Diaminobutyricibacter tongyongensis
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3799 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6L9XSM7|A0A6L9XSM7_9MICO Response regulator transcription factor OS=Diaminobutyricibacter tongyongensis OX=1268043 GN=G3T36_00865 PE=4 SV=1
MM1 pKa = 7.84 DD2 pKa = 3.86 TTDD5 pKa = 3.54 GATGGTGIDD14 pKa = 3.6 QGNVQQPSAAHH25 pKa = 5.86 SGVSRR30 pKa = 11.84 GRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 VVVLSAALAATVVAGATAGVAANGFASRR63 pKa = 11.84 SAAAASTSSTSANIHH78 pKa = 5.86 NALGPGSYY86 pKa = 10.58 GYY88 pKa = 10.64 GGYY91 pKa = 10.92 GSGGYY96 pKa = 9.59 GGYY99 pKa = 10.35 GGYY102 pKa = 10.94 ASGGYY107 pKa = 10.24 GSGGYY112 pKa = 9.86 GSSSGSTGSAALASATPAEE131 pKa = 4.41 EE132 pKa = 4.11 VGVVDD137 pKa = 3.51 ITSEE141 pKa = 3.9 LTYY144 pKa = 10.61 QQAEE148 pKa = 4.28 SAGTGLVLTSDD159 pKa = 4.83 GEE161 pKa = 4.28 ILTNNHH167 pKa = 4.8 VVAGATSISVTVVSTGATFTATVVGTDD194 pKa = 3.25 ATDD197 pKa = 4.88 DD198 pKa = 3.57 IAVLHH203 pKa = 6.53 LSNASGLSTAHH214 pKa = 7.36 LDD216 pKa = 3.85 TSNSPAVGDD225 pKa = 3.7 AVTGVGNAGGTGGTPSASPGTVTALDD251 pKa = 3.59 QTITTQAEE259 pKa = 4.24 GSAASEE265 pKa = 4.16 TLNGLIEE272 pKa = 4.27 TDD274 pKa = 3.59 ADD276 pKa = 3.55 IQAGDD281 pKa = 3.23 SGGPLFNSADD291 pKa = 3.58 QVIGIDD297 pKa = 3.32 TAAASGGATPAGYY310 pKa = 10.33 AIPIRR315 pKa = 11.84 SALAVAGEE323 pKa = 4.32 IVAGHH328 pKa = 7.03 ASSTVTIGYY337 pKa = 7.9 PAFLGVEE344 pKa = 4.42 VGSSADD350 pKa = 3.31 GTAAATPGAAIAGVIDD366 pKa = 4.3 GSPAATAGLAAGDD379 pKa = 4.44 TITALNGTTIDD390 pKa = 3.63 SQQALTATLQTLAPGQRR407 pKa = 11.84 VSIAWTDD414 pKa = 3.29 QSGASQSAPITLASGPVAA432 pKa = 4.2
Molecular weight: 40.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.772
IPC2_protein 3.948
IPC_protein 3.948
Toseland 3.732
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.884
Rodwell 3.77
Grimsley 3.643
Solomon 3.935
Lehninger 3.897
Nozaki 4.062
DTASelect 4.317
Thurlkill 3.783
EMBOSS 3.897
Sillero 4.075
Patrickios 0.871
IPC_peptide 3.935
IPC2_peptide 4.05
IPC2.peptide.svr19 3.947
Protein with the highest isoelectric point:
>tr|A0A6L9XVF8|A0A6L9XVF8_9MICO Alpha/beta hydrolase OS=Diaminobutyricibacter tongyongensis OX=1268043 GN=G3T36_05890 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 KK12 pKa = 8.98 RR13 pKa = 11.84 AKK15 pKa = 8.46 THH17 pKa = 5.11 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILAARR35 pKa = 11.84 RR36 pKa = 11.84 TKK38 pKa = 10.79 GRR40 pKa = 11.84 TKK42 pKa = 10.84 LSAA45 pKa = 3.61
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3799
0
3799
1213229
27
10786
319.4
34.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.001 ± 0.075
0.493 ± 0.009
6.007 ± 0.038
5.36 ± 0.053
3.321 ± 0.027
8.801 ± 0.056
1.976 ± 0.018
4.87 ± 0.031
2.199 ± 0.029
10.032 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.736 ± 0.02
2.328 ± 0.031
5.337 ± 0.033
2.846 ± 0.025
6.919 ± 0.058
6.06 ± 0.046
6.268 ± 0.074
8.813 ± 0.039
1.511 ± 0.023
2.122 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here