Saccharibacillus brassicae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Saccharibacillus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4543 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6UZP1|A0A4Y6UZP1_9BACL 3-oxoacyl-[acyl-carrier-protein] synthase 2 OS=Saccharibacillus brassicae OX=2583377 GN=fabF PE=3 SV=1
MM1 pKa = 8.14RR2 pKa = 11.84LRR4 pKa = 11.84IIAGCLAFSVVVATGCSSVSTDD26 pKa = 2.52ADD28 pKa = 3.53VRR30 pKa = 11.84HH31 pKa = 5.95EE32 pKa = 4.13ADD34 pKa = 3.56KK35 pKa = 11.2AVQTTSDD42 pKa = 3.54QLNNLANLADD52 pKa = 3.68EE53 pKa = 5.51HH54 pKa = 8.5IGFVKK59 pKa = 10.52NGTMNNRR66 pKa = 11.84DD67 pKa = 3.95DD68 pKa = 3.8VTLGEE73 pKa = 4.2VMDD76 pKa = 5.02AFFDD80 pKa = 4.42GPTWQYY86 pKa = 11.29FSGTNDD92 pKa = 3.19EE93 pKa = 4.59TGDD96 pKa = 3.6TFDD99 pKa = 3.45VVEE102 pKa = 4.25FTGYY106 pKa = 10.57FLYY109 pKa = 10.84NEE111 pKa = 4.4KK112 pKa = 10.25SAKK115 pKa = 10.19ARR117 pKa = 11.84IQFILHH123 pKa = 6.65EE124 pKa = 5.28DD125 pKa = 3.45DD126 pKa = 3.59TFEE129 pKa = 4.38MGVGSYY135 pKa = 11.38NDD137 pKa = 3.32IDD139 pKa = 3.69QTALVLSLLMDD150 pKa = 4.08KK151 pKa = 10.84VYY153 pKa = 10.95EE154 pKa = 4.47SYY156 pKa = 11.18DD157 pKa = 3.52EE158 pKa = 3.99EE159 pKa = 4.85HH160 pKa = 6.85AVSTADD166 pKa = 3.29QAQAGGEE173 pKa = 4.2PVTADD178 pKa = 3.06SSQIQAAKK186 pKa = 10.75AEE188 pKa = 4.88GIGLEE193 pKa = 4.44NFYY196 pKa = 10.93KK197 pKa = 8.54WTPGEE202 pKa = 3.94NDD204 pKa = 2.93RR205 pKa = 11.84SFPLMLDD212 pKa = 3.29GEE214 pKa = 4.63EE215 pKa = 4.07IEE217 pKa = 4.76FVVGRR222 pKa = 11.84NTPNGIKK229 pKa = 8.93MQVFSSSMEE238 pKa = 4.01QGWQLPTEE246 pKa = 4.39LADD249 pKa = 4.18GSIGPFNDD257 pKa = 3.3YY258 pKa = 11.31GDD260 pKa = 4.78LIEE263 pKa = 5.62GFSLYY268 pKa = 10.54VKK270 pKa = 10.05EE271 pKa = 4.48YY272 pKa = 10.83DD273 pKa = 3.62FASDD277 pKa = 3.92GVPEE281 pKa = 4.34VVLVASDD288 pKa = 4.0GMLEE292 pKa = 4.22SYY294 pKa = 10.29VWVYY298 pKa = 10.87NYY300 pKa = 10.78NYY302 pKa = 9.37TFSEE306 pKa = 4.47YY307 pKa = 10.53DD308 pKa = 3.37VSPLEE313 pKa = 3.97LVWYY317 pKa = 10.57GEE319 pKa = 4.41GQSDD323 pKa = 3.96VQLEE327 pKa = 4.08GDD329 pKa = 4.54RR330 pKa = 11.84IVLPYY335 pKa = 10.41GSQGLYY341 pKa = 10.36EE342 pKa = 4.14EE343 pKa = 4.57YY344 pKa = 10.64VYY346 pKa = 11.29KK347 pKa = 10.64NEE349 pKa = 5.37KK350 pKa = 9.91FIQQ353 pKa = 3.6

Molecular weight: 39.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6UW26|A0A4Y6UW26_9BACL Uncharacterized protein OS=Saccharibacillus brassicae OX=2583377 GN=FFV09_04625 PE=4 SV=1
MM1 pKa = 7.61GPTFKK6 pKa = 10.88PNTRR10 pKa = 11.84KK11 pKa = 9.83RR12 pKa = 11.84KK13 pKa = 8.74KK14 pKa = 8.74VHH16 pKa = 5.61GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4543

0

4543

1557127

26

3974

342.8

37.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.924 ± 0.049

0.677 ± 0.009

5.429 ± 0.025

7.204 ± 0.044

3.867 ± 0.025

8.206 ± 0.038

1.928 ± 0.016

5.566 ± 0.032

4.291 ± 0.039

10.217 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.535 ± 0.019

3.204 ± 0.024

4.4 ± 0.028

3.545 ± 0.023

6.274 ± 0.041

6.141 ± 0.031

5.278 ± 0.037

6.9 ± 0.034

1.178 ± 0.015

3.236 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski