Streptomyces phage Janus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Arequatrovirus; unclassified Arequatrovirus

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411CQ77|A0A411CQ77_9CAUD Exonuclease OS=Streptomyces phage Janus OX=2510525 GN=32 PE=4 SV=1
MM1 pKa = 7.75ALNDD5 pKa = 3.53NATLVIGSGNYY16 pKa = 7.76LTAPTGTDD24 pKa = 2.86IPADD28 pKa = 3.82LLVPVAPWEE37 pKa = 4.41AVGHH41 pKa = 5.85TSLEE45 pKa = 4.62DD46 pKa = 3.05IFSITSEE53 pKa = 4.05GGEE56 pKa = 3.88ATVIGSLQNKK66 pKa = 7.32SLRR69 pKa = 11.84TKK71 pKa = 10.19YY72 pKa = 10.01SARR75 pKa = 11.84TEE77 pKa = 4.2TMTFTLQQFDD87 pKa = 3.96VKK89 pKa = 10.73GLKK92 pKa = 9.98LYY94 pKa = 10.32YY95 pKa = 10.43GSNAPVLADD104 pKa = 3.56GSVGVPADD112 pKa = 4.07PVPTTCAFLAVFVDD126 pKa = 4.43GEE128 pKa = 4.32NHH130 pKa = 6.2FAFYY134 pKa = 10.7APKK137 pKa = 10.65AEE139 pKa = 4.57IYY141 pKa = 10.35RR142 pKa = 11.84ADD144 pKa = 3.79DD145 pKa = 3.35VSFGDD150 pKa = 3.87TEE152 pKa = 4.44SLAGLPLGVKK162 pKa = 10.04PMAYY166 pKa = 9.89GANSWTYY173 pKa = 11.18AITPLGGIVATGATAGTPGAFTPDD197 pKa = 3.25GATPPFALADD207 pKa = 4.08LASVIATPTTAWTTGQHH224 pKa = 5.0VVLGDD229 pKa = 3.54GSNAYY234 pKa = 8.97WDD236 pKa = 3.8GDD238 pKa = 3.53SWNTGIAAA246 pKa = 4.5

Molecular weight: 25.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411CQI3|A0A411CQI3_9CAUD Uncharacterized protein OS=Streptomyces phage Janus OX=2510525 GN=69 PE=4 SV=1
MM1 pKa = 7.45SCNKK5 pKa = 9.24GVAWRR10 pKa = 11.84PCQCGLKK17 pKa = 10.28RR18 pKa = 11.84GFTSEE23 pKa = 3.92RR24 pKa = 11.84DD25 pKa = 3.12ADD27 pKa = 3.89KK28 pKa = 11.44ALGRR32 pKa = 11.84AQAKK36 pKa = 9.67RR37 pKa = 11.84NRR39 pKa = 11.84RR40 pKa = 11.84SDD42 pKa = 3.78AIGTRR47 pKa = 11.84RR48 pKa = 11.84GQHH51 pKa = 4.84IEE53 pKa = 3.34RR54 pKa = 11.84RR55 pKa = 11.84YY56 pKa = 8.94YY57 pKa = 9.61LCEE60 pKa = 3.74YY61 pKa = 10.92GLFHH65 pKa = 6.76LTSEE69 pKa = 4.33NRR71 pKa = 11.84SSYY74 pKa = 9.33ATRR77 pKa = 11.84TNKK80 pKa = 9.47MEE82 pKa = 4.01VAKK85 pKa = 10.87

Molecular weight: 9.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

15315

36

1065

196.3

21.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.29 ± 0.334

0.725 ± 0.102

6.601 ± 0.207

7.15 ± 0.476

2.873 ± 0.191

8.364 ± 0.398

2.037 ± 0.188

4.59 ± 0.31

4.427 ± 0.294

8.39 ± 0.382

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.155 ± 0.126

2.847 ± 0.157

4.825 ± 0.217

3.474 ± 0.136

6.706 ± 0.434

5.759 ± 0.315

6.164 ± 0.288

7.026 ± 0.258

1.796 ± 0.103

2.801 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski