Inachis io cypovirus 2
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W6EJ67|W6EJ67_9REOV VP5 OS=Inachis io cypovirus 2 OX=1382295 PE=4 SV=1
MM1 pKa = 8.0 AYY3 pKa = 8.99 PAQGYY8 pKa = 9.09 VLDD11 pKa = 3.76 EE12 pKa = 4.34 RR13 pKa = 11.84 FNFEE17 pKa = 3.91 YY18 pKa = 10.34 AIKK21 pKa = 10.42 LLRR24 pKa = 11.84 EE25 pKa = 3.54 ADD27 pKa = 3.44 INGEE31 pKa = 4.26 TKK33 pKa = 10.76 DD34 pKa = 3.62 KK35 pKa = 11.41 VSVAEE40 pKa = 4.37 LRR42 pKa = 11.84 DD43 pKa = 4.31 FIIKK47 pKa = 9.43 EE48 pKa = 4.0 AQDD51 pKa = 3.12 EE52 pKa = 4.32 QFAVRR57 pKa = 11.84 EE58 pKa = 4.17 RR59 pKa = 11.84 PLPPAQVRR67 pKa = 11.84 GISARR72 pKa = 11.84 VTVCPINSILCQLFCGDD89 pKa = 3.61 GFYY92 pKa = 10.94 AQSFIKK98 pKa = 10.53 LMAIGQMYY106 pKa = 10.32 ANQITAEE113 pKa = 4.05 AFTVEE118 pKa = 3.99 EE119 pKa = 4.24 GQFYY123 pKa = 10.81 VNEE126 pKa = 4.41 GGIGPDD132 pKa = 3.02 STKK135 pKa = 10.72 YY136 pKa = 8.47 PTAHH140 pKa = 6.36 VAPLMEE146 pKa = 4.76 AFRR149 pKa = 11.84 RR150 pKa = 11.84 CANAMDD156 pKa = 4.26 STIDD160 pKa = 3.3 PHH162 pKa = 6.69 GTIQQLYY169 pKa = 9.69 ALMEE173 pKa = 4.41 EE174 pKa = 4.5 TTHH177 pKa = 5.8 MEE179 pKa = 4.08 TWFMDD184 pKa = 3.7 PSVITPRR191 pKa = 11.84 QITSSIQNWNLFAAFVDD208 pKa = 4.03 MSEE211 pKa = 4.36 PNPVFPVATWQKK223 pKa = 10.97 LLDD226 pKa = 3.53 ILRR229 pKa = 11.84 AVVLTFITSVSSTAVFDD246 pKa = 4.19 EE247 pKa = 5.09 GPPLQISFLLPSYY260 pKa = 10.75 QLTNPRR266 pKa = 11.84 LVNFTRR272 pKa = 11.84 SYY274 pKa = 10.9 QNLRR278 pKa = 11.84 QQYY281 pKa = 9.2 ISWILRR287 pKa = 11.84 TVFIPFLEE295 pKa = 3.97 RR296 pKa = 11.84 LRR298 pKa = 11.84 RR299 pKa = 11.84 FIVGNNARR307 pKa = 11.84 IARR310 pKa = 11.84 TTFAGEE316 pKa = 3.93 KK317 pKa = 9.74 KK318 pKa = 10.31 VAIPYY323 pKa = 10.25 FGDD326 pKa = 3.45 DD327 pKa = 4.96 RR328 pKa = 11.84 EE329 pKa = 4.38 MKK331 pKa = 9.69 TNNTIQVVDD340 pKa = 3.2 KK341 pKa = 10.71 RR342 pKa = 11.84 GRR344 pKa = 11.84 ANYY347 pKa = 9.51 DD348 pKa = 2.96 AYY350 pKa = 10.07 KK351 pKa = 9.42 YY352 pKa = 9.05 TLEE355 pKa = 3.78 NHH357 pKa = 6.61 LLFGEE362 pKa = 4.39 DD363 pKa = 2.93 GRR365 pKa = 11.84 RR366 pKa = 11.84 PRR368 pKa = 11.84 MFTKK372 pKa = 10.32 TLVQNDD378 pKa = 4.17 DD379 pKa = 3.41 LSTFHH384 pKa = 6.46 VQIFNSFTDD393 pKa = 4.3 TIYY396 pKa = 9.67 GTNKK400 pKa = 9.98 NVTAAYY406 pKa = 8.25 DD407 pKa = 3.53 TMSALVAKK415 pKa = 7.24 YY416 pKa = 8.85 TPINYY421 pKa = 7.54 NTLIVEE427 pKa = 4.37 SLDD430 pKa = 3.44 DD431 pKa = 3.83 YY432 pKa = 12.01 NRR434 pKa = 11.84 INLKK438 pKa = 9.47 TGDD441 pKa = 3.55 ISQVAVWFLTEE452 pKa = 3.37 YY453 pKa = 10.4 SRR455 pKa = 11.84 PYY457 pKa = 10.65 VYY459 pKa = 10.52 FGVASLYY466 pKa = 11.19 LLDD469 pKa = 4.47 DD470 pKa = 4.37 TIIAYY475 pKa = 7.47 TVHH478 pKa = 6.19 TNVGRR483 pKa = 11.84 YY484 pKa = 9.0 HH485 pKa = 6.68 EE486 pKa = 4.67 GEE488 pKa = 3.8 KK489 pKa = 10.06 TFAFEE494 pKa = 4.13 EE495 pKa = 4.7 CTAMHH500 pKa = 7.05 MFNILYY506 pKa = 10.03 SLQVCLDD513 pKa = 3.55 PEE515 pKa = 4.31 AFLTNGEE522 pKa = 4.19 MDD524 pKa = 3.17 KK525 pKa = 11.25 HH526 pKa = 6.68 EE527 pKa = 4.29 YY528 pKa = 8.7 TLDD531 pKa = 3.39 YY532 pKa = 10.6 RR533 pKa = 11.84 VWRR536 pKa = 11.84 EE537 pKa = 3.69 ALSQEE542 pKa = 5.47 LKK544 pKa = 9.01 TCWRR548 pKa = 11.84 PLCDD552 pKa = 3.06 HH553 pKa = 6.11 YY554 pKa = 11.72 ARR556 pKa = 11.84 RR557 pKa = 11.84 NVQDD561 pKa = 3.59 TPTVAMAEE569 pKa = 4.35 MKK571 pKa = 10.02 EE572 pKa = 4.05 LMGAYY577 pKa = 9.05 PYY579 pKa = 9.37 TAIYY583 pKa = 9.94 KK584 pKa = 10.41 FEE586 pKa = 4.64 DD587 pKa = 3.24 VHH589 pKa = 5.82 TAA591 pKa = 2.97
Molecular weight: 67.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.09
IPC2_protein 5.207
IPC_protein 5.181
Toseland 5.194
ProMoST 5.359
Dawson 5.219
Bjellqvist 5.321
Wikipedia 5.105
Rodwell 5.143
Grimsley 5.143
Solomon 5.219
Lehninger 5.181
Nozaki 5.359
DTASelect 5.512
Thurlkill 5.219
EMBOSS 5.181
Sillero 5.436
Patrickios 3.63
IPC_peptide 5.219
IPC2_peptide 5.436
IPC2.peptide.svr19 5.349
Protein with the highest isoelectric point:
>tr|W6EJ73|W6EJ73_9REOV Polyhedrin OS=Inachis io cypovirus 2 OX=1382295 PE=4 SV=1
MM1 pKa = 7.44 EE2 pKa = 5.88 LVPLTASNAEE12 pKa = 4.25 LMLKK16 pKa = 8.49 TYY18 pKa = 10.41 CLAVRR23 pKa = 11.84 PRR25 pKa = 11.84 GKK27 pKa = 10.13 KK28 pKa = 8.67 GQPNKK33 pKa = 10.2 VSTLKK38 pKa = 10.57 IPNSYY43 pKa = 10.68 YY44 pKa = 10.71 KK45 pKa = 10.68 GIEE48 pKa = 3.98 KK49 pKa = 10.18 PSYY52 pKa = 8.68 TKK54 pKa = 10.54 NVPQLPIQVLSIEE67 pKa = 4.5 TQLKK71 pKa = 9.19 VHH73 pKa = 6.91 DD74 pKa = 3.81 KK75 pKa = 10.36 LRR77 pKa = 11.84 RR78 pKa = 11.84 IFEE81 pKa = 4.39 RR82 pKa = 11.84 NKK84 pKa = 10.3 KK85 pKa = 10.07 SYY87 pKa = 10.63 EE88 pKa = 3.9 NPRR91 pKa = 11.84 LIARR95 pKa = 11.84 LLTFGLTPKK104 pKa = 10.78 GKK106 pKa = 9.13 IDD108 pKa = 3.45 MKK110 pKa = 11.28 AFIDD114 pKa = 4.9 SKK116 pKa = 11.15 DD117 pKa = 3.15 KK118 pKa = 11.01 RR119 pKa = 11.84 QNDD122 pKa = 3.63 NKK124 pKa = 10.89 DD125 pKa = 3.39 VGPGRR130 pKa = 11.84 SKK132 pKa = 10.74 KK133 pKa = 9.9 PKK135 pKa = 10.45 LFGADD140 pKa = 3.13 KK141 pKa = 11.06 QDD143 pKa = 3.79 FKK145 pKa = 11.64 VKK147 pKa = 10.27 KK148 pKa = 9.42 HH149 pKa = 6.45 DD150 pKa = 3.71 VEE152 pKa = 4.5 QSINDD157 pKa = 3.78 DD158 pKa = 3.38 FDD160 pKa = 4.72 INAVHH165 pKa = 6.44 SVDD168 pKa = 3.47 GVRR171 pKa = 11.84 DD172 pKa = 3.81 LLEE175 pKa = 5.91 LDD177 pKa = 4.59 DD178 pKa = 5.33 LRR180 pKa = 11.84 GPLGITVVYY189 pKa = 8.73 MINHH193 pKa = 6.7 LFYY196 pKa = 10.52 RR197 pKa = 11.84 SKK199 pKa = 10.44 IPAYY203 pKa = 7.27 MAPLIPHH210 pKa = 6.62 LFIRR214 pKa = 11.84 KK215 pKa = 9.1 INVNEE220 pKa = 3.94 NMIPPLDD227 pKa = 3.59 EE228 pKa = 5.89 RR229 pKa = 11.84 YY230 pKa = 9.28 LQKK233 pKa = 10.37 IYY235 pKa = 11.0 LPVYY239 pKa = 7.49 TASNILSCSIEE250 pKa = 4.22 SSVEE254 pKa = 3.78 SCIIAIDD261 pKa = 3.97 KK262 pKa = 10.42 QLSLADD268 pKa = 3.32 KK269 pKa = 10.81 QYY271 pKa = 11.01 FDD273 pKa = 4.08 KK274 pKa = 11.25 HH275 pKa = 3.97 FTNYY279 pKa = 9.94 RR280 pKa = 11.84 MIEE283 pKa = 4.1 PGCALLMYY291 pKa = 10.54 RR292 pKa = 11.84 NYY294 pKa = 10.61 TNNEE298 pKa = 4.08 LCWIMSGLMLIEE310 pKa = 3.85 AHH312 pKa = 6.89 KK313 pKa = 10.46 MIGKK317 pKa = 6.32 TAKK320 pKa = 10.06 EE321 pKa = 4.26 SFNTLYY327 pKa = 9.42 THH329 pKa = 7.63 AYY331 pKa = 6.13 EE332 pKa = 4.33 TTVDD336 pKa = 3.38 RR337 pKa = 11.84 RR338 pKa = 11.84 IVGGMKK344 pKa = 10.17 VFIEE348 pKa = 3.97 PTKK351 pKa = 10.53 NYY353 pKa = 9.29 VLEE356 pKa = 4.2 RR357 pKa = 11.84 VFPSGNPNVNPIILLRR373 pKa = 11.84 TRR375 pKa = 11.84 EE376 pKa = 3.7 FFEE379 pKa = 4.05 RR380 pKa = 11.84 VKK382 pKa = 10.88 LRR384 pKa = 11.84 EE385 pKa = 3.92 EE386 pKa = 4.49 GKK388 pKa = 8.58 FTGMNIYY395 pKa = 10.54 GIIANKK401 pKa = 8.62 GTGKK405 pKa = 8.44 PTLSRR410 pKa = 11.84 MLVEE414 pKa = 3.94 RR415 pKa = 11.84 LEE417 pKa = 4.19 NKK419 pKa = 10.5 GIGLSVIDD427 pKa = 3.53 SDD429 pKa = 5.16 EE430 pKa = 4.12 YY431 pKa = 11.46 GRR433 pKa = 11.84 WLTMLLEE440 pKa = 4.41 SSLINEE446 pKa = 4.86 DD447 pKa = 4.12 FSITEE452 pKa = 4.39 PLDD455 pKa = 3.19 EE456 pKa = 4.86 YY457 pKa = 11.66 YY458 pKa = 10.14 PGRR461 pKa = 11.84 PKK463 pKa = 10.66 FKK465 pKa = 10.31 LSEE468 pKa = 3.72 RR469 pKa = 11.84 SFFNIIMCMLLKK481 pKa = 10.32 KK482 pKa = 10.16 HH483 pKa = 6.62 SITSRR488 pKa = 11.84 DD489 pKa = 3.66 KK490 pKa = 10.87 LTLSYY495 pKa = 10.14 YY496 pKa = 6.43 TTRR499 pKa = 11.84 CEE501 pKa = 4.93 EE502 pKa = 3.89 LFNDD506 pKa = 3.7 YY507 pKa = 10.89 KK508 pKa = 11.17 EE509 pKa = 4.24 YY510 pKa = 10.15 LQKK513 pKa = 10.83 VYY515 pKa = 9.75 STEE518 pKa = 4.17 SYY520 pKa = 11.55 SMQAYY525 pKa = 9.42 YY526 pKa = 10.6 DD527 pKa = 3.57 HH528 pKa = 7.67 RR529 pKa = 11.84 LPQCEE534 pKa = 4.15 HH535 pKa = 6.43 KK536 pKa = 10.96 NIIVEE541 pKa = 4.2 CHH543 pKa = 4.32 TTLDD547 pKa = 4.02 NVRR550 pKa = 11.84 STPSDD555 pKa = 3.37 LFVRR559 pKa = 11.84 LGAFFDD565 pKa = 4.04 PVVTITLIRR574 pKa = 11.84 PSAKK578 pKa = 8.66 EE579 pKa = 3.61 TAKK582 pKa = 10.27 TGLFHH587 pKa = 8.11 LGEE590 pKa = 4.32 LALYY594 pKa = 9.73 YY595 pKa = 10.34 YY596 pKa = 10.77 YY597 pKa = 9.93 EE598 pKa = 4.34 TATVPDD604 pKa = 3.64 VALVYY609 pKa = 9.86 PSNVLYY615 pKa = 10.85 ALDD618 pKa = 4.46 LEE620 pKa = 4.9 LGKK623 pKa = 8.78 TPDD626 pKa = 3.13 IVQVSSEE633 pKa = 3.88
Molecular weight: 72.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.888
IPC2_protein 8.083
IPC_protein 7.936
Toseland 8.141
ProMoST 8.448
Dawson 8.77
Bjellqvist 8.814
Wikipedia 8.829
Rodwell 8.843
Grimsley 8.317
Solomon 8.829
Lehninger 8.799
Nozaki 8.887
DTASelect 8.668
Thurlkill 8.741
EMBOSS 8.916
Sillero 8.96
Patrickios 4.279
IPC_peptide 8.814
IPC2_peptide 7.527
IPC2.peptide.svr19 7.715
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
7389
274
1225
738.9
83.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.968 ± 0.327
1.164 ± 0.148
6.009 ± 0.247
5.305 ± 0.277
4.629 ± 0.318
4.98 ± 0.192
2.477 ± 0.188
7.484 ± 0.297
4.913 ± 0.669
8.932 ± 0.485
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.666 ± 0.199
6.401 ± 0.37
4.453 ± 0.256
3.451 ± 0.194
5.265 ± 0.234
7.498 ± 0.631
7.065 ± 0.403
5.968 ± 0.282
0.717 ± 0.145
4.656 ± 0.473
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here