Kribbella flavida (strain DSM 17836 / JCM 10339 / NBRC 14399)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Kribbellaceae; Kribbella; Kribbella flavida

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6940 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2PYL9|D2PYL9_KRIFD Transcriptional regulator SARP family OS=Kribbella flavida (strain DSM 17836 / JCM 10339 / NBRC 14399) OX=479435 GN=Kfla_0746 PE=3 SV=1
MM1 pKa = 7.66GYY3 pKa = 9.38WGYY6 pKa = 11.15LVAAKK11 pKa = 10.06SDD13 pKa = 3.87QPLHH17 pKa = 6.04EE18 pKa = 4.97LPEE21 pKa = 4.12LSAFGDD27 pKa = 3.88EE28 pKa = 4.38YY29 pKa = 11.91VEE31 pKa = 4.77VKK33 pKa = 10.2PLSNGWQQVWVAGANDD49 pKa = 3.66NPLTGAQSLATVTGHH64 pKa = 6.92PVLAALILDD73 pKa = 4.25SDD75 pKa = 4.62CGPVSAATPPGATWSGTLAKK95 pKa = 10.23SIAVDD100 pKa = 4.14SYY102 pKa = 12.11AMPDD106 pKa = 3.6DD107 pKa = 5.36NIPASAAASTFTEE120 pKa = 4.48WATSAALLADD130 pKa = 3.27QTLITRR136 pKa = 11.84ALAPTATDD144 pKa = 3.72PEE146 pKa = 4.53HH147 pKa = 7.06LFTLLLQATGIAAVNN162 pKa = 3.54

Molecular weight: 16.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2PKE3|D2PKE3_KRIFD Uncharacterized protein OS=Kribbella flavida (strain DSM 17836 / JCM 10339 / NBRC 14399) OX=479435 GN=Kfla_1353 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 5.34KK15 pKa = 10.47KK16 pKa = 8.84HH17 pKa = 5.5GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.28GRR40 pKa = 11.84QRR42 pKa = 11.84LAAA45 pKa = 4.56

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6940

0

6940

2258648

31

4780

325.5

34.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.203 ± 0.038

0.685 ± 0.007

6.052 ± 0.025

5.343 ± 0.029

2.829 ± 0.017

9.082 ± 0.032

2.064 ± 0.014

3.45 ± 0.02

2.161 ± 0.024

10.565 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.624 ± 0.012

1.987 ± 0.018

5.914 ± 0.031

3.206 ± 0.017

7.555 ± 0.03

5.256 ± 0.02

6.34 ± 0.025

8.905 ± 0.031

1.613 ± 0.013

2.164 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski