Streptococcus phage phiARI0746

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A141DZE8|A0A141DZE8_9CAUD HD_4 domain protein OS=Streptococcus phage phiARI0746 OX=1701837 GN=phiARI0746_14 PE=4 SV=1
MM1 pKa = 7.28DD2 pKa = 4.2TARR5 pKa = 11.84IGITNVEE12 pKa = 4.08FSGTGEE18 pKa = 4.01NDD20 pKa = 3.15SATVKK25 pKa = 10.92LEE27 pKa = 3.91LNIYY31 pKa = 9.84GADD34 pKa = 3.23TFSAIEE40 pKa = 4.46LLPKK44 pKa = 10.13ILTDD48 pKa = 3.24IHH50 pKa = 6.43SLSYY54 pKa = 10.72EE55 pKa = 3.71VDD57 pKa = 3.08

Molecular weight: 6.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A141DZH1|A0A141DZH1_9CAUD AntA domain protein OS=Streptococcus phage phiARI0746 OX=1701837 GN=phiARI0746_39 PE=4 SV=1
MM1 pKa = 7.07EE2 pKa = 4.5TVQIVRR8 pKa = 11.84IKK10 pKa = 10.86DD11 pKa = 3.46VIIEE15 pKa = 4.43KK16 pKa = 10.29ISANDD21 pKa = 3.46EE22 pKa = 3.71EE23 pKa = 4.67LKK25 pKa = 10.87RR26 pKa = 11.84IFGCSKK32 pKa = 9.19RR33 pKa = 11.84QAGEE37 pKa = 3.54RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84EE41 pKa = 4.1MKK43 pKa = 10.42KK44 pKa = 10.49LPSQQKK50 pKa = 9.47HH51 pKa = 5.67LLDD54 pKa = 4.14SGQLVTIKK62 pKa = 10.74GFYY65 pKa = 10.14EE66 pKa = 3.7YY67 pKa = 10.66LQYY70 pKa = 10.73RR71 pKa = 11.84GSQPWKK77 pKa = 10.0KK78 pKa = 10.8EE79 pKa = 3.44MAKK82 pKa = 8.52TVKK85 pKa = 8.0MTRR88 pKa = 3.25

Molecular weight: 10.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

8356

57

1217

199.0

22.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.109 ± 0.765

0.598 ± 0.141

6.45 ± 0.341

8.054 ± 0.655

4.165 ± 0.32

6.462 ± 0.806

1.328 ± 0.167

6.989 ± 0.371

8.832 ± 0.483

8.114 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.465 ± 0.203

5.278 ± 0.296

2.657 ± 0.171

4.225 ± 0.352

3.997 ± 0.389

5.948 ± 0.306

5.972 ± 0.288

6.546 ± 0.291

1.053 ± 0.175

3.758 ± 0.469
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski