Helicocarpus griseus UAMH5409

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales; Ajellomycetaceae; Helicocarpus; Helicocarpus griseus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10216 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2B7WNJ9|A0A2B7WNJ9_9EURO Ribose-phosphate diphosphokinase OS=Helicocarpus griseus UAMH5409 OX=1447875 GN=AJ79_08981 PE=3 SV=1
MM1 pKa = 7.83ADD3 pKa = 3.95DD4 pKa = 4.82ALSIYY9 pKa = 10.64DD10 pKa = 4.0EE11 pKa = 4.57IEE13 pKa = 4.49IEE15 pKa = 4.88DD16 pKa = 3.73MTYY19 pKa = 11.23DD20 pKa = 3.5PTLQIYY26 pKa = 9.13HH27 pKa = 5.87YY28 pKa = 8.86PCPCGDD34 pKa = 3.29RR35 pKa = 11.84FEE37 pKa = 5.81IGIADD42 pKa = 3.73LRR44 pKa = 11.84DD45 pKa = 3.61GEE47 pKa = 4.69EE48 pKa = 3.84IAVCPSCSLMVRR60 pKa = 11.84VIFDD64 pKa = 4.76LDD66 pKa = 4.27DD67 pKa = 4.1LPKK70 pKa = 10.94DD71 pKa = 3.76DD72 pKa = 5.99DD73 pKa = 4.43GAGKK77 pKa = 10.28GAVAVTAA84 pKa = 4.44

Molecular weight: 9.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2B7XV12|A0A2B7XV12_9EURO Uncharacterized protein OS=Helicocarpus griseus UAMH5409 OX=1447875 GN=AJ79_03883 PE=3 SV=1
MM1 pKa = 7.45EE2 pKa = 5.49ISWAIIGRR10 pKa = 11.84ITRR13 pKa = 11.84PRR15 pKa = 11.84KK16 pKa = 8.15VQEE19 pKa = 3.62GTRR22 pKa = 11.84DD23 pKa = 3.46VKK25 pKa = 10.99RR26 pKa = 11.84KK27 pKa = 7.25ITRR30 pKa = 11.84TLILKK35 pKa = 9.4LEE37 pKa = 4.29PVAATQRR44 pKa = 11.84AEE46 pKa = 4.04TRR48 pKa = 11.84GPLSLDD54 pKa = 3.56LFRR57 pKa = 11.84PGLARR62 pKa = 11.84RR63 pKa = 11.84ASWEE67 pKa = 4.07PQMHH71 pKa = 6.77VYY73 pKa = 10.1PMPGRR78 pKa = 11.84GVQSLLCFKK87 pKa = 10.84LQTDD91 pKa = 4.8DD92 pKa = 3.56KK93 pKa = 10.61ASPYY97 pKa = 9.69FLEE100 pKa = 5.49PIRR103 pKa = 11.84LCWGSLRR110 pKa = 11.84PWDD113 pKa = 3.96HH114 pKa = 5.85THH116 pKa = 5.93LTGPVTRR123 pKa = 11.84PGDD126 pKa = 3.85QNHH129 pKa = 6.29HH130 pKa = 5.46RR131 pKa = 11.84TFF133 pKa = 3.66

Molecular weight: 15.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10216

0

10216

4942617

32

10649

483.8

53.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.209 ± 0.023

1.212 ± 0.009

5.673 ± 0.015

6.327 ± 0.022

3.697 ± 0.017

6.939 ± 0.024

2.378 ± 0.01

4.943 ± 0.015

5.07 ± 0.023

8.787 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.09 ± 0.009

3.892 ± 0.012

6.273 ± 0.028

3.984 ± 0.018

6.19 ± 0.023

8.442 ± 0.031

5.815 ± 0.016

5.93 ± 0.015

1.375 ± 0.009

2.773 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski