Streptococcus satellite phage Javan598
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZWI9|A0A4D5ZWI9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan598 OX=2558767 GN=JavanS598_0010 PE=4 SV=1
MM1 pKa = 7.73 KK2 pKa = 10.47 DD3 pKa = 3.07 KK4 pKa = 11.24 TNDD7 pKa = 3.05 ILVDD11 pKa = 3.67 YY12 pKa = 10.4 EE13 pKa = 4.54 GLCGQLTNICEE24 pKa = 4.36 VLDD27 pKa = 4.09 LATAGHH33 pKa = 6.78 DD34 pKa = 3.44 QTTTSAIVNTVLQALEE50 pKa = 4.75 KK51 pKa = 10.37 IIADD55 pKa = 3.56 HH56 pKa = 7.36 KK57 pKa = 10.8 EE58 pKa = 3.71 LTNGYY63 pKa = 9.73 RR64 pKa = 11.84 EE65 pKa = 4.26 DD66 pKa = 4.22 LKK68 pKa = 11.46 HH69 pKa = 5.74 EE70 pKa = 4.19
Molecular weight: 7.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.539
IPC2_protein 4.622
IPC_protein 4.482
Toseland 4.329
ProMoST 4.507
Dawson 4.444
Bjellqvist 4.673
Wikipedia 4.355
Rodwell 4.329
Grimsley 4.24
Solomon 4.444
Lehninger 4.393
Nozaki 4.571
DTASelect 4.749
Thurlkill 4.355
EMBOSS 4.368
Sillero 4.609
Patrickios 3.63
IPC_peptide 4.444
IPC2_peptide 4.596
IPC2.peptide.svr19 4.541
Protein with the highest isoelectric point:
>tr|A0A4D5ZWJ8|A0A4D5ZWJ8_9VIRU Integrase/recombinase OS=Streptococcus satellite phage Javan598 OX=2558767 GN=JavanS598_0020 PE=3 SV=1
MM1 pKa = 7.49 SKK3 pKa = 11.04 YY4 pKa = 8.38 NTHH7 pKa = 7.3 PATSLGNWKK16 pKa = 8.81 MKK18 pKa = 10.21 NVHH21 pKa = 5.33 TSKK24 pKa = 10.5 EE25 pKa = 4.21 KK26 pKa = 10.11 NRR28 pKa = 11.84 HH29 pKa = 4.94 FVTDD33 pKa = 3.61 DD34 pKa = 3.11 QEE36 pKa = 4.12 QRR38 pKa = 11.84 RR39 pKa = 11.84 LANKK43 pKa = 9.21 LRR45 pKa = 11.84 KK46 pKa = 9.4 KK47 pKa = 9.81 RR48 pKa = 11.84 GKK50 pKa = 10.27 RR51 pKa = 11.84 KK52 pKa = 9.73
Molecular weight: 6.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.249
IPC2_protein 10.043
IPC_protein 10.906
Toseland 11.359
ProMoST 11.316
Dawson 11.389
Bjellqvist 11.14
Wikipedia 11.652
Rodwell 11.725
Grimsley 11.418
Solomon 11.637
Lehninger 11.579
Nozaki 11.33
DTASelect 11.14
Thurlkill 11.33
EMBOSS 11.784
Sillero 11.345
Patrickios 11.462
IPC_peptide 11.637
IPC2_peptide 10.087
IPC2.peptide.svr19 8.563
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
20
0
20
3183
49
535
159.2
18.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.692 ± 0.381
0.346 ± 0.155
5.624 ± 0.36
8.357 ± 0.713
3.676 ± 0.35
4.775 ± 0.455
1.948 ± 0.28
6.535 ± 0.459
9.739 ± 0.49
10.179 ± 0.619
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.105 ± 0.213
5.058 ± 0.492
2.733 ± 0.543
5.467 ± 0.306
5.027 ± 0.429
4.775 ± 0.502
6.755 ± 0.419
4.775 ± 0.346
0.943 ± 0.104
4.493 ± 0.258
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here