Lachnospiraceae bacterium JC7

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3167 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W3AS26|W3AS26_9FIRM Diguanylate cyclase (GGDEF) domain-containing protein OS=Lachnospiraceae bacterium JC7 OX=1165092 GN=UYO_0663 PE=4 SV=1
MM1 pKa = 7.87KK2 pKa = 10.61YY3 pKa = 10.42FLDD6 pKa = 4.26LSDD9 pKa = 3.35VTDD12 pKa = 3.92RR13 pKa = 11.84DD14 pKa = 3.53EE15 pKa = 5.71LYY17 pKa = 10.97DD18 pKa = 4.87AIEE21 pKa = 4.66DD22 pKa = 3.71QLPVPNYY29 pKa = 10.08FGRR32 pKa = 11.84NLDD35 pKa = 3.62ALYY38 pKa = 10.85DD39 pKa = 4.11DD40 pKa = 4.87LSEE43 pKa = 5.28VIDD46 pKa = 4.35DD47 pKa = 3.82CTLIVKK53 pKa = 9.99NFEE56 pKa = 3.91EE57 pKa = 4.72FAEE60 pKa = 4.56HH61 pKa = 7.4DD62 pKa = 3.51PQYY65 pKa = 10.92FKK67 pKa = 11.18KK68 pKa = 10.65FKK70 pKa = 9.36HH71 pKa = 4.83TLKK74 pKa = 10.8DD75 pKa = 3.64LMVEE79 pKa = 3.89IPDD82 pKa = 3.56FSVRR86 pKa = 11.84FSSDD90 pKa = 3.29DD91 pKa = 3.56EE92 pKa = 4.24EE93 pKa = 6.29SEE95 pKa = 5.47DD96 pKa = 5.33EE97 pKa = 3.92EE98 pKa = 6.69DD99 pKa = 3.69YY100 pKa = 11.81DD101 pKa = 4.15NGEE104 pKa = 4.02EE105 pKa = 4.62SEE107 pKa = 4.14YY108 pKa = 10.92DD109 pKa = 3.62YY110 pKa = 11.91SGDD113 pKa = 4.73DD114 pKa = 4.39YY115 pKa = 11.83SDD117 pKa = 4.42PDD119 pKa = 4.27DD120 pKa = 5.03NYY122 pKa = 11.28SDD124 pKa = 3.56SDD126 pKa = 4.47GYY128 pKa = 11.73DD129 pKa = 3.03KK130 pKa = 11.04MDD132 pKa = 3.37VNEE135 pKa = 4.05

Molecular weight: 15.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W3ALB5|W3ALB5_9FIRM Hydrolase TatD family OS=Lachnospiraceae bacterium JC7 OX=1165092 GN=UYO_2477 PE=4 SV=1
MM1 pKa = 7.64HH2 pKa = 7.44KK3 pKa = 10.88GKK5 pKa = 8.16MTFQPKK11 pKa = 7.64TRR13 pKa = 11.84QRR15 pKa = 11.84AKK17 pKa = 9.26VHH19 pKa = 5.55GFRR22 pKa = 11.84ARR24 pKa = 11.84MATAGGRR31 pKa = 11.84KK32 pKa = 8.88VLAARR37 pKa = 11.84RR38 pKa = 11.84AKK40 pKa = 10.1GRR42 pKa = 11.84AALTVV47 pKa = 3.28

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3167

0

3167

1040804

20

4402

328.6

36.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.496 ± 0.056

1.304 ± 0.015

6.288 ± 0.04

7.339 ± 0.047

4.221 ± 0.033

7.267 ± 0.047

1.749 ± 0.02

7.535 ± 0.042

6.637 ± 0.037

8.506 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.172 ± 0.026

4.494 ± 0.033

3.286 ± 0.022

2.695 ± 0.023

4.261 ± 0.032

6.46 ± 0.042

5.542 ± 0.041

6.819 ± 0.037

0.865 ± 0.015

4.063 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski