Amino acid dipepetide frequency for Lachnospiraceae bacterium JC7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.613AlaAla: 7.613 ± 0.151	0.986AlaCys: 0.986 ± 0.034	4.558AlaAsp: 4.558 ± 0.068	5.791AlaGlu: 5.791 ± 0.102	3.122AlaPhe: 3.122 ± 0.064	6.125AlaGly: 6.125 ± 0.09	1.143AlaHis: 1.143 ± 0.031	5.168AlaIle: 5.168 ± 0.078	4.501AlaLys: 4.501 ± 0.076	6.77AlaLeu: 6.77 ± 0.106	2.508AlaMet: 2.508 ± 0.049	2.66AlaAsn: 2.66 ± 0.056	2.093AlaPro: 2.093 ± 0.055	1.951AlaGln: 1.951 ± 0.046	2.685AlaArg: 2.685 ± 0.05	4.515AlaSer: 4.515 ± 0.083	3.554AlaThr: 3.554 ± 0.066	5.973AlaVal: 5.973 ± 0.087	0.595AlaTrp: 0.595 ± 0.022	2.653AlaTyr: 2.653 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.877CysAla: 0.877 ± 0.033	0.225CysCys: 0.225 ± 0.018	0.851CysAsp: 0.851 ± 0.032	0.81CysGlu: 0.81 ± 0.026	0.645CysPhe: 0.645 ± 0.028	1.34CysGly: 1.34 ± 0.043	0.268CysHis: 0.268 ± 0.015	1.056CysIle: 1.056 ± 0.035	0.652CysLys: 0.652 ± 0.026	1.012CysLeu: 1.012 ± 0.028	0.385CysMet: 0.385 ± 0.02	0.506CysAsn: 0.506 ± 0.023	0.557CysPro: 0.557 ± 0.027	0.306CysGln: 0.306 ± 0.019	0.582CysArg: 0.582 ± 0.025	0.846CysSer: 0.846 ± 0.029	0.689CysThr: 0.689 ± 0.025	0.856CysVal: 0.856 ± 0.03	0.089CysTrp: 0.089 ± 0.009	0.49CysTyr: 0.49 ± 0.024	0.0CysXaa: 0.0 ± 0.0
Asp	4.611AspAla: 4.611 ± 0.085	0.718AspCys: 0.718 ± 0.032	3.692AspAsp: 3.692 ± 0.084	5.164AspGlu: 5.164 ± 0.078	2.867AspPhe: 2.867 ± 0.057	4.933AspGly: 4.933 ± 0.091	1.124AspHis: 1.124 ± 0.035	5.209AspIle: 5.209 ± 0.076	3.934AspLys: 3.934 ± 0.08	4.802AspLeu: 4.802 ± 0.066	2.026AspMet: 2.026 ± 0.047	2.488AspAsn: 2.488 ± 0.049	2.335AspPro: 2.335 ± 0.054	1.382AspGln: 1.382 ± 0.036	2.802AspArg: 2.802 ± 0.064	3.836AspSer: 3.836 ± 0.071	3.251AspThr: 3.251 ± 0.061	4.106AspVal: 4.106 ± 0.073	0.606AspTrp: 0.606 ± 0.025	2.992AspTyr: 2.992 ± 0.058	0.001AspXaa: 0.001 ± 0.0
Glu	5.637GluAla: 5.637 ± 0.09	0.729GluCys: 0.729 ± 0.033	4.623GluAsp: 4.623 ± 0.061	6.206GluGlu: 6.206 ± 0.1	2.506GluPhe: 2.506 ± 0.056	4.294GluGly: 4.294 ± 0.065	1.32GluHis: 1.32 ± 0.042	5.461GluIle: 5.461 ± 0.091	6.413GluLys: 6.413 ± 0.094	6.654GluLeu: 6.654 ± 0.092	2.202GluMet: 2.202 ± 0.047	4.019GluAsn: 4.019 ± 0.071	1.802GluPro: 1.802 ± 0.049	2.218GluGln: 2.218 ± 0.041	3.307GluArg: 3.307 ± 0.068	3.953GluSer: 3.953 ± 0.071	4.316GluThr: 4.316 ± 0.083	4.201GluVal: 4.201 ± 0.064	0.614GluTrp: 0.614 ± 0.028	2.918GluTyr: 2.918 ± 0.055	0.0GluXaa: 0.0 ± 0.0
Phe	2.904PheAla: 2.904 ± 0.064	0.681PheCys: 0.681 ± 0.025	2.835PheAsp: 2.835 ± 0.046	2.561PheGlu: 2.561 ± 0.048	1.952PhePhe: 1.952 ± 0.051	3.385PheGly: 3.385 ± 0.063	0.789PheHis: 0.789 ± 0.029	3.286PheIle: 3.286 ± 0.063	2.219PheLys: 2.219 ± 0.048	3.662PheLeu: 3.662 ± 0.075	1.378PheMet: 1.378 ± 0.036	1.732PheAsn: 1.732 ± 0.043	1.384PhePro: 1.384 ± 0.041	1.057PheGln: 1.057 ± 0.034	1.898PheArg: 1.898 ± 0.046	3.092PheSer: 3.092 ± 0.051	2.425PheThr: 2.425 ± 0.052	2.79PheVal: 2.79 ± 0.064	0.39PheTrp: 0.39 ± 0.022	1.79PheTyr: 1.79 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	5.311GlyAla: 5.311 ± 0.096	1.139GlyCys: 1.139 ± 0.038	3.977GlyAsp: 3.977 ± 0.075	4.489GlyGlu: 4.489 ± 0.064	3.492GlyPhe: 3.492 ± 0.062	5.109GlyGly: 5.109 ± 0.101	1.318GlyHis: 1.318 ± 0.036	6.304GlyIle: 6.304 ± 0.089	5.138GlyLys: 5.138 ± 0.075	5.779GlyLeu: 5.779 ± 0.086	2.575GlyMet: 2.575 ± 0.054	3.373GlyAsn: 3.373 ± 0.056	1.501GlyPro: 1.501 ± 0.045	1.943GlyGln: 1.943 ± 0.049	2.981GlyArg: 2.981 ± 0.061	4.826GlySer: 4.826 ± 0.077	4.474GlyThr: 4.474 ± 0.083	5.002GlyVal: 5.002 ± 0.081	0.724GlyTrp: 0.724 ± 0.03	3.214GlyTyr: 3.214 ± 0.062	0.0GlyXaa: 0.0 ± 0.0
His	1.094HisAla: 1.094 ± 0.034	0.266HisCys: 0.266 ± 0.019	1.11HisAsp: 1.11 ± 0.037	1.226HisGlu: 1.226 ± 0.043	0.869HisPhe: 0.869 ± 0.029	1.266HisGly: 1.266 ± 0.034	0.445HisHis: 0.445 ± 0.045	1.43HisIle: 1.43 ± 0.04	0.987HisLys: 0.987 ± 0.031	1.313HisLeu: 1.313 ± 0.041	0.519HisMet: 0.519 ± 0.02	0.714HisAsn: 0.714 ± 0.027	0.895HisPro: 0.895 ± 0.035	0.478HisGln: 0.478 ± 0.02	0.754HisArg: 0.754 ± 0.028	1.049HisSer: 1.049 ± 0.032	0.981HisThr: 0.981 ± 0.031	1.156HisVal: 1.156 ± 0.034	0.159HisTrp: 0.159 ± 0.012	0.778HisTyr: 0.778 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.691IleAla: 5.691 ± 0.085	1.175IleCys: 1.175 ± 0.031	4.632IleAsp: 4.632 ± 0.067	4.89IleGlu: 4.89 ± 0.075	2.985IlePhe: 2.985 ± 0.061	5.254IleGly: 5.254 ± 0.085	1.373IleHis: 1.373 ± 0.041	5.536IleIle: 5.536 ± 0.08	4.659IleLys: 4.659 ± 0.066	6.685IleLeu: 6.685 ± 0.096	2.238IleMet: 2.238 ± 0.05	3.417IleAsn: 3.417 ± 0.063	3.324IlePro: 3.324 ± 0.062	1.925IleGln: 1.925 ± 0.042	3.574IleArg: 3.574 ± 0.069	5.398IleSer: 5.398 ± 0.078	4.291IleThr: 4.291 ± 0.064	4.92IleVal: 4.92 ± 0.09	0.556IleTrp: 0.556 ± 0.025	2.828IleTyr: 2.828 ± 0.054	0.0IleXaa: 0.0 ± 0.0
Lys	5.121LysAla: 5.121 ± 0.085	0.69LysCys: 0.69 ± 0.03	4.528LysAsp: 4.528 ± 0.076	5.738LysGlu: 5.738 ± 0.081	2.043LysPhe: 2.043 ± 0.043	4.122LysGly: 4.122 ± 0.074	1.132LysHis: 1.132 ± 0.037	4.539LysIle: 4.539 ± 0.076	5.671LysLys: 5.671 ± 0.096	5.622LysLeu: 5.622 ± 0.078	1.937LysMet: 1.937 ± 0.045	3.576LysAsn: 3.576 ± 0.059	1.962LysPro: 1.962 ± 0.039	1.824LysGln: 1.824 ± 0.044	2.84LysArg: 2.84 ± 0.057	3.628LysSer: 3.628 ± 0.059	3.865LysThr: 3.865 ± 0.076	4.096LysVal: 4.096 ± 0.066	0.593LysTrp: 0.593 ± 0.025	2.846LysTyr: 2.846 ± 0.055	0.0LysXaa: 0.0 ± 0.0
Leu	5.949LeuAla: 5.949 ± 0.081	1.263LeuCys: 1.263 ± 0.039	5.135LeuAsp: 5.135 ± 0.074	5.251LeuGlu: 5.251 ± 0.073	3.712LeuPhe: 3.712 ± 0.081	5.763LeuGly: 5.763 ± 0.086	1.425LeuHis: 1.425 ± 0.041	6.25LeuIle: 6.25 ± 0.08	5.929LeuLys: 5.929 ± 0.088	7.365LeuLeu: 7.365 ± 0.121	2.819LeuMet: 2.819 ± 0.055	3.908LeuAsn: 3.908 ± 0.06	3.337LeuPro: 3.337 ± 0.064	2.411LeuGln: 2.411 ± 0.046	3.873LeuArg: 3.873 ± 0.07	6.673LeuSer: 6.673 ± 0.096	4.913LeuThr: 4.913 ± 0.071	5.258LeuVal: 5.258 ± 0.083	0.682LeuTrp: 0.682 ± 0.03	3.143LeuTyr: 3.143 ± 0.06	0.0LeuXaa: 0.0 ± 0.0
Met	2.535MetAla: 2.535 ± 0.048	0.339MetCys: 0.339 ± 0.014	2.018MetAsp: 2.018 ± 0.042	2.153MetGlu: 2.153 ± 0.048	1.194MetPhe: 1.194 ± 0.037	2.153MetGly: 2.153 ± 0.05	0.491MetHis: 0.491 ± 0.022	2.404MetIle: 2.404 ± 0.054	2.432MetLys: 2.432 ± 0.05	2.75MetLeu: 2.75 ± 0.059	1.03MetMet: 1.03 ± 0.033	1.697MetAsn: 1.697 ± 0.044	1.117MetPro: 1.117 ± 0.032	0.96MetGln: 0.96 ± 0.036	1.292MetArg: 1.292 ± 0.033	1.987MetSer: 1.987 ± 0.048	1.839MetThr: 1.839 ± 0.038	2.126MetVal: 2.126 ± 0.044	0.212MetTrp: 0.212 ± 0.014	0.993MetTyr: 0.993 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	3.365AsnAla: 3.365 ± 0.069	0.634AsnCys: 0.634 ± 0.027	2.557AsnAsp: 2.557 ± 0.058	3.047AsnGlu: 3.047 ± 0.053	1.704AsnPhe: 1.704 ± 0.041	3.686AsnGly: 3.686 ± 0.071	0.84AsnHis: 0.84 ± 0.031	3.726AsnIle: 3.726 ± 0.069	2.498AsnLys: 2.498 ± 0.053	3.618AsnLeu: 3.618 ± 0.063	1.446AsnMet: 1.446 ± 0.039	1.945AsnAsn: 1.945 ± 0.065	1.936AsnPro: 1.936 ± 0.046	1.289AsnGln: 1.289 ± 0.037	2.076AsnArg: 2.076 ± 0.05	2.745AsnSer: 2.745 ± 0.059	2.396AsnThr: 2.396 ± 0.058	3.111AsnVal: 3.111 ± 0.06	0.408AsnTrp: 0.408 ± 0.021	1.914AsnTyr: 1.914 ± 0.048	0.001AsnXaa: 0.001 ± 0.001
Pro	2.327ProAla: 2.327 ± 0.055	0.359ProCys: 0.359 ± 0.019	2.635ProAsp: 2.635 ± 0.048	3.753ProGlu: 3.753 ± 0.064	1.488ProPhe: 1.488 ± 0.039	2.435ProGly: 2.435 ± 0.05	0.577ProHis: 0.577 ± 0.026	1.951ProIle: 1.951 ± 0.051	1.967ProLys: 1.967 ± 0.045	2.58ProLeu: 2.58 ± 0.057	0.98ProMet: 0.98 ± 0.028	1.201ProAsn: 1.201 ± 0.034	0.568ProPro: 0.568 ± 0.026	0.826ProGln: 0.826 ± 0.026	0.946ProArg: 0.946 ± 0.034	1.931ProSer: 1.931 ± 0.049	1.541ProThr: 1.541 ± 0.039	3.013ProVal: 3.013 ± 0.053	0.318ProTrp: 0.318 ± 0.018	1.464ProTyr: 1.464 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	2.063GlnAla: 2.063 ± 0.048	0.314GlnCys: 0.314 ± 0.018	1.612GlnAsp: 1.612 ± 0.043	2.057GlnGlu: 2.057 ± 0.049	1.084GlnPhe: 1.084 ± 0.03	1.802GlnGly: 1.802 ± 0.041	0.457GlnHis: 0.457 ± 0.022	1.955GlnIle: 1.955 ± 0.041	2.064GlnLys: 2.064 ± 0.053	2.224GlnLeu: 2.224 ± 0.045	0.954GlnMet: 0.954 ± 0.03	1.466GlnAsn: 1.466 ± 0.036	0.754GlnPro: 0.754 ± 0.031	0.931GlnGln: 0.931 ± 0.036	1.209GlnArg: 1.209 ± 0.037	1.526GlnSer: 1.526 ± 0.041	1.369GlnThr: 1.369 ± 0.04	1.681GlnVal: 1.681 ± 0.036	0.242GlnTrp: 0.242 ± 0.016	1.183GlnTyr: 1.183 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	2.664ArgAla: 2.664 ± 0.048	0.531ArgCys: 0.531 ± 0.024	2.677ArgAsp: 2.677 ± 0.064	3.394ArgGlu: 3.394 ± 0.072	1.977ArgPhe: 1.977 ± 0.043	2.369ArgGly: 2.369 ± 0.055	0.797ArgHis: 0.797 ± 0.029	3.563ArgIle: 3.563 ± 0.065	3.276ArgLys: 3.276 ± 0.055	3.629ArgLeu: 3.629 ± 0.063	1.485ArgMet: 1.485 ± 0.037	2.275ArgAsn: 2.275 ± 0.058	1.271ArgPro: 1.271 ± 0.043	1.267ArgGln: 1.267 ± 0.033	2.022ArgArg: 2.022 ± 0.052	2.439ArgSer: 2.439 ± 0.054	2.172ArgThr: 2.172 ± 0.049	2.565ArgVal: 2.565 ± 0.051	0.357ArgTrp: 0.357 ± 0.019	1.874ArgTyr: 1.874 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	4.635SerAla: 4.635 ± 0.08	0.742SerCys: 0.742 ± 0.03	4.409SerAsp: 4.409 ± 0.075	4.961SerGlu: 4.961 ± 0.088	3.084SerPhe: 3.084 ± 0.049	5.821SerGly: 5.821 ± 0.092	1.026SerHis: 1.026 ± 0.029	4.565SerIle: 4.565 ± 0.069	3.523SerLys: 3.523 ± 0.065	5.355SerLeu: 5.355 ± 0.069	2.069SerMet: 2.069 ± 0.043	2.476SerAsn: 2.476 ± 0.051	1.801SerPro: 1.801 ± 0.044	1.677SerGln: 1.677 ± 0.044	2.686SerArg: 2.686 ± 0.052	4.567SerSer: 4.567 ± 0.101	3.265SerThr: 3.265 ± 0.068	4.779SerVal: 4.779 ± 0.076	0.582SerTrp: 0.582 ± 0.024	2.575SerTyr: 2.575 ± 0.059	0.0SerXaa: 0.0 ± 0.0
Thr	4.762ThrAla: 4.762 ± 0.083	0.628ThrCys: 0.628 ± 0.025	3.499ThrAsp: 3.499 ± 0.069	4.336ThrGlu: 4.336 ± 0.075	2.237ThrPhe: 2.237 ± 0.057	4.854ThrGly: 4.854 ± 0.075	0.882ThrHis: 0.882 ± 0.031	3.934ThrIle: 3.934 ± 0.063	3.006ThrLys: 3.006 ± 0.059	4.803ThrLeu: 4.803 ± 0.076	1.524ThrMet: 1.524 ± 0.042	2.038ThrAsn: 2.038 ± 0.054	2.079ThrPro: 2.079 ± 0.044	1.268ThrGln: 1.268 ± 0.035	1.934ThrArg: 1.934 ± 0.041	3.273ThrSer: 3.273 ± 0.061	3.183ThrThr: 3.183 ± 0.07	4.723ThrVal: 4.723 ± 0.083	0.505ThrTrp: 0.505 ± 0.025	1.951ThrTyr: 1.951 ± 0.049	0.0ThrXaa: 0.0 ± 0.0
Val	4.634ValAla: 4.634 ± 0.089	0.936ValCys: 0.936 ± 0.031	4.04ValAsp: 4.04 ± 0.059	4.25ValGlu: 4.25 ± 0.073	3.031ValPhe: 3.031 ± 0.061	4.29ValGly: 4.29 ± 0.069	1.114ValHis: 1.114 ± 0.035	5.364ValIle: 5.364 ± 0.091	4.47ValLys: 4.47 ± 0.065	6.25ValLeu: 6.25 ± 0.09	2.199ValMet: 2.199 ± 0.053	3.097ValAsn: 3.097 ± 0.06	2.576ValPro: 2.576 ± 0.049	1.749ValGln: 1.749 ± 0.045	2.848ValArg: 2.848 ± 0.055	5.242ValSer: 5.242 ± 0.078	4.247ValThr: 4.247 ± 0.074	4.623ValVal: 4.623 ± 0.082	0.541ValTrp: 0.541 ± 0.027	2.688ValTyr: 2.688 ± 0.053	0.0ValXaa: 0.0 ± 0.0
Trp	0.539TrpAla: 0.539 ± 0.024	0.123TrpCys: 0.123 ± 0.011	0.573TrpAsp: 0.573 ± 0.032	0.497TrpGlu: 0.497 ± 0.024	0.423TrpPhe: 0.423 ± 0.024	0.556TrpGly: 0.556 ± 0.022	0.183TrpHis: 0.183 ± 0.012	0.671TrpIle: 0.671 ± 0.027	0.676TrpLys: 0.676 ± 0.032	0.753TrpLeu: 0.753 ± 0.029	0.323TrpMet: 0.323 ± 0.016	0.518TrpAsn: 0.518 ± 0.023	0.21TrpPro: 0.21 ± 0.015	0.304TrpGln: 0.304 ± 0.019	0.358TrpArg: 0.358 ± 0.02	0.463TrpSer: 0.463 ± 0.023	0.456TrpThr: 0.456 ± 0.024	0.496TrpVal: 0.496 ± 0.023	0.114TrpTrp: 0.114 ± 0.01	0.415TrpTyr: 0.415 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.633TyrAla: 2.633 ± 0.05	0.564TyrCys: 0.564 ± 0.023	2.919TyrAsp: 2.919 ± 0.062	2.821TyrGlu: 2.821 ± 0.054	1.795TyrPhe: 1.795 ± 0.044	3.112TyrGly: 3.112 ± 0.056	0.784TyrHis: 0.784 ± 0.024	2.98TyrIle: 2.98 ± 0.053	2.355TyrLys: 2.355 ± 0.047	3.432TyrLeu: 3.432 ± 0.06	1.125TyrMet: 1.125 ± 0.035	1.837TyrAsn: 1.837 ± 0.047	1.36TyrPro: 1.36 ± 0.038	1.183TyrGln: 1.183 ± 0.034	1.938TyrArg: 1.938 ± 0.049	2.607TyrSer: 2.607 ± 0.054	2.193TyrThr: 2.193 ± 0.057	2.71TyrVal: 2.71 ± 0.048	0.361TyrTrp: 0.361 ± 0.021	1.926TyrTyr: 1.926 ± 0.056	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3167 proteins (1040804 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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