Capybara microvirus Cap3_SP_393
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVS9|A0A4V1FVS9_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_393 OX=2585443 PE=4 SV=1
MM1 pKa = 6.99 STEE4 pKa = 4.16 KK5 pKa = 10.33 EE6 pKa = 3.97 QKK8 pKa = 10.17 KK9 pKa = 10.06 KK10 pKa = 10.56 FFRR13 pKa = 11.84 SLIEE17 pKa = 3.83 RR18 pKa = 11.84 TKK20 pKa = 10.82 TKK22 pKa = 10.54 LPPKK26 pKa = 9.75 AVSVVLGFNNVVTLQTDD43 pKa = 4.11 CPNPEE48 pKa = 3.92 PDD50 pKa = 4.47 FRR52 pKa = 11.84 DD53 pKa = 3.23 ISLGAQAEE61 pKa = 4.55 SGSFLGEE68 pKa = 3.86 VNTNNGDD75 pKa = 2.85 IFTAHH80 pKa = 7.41 KK81 pKa = 10.27 LAEE84 pKa = 4.41 DD85 pKa = 3.66 VLNNSDD91 pKa = 3.23 NN92 pKa = 3.59
Molecular weight: 10.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.323
IPC2_protein 5.397
IPC_protein 5.194
Toseland 5.13
ProMoST 5.321
Dawson 5.181
Bjellqvist 5.308
Wikipedia 5.054
Rodwell 5.092
Grimsley 5.054
Solomon 5.169
Lehninger 5.13
Nozaki 5.296
DTASelect 5.448
Thurlkill 5.13
EMBOSS 5.092
Sillero 5.372
Patrickios 4.762
IPC_peptide 5.181
IPC2_peptide 5.359
IPC2.peptide.svr19 5.522
Protein with the highest isoelectric point:
>tr|A0A4P8W8G9|A0A4P8W8G9_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_393 OX=2585443 PE=3 SV=1
MM1 pKa = 7.77 LIKK4 pKa = 10.82 NNSKK8 pKa = 9.24 TSYY11 pKa = 9.85 CSKK14 pKa = 10.23 KK15 pKa = 10.5 QYY17 pKa = 11.14 ININDD22 pKa = 3.74 SYY24 pKa = 11.53 NYY26 pKa = 10.13 LSSFQAYY33 pKa = 7.99 VRR35 pKa = 11.84 TYY37 pKa = 10.33 YY38 pKa = 10.59 DD39 pKa = 3.29 YY40 pKa = 11.47 QYY42 pKa = 10.71 VQSNYY47 pKa = 9.26 GVAYY51 pKa = 9.63 FYY53 pKa = 10.87 TLTFNDD59 pKa = 4.28 LNLNTINYY67 pKa = 9.52 NGISINTHH75 pKa = 3.56 NHH77 pKa = 5.83 KK78 pKa = 10.42 LLHH81 pKa = 5.75 LWIDD85 pKa = 3.51 NVYY88 pKa = 10.63 KK89 pKa = 10.64 ILLRR93 pKa = 11.84 KK94 pKa = 9.41 FDD96 pKa = 3.86 CKK98 pKa = 10.99 SRR100 pKa = 11.84 FFITSEE106 pKa = 3.93 CGEE109 pKa = 4.37 GKK111 pKa = 10.57 GSRR114 pKa = 11.84 KK115 pKa = 9.74 KK116 pKa = 11.01 GNNPHH121 pKa = 5.67 YY122 pKa = 10.51 HH123 pKa = 6.04 VILYY127 pKa = 7.22 VIPTIEE133 pKa = 4.22 NMLPTSKK140 pKa = 10.72 EE141 pKa = 3.56 MLSIVRR147 pKa = 11.84 YY148 pKa = 8.58 CWQGTDD154 pKa = 4.47 LDD156 pKa = 4.31 LTLKK160 pKa = 9.84 QNSYY164 pKa = 9.84 IAKK167 pKa = 7.37 TCKK170 pKa = 10.29 NGILKK175 pKa = 10.14 EE176 pKa = 4.09 GKK178 pKa = 9.38 NLGLIEE184 pKa = 4.31 SLNALSYY191 pKa = 8.23 VCKK194 pKa = 10.45 YY195 pKa = 9.33 VSKK198 pKa = 10.68 DD199 pKa = 3.03 IEE201 pKa = 4.57 TKK203 pKa = 8.8 EE204 pKa = 3.95 HH205 pKa = 6.25 YY206 pKa = 10.44 DD207 pKa = 3.35 IIKK210 pKa = 10.21 NYY212 pKa = 10.5 YY213 pKa = 8.0 YY214 pKa = 11.13 YY215 pKa = 11.21 KK216 pKa = 10.58 FYY218 pKa = 11.18 NEE220 pKa = 4.7 KK221 pKa = 10.57 LLSCLKK227 pKa = 10.8 CIDD230 pKa = 3.56 NLIYY234 pKa = 10.36 ILQKK238 pKa = 10.54 SGLNEE243 pKa = 4.24 TYY245 pKa = 10.66 CNEE248 pKa = 3.88 IQLDD252 pKa = 4.2 NINSIDD258 pKa = 3.85 EE259 pKa = 4.34 FEE261 pKa = 5.44 LYY263 pKa = 10.99 LFDD266 pKa = 4.9 FVKK269 pKa = 10.78 NNHH272 pKa = 6.12 GSTEE276 pKa = 4.48 FILCNINKK284 pKa = 9.89 LYY286 pKa = 10.79 KK287 pKa = 10.28 DD288 pKa = 3.16 IYY290 pKa = 7.77 NTYY293 pKa = 10.13 RR294 pKa = 11.84 LIHH297 pKa = 6.45 KK298 pKa = 9.79 EE299 pKa = 3.88 YY300 pKa = 11.01 DD301 pKa = 3.26 FLDD304 pKa = 4.39 YY305 pKa = 10.52 IPKK308 pKa = 8.96 TFYY311 pKa = 10.17 PQMMGFKK318 pKa = 9.85 LAKK321 pKa = 9.21 QNIVEE326 pKa = 4.48 TCSGTFIKK334 pKa = 9.83 IDD336 pKa = 3.46 NKK338 pKa = 10.43 LCPISKK344 pKa = 10.26 CFFNKK349 pKa = 10.32 LYY351 pKa = 8.8 KK352 pKa = 9.28 THH354 pKa = 6.79 LRR356 pKa = 11.84 SCPSYY361 pKa = 11.09 SRR363 pKa = 11.84 YY364 pKa = 10.58 NYY366 pKa = 10.68 DD367 pKa = 3.93 GINYY371 pKa = 9.36 LCRR374 pKa = 11.84 TIQNRR379 pKa = 11.84 IDD381 pKa = 3.25 ILAAKK386 pKa = 9.97 ANLYY390 pKa = 11.09 CNDD393 pKa = 3.33 KK394 pKa = 10.7 NICKK398 pKa = 9.99 LYY400 pKa = 10.94 SMYY403 pKa = 11.12 KK404 pKa = 9.88 LVYY407 pKa = 7.55 EE408 pKa = 4.53 HH409 pKa = 7.29 RR410 pKa = 11.84 LIPISDD416 pKa = 3.7 FNKK419 pKa = 9.44 PLEE422 pKa = 3.95 MLKK425 pKa = 10.38 DD426 pKa = 3.58 YY427 pKa = 10.95 KK428 pKa = 11.21 NYY430 pKa = 9.89 MEE432 pKa = 5.15 LEE434 pKa = 4.47 SIFSFRR440 pKa = 11.84 RR441 pKa = 11.84 TFGIEE446 pKa = 4.01 LNLDD450 pKa = 3.61 NYY452 pKa = 9.9 EE453 pKa = 4.08 KK454 pKa = 10.87 AIMHH458 pKa = 5.98 PQFYY462 pKa = 9.35 PYY464 pKa = 10.52 RR465 pKa = 11.84 SIFTSISNVIEE476 pKa = 4.06 KK477 pKa = 10.68 SEE479 pKa = 4.24 IADD482 pKa = 3.64 KK483 pKa = 9.27 QHH485 pKa = 6.76 KK486 pKa = 9.64 EE487 pKa = 3.71 EE488 pKa = 4.09 NFEE491 pKa = 4.27 HH492 pKa = 6.62 KK493 pKa = 10.58 KK494 pKa = 10.05 HH495 pKa = 5.69 VKK497 pKa = 10.03 KK498 pKa = 10.77 LLNSYY503 pKa = 9.8 RR504 pKa = 11.84 YY505 pKa = 9.44 EE506 pKa = 4.14 YY507 pKa = 11.42
Molecular weight: 60.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.62
IPC2_protein 8.097
IPC_protein 7.892
Toseland 7.702
ProMoST 8.478
Dawson 8.653
Bjellqvist 8.858
Wikipedia 8.668
Rodwell 8.697
Grimsley 7.732
Solomon 8.697
Lehninger 8.682
Nozaki 8.916
DTASelect 8.639
Thurlkill 8.682
EMBOSS 8.814
Sillero 8.96
Patrickios 1.825
IPC_peptide 8.682
IPC2_peptide 7.702
IPC2.peptide.svr19 7.96
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1563
92
583
390.8
44.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.566 ± 1.707
1.536 ± 0.718
4.798 ± 0.834
5.31 ± 1.061
5.054 ± 0.692
4.607 ± 0.549
1.536 ± 0.481
7.102 ± 0.959
7.422 ± 1.179
8.893 ± 0.77
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.791 ± 0.209
9.341 ± 0.517
3.263 ± 0.783
4.031 ± 1.181
3.263 ± 0.231
9.277 ± 0.971
6.206 ± 0.795
4.287 ± 0.836
0.512 ± 0.115
6.206 ± 1.884
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here