Pseudodesulfovibrio sp. JC047

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfovibrionaceae; Pseudodesulfovibrio; unclassified Pseudodesulfovibrio

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3293 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L9LWR8|A0A6L9LWR8_9DELT ROK family protein OS=Pseudodesulfovibrio sp. JC047 OX=2683199 GN=GO013_04535 PE=3 SV=1
MM1 pKa = 7.99PFNQDD6 pKa = 2.93NMSLQSGYY14 pKa = 9.59PGKK17 pKa = 10.85QMFTYY22 pKa = 10.29HH23 pKa = 6.41SADD26 pKa = 3.53TIEE29 pKa = 4.25TVAEE33 pKa = 3.59NGYY36 pKa = 10.72FNDD39 pKa = 5.48AISNNNLTTGDD50 pKa = 3.83IITVVDD56 pKa = 3.66NGTPTVDD63 pKa = 3.26VVVVTVNGSMATVTNGTT80 pKa = 3.66

Molecular weight: 8.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L9LWB8|A0A6L9LWB8_9DELT Na(+)/H(+) antiporter NhaA OS=Pseudodesulfovibrio sp. JC047 OX=2683199 GN=nhaA PE=3 SV=1
MM1 pKa = 7.7PKK3 pKa = 9.95IKK5 pKa = 9.47TRR7 pKa = 11.84RR8 pKa = 11.84AAAKK12 pKa = 9.74RR13 pKa = 11.84FSKK16 pKa = 9.7TATGKK21 pKa = 10.1FKK23 pKa = 10.74RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84KK27 pKa = 9.07NLRR30 pKa = 11.84HH31 pKa = 6.24ILTKK35 pKa = 10.67KK36 pKa = 7.68NAKK39 pKa = 9.22RR40 pKa = 11.84KK41 pKa = 9.18RR42 pKa = 11.84RR43 pKa = 11.84LGQSTTVDD51 pKa = 3.53TANMKK56 pKa = 10.08AVRR59 pKa = 11.84RR60 pKa = 11.84QLPNGG65 pKa = 3.51

Molecular weight: 7.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3293

0

3293

1039952

29

3977

315.8

34.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.947 ± 0.045

1.364 ± 0.021

5.823 ± 0.047

6.337 ± 0.039

4.217 ± 0.031

7.776 ± 0.036

2.207 ± 0.024

5.983 ± 0.036

5.214 ± 0.047

9.896 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.986 ± 0.024

3.337 ± 0.025

4.522 ± 0.027

3.321 ± 0.024

5.327 ± 0.035

5.698 ± 0.027

5.798 ± 0.038

7.32 ± 0.041

1.209 ± 0.018

2.716 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski