Aotine betaherpesvirus 1
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 146 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G8XU82|G8XU82_9BETA Protein A9 OS=Aotine betaherpesvirus 1 OX=50290 GN=A9 PE=4 SV=1
MM1 pKa = 7.9 AEE3 pKa = 4.29 PGEE6 pKa = 4.09 PSEE9 pKa = 4.46 PGEE12 pKa = 4.3 PNTPLRR18 pKa = 11.84 NAEE21 pKa = 4.1 PQLDD25 pKa = 4.01 FQSPHH30 pKa = 4.36 QQGNEE35 pKa = 3.76 DD36 pKa = 3.65 TEE38 pKa = 4.31 PRR40 pKa = 11.84 NKK42 pKa = 10.39 CPVLLLMLLLLPLFIQILAMIITFILSNQNPIAAGICSVMFLLSAISTAIIICNVLTYY100 pKa = 10.72 GYY102 pKa = 10.48 FF103 pKa = 3.34
Molecular weight: 11.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.126
IPC2_protein 4.431
IPC_protein 4.202
Toseland 4.062
ProMoST 4.304
Dawson 4.126
Bjellqvist 4.291
Wikipedia 3.999
Rodwell 4.05
Grimsley 3.986
Solomon 4.113
Lehninger 4.062
Nozaki 4.266
DTASelect 4.317
Thurlkill 4.088
EMBOSS 4.012
Sillero 4.304
Patrickios 0.693
IPC_peptide 4.126
IPC2_peptide 4.291
IPC2.peptide.svr19 4.226
Protein with the highest isoelectric point:
>tr|G8XUH6|G8XUH6_9BETA Membrane glycoprotein UL119 OS=Aotine betaherpesvirus 1 OX=50290 GN=UL119 PE=4 SV=1
MM1 pKa = 7.16 FSKK4 pKa = 11.11 VEE6 pKa = 4.22 IIRR9 pKa = 11.84 VRR11 pKa = 11.84 EE12 pKa = 4.06 PIKK15 pKa = 10.99 LNSEE19 pKa = 4.15 SLAPANKK26 pKa = 9.63 RR27 pKa = 11.84 KK28 pKa = 9.85 RR29 pKa = 11.84 NRR31 pKa = 11.84 AIVISPQTQGFVKK44 pKa = 10.45 IPPDD48 pKa = 3.48 NKK50 pKa = 9.99 NVNSVRR56 pKa = 11.84 IVGSRR61 pKa = 11.84 PARR64 pKa = 11.84 RR65 pKa = 11.84 PSPLQPPQPRR75 pKa = 11.84 HH76 pKa = 4.46 QQPRR80 pKa = 11.84 TYY82 pKa = 10.19 PYY84 pKa = 8.21 GTSLVRR90 pKa = 11.84 RR91 pKa = 11.84 RR92 pKa = 11.84 RR93 pKa = 11.84 IRR95 pKa = 11.84 PNGAQHH101 pKa = 7.27 LIQRR105 pKa = 11.84 YY106 pKa = 6.85 GHH108 pKa = 6.46 ILEE111 pKa = 4.33 EE112 pKa = 4.17 LARR115 pKa = 11.84 LLQMYY120 pKa = 10.16 DD121 pKa = 3.03 STAVQLQVNSLCGNSFNQLKK141 pKa = 8.17 TAVLKK146 pKa = 10.12 LQNVTVLTGMQMATQCLPHH165 pKa = 7.32 DD166 pKa = 4.16 ARR168 pKa = 11.84 AVSTFKK174 pKa = 10.38 HH175 pKa = 5.94 HH176 pKa = 6.06 EE177 pKa = 3.86 RR178 pKa = 11.84 LGRR181 pKa = 11.84 FMDD184 pKa = 3.75 NVAPVLPRR192 pKa = 11.84 SSCYY196 pKa = 10.79 ADD198 pKa = 2.84 TGLYY202 pKa = 9.32 QMCISGATRR211 pKa = 11.84 KK212 pKa = 10.35 DD213 pKa = 3.49 LVDD216 pKa = 3.4 ATTLCISIIDD226 pKa = 3.57 QRR228 pKa = 11.84 PNVFNINIAKK238 pKa = 10.46 LSYY241 pKa = 9.7 PSLAGLHH248 pKa = 6.32 LGTYY252 pKa = 10.35 NEE254 pKa = 4.31 FSNTVVAA261 pKa = 5.46
Molecular weight: 29.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 9.721
IPC_protein 10.482
Toseland 10.54
ProMoST 10.248
Dawson 10.672
Bjellqvist 10.409
Wikipedia 10.891
Rodwell 10.906
Grimsley 10.745
Solomon 10.76
Lehninger 10.73
Nozaki 10.54
DTASelect 10.394
Thurlkill 10.555
EMBOSS 10.935
Sillero 10.613
Patrickios 10.584
IPC_peptide 10.76
IPC2_peptide 9.516
IPC2.peptide.svr19 8.506
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
146
0
146
56898
60
2158
389.7
43.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.724 ± 0.364
2.54 ± 0.116
4.981 ± 0.13
5.338 ± 0.171
4.123 ± 0.12
5.44 ± 0.181
2.805 ± 0.096
4.425 ± 0.158
3.71 ± 0.159
9.999 ± 0.173
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.332 ± 0.094
3.87 ± 0.175
5.515 ± 0.235
3.573 ± 0.121
6.796 ± 0.19
7.524 ± 0.192
6.84 ± 0.322
7.589 ± 0.221
1.25 ± 0.087
3.626 ± 0.105
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here