Wenyingzhuangia fucanilytica
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2747 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B1Y4Y1|A0A1B1Y4Y1_9FLAO Cyclic nucleotide-binding protein OS=Wenyingzhuangia fucanilytica OX=1790137 GN=AXE80_05665 PE=4 SV=1
MM1 pKa = 7.26 KK2 pKa = 9.52 TLKK5 pKa = 10.74 LITALMFVSFLGFISCQKK23 pKa = 10.67 EE24 pKa = 3.42 IDD26 pKa = 4.01 EE27 pKa = 5.12 EE28 pKa = 4.25 NGQNPNTNSVNSEE41 pKa = 3.94 TAKK44 pKa = 10.62 NLQRR48 pKa = 11.84 TAMYY52 pKa = 10.27 DD53 pKa = 3.3 GSFDD57 pKa = 4.29 DD58 pKa = 5.88 FLDD61 pKa = 4.22 GTSCSSVLFPVTVWVNNQKK80 pKa = 10.49 IILTNKK86 pKa = 9.8 NDD88 pKa = 3.54 YY89 pKa = 10.86 QLVLNILGEE98 pKa = 4.35 VINDD102 pKa = 3.16 EE103 pKa = 4.19 DD104 pKa = 4.61 TITFQFPISVKK115 pKa = 9.92 LSNYY119 pKa = 9.4 TEE121 pKa = 4.15 VQVEE125 pKa = 4.03 NQAEE129 pKa = 4.16 YY130 pKa = 11.16 NAIKK134 pKa = 9.05 DD135 pKa = 3.62 TCEE138 pKa = 3.32 EE139 pKa = 4.09 AEE141 pKa = 4.2 AEE143 pKa = 4.15 KK144 pKa = 11.03 EE145 pKa = 4.07 NAINCLKK152 pKa = 9.94 MNYY155 pKa = 8.86 PISILTYY162 pKa = 10.33 NISLEE167 pKa = 4.27 QTSTTVIEE175 pKa = 4.78 SNQEE179 pKa = 3.63 LYY181 pKa = 10.63 TYY183 pKa = 9.73 MSNFNEE189 pKa = 4.36 DD190 pKa = 3.04 EE191 pKa = 4.3 KK192 pKa = 11.42 FSIKK196 pKa = 10.68 YY197 pKa = 8.91 PISATLSNDD206 pKa = 3.25 TEE208 pKa = 4.57 VNISSDD214 pKa = 3.9 AEE216 pKa = 4.34 FKK218 pKa = 11.05 SHH220 pKa = 7.49 ISDD223 pKa = 3.88 CLGDD227 pKa = 4.48 EE228 pKa = 4.36 DD229 pKa = 5.45 TMDD232 pKa = 4.91 DD233 pKa = 3.73 AQEE236 pKa = 4.18 DD237 pKa = 4.57 AEE239 pKa = 4.31 TLEE242 pKa = 4.66 NILVEE247 pKa = 4.17 GTFRR251 pKa = 11.84 VQSYY255 pKa = 10.98 INGGVDD261 pKa = 3.35 TANTYY266 pKa = 11.37 ANYY269 pKa = 9.83 TIDD272 pKa = 3.57 FANDD276 pKa = 3.66 LTCSATNTANALAEE290 pKa = 4.22 KK291 pKa = 10.9 AEE293 pKa = 4.42 GTFSVTSEE301 pKa = 4.02 MEE303 pKa = 3.92 VFLTLEE309 pKa = 4.42 FSSEE313 pKa = 4.18 ANFEE317 pKa = 4.46 LLNDD321 pKa = 3.35 TWTVTNYY328 pKa = 10.09 SSSSITLQSTTNTSTALVLNQII350 pKa = 4.41
Molecular weight: 38.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.846
IPC_protein 3.821
Toseland 3.63
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.668
Rodwell 3.643
Grimsley 3.541
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.05
Thurlkill 3.656
EMBOSS 3.681
Sillero 3.935
Patrickios 0.947
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.827
Protein with the highest isoelectric point:
>tr|A0A1B1Y7W9|A0A1B1Y7W9_9FLAO 30S ribosomal protein S12 OS=Wenyingzhuangia fucanilytica OX=1790137 GN=rpsL PE=3 SV=1
MM1 pKa = 7.54 SVRR4 pKa = 11.84 KK5 pKa = 9.68 LKK7 pKa = 10.5 PITPGQRR14 pKa = 11.84 FRR16 pKa = 11.84 VVNGFDD22 pKa = 5.49 AITTDD27 pKa = 3.56 KK28 pKa = 11.07 PEE30 pKa = 4.39 KK31 pKa = 10.58 SLLAPNKK38 pKa = 10.19 RR39 pKa = 11.84 SGGRR43 pKa = 11.84 NNSGRR48 pKa = 11.84 MTMRR52 pKa = 11.84 YY53 pKa = 9.71 VGGGHH58 pKa = 6.68 KK59 pKa = 9.72 KK60 pKa = 9.76 RR61 pKa = 11.84 YY62 pKa = 9.53 RR63 pKa = 11.84 IIDD66 pKa = 3.52 FNRR69 pKa = 11.84 DD70 pKa = 3.14 KK71 pKa = 11.15 FGVEE75 pKa = 3.76 ATVKK79 pKa = 9.91 TIEE82 pKa = 4.03 YY83 pKa = 9.86 DD84 pKa = 3.47 PNRR87 pKa = 11.84 TAFIALVEE95 pKa = 4.25 YY96 pKa = 10.5 TDD98 pKa = 3.61 GEE100 pKa = 4.17 KK101 pKa = 10.32 RR102 pKa = 11.84 YY103 pKa = 10.63 VIAQAGLQVGQTIVSNNDD121 pKa = 3.32 AITPDD126 pKa = 3.47 VGNAMPLANIPLGTTICCIEE146 pKa = 4.14 LRR148 pKa = 11.84 PGQGAVMARR157 pKa = 11.84 SAGSFAQLMAKK168 pKa = 8.96 EE169 pKa = 4.42 GKK171 pKa = 9.47 YY172 pKa = 9.15 ATVKK176 pKa = 10.52 LPSGEE181 pKa = 4.06 TRR183 pKa = 11.84 LILLTCIATIGVVSNSDD200 pKa = 3.21 HH201 pKa = 6.09 QLLVSGKK208 pKa = 9.49 AGRR211 pKa = 11.84 SRR213 pKa = 11.84 WLGRR217 pKa = 11.84 RR218 pKa = 11.84 PRR220 pKa = 11.84 TNAVRR225 pKa = 11.84 MNPVDD230 pKa = 3.4 HH231 pKa = 7.07 PMGGGEE237 pKa = 4.08 GRR239 pKa = 11.84 SSGGHH244 pKa = 4.7 PRR246 pKa = 11.84 SRR248 pKa = 11.84 NGIPAKK254 pKa = 10.28 GYY256 pKa = 7.21 KK257 pKa = 8.8 TRR259 pKa = 11.84 SKK261 pKa = 9.63 TKK263 pKa = 10.38 ASNKK267 pKa = 9.94 YY268 pKa = 8.99 ILEE271 pKa = 4.09 RR272 pKa = 11.84 RR273 pKa = 11.84 KK274 pKa = 10.2 KK275 pKa = 9.94
Molecular weight: 30.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.809
IPC_protein 10.511
Toseland 10.818
ProMoST 10.496
Dawson 10.906
Bjellqvist 10.584
Wikipedia 11.096
Rodwell 11.213
Grimsley 10.95
Solomon 11.008
Lehninger 10.979
Nozaki 10.804
DTASelect 10.584
Thurlkill 10.818
EMBOSS 11.228
Sillero 10.833
Patrickios 10.921
IPC_peptide 11.023
IPC2_peptide 9.458
IPC2.peptide.svr19 8.581
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2747
0
2747
1031829
54
2341
375.6
42.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.177 ± 0.043
0.756 ± 0.011
5.519 ± 0.032
6.486 ± 0.04
5.026 ± 0.03
6.461 ± 0.041
1.876 ± 0.019
7.883 ± 0.046
8.25 ± 0.05
9.008 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.103 ± 0.019
6.597 ± 0.05
3.397 ± 0.023
3.353 ± 0.021
3.066 ± 0.025
6.332 ± 0.038
5.983 ± 0.04
6.394 ± 0.037
1.115 ± 0.02
4.218 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here