Amino acid dipepetide frequency for Saccharibacillus brassicae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.522AlaAla: 13.522 ± 0.172
0.783AlaCys: 0.783 ± 0.024
6.114AlaAsp: 6.114 ± 0.076
7.712AlaGlu: 7.712 ± 0.098
3.707AlaPhe: 3.707 ± 0.054
9.284AlaGly: 9.284 ± 0.103
1.57AlaHis: 1.57 ± 0.03
4.743AlaIle: 4.743 ± 0.066
3.845AlaLys: 3.845 ± 0.054
10.0AlaLeu: 10.0 ± 0.099
2.27AlaMet: 2.27 ± 0.045
2.629AlaAsn: 2.629 ± 0.04
3.748AlaPro: 3.748 ± 0.069
3.579AlaGln: 3.579 ± 0.057
4.996AlaArg: 4.996 ± 0.069
6.211AlaSer: 6.211 ± 0.074
3.352AlaThr: 3.352 ± 0.059
7.046AlaVal: 7.046 ± 0.083
1.053AlaTrp: 1.053 ± 0.029
3.079AlaTyr: 3.079 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.613CysAla: 0.613 ± 0.022
0.094CysCys: 0.094 ± 0.008
0.383CysAsp: 0.383 ± 0.016
0.437CysGlu: 0.437 ± 0.018
0.258CysPhe: 0.258 ± 0.013
0.76CysGly: 0.76 ± 0.024
0.144CysHis: 0.144 ± 0.01
0.375CysIle: 0.375 ± 0.016
0.22CysLys: 0.22 ± 0.013
0.627CysLeu: 0.627 ± 0.02
0.171CysMet: 0.171 ± 0.01
0.168CysAsn: 0.168 ± 0.011
0.319CysPro: 0.319 ± 0.016
0.153CysGln: 0.153 ± 0.011
0.507CysArg: 0.507 ± 0.019
0.446CysSer: 0.446 ± 0.017
0.369CysThr: 0.369 ± 0.015
0.433CysVal: 0.433 ± 0.017
0.071CysTrp: 0.071 ± 0.007
0.219CysTyr: 0.219 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
4.987AspAla: 4.987 ± 0.062
0.372AspCys: 0.372 ± 0.016
2.583AspAsp: 2.583 ± 0.046
4.173AspGlu: 4.173 ± 0.057
2.108AspPhe: 2.108 ± 0.041
4.686AspGly: 4.686 ± 0.068
1.008AspHis: 1.008 ± 0.026
2.885AspIle: 2.885 ± 0.046
2.374AspLys: 2.374 ± 0.045
5.39AspLeu: 5.39 ± 0.055
1.323AspMet: 1.323 ± 0.03
1.545AspAsn: 1.545 ± 0.039
2.901AspPro: 2.901 ± 0.048
1.541AspGln: 1.541 ± 0.034
3.853AspArg: 3.853 ± 0.055
2.893AspSer: 2.893 ± 0.041
2.85AspThr: 2.85 ± 0.05
3.749AspVal: 3.749 ± 0.053
0.863AspTrp: 0.863 ± 0.025
2.2AspTyr: 2.2 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
7.923GluAla: 7.923 ± 0.098
0.358GluCys: 0.358 ± 0.018
3.484GluAsp: 3.484 ± 0.054
5.77GluGlu: 5.77 ± 0.08
2.203GluPhe: 2.203 ± 0.036
4.745GluGly: 4.745 ± 0.063
1.573GluHis: 1.573 ± 0.035
4.101GluIle: 4.101 ± 0.056
3.322GluLys: 3.322 ± 0.058
7.622GluLeu: 7.622 ± 0.097
1.817GluMet: 1.817 ± 0.038
2.325GluAsn: 2.325 ± 0.045
2.815GluPro: 2.815 ± 0.047
3.641GluGln: 3.641 ± 0.063
5.619GluArg: 5.619 ± 0.072
3.953GluSer: 3.953 ± 0.055
3.623GluThr: 3.623 ± 0.058
4.314GluVal: 4.314 ± 0.074
0.889GluTrp: 0.889 ± 0.032
1.943GluTyr: 1.943 ± 0.036
0.0GluXaa: 0.0 ± 0.0
Phe
3.878PheAla: 3.878 ± 0.058
0.334PheCys: 0.334 ± 0.017
2.512PheAsp: 2.512 ± 0.042
2.677PheGlu: 2.677 ± 0.044
1.667PhePhe: 1.667 ± 0.039
3.521PheGly: 3.521 ± 0.061
0.719PheHis: 0.719 ± 0.023
1.951PheIle: 1.951 ± 0.037
1.633PheLys: 1.633 ± 0.033
3.408PheLeu: 3.408 ± 0.056
0.989PheMet: 0.989 ± 0.028
1.279PheAsn: 1.279 ± 0.029
1.443PhePro: 1.443 ± 0.03
1.011PheGln: 1.011 ± 0.027
2.277PheArg: 2.277 ± 0.044
2.419PheSer: 2.419 ± 0.045
2.068PheThr: 2.068 ± 0.042
2.98PheVal: 2.98 ± 0.049
0.522PheTrp: 0.522 ± 0.018
1.384PheTyr: 1.384 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
7.227GlyAla: 7.227 ± 0.079
0.665GlyCys: 0.665 ± 0.023
4.118GlyAsp: 4.118 ± 0.055
6.032GlyGlu: 6.032 ± 0.067
3.265GlyPhe: 3.265 ± 0.044
7.105GlyGly: 7.105 ± 0.094
1.482GlyHis: 1.482 ± 0.034
5.054GlyIle: 5.054 ± 0.063
3.749GlyLys: 3.749 ± 0.062
7.941GlyLeu: 7.941 ± 0.086
2.381GlyMet: 2.381 ± 0.04
2.07GlyAsn: 2.07 ± 0.045
2.363GlyPro: 2.363 ± 0.043
2.886GlyGln: 2.886 ± 0.041
5.445GlyArg: 5.445 ± 0.08
5.782GlySer: 5.782 ± 0.068
5.077GlyThr: 5.077 ± 0.087
5.429GlyVal: 5.429 ± 0.073
1.109GlyTrp: 1.109 ± 0.034
2.885GlyTyr: 2.885 ± 0.043
0.0GlyXaa: 0.0 ± 0.0
His
1.835HisAla: 1.835 ± 0.036
0.155HisCys: 0.155 ± 0.01
1.016HisAsp: 1.016 ± 0.027
1.291HisGlu: 1.291 ± 0.026
0.88HisPhe: 0.88 ± 0.024
1.566HisGly: 1.566 ± 0.035
0.482HisHis: 0.482 ± 0.02
1.077HisIle: 1.077 ± 0.026
0.69HisLys: 0.69 ± 0.023
1.839HisLeu: 1.839 ± 0.036
0.489HisMet: 0.489 ± 0.015
0.598HisAsn: 0.598 ± 0.02
1.175HisPro: 1.175 ± 0.03
0.551HisGln: 0.551 ± 0.019
1.203HisArg: 1.203 ± 0.029
1.015HisSer: 1.015 ± 0.026
1.049HisThr: 1.049 ± 0.025
1.313HisVal: 1.313 ± 0.029
0.275HisTrp: 0.275 ± 0.014
0.781HisTyr: 0.781 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
5.82IleAla: 5.82 ± 0.074
0.451IleCys: 0.451 ± 0.018
3.433IleAsp: 3.433 ± 0.044
4.3IleGlu: 4.3 ± 0.057
1.911IlePhe: 1.911 ± 0.037
5.157IleGly: 5.157 ± 0.076
1.056IleHis: 1.056 ± 0.029
2.437IleIle: 2.437 ± 0.049
2.123IleLys: 2.123 ± 0.045
4.869IleLeu: 4.869 ± 0.066
1.186IleMet: 1.186 ± 0.027
1.715IleAsn: 1.715 ± 0.035
2.578IlePro: 2.578 ± 0.046
1.788IleGln: 1.788 ± 0.038
3.953IleArg: 3.953 ± 0.053
3.183IleSer: 3.183 ± 0.052
2.699IleThr: 2.699 ± 0.048
4.854IleVal: 4.854 ± 0.064
0.604IleTrp: 0.604 ± 0.021
1.542IleTyr: 1.542 ± 0.033
0.0IleXaa: 0.0 ± 0.0
Lys
3.671LysAla: 3.671 ± 0.061
0.164LysCys: 0.164 ± 0.01
2.077LysAsp: 2.077 ± 0.043
3.023LysGlu: 3.023 ± 0.055
1.323LysPhe: 1.323 ± 0.032
2.727LysGly: 2.727 ± 0.049
0.865LysHis: 0.865 ± 0.023
2.54LysIle: 2.54 ± 0.052
2.664LysLys: 2.664 ± 0.055
4.648LysLeu: 4.648 ± 0.063
1.302LysMet: 1.302 ± 0.03
1.609LysAsn: 1.609 ± 0.037
2.004LysPro: 2.004 ± 0.035
1.767LysGln: 1.767 ± 0.038
2.768LysArg: 2.768 ± 0.049
2.521LysSer: 2.521 ± 0.047
2.529LysThr: 2.529 ± 0.044
2.836LysVal: 2.836 ± 0.058
0.575LysTrp: 0.575 ± 0.021
1.302LysTyr: 1.302 ± 0.034
0.0LysXaa: 0.0 ± 0.0
Leu
9.413LeuAla: 9.413 ± 0.099
0.769LeuCys: 0.769 ± 0.025
5.676LeuAsp: 5.676 ± 0.065
6.714LeuGlu: 6.714 ± 0.089
4.142LeuPhe: 4.142 ± 0.072
7.658LeuGly: 7.658 ± 0.082
2.224LeuHis: 2.224 ± 0.04
5.896LeuIle: 5.896 ± 0.074
4.389LeuLys: 4.389 ± 0.065
11.631LeuLeu: 11.631 ± 0.159
2.407LeuMet: 2.407 ± 0.038
3.728LeuAsn: 3.728 ± 0.062
5.03LeuPro: 5.03 ± 0.06
3.482LeuGln: 3.482 ± 0.042
6.299LeuArg: 6.299 ± 0.081
6.85LeuSer: 6.85 ± 0.067
5.762LeuThr: 5.762 ± 0.067
5.992LeuVal: 5.992 ± 0.072
0.956LeuTrp: 0.956 ± 0.03
3.154LeuTyr: 3.154 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
2.045MetAla: 2.045 ± 0.038
0.137MetCys: 0.137 ± 0.008
1.324MetAsp: 1.324 ± 0.031
1.646MetGlu: 1.646 ± 0.034
0.837MetPhe: 0.837 ± 0.025
1.602MetGly: 1.602 ± 0.033
0.475MetHis: 0.475 ± 0.017
1.546MetIle: 1.546 ± 0.03
1.431MetLys: 1.431 ± 0.032
2.846MetLeu: 2.846 ± 0.042
0.692MetMet: 0.692 ± 0.024
1.352MetAsn: 1.352 ± 0.028
1.214MetPro: 1.214 ± 0.027
1.114MetGln: 1.114 ± 0.03
1.584MetArg: 1.584 ± 0.029
1.561MetSer: 1.561 ± 0.032
1.699MetThr: 1.699 ± 0.034
1.399MetVal: 1.399 ± 0.032
0.22MetTrp: 0.22 ± 0.012
0.623MetTyr: 0.623 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
3.053AsnAla: 3.053 ± 0.054
0.19AsnCys: 0.19 ± 0.012
1.615AsnAsp: 1.615 ± 0.039
2.226AsnGlu: 2.226 ± 0.035
1.203AsnPhe: 1.203 ± 0.03
2.964AsnGly: 2.964 ± 0.055
0.602AsnHis: 0.602 ± 0.02
1.694AsnIle: 1.694 ± 0.037
1.372AsnLys: 1.372 ± 0.036
3.052AsnLeu: 3.052 ± 0.044
0.844AsnMet: 0.844 ± 0.025
1.062AsnAsn: 1.062 ± 0.036
1.913AsnPro: 1.913 ± 0.041
1.011AsnGln: 1.011 ± 0.027
2.111AsnArg: 2.111 ± 0.037
1.542AsnSer: 1.542 ± 0.034
1.669AsnThr: 1.669 ± 0.038
2.444AsnVal: 2.444 ± 0.049
0.409AsnTrp: 0.409 ± 0.017
1.067AsnTyr: 1.067 ± 0.03
0.0AsnXaa: 0.0 ± 0.0
Pro
4.978ProAla: 4.978 ± 0.079
0.227ProCys: 0.227 ± 0.011
3.024ProAsp: 3.024 ± 0.044
3.928ProGlu: 3.928 ± 0.066
1.814ProPhe: 1.814 ± 0.035
3.74ProGly: 3.74 ± 0.05
0.873ProHis: 0.873 ± 0.026
2.288ProIle: 2.288 ± 0.039
1.628ProLys: 1.628 ± 0.032
4.182ProLeu: 4.182 ± 0.06
0.951ProMet: 0.951 ± 0.025
1.57ProAsn: 1.57 ± 0.037
1.6ProPro: 1.6 ± 0.055
1.584ProGln: 1.584 ± 0.034
1.75ProArg: 1.75 ± 0.036
2.595ProSer: 2.595 ± 0.043
2.008ProThr: 2.008 ± 0.06
3.344ProVal: 3.344 ± 0.052
0.479ProTrp: 0.479 ± 0.02
1.435ProTyr: 1.435 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
4.041GlnAla: 4.041 ± 0.058
0.141GlnCys: 0.141 ± 0.009
1.555GlnAsp: 1.555 ± 0.03
2.277GlnGlu: 2.277 ± 0.042
1.236GlnPhe: 1.236 ± 0.029
2.646GlnGly: 2.646 ± 0.04
0.674GlnHis: 0.674 ± 0.023
2.195GlnIle: 2.195 ± 0.042
1.562GlnLys: 1.562 ± 0.041
3.552GlnLeu: 3.552 ± 0.06
0.986GlnMet: 0.986 ± 0.025
1.142GlnAsn: 1.142 ± 0.029
1.658GlnPro: 1.658 ± 0.032
1.589GlnGln: 1.589 ± 0.041
2.2GlnArg: 2.2 ± 0.04
2.167GlnSer: 2.167 ± 0.039
2.077GlnThr: 2.077 ± 0.04
2.259GlnVal: 2.259 ± 0.038
0.423GlnTrp: 0.423 ± 0.019
1.065GlnTyr: 1.065 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
5.102ArgAla: 5.102 ± 0.074
0.382ArgCys: 0.382 ± 0.018
2.966ArgAsp: 2.966 ± 0.042
5.116ArgGlu: 5.116 ± 0.086
2.519ArgPhe: 2.519 ± 0.04
4.241ArgGly: 4.241 ± 0.053
1.317ArgHis: 1.317 ± 0.032
4.122ArgIle: 4.122 ± 0.052
2.766ArgLys: 2.766 ± 0.047
6.971ArgLeu: 6.971 ± 0.093
1.907ArgMet: 1.907 ± 0.038
1.67ArgAsn: 1.67 ± 0.032
2.609ArgPro: 2.609 ± 0.049
2.443ArgGln: 2.443 ± 0.047
4.463ArgArg: 4.463 ± 0.082
4.165ArgSer: 4.165 ± 0.062
3.435ArgThr: 3.435 ± 0.053
3.66ArgVal: 3.66 ± 0.058
0.717ArgTrp: 0.717 ± 0.024
2.166ArgTyr: 2.166 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
6.434SerAla: 6.434 ± 0.083
0.355SerCys: 0.355 ± 0.015
3.168SerAsp: 3.168 ± 0.052
4.016SerGlu: 4.016 ± 0.068
2.675SerPhe: 2.675 ± 0.044
6.469SerGly: 6.469 ± 0.079
1.02SerHis: 1.02 ± 0.023
3.448SerIle: 3.448 ± 0.053
2.397SerLys: 2.397 ± 0.045
6.108SerLeu: 6.108 ± 0.07
1.547SerMet: 1.547 ± 0.03
1.757SerAsn: 1.757 ± 0.041
2.546SerPro: 2.546 ± 0.041
1.798SerGln: 1.798 ± 0.04
3.645SerArg: 3.645 ± 0.056
3.948SerSer: 3.948 ± 0.064
2.874SerThr: 2.874 ± 0.055
4.582SerVal: 4.582 ± 0.058
0.71SerTrp: 0.71 ± 0.023
1.916SerTyr: 1.916 ± 0.034
0.0SerXaa: 0.0 ± 0.0
Thr
5.208ThrAla: 5.208 ± 0.071
0.285ThrCys: 0.285 ± 0.014
3.052ThrAsp: 3.052 ± 0.054
3.35ThrGlu: 3.35 ± 0.061
2.289ThrPhe: 2.289 ± 0.046
4.772ThrGly: 4.772 ± 0.063
0.954ThrHis: 0.954 ± 0.024
2.984ThrIle: 2.984 ± 0.052
1.837ThrLys: 1.837 ± 0.039
5.792ThrLeu: 5.792 ± 0.064
1.107ThrMet: 1.107 ± 0.023
1.604ThrAsn: 1.604 ± 0.039
2.868ThrPro: 2.868 ± 0.072
1.665ThrGln: 1.665 ± 0.039
2.577ThrArg: 2.577 ± 0.039
2.961ThrSer: 2.961 ± 0.05
2.736ThrThr: 2.736 ± 0.074
4.406ThrVal: 4.406 ± 0.091
0.528ThrTrp: 0.528 ± 0.019
1.81ThrTyr: 1.81 ± 0.04
0.0ThrXaa: 0.0 ± 0.0
Val
5.187ValAla: 5.187 ± 0.067
0.604ValCys: 0.604 ± 0.021
3.642ValAsp: 3.642 ± 0.052
4.406ValGlu: 4.406 ± 0.054
2.817ValPhe: 2.817 ± 0.045
4.806ValGly: 4.806 ± 0.062
1.418ValHis: 1.418 ± 0.027
3.93ValIle: 3.93 ± 0.061
3.022ValLys: 3.022 ± 0.049
7.389ValLeu: 7.389 ± 0.088
1.729ValMet: 1.729 ± 0.039
2.487ValAsn: 2.487 ± 0.047
3.36ValPro: 3.36 ± 0.052
2.505ValGln: 2.505 ± 0.038
4.544ValArg: 4.544 ± 0.067
4.768ValSer: 4.768 ± 0.068
4.317ValThr: 4.317 ± 0.082
4.908ValVal: 4.908 ± 0.067
0.819ValTrp: 0.819 ± 0.026
2.339ValTyr: 2.339 ± 0.048
0.0ValXaa: 0.0 ± 0.0
Trp
0.941TrpAla: 0.941 ± 0.026
0.071TrpCys: 0.071 ± 0.007
0.624TrpAsp: 0.624 ± 0.022
0.703TrpGlu: 0.703 ± 0.02
0.465TrpPhe: 0.465 ± 0.02
0.764TrpGly: 0.764 ± 0.027
0.245TrpHis: 0.245 ± 0.014
0.886TrpIle: 0.886 ± 0.025
0.597TrpLys: 0.597 ± 0.021
1.227TrpLeu: 1.227 ± 0.03
0.414TrpMet: 0.414 ± 0.016
0.67TrpAsn: 0.67 ± 0.024
0.382TrpPro: 0.382 ± 0.016
0.457TrpGln: 0.457 ± 0.018
0.599TrpArg: 0.599 ± 0.02
0.735TrpSer: 0.735 ± 0.025
0.817TrpThr: 0.817 ± 0.024
0.695TrpVal: 0.695 ± 0.022
0.159TrpTrp: 0.159 ± 0.01
0.333TrpTyr: 0.333 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.361TyrAla: 3.361 ± 0.055
0.229TyrCys: 0.229 ± 0.012
1.92TyrAsp: 1.92 ± 0.047
2.247TyrGlu: 2.247 ± 0.042
1.355TyrPhe: 1.355 ± 0.031
2.855TyrGly: 2.855 ± 0.044
0.578TyrHis: 0.578 ± 0.021
1.509TyrIle: 1.509 ± 0.034
1.293TyrLys: 1.293 ± 0.033
3.079TyrLeu: 3.079 ± 0.055
0.834TyrMet: 0.834 ± 0.023
1.062TyrAsn: 1.062 ± 0.031
1.468TyrPro: 1.468 ± 0.032
0.881TyrGln: 0.881 ± 0.023
2.343TyrArg: 2.343 ± 0.039
1.701TyrSer: 1.701 ± 0.039
1.773TyrThr: 1.773 ± 0.038
2.353TyrVal: 2.353 ± 0.043
0.401TyrTrp: 0.401 ± 0.015
1.119TyrTyr: 1.119 ± 0.033
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4543 proteins (1557127 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski