.03
1.544GlnPro: 1.544 ± 0.041
1.291GlnGln: 1.291 ± 0.048
2.269GlnArg: 2.269 ± 0.045
1.584GlnSer: 1.584 ± 0.035
1.672GlnThr: 1.672 ± 0.041
2.339GlnVal: 2.339 ± 0.044
0.482GlnTrp: 0.482 ± 0.021
0.703GlnTyr: 0.703 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
8.574ArgAla: 8.574 ± 0.095
0.25ArgCys: 0.25 ± 0.014
4.115ArgAsp: 4.115 ± 0.059
4.258ArgGlu: 4.258 ± 0.061
2.461ArgPhe: 2.461 ± 0.044
5.211ArgGly: 5.211 ± 0.063
1.425ArgHis: 1.425 ± 0.036
3.578ArgIle: 3.578 ± 0.059
1.268ArgLys: 1.268 ± 0.035
7.407ArgLeu: 7.407 ± 0.099
1.584ArgMet: 1.584 ± 0.042
1.376ArgAsn: 1.376 ± 0.035
3.837ArgPro: 3.837 ± 0.06
1.946ArgGln: 1.946 ± 0.042
6.277ArgArg: 6.277 ± 0.098
4.27ArgSer: 4.27 ± 0.058
4.105ArgThr: 4.105 ± 0.066
6.044ArgVal: 6.044 ± 0.091
1.129ArgTrp: 1.129 ± 0.033
1.606ArgTyr: 1.606 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
7.552SerAla: 7.552 ± 0.087
0.25SerCys: 0.25 ± 0.014
3.292SerAsp: 3.292 ± 0.053
2.662SerGlu: 2.662 ± 0.043
1.946SerPhe: 1.946 ± 0.044
6.269SerGly: 6.269 ± 0.088
1.055SerHis: 1.055 ± 0.028
2.612SerIle: 2.612 ± 0.042
1.185SerLys: 1.185 ± 0.035
5.413SerLeu: 5.413 ± 0.066
1.088SerMet: 1.088 ± 0.031
1.237SerAsn: 1.237 ± 0.036
3.122SerPro: 3.122 ± 0.053
1.514SerGln: 1.514 ± 0.039
3.96SerArg: 3.96 ± 0.065
3.693SerSer: 3.693 ± 0.066
3.901SerThr: 3.901 ± 0.067
4.868SerVal: 4.868 ± 0.068
1.025SerTrp: 1.025 ± 0.03
1.289SerTyr: 1.289 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
8.144ThrAla: 8.144 ± 0.092
0.283ThrCys: 0.283 ± 0.016
3.476ThrAsp: 3.476 ± 0.064
2.892ThrGlu: 2.892 ± 0.054
1.968ThrPhe: 1.968 ± 0.046
6.036ThrGly: 6.036 ± 0.08
1.05ThrHis: 1.05 ± 0.03
3.049ThrIle: 3.049 ± 0.055
1.271ThrLys: 1.271 ± 0.035
6.041ThrLeu: 6.041 ± 0.066
0.962ThrMet: 0.962 ± 0.025
1.288ThrAsn: 1.288 ± 0.038
3.934ThrPro: 3.934 ± 0.064
1.535ThrGln: 1.535 ± 0.042
3.6ThrArg: 3.6 ± 0.063
3.687ThrSer: 3.687 ± 0.073
4.169ThrThr: 4.169 ± 0.083
6.143ThrVal: 6.143 ± 0.086
0.915ThrTrp: 0.915 ± 0.03
1.221ThrTyr: 1.221 ± 0.04
0.001ThrXaa: 0.001 ± 0.001
Val
12.236ValAla: 12.236 ± 0.114
0.504ValCys: 0.504 ± 0.021
5.516ValAsp: 5.516 ± 0.069
4.858ValGlu: 4.858 ± 0.07
3.037ValPhe: 3.037 ± 0.056
7.412ValGly: 7.412 ± 0.078
1.649ValHis: 1.649 ± 0.035
4.198ValIle: 4.198 ± 0.066
1.669ValLys: 1.669 ± 0.042
9.43ValLeu: 9.43 ± 0.118
1.355ValMet: 1.355 ± 0.037
1.912ValAsn: 1.912 ± 0.046
4.813ValPro: 4.813 ± 0.062
2.196ValGln: 2.196 ± 0.041
5.913ValArg: 5.913 ± 0.079
5.127ValSer: 5.127 ± 0.058
5.894ValThr: 5.894 ± 0.081
9.238ValVal: 9.238 ± 0.104
1.169ValTrp: 1.169 ± 0.035
1.743ValTyr: 1.743 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
1.59TrpAla: 1.59 ± 0.037
0.099TrpCys: 0.099 ± 0.01
0.762TrpAsp: 0.762 ± 0.029
0.669TrpGlu: 0.669 ± 0.025
0.634TrpPhe: 0.634 ± 0.025
1.084TrpGly: 1.084 ± 0.029
0.349TrpHis: 0.349 ± 0.018
0.749TrpIle: 0.749 ± 0.024
0.353TrpLys: 0.353 ± 0.017
1.836TrpLeu: 1.836 ± 0.044
0.334TrpMet: 0.334 ± 0.018
0.466TrpAsn: 0.466 ± 0.018
0.731TrpPro: 0.731 ± 0.028
0.552TrpGln: 0.552 ± 0.021
1.176TrpArg: 1.176 ± 0.033
1.062TrpSer: 1.062 ± 0.028
0.993TrpThr: 0.993 ± 0.028
1.102TrpVal: 1.102 ± 0.034
0.382TrpTrp: 0.382 ± 0.022
0.316TrpTyr: 0.316 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.524TyrAla: 2.524 ± 0.044
0.13TyrCys: 0.13 ± 0.01
1.428TyrAsp: 1.428 ± 0.049
1.111TyrGlu: 1.111 ± 0.031
0.797TyrPhe: 0.797 ± 0.029
1.999TyrGly: 1.999 ± 0.047
0.326TyrHis: 0.326 ± 0.016
0.7TyrIle: 0.7 ± 0.025
0.362TyrLys: 0.362 ± 0.019
2.314TyrLeu: 2.314 ± 0.046
0.256TyrMet: 0.256 ± 0.013
0.511TyrAsn: 0.511 ± 0.023
1.115TyrPro: 1.115 ± 0.034
0.65TyrGln: 0.65 ± 0.024
1.601TyrArg: 1.601 ± 0.041
1.219TyrSer: 1.219 ± 0.033
1.345TyrThr: 1.345 ± 0.043
1.665TyrVal: 1.665 ± 0.041
0.359TyrTrp: 0.359 ± 0.019
0.538TyrTyr: 0.538 ± 0.025
0.001TyrXaa: 0.001 ± 0.001
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.001XaaPhe: 0.001 ± 0.001
0.001XaaGly: 0.001 ± 0.001
0.0XaaHis: 0.0 ± 0.0
0.001XaaIle: 0.001 ± 0.001
0.0XaaLys: 0.0 ± 0.0
0.001XaaLeu: 0.001 ± 0.001
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.001XaaThr: 0.001 ± 0.001
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.001XaaTyr: 0.001 ± 0.001
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3857 proteins (1226354 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski