Inachis io cypovirus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Cypovirus; Cypovirus 2

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|W6EJ67|W6EJ67_9REOV VP5 OS=Inachis io cypovirus 2 OX=1382295 PE=4 SV=1
MM1 pKa = 8.0AYY3 pKa = 8.99PAQGYY8 pKa = 9.09VLDD11 pKa = 3.76EE12 pKa = 4.34RR13 pKa = 11.84FNFEE17 pKa = 3.91YY18 pKa = 10.34AIKK21 pKa = 10.42LLRR24 pKa = 11.84EE25 pKa = 3.54ADD27 pKa = 3.44INGEE31 pKa = 4.26TKK33 pKa = 10.76DD34 pKa = 3.62KK35 pKa = 11.41VSVAEE40 pKa = 4.37LRR42 pKa = 11.84DD43 pKa = 4.31FIIKK47 pKa = 9.43EE48 pKa = 4.0AQDD51 pKa = 3.12EE52 pKa = 4.32QFAVRR57 pKa = 11.84EE58 pKa = 4.17RR59 pKa = 11.84PLPPAQVRR67 pKa = 11.84GISARR72 pKa = 11.84VTVCPINSILCQLFCGDD89 pKa = 3.61GFYY92 pKa = 10.94AQSFIKK98 pKa = 10.53LMAIGQMYY106 pKa = 10.32ANQITAEE113 pKa = 4.05AFTVEE118 pKa = 3.99EE119 pKa = 4.24GQFYY123 pKa = 10.81VNEE126 pKa = 4.41GGIGPDD132 pKa = 3.02STKK135 pKa = 10.72YY136 pKa = 8.47PTAHH140 pKa = 6.36VAPLMEE146 pKa = 4.76AFRR149 pKa = 11.84RR150 pKa = 11.84CANAMDD156 pKa = 4.26STIDD160 pKa = 3.3PHH162 pKa = 6.69GTIQQLYY169 pKa = 9.69ALMEE173 pKa = 4.41EE174 pKa = 4.5TTHH177 pKa = 5.8MEE179 pKa = 4.08TWFMDD184 pKa = 3.7PSVITPRR191 pKa = 11.84QITSSIQNWNLFAAFVDD208 pKa = 4.03MSEE211 pKa = 4.36PNPVFPVATWQKK223 pKa = 10.97LLDD226 pKa = 3.53ILRR229 pKa = 11.84AVVLTFITSVSSTAVFDD246 pKa = 4.19EE247 pKa = 5.09GPPLQISFLLPSYY260 pKa = 10.75QLTNPRR266 pKa = 11.84LVNFTRR272 pKa = 11.84SYY274 pKa = 10.9QNLRR278 pKa = 11.84QQYY281 pKa = 9.2ISWILRR287 pKa = 11.84TVFIPFLEE295 pKa = 3.97RR296 pKa = 11.84LRR298 pKa = 11.84RR299 pKa = 11.84FIVGNNARR307 pKa = 11.84IARR310 pKa = 11.84TTFAGEE316 pKa = 3.93KK317 pKa = 9.74KK318 pKa = 10.31VAIPYY323 pKa = 10.25FGDD326 pKa = 3.45DD327 pKa = 4.96RR328 pKa = 11.84EE329 pKa = 4.38MKK331 pKa = 9.69TNNTIQVVDD340 pKa = 3.2KK341 pKa = 10.71RR342 pKa = 11.84GRR344 pKa = 11.84ANYY347 pKa = 9.51DD348 pKa = 2.96AYY350 pKa = 10.07KK351 pKa = 9.42YY352 pKa = 9.05TLEE355 pKa = 3.78NHH357 pKa = 6.61LLFGEE362 pKa = 4.39DD363 pKa = 2.93GRR365 pKa = 11.84RR366 pKa = 11.84PRR368 pKa = 11.84MFTKK372 pKa = 10.32TLVQNDD378 pKa = 4.17DD379 pKa = 3.41LSTFHH384 pKa = 6.46VQIFNSFTDD393 pKa = 4.3TIYY396 pKa = 9.67GTNKK400 pKa = 9.98NVTAAYY406 pKa = 8.25DD407 pKa = 3.53TMSALVAKK415 pKa = 7.24YY416 pKa = 8.85TPINYY421 pKa = 7.54NTLIVEE427 pKa = 4.37SLDD430 pKa = 3.44DD431 pKa = 3.83YY432 pKa = 12.01NRR434 pKa = 11.84INLKK438 pKa = 9.47TGDD441 pKa = 3.55ISQVAVWFLTEE452 pKa = 3.37YY453 pKa = 10.4SRR455 pKa = 11.84PYY457 pKa = 10.65VYY459 pKa = 10.52FGVASLYY466 pKa = 11.19LLDD469 pKa = 4.47DD470 pKa = 4.37TIIAYY475 pKa = 7.47TVHH478 pKa = 6.19TNVGRR483 pKa = 11.84YY484 pKa = 9.0HH485 pKa = 6.68EE486 pKa = 4.67GEE488 pKa = 3.8KK489 pKa = 10.06TFAFEE494 pKa = 4.13EE495 pKa = 4.7CTAMHH500 pKa = 7.05MFNILYY506 pKa = 10.03SLQVCLDD513 pKa = 3.55PEE515 pKa = 4.31AFLTNGEE522 pKa = 4.19MDD524 pKa = 3.17KK525 pKa = 11.25HH526 pKa = 6.68EE527 pKa = 4.29YY528 pKa = 8.7TLDD531 pKa = 3.39YY532 pKa = 10.6RR533 pKa = 11.84VWRR536 pKa = 11.84EE537 pKa = 3.69ALSQEE542 pKa = 5.47LKK544 pKa = 9.01TCWRR548 pKa = 11.84PLCDD552 pKa = 3.06HH553 pKa = 6.11YY554 pKa = 11.72ARR556 pKa = 11.84RR557 pKa = 11.84NVQDD561 pKa = 3.59TPTVAMAEE569 pKa = 4.35MKK571 pKa = 10.02EE572 pKa = 4.05LMGAYY577 pKa = 9.05PYY579 pKa = 9.37TAIYY583 pKa = 9.94KK584 pKa = 10.41FEE586 pKa = 4.64DD587 pKa = 3.24VHH589 pKa = 5.82TAA591 pKa = 2.97

Molecular weight:
67.92 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|W6EJ73|W6EJ73_9REOV Polyhedrin OS=Inachis io cypovirus 2 OX=1382295 PE=4 SV=1
MM1 pKa = 7.44EE2 pKa = 5.88LVPLTASNAEE12 pKa = 4.25LMLKK16 pKa = 8.49TYY18 pKa = 10.41CLAVRR23 pKa = 11.84PRR25 pKa = 11.84GKK27 pKa = 10.13KK28 pKa = 8.67GQPNKK33 pKa = 10.2VSTLKK38 pKa = 10.57IPNSYY43 pKa = 10.68YY44 pKa = 10.71KK45 pKa = 10.68GIEE48 pKa = 3.98KK49 pKa = 10.18PSYY52 pKa = 8.68TKK54 pKa = 10.54NVPQLPIQVLSIEE67 pKa = 4.5TQLKK71 pKa = 9.19VHH73 pKa = 6.91DD74 pKa = 3.81KK75 pKa = 10.36LRR77 pKa = 11.84RR78 pKa = 11.84IFEE81 pKa = 4.39RR82 pKa = 11.84NKK84 pKa = 10.3KK85 pKa = 10.07SYY87 pKa = 10.63EE88 pKa = 3.9NPRR91 pKa = 11.84LIARR95 pKa = 11.84LLTFGLTPKK104 pKa = 10.78GKK106 pKa = 9.13IDD108 pKa = 3.45MKK110 pKa = 11.28AFIDD114 pKa = 4.9SKK116 pKa = 11.15DD117 pKa = 3.15KK118 pKa = 11.01RR119 pKa = 11.84QNDD122 pKa = 3.63NKK124 pKa = 10.89DD125 pKa = 3.39VGPGRR130 pKa = 11.84SKK132 pKa = 10.74KK133 pKa = 9.9PKK135 pKa = 10.45LFGADD140 pKa = 3.13KK141 pKa = 11.06QDD143 pKa = 3.79FKK145 pKa = 11.64VKK147 pKa = 10.27KK148 pKa = 9.42HH149 pKa = 6.45DD150 pKa = 3.71VEE152 pKa = 4.5QSINDD157 pKa = 3.78DD158 pKa = 3.38FDD160 pKa = 4.72INAVHH165 pKa = 6.44SVDD168 pKa = 3.47GVRR171 pKa = 11.84DD172 pKa = 3.81LLEE175 pKa = 5.91LDD177 pKa = 4.59DD178 pKa = 5.33LRR180 pKa = 11.84GPLGITVVYY189 pKa = 8.73MINHH193 pKa = 6.7LFYY196 pKa = 10.52RR197 pKa = 11.84SKK199 pKa = 10.44IPAYY203 pKa = 7.27MAPLIPHH210 pKa = 6.62LFIRR214 pKa = 11.84KK215 pKa = 9.1INVNEE220 pKa = 3.94NMIPPLDD227 pKa = 3.59EE228 pKa = 5.89RR229 pKa = 11.84YY230 pKa = 9.28LQKK233 pKa = 10.37IYY235 pKa = 11.0LPVYY239 pKa = 7.49TASNILSCSIEE250 pKa = 4.22SSVEE254 pKa = 3.78SCIIAIDD261 pKa = 3.97KK262 pKa = 10.42QLSLADD268 pKa = 3.32KK269 pKa = 10.81QYY271 pKa = 11.01FDD273 pKa = 4.08KK274 pKa = 11.25HH275 pKa = 3.97FTNYY279 pKa = 9.94RR280 pKa = 11.84MIEE283 pKa = 4.1PGCALLMYY291 pKa = 10.54RR292 pKa = 11.84NYY294 pKa = 10.61TNNEE298 pKa = 4.08LCWIMSGLMLIEE310 pKa = 3.85AHH312 pKa = 6.89KK313 pKa = 10.46MIGKK317 pKa = 6.32TAKK320 pKa = 10.06EE321 pKa = 4.26SFNTLYY327 pKa = 9.42THH329 pKa = 7.63AYY331 pKa = 6.13EE332 pKa = 4.33TTVDD336 pKa = 3.38RR337 pKa = 11.84RR338 pKa = 11.84IVGGMKK344 pKa = 10.17VFIEE348 pKa = 3.97PTKK351 pKa = 10.53NYY353 pKa = 9.29VLEE356 pKa = 4.2RR357 pKa = 11.84VFPSGNPNVNPIILLRR373 pKa = 11.84TRR375 pKa = 11.84EE376 pKa = 3.7FFEE379 pKa = 4.05RR380 pKa = 11.84VKK382 pKa = 10.88LRR384 pKa = 11.84EE385 pKa = 3.92EE386 pKa = 4.49GKK388 pKa = 8.58FTGMNIYY395 pKa = 10.54GIIANKK401 pKa = 8.62GTGKK405 pKa = 8.44PTLSRR410 pKa = 11.84MLVEE414 pKa = 3.94RR415 pKa = 11.84LEE417 pKa = 4.19NKK419 pKa = 10.5GIGLSVIDD427 pKa = 3.53SDD429 pKa = 5.16EE430 pKa = 4.12YY431 pKa = 11.46GRR433 pKa = 11.84WLTMLLEE440 pKa = 4.41SSLINEE446 pKa = 4.86DD447 pKa = 4.12FSITEE452 pKa = 4.39PLDD455 pKa = 3.19EE456 pKa = 4.86YY457 pKa = 11.66YY458 pKa = 10.14PGRR461 pKa = 11.84PKK463 pKa = 10.66FKK465 pKa = 10.31LSEE468 pKa = 3.72RR469 pKa = 11.84SFFNIIMCMLLKK481 pKa = 10.32KK482 pKa = 10.16HH483 pKa = 6.62SITSRR488 pKa = 11.84DD489 pKa = 3.66KK490 pKa = 10.87LTLSYY495 pKa = 10.14YY496 pKa = 6.43TTRR499 pKa = 11.84CEE501 pKa = 4.93EE502 pKa = 3.89LFNDD506 pKa = 3.7YY507 pKa = 10.89KK508 pKa = 11.17EE509 pKa = 4.24YY510 pKa = 10.15LQKK513 pKa = 10.83VYY515 pKa = 9.75STEE518 pKa = 4.17SYY520 pKa = 11.55SMQAYY525 pKa = 9.42YY526 pKa = 10.6DD527 pKa = 3.57HH528 pKa = 7.67RR529 pKa = 11.84LPQCEE534 pKa = 4.15HH535 pKa = 6.43KK536 pKa = 10.96NIIVEE541 pKa = 4.2CHH543 pKa = 4.32TTLDD547 pKa = 4.02NVRR550 pKa = 11.84STPSDD555 pKa = 3.37LFVRR559 pKa = 11.84LGAFFDD565 pKa = 4.04PVVTITLIRR574 pKa = 11.84PSAKK578 pKa = 8.66EE579 pKa = 3.61TAKK582 pKa = 10.27TGLFHH587 pKa = 8.11LGEE590 pKa = 4.32LALYY594 pKa = 9.73YY595 pKa = 10.34YY596 pKa = 10.77YY597 pKa = 9.93EE598 pKa = 4.34TATVPDD604 pKa = 3.64VALVYY609 pKa = 9.86PSNVLYY615 pKa = 10.85ALDD618 pKa = 4.46LEE620 pKa = 4.9LGKK623 pKa = 8.78TPDD626 pKa = 3.13IVQVSSEE633 pKa = 3.88

Molecular weight:
72.87 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

7389

274

1225

738.9

83.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.968 ± 0.327

1.164 ± 0.148

6.009 ± 0.247

5.305 ± 0.277

4.629 ± 0.318

4.98 ± 0.192

2.477 ± 0.188

7.484 ± 0.297

4.913 ± 0.669

8.932 ± 0.485

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.666 ± 0.199

6.401 ± 0.37

4.453 ± 0.256

3.451 ± 0.194

5.265 ± 0.234

7.498 ± 0.631

7.065 ± 0.403

5.968 ± 0.282

0.717 ± 0.145

4.656 ± 0.473

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski