Amino acid dipepetide frequency for Streptomyces phage Janus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.668AlaAla: 12.668 ± 1.068
0.588AlaCys: 0.588 ± 0.195
8.554AlaAsp: 8.554 ± 0.911
8.881AlaGlu: 8.881 ± 0.977
2.677AlaPhe: 2.677 ± 0.383
8.489AlaGly: 8.489 ± 0.807
2.155AlaHis: 2.155 ± 0.383
4.44AlaIle: 4.44 ± 0.546
5.877AlaLys: 5.877 ± 0.754
11.754AlaLeu: 11.754 ± 0.992
2.873AlaMet: 2.873 ± 0.478
3.396AlaAsn: 3.396 ± 0.518
4.506AlaPro: 4.506 ± 0.544
3.461AlaGln: 3.461 ± 0.491
6.269AlaArg: 6.269 ± 0.639
5.746AlaSer: 5.746 ± 0.639
7.118AlaThr: 7.118 ± 0.685
8.489AlaVal: 8.489 ± 0.701
2.09AlaTrp: 2.09 ± 0.335
2.808AlaTyr: 2.808 ± 0.445
0.0AlaXaa: 0.0 ± 0.0
Cys
0.588CysAla: 0.588 ± 0.182
0.0CysCys: 0.0 ± 0.0
0.522CysAsp: 0.522 ± 0.165
0.457CysGlu: 0.457 ± 0.175
0.392CysPhe: 0.392 ± 0.207
0.653CysGly: 0.653 ± 0.191
0.261CysHis: 0.261 ± 0.123
0.392CysIle: 0.392 ± 0.165
0.522CysLys: 0.522 ± 0.251
0.261CysLeu: 0.261 ± 0.119
0.0CysMet: 0.0 ± 0.0
0.392CysAsn: 0.392 ± 0.164
0.588CysPro: 0.588 ± 0.269
0.326CysGln: 0.326 ± 0.15
0.522CysArg: 0.522 ± 0.227
0.588CysSer: 0.588 ± 0.195
0.326CysThr: 0.326 ± 0.179
0.196CysVal: 0.196 ± 0.156
0.065CysTrp: 0.065 ± 0.061
0.196CysTyr: 0.196 ± 0.121
0.0CysXaa: 0.0 ± 0.0
Asp
7.509AspAla: 7.509 ± 0.711
0.457AspCys: 0.457 ± 0.216
4.506AspAsp: 4.506 ± 0.586
5.942AspGlu: 5.942 ± 0.877
1.698AspPhe: 1.698 ± 0.324
6.465AspGly: 6.465 ± 0.611
1.371AspHis: 1.371 ± 0.318
3.2AspIle: 3.2 ± 0.474
2.09AspLys: 2.09 ± 0.392
5.224AspLeu: 5.224 ± 0.77
2.09AspMet: 2.09 ± 0.284
1.306AspAsn: 1.306 ± 0.271
4.049AspPro: 4.049 ± 0.502
1.894AspGln: 1.894 ± 0.32
4.375AspArg: 4.375 ± 0.538
3.526AspSer: 3.526 ± 0.581
2.938AspThr: 2.938 ± 0.428
4.44AspVal: 4.44 ± 0.493
1.567AspTrp: 1.567 ± 0.342
1.371AspTyr: 1.371 ± 0.286
0.0AspXaa: 0.0 ± 0.0
Glu
9.011GluAla: 9.011 ± 0.907
0.522GluCys: 0.522 ± 0.205
4.44GluAsp: 4.44 ± 0.636
6.138GluGlu: 6.138 ± 0.972
1.763GluPhe: 1.763 ± 0.34
5.942GluGly: 5.942 ± 0.622
1.828GluHis: 1.828 ± 0.43
3.134GluIle: 3.134 ± 0.492
2.285GluLys: 2.285 ± 0.429
8.424GluLeu: 8.424 ± 0.701
0.979GluMet: 0.979 ± 0.225
1.241GluAsn: 1.241 ± 0.291
3.004GluPro: 3.004 ± 0.548
2.873GluGln: 2.873 ± 0.478
4.702GluArg: 4.702 ± 0.631
3.461GluSer: 3.461 ± 0.586
3.526GluThr: 3.526 ± 0.579
4.571GluVal: 4.571 ± 0.687
1.437GluTrp: 1.437 ± 0.313
2.22GluTyr: 2.22 ± 0.423
0.0GluXaa: 0.0 ± 0.0
Phe
3.004PheAla: 3.004 ± 0.523
0.196PheCys: 0.196 ± 0.126
2.155PheAsp: 2.155 ± 0.372
2.416PheGlu: 2.416 ± 0.386
0.653PhePhe: 0.653 ± 0.22
3.004PheGly: 3.004 ± 0.53
0.588PheHis: 0.588 ± 0.231
1.11PheIle: 1.11 ± 0.282
0.914PheLys: 0.914 ± 0.231
1.828PheLeu: 1.828 ± 0.431
0.457PheMet: 0.457 ± 0.243
0.979PheAsn: 0.979 ± 0.219
1.437PhePro: 1.437 ± 0.338
0.979PheGln: 0.979 ± 0.266
2.285PheArg: 2.285 ± 0.359
1.371PheSer: 1.371 ± 0.271
1.828PheThr: 1.828 ± 0.285
2.285PheVal: 2.285 ± 0.419
0.522PheTrp: 0.522 ± 0.158
0.718PheTyr: 0.718 ± 0.208
0.0PheXaa: 0.0 ± 0.0
Gly
7.64GlyAla: 7.64 ± 0.95
0.065GlyCys: 0.065 ± 0.069
5.028GlyAsp: 5.028 ± 0.502
5.616GlyGlu: 5.616 ± 0.556
3.004GlyPhe: 3.004 ± 0.411
7.509GlyGly: 7.509 ± 1.13
2.547GlyHis: 2.547 ± 0.397
4.114GlyIle: 4.114 ± 0.851
4.44GlyLys: 4.44 ± 0.849
6.203GlyLeu: 6.203 ± 0.765
1.567GlyMet: 1.567 ± 0.298
2.416GlyAsn: 2.416 ± 0.375
3.722GlyPro: 3.722 ± 0.443
3.526GlyGln: 3.526 ± 0.44
4.963GlyArg: 4.963 ± 0.599
4.506GlySer: 4.506 ± 0.712
5.485GlyThr: 5.485 ± 0.863
6.269GlyVal: 6.269 ± 0.712
2.547GlyTrp: 2.547 ± 0.386
2.481GlyTyr: 2.481 ± 0.407
0.0GlyXaa: 0.0 ± 0.0
His
1.632HisAla: 1.632 ± 0.39
0.196HisCys: 0.196 ± 0.13
1.306HisAsp: 1.306 ± 0.302
1.306HisGlu: 1.306 ± 0.359
0.979HisPhe: 0.979 ± 0.286
1.828HisGly: 1.828 ± 0.348
0.979HisHis: 0.979 ± 0.251
0.849HisIle: 0.849 ± 0.244
0.457HisLys: 0.457 ± 0.157
2.09HisLeu: 2.09 ± 0.374
0.196HisMet: 0.196 ± 0.127
0.457HisAsn: 0.457 ± 0.201
1.241HisPro: 1.241 ± 0.351
0.718HisGln: 0.718 ± 0.213
1.371HisArg: 1.371 ± 0.37
1.175HisSer: 1.175 ± 0.386
1.11HisThr: 1.11 ± 0.278
1.306HisVal: 1.306 ± 0.346
0.588HisTrp: 0.588 ± 0.238
0.588HisTyr: 0.588 ± 0.21
0.0HisXaa: 0.0 ± 0.0
Ile
4.963IleAla: 4.963 ± 0.527
0.326IleCys: 0.326 ± 0.165
4.702IleAsp: 4.702 ± 0.627
3.722IleGlu: 3.722 ± 0.491
1.371IlePhe: 1.371 ± 0.35
3.918IleGly: 3.918 ± 0.723
0.718IleHis: 0.718 ± 0.185
1.632IleIle: 1.632 ± 0.454
1.894IleLys: 1.894 ± 0.528
2.677IleLeu: 2.677 ± 0.464
0.457IleMet: 0.457 ± 0.319
1.632IleAsn: 1.632 ± 0.406
2.416IlePro: 2.416 ± 0.408
1.698IleGln: 1.698 ± 0.334
3.069IleArg: 3.069 ± 0.458
2.024IleSer: 2.024 ± 0.495
3.591IleThr: 3.591 ± 0.447
3.591IleVal: 3.591 ± 0.475
0.392IleTrp: 0.392 ± 0.185
1.11IleTyr: 1.11 ± 0.236
0.0IleXaa: 0.0 ± 0.0
Lys
5.028LysAla: 5.028 ± 0.634
0.326LysCys: 0.326 ± 0.131
2.873LysAsp: 2.873 ± 0.531
2.285LysGlu: 2.285 ± 0.427
0.914LysPhe: 0.914 ± 0.259
4.31LysGly: 4.31 ± 0.643
0.457LysHis: 0.457 ± 0.196
1.959LysIle: 1.959 ± 0.32
3.069LysLys: 3.069 ± 0.646
3.787LysLeu: 3.787 ± 0.697
0.784LysMet: 0.784 ± 0.224
0.979LysAsn: 0.979 ± 0.271
2.808LysPro: 2.808 ± 0.603
1.828LysGln: 1.828 ± 0.272
3.461LysArg: 3.461 ± 0.629
2.351LysSer: 2.351 ± 0.414
2.22LysThr: 2.22 ± 0.37
2.873LysVal: 2.873 ± 0.415
0.261LysTrp: 0.261 ± 0.127
1.698LysTyr: 1.698 ± 0.351
0.0LysXaa: 0.0 ± 0.0
Leu
11.362LeuAla: 11.362 ± 0.879
0.653LeuCys: 0.653 ± 0.213
6.269LeuAsp: 6.269 ± 0.612
4.702LeuGlu: 4.702 ± 0.702
1.632LeuPhe: 1.632 ± 0.256
6.203LeuGly: 6.203 ± 0.676
0.979LeuHis: 0.979 ± 0.211
4.767LeuIle: 4.767 ± 0.71
3.2LeuLys: 3.2 ± 0.622
6.595LeuLeu: 6.595 ± 0.658
1.567LeuMet: 1.567 ± 0.425
3.265LeuAsn: 3.265 ± 0.461
4.31LeuPro: 4.31 ± 0.525
3.134LeuGln: 3.134 ± 0.447
6.334LeuArg: 6.334 ± 0.929
5.289LeuSer: 5.289 ± 0.503
5.355LeuThr: 5.355 ± 0.629
5.224LeuVal: 5.224 ± 0.62
1.045LeuTrp: 1.045 ± 0.279
2.024LeuTyr: 2.024 ± 0.33
0.0LeuXaa: 0.0 ± 0.0
Met
3.722MetAla: 3.722 ± 0.446
0.131MetCys: 0.131 ± 0.106
0.849MetAsp: 0.849 ± 0.21
1.306MetGlu: 1.306 ± 0.322
0.653MetPhe: 0.653 ± 0.227
1.437MetGly: 1.437 ± 0.312
0.522MetHis: 0.522 ± 0.184
0.914MetIle: 0.914 ± 0.224
0.849MetLys: 0.849 ± 0.237
1.371MetLeu: 1.371 ± 0.381
0.457MetMet: 0.457 ± 0.172
0.522MetAsn: 0.522 ± 0.147
1.241MetPro: 1.241 ± 0.3
0.718MetGln: 0.718 ± 0.193
1.11MetArg: 1.11 ± 0.323
2.285MetSer: 2.285 ± 0.339
1.763MetThr: 1.763 ± 0.329
0.914MetVal: 0.914 ± 0.245
0.261MetTrp: 0.261 ± 0.125
0.522MetTyr: 0.522 ± 0.176
0.0MetXaa: 0.0 ± 0.0
Asn
2.808AsnAla: 2.808 ± 0.47
0.522AsnCys: 0.522 ± 0.224
1.502AsnAsp: 1.502 ± 0.29
1.502AsnGlu: 1.502 ± 0.338
1.241AsnPhe: 1.241 ± 0.272
3.069AsnGly: 3.069 ± 0.459
0.653AsnHis: 0.653 ± 0.212
1.567AsnIle: 1.567 ± 0.233
0.914AsnLys: 0.914 ± 0.258
2.22AsnLeu: 2.22 ± 0.559
0.457AsnMet: 0.457 ± 0.179
1.11AsnAsn: 1.11 ± 0.303
1.632AsnPro: 1.632 ± 0.322
1.11AsnGln: 1.11 ± 0.251
1.959AsnArg: 1.959 ± 0.394
1.437AsnSer: 1.437 ± 0.35
1.763AsnThr: 1.763 ± 0.325
1.698AsnVal: 1.698 ± 0.344
0.392AsnTrp: 0.392 ± 0.172
0.914AsnTyr: 0.914 ± 0.219
0.0AsnXaa: 0.0 ± 0.0
Pro
5.746ProAla: 5.746 ± 0.656
0.588ProCys: 0.588 ± 0.205
3.2ProAsp: 3.2 ± 0.449
3.396ProGlu: 3.396 ± 0.493
0.979ProPhe: 0.979 ± 0.273
4.571ProGly: 4.571 ± 0.516
0.522ProHis: 0.522 ± 0.157
1.894ProIle: 1.894 ± 0.379
2.677ProLys: 2.677 ± 0.56
3.265ProLeu: 3.265 ± 0.604
1.175ProMet: 1.175 ± 0.251
1.371ProAsn: 1.371 ± 0.263
2.285ProPro: 2.285 ± 0.406
1.959ProGln: 1.959 ± 0.327
2.677ProArg: 2.677 ± 0.459
3.069ProSer: 3.069 ± 0.631
3.461ProThr: 3.461 ± 0.503
3.657ProVal: 3.657 ± 0.457
0.849ProTrp: 0.849 ± 0.205
0.914ProTyr: 0.914 ± 0.249
0.0ProXaa: 0.0 ± 0.0
Gln
4.767GlnAla: 4.767 ± 0.484
0.326GlnCys: 0.326 ± 0.158
1.175GlnAsp: 1.175 ± 0.298
2.938GlnGlu: 2.938 ± 0.59
0.784GlnPhe: 0.784 ± 0.215
2.22GlnGly: 2.22 ± 0.491
0.522GlnHis: 0.522 ± 0.206
2.155GlnIle: 2.155 ± 0.361
1.632GlnLys: 1.632 ± 0.411
3.004GlnLeu: 3.004 ± 0.471
1.567GlnMet: 1.567 ± 0.346
0.849GlnAsn: 0.849 ± 0.237
1.175GlnPro: 1.175 ± 0.27
0.914GlnGln: 0.914 ± 0.268
2.351GlnArg: 2.351 ± 0.385
1.959GlnSer: 1.959 ± 0.362
1.894GlnThr: 1.894 ± 0.364
2.873GlnVal: 2.873 ± 0.381
0.784GlnTrp: 0.784 ± 0.24
0.849GlnTyr: 0.849 ± 0.203
0.0GlnXaa: 0.0 ± 0.0
Arg
6.791ArgAla: 6.791 ± 0.676
0.784ArgCys: 0.784 ± 0.229
4.31ArgAsp: 4.31 ± 0.553
4.963ArgGlu: 4.963 ± 0.732
3.004ArgPhe: 3.004 ± 0.369
3.983ArgGly: 3.983 ± 0.523
1.502ArgHis: 1.502 ± 0.357
3.069ArgIle: 3.069 ± 0.434
3.787ArgLys: 3.787 ± 0.565
5.485ArgLeu: 5.485 ± 0.627
1.763ArgMet: 1.763 ± 0.373
1.698ArgAsn: 1.698 ± 0.381
2.743ArgPro: 2.743 ± 0.482
2.808ArgGln: 2.808 ± 0.479
5.355ArgArg: 5.355 ± 0.932
3.069ArgSer: 3.069 ± 0.466
4.44ArgThr: 4.44 ± 0.582
4.375ArgVal: 4.375 ± 0.465
1.11ArgTrp: 1.11 ± 0.294
2.024ArgTyr: 2.024 ± 0.324
0.0ArgXaa: 0.0 ± 0.0
Ser
5.355SerAla: 5.355 ± 0.619
0.196SerCys: 0.196 ± 0.118
3.526SerAsp: 3.526 ± 0.51
3.657SerGlu: 3.657 ± 0.511
1.894SerPhe: 1.894 ± 0.26
5.616SerGly: 5.616 ± 0.722
1.175SerHis: 1.175 ± 0.447
2.677SerIle: 2.677 ± 0.332
2.155SerLys: 2.155 ± 0.389
5.159SerLeu: 5.159 ± 0.563
1.11SerMet: 1.11 ± 0.259
1.437SerAsn: 1.437 ± 0.329
2.155SerPro: 2.155 ± 0.384
1.632SerGln: 1.632 ± 0.295
4.114SerArg: 4.114 ± 0.527
4.049SerSer: 4.049 ± 0.799
3.722SerThr: 3.722 ± 0.665
4.179SerVal: 4.179 ± 0.548
1.175SerTrp: 1.175 ± 0.235
2.612SerTyr: 2.612 ± 0.426
0.0SerXaa: 0.0 ± 0.0
Thr
6.203ThrAla: 6.203 ± 0.56
0.653ThrCys: 0.653 ± 0.212
3.396ThrAsp: 3.396 ± 0.405
4.571ThrGlu: 4.571 ± 0.555
2.22ThrPhe: 2.22 ± 0.454
5.159ThrGly: 5.159 ± 0.812
0.979ThrHis: 0.979 ± 0.275
3.526ThrIle: 3.526 ± 0.606
1.959ThrLys: 1.959 ± 0.404
4.702ThrLeu: 4.702 ± 0.602
1.241ThrMet: 1.241 ± 0.286
1.567ThrAsn: 1.567 ± 0.362
3.526ThrPro: 3.526 ± 0.629
1.175ThrGln: 1.175 ± 0.299
3.853ThrArg: 3.853 ± 0.523
4.832ThrSer: 4.832 ± 0.71
3.722ThrThr: 3.722 ± 0.496
5.355ThrVal: 5.355 ± 0.556
0.979ThrTrp: 0.979 ± 0.27
2.024ThrTyr: 2.024 ± 0.451
0.0ThrXaa: 0.0 ± 0.0
Val
8.032ValAla: 8.032 ± 0.68
0.392ValCys: 0.392 ± 0.185
3.853ValAsp: 3.853 ± 0.468
4.506ValGlu: 4.506 ± 0.581
1.828ValPhe: 1.828 ± 0.443
4.767ValGly: 4.767 ± 0.568
1.698ValHis: 1.698 ± 0.321
3.591ValIle: 3.591 ± 0.45
3.265ValLys: 3.265 ± 0.554
6.073ValLeu: 6.073 ± 0.667
1.632ValMet: 1.632 ± 0.321
2.22ValAsn: 2.22 ± 0.534
3.657ValPro: 3.657 ± 0.474
2.938ValGln: 2.938 ± 0.422
4.571ValArg: 4.571 ± 0.69
4.375ValSer: 4.375 ± 0.512
4.767ValThr: 4.767 ± 0.626
5.159ValVal: 5.159 ± 0.611
1.241ValTrp: 1.241 ± 0.268
1.698ValTyr: 1.698 ± 0.315
0.0ValXaa: 0.0 ± 0.0
Trp
2.285TrpAla: 2.285 ± 0.351
0.131TrpCys: 0.131 ± 0.085
1.567TrpAsp: 1.567 ± 0.324
1.175TrpGlu: 1.175 ± 0.285
0.457TrpPhe: 0.457 ± 0.183
1.371TrpGly: 1.371 ± 0.38
0.326TrpHis: 0.326 ± 0.151
0.261TrpIle: 0.261 ± 0.126
1.241TrpLys: 1.241 ± 0.237
1.502TrpLeu: 1.502 ± 0.313
0.392TrpMet: 0.392 ± 0.152
1.045TrpAsn: 1.045 ± 0.324
0.588TrpPro: 0.588 ± 0.199
0.457TrpGln: 0.457 ± 0.264
1.502TrpArg: 1.502 ± 0.286
0.849TrpSer: 0.849 ± 0.24
1.241TrpThr: 1.241 ± 0.31
1.11TrpVal: 1.11 ± 0.252
0.131TrpTrp: 0.131 ± 0.088
0.326TrpTyr: 0.326 ± 0.134
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.787TyrAla: 3.787 ± 0.492
0.196TyrCys: 0.196 ± 0.102
2.285TyrAsp: 2.285 ± 0.423
2.024TyrGlu: 2.024 ± 0.364
0.588TyrPhe: 0.588 ± 0.214
3.134TyrGly: 3.134 ± 0.595
0.588TyrHis: 0.588 ± 0.202
0.653TyrIle: 0.653 ± 0.181
1.045TyrLys: 1.045 ± 0.298
2.285TyrLeu: 2.285 ± 0.452
0.718TyrMet: 0.718 ± 0.19
0.588TyrAsn: 0.588 ± 0.207
1.175TyrPro: 1.175 ± 0.243
0.588TyrGln: 0.588 ± 0.181
2.22TyrArg: 2.22 ± 0.452
1.632TyrSer: 1.632 ± 0.395
1.371TyrThr: 1.371 ± 0.292
1.698TyrVal: 1.698 ± 0.382
0.522TyrTrp: 0.522 ± 0.185
0.914TyrTyr: 0.914 ± 0.249
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 78 proteins (15315 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski