Lactobacillus saniviri JCM 17471 = DSM 24301
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2333 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R2MRC9|A0A0R2MRC9_9LACO Regulatory protein RecX OS=Lactobacillus saniviri JCM 17471 = DSM 24301 OX=1293598 GN=recX PE=3 SV=1
MM1 pKa = 6.73 MTLLTQPKK9 pKa = 9.34 RR10 pKa = 11.84 VFAGQVYY17 pKa = 9.22 MIEE20 pKa = 4.27 NSDD23 pKa = 3.69 EE24 pKa = 3.97 QGSFYY29 pKa = 10.53 QAWQEE34 pKa = 4.07 FTDD37 pKa = 4.52 AGYY40 pKa = 10.8 FDD42 pKa = 4.63 QLDD45 pKa = 3.95 ALAEE49 pKa = 4.43 TPNRR53 pKa = 11.84 TAMLVFSPYY62 pKa = 9.59 GTFQYY67 pKa = 10.23 WIGSVLPEE75 pKa = 5.05 DD76 pKa = 4.1 ADD78 pKa = 3.83 APEE81 pKa = 4.29 GLQRR85 pKa = 11.84 LILPEE90 pKa = 4.12 GTAGIVTQPTNGFAPQIPVEE110 pKa = 4.02 MAFNKK115 pKa = 10.02 GLEE118 pKa = 4.4 VIEE121 pKa = 4.38 KK122 pKa = 10.44 AGFPLPSHH130 pKa = 7.34 IGQTDD135 pKa = 3.01 NPYY138 pKa = 10.54 FLEE141 pKa = 4.87 SYY143 pKa = 10.6 LLTDD147 pKa = 4.3 GEE149 pKa = 4.43 ISDD152 pKa = 3.91 VRR154 pKa = 11.84 YY155 pKa = 7.97 TLYY158 pKa = 10.52 INPDD162 pKa = 2.89 QMDD165 pKa = 4.35 GYY167 pKa = 11.38 DD168 pKa = 3.39 EE169 pKa = 5.21 FEE171 pKa = 3.9
Molecular weight: 19.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.834
IPC_protein 3.783
Toseland 3.592
ProMoST 3.91
Dawson 3.757
Bjellqvist 3.961
Wikipedia 3.668
Rodwell 3.617
Grimsley 3.503
Solomon 3.745
Lehninger 3.706
Nozaki 3.884
DTASelect 4.05
Thurlkill 3.643
EMBOSS 3.681
Sillero 3.897
Patrickios 0.655
IPC_peptide 3.745
IPC2_peptide 3.884
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|A0A0R2MUH2|A0A0R2MUH2_9LACO Galactokinase OS=Lactobacillus saniviri JCM 17471 = DSM 24301 OX=1293598 GN=galK PE=3 SV=1
MM1 pKa = 5.72 TTKK4 pKa = 9.85 RR5 pKa = 11.84 TFQPKK10 pKa = 8.33 KK11 pKa = 7.5 RR12 pKa = 11.84 HH13 pKa = 5.56 RR14 pKa = 11.84 EE15 pKa = 3.79 RR16 pKa = 11.84 VHH18 pKa = 7.7 GFMKK22 pKa = 10.56 RR23 pKa = 11.84 MSTKK27 pKa = 10.26 NGRR30 pKa = 11.84 KK31 pKa = 8.58 VLARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 AKK39 pKa = 9.94 GRR41 pKa = 11.84 KK42 pKa = 8.67 VLSAA46 pKa = 4.05
Molecular weight: 5.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.406
IPC2_protein 10.877
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2333
0
2333
699316
46
1791
299.7
33.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.437 ± 0.065
0.243 ± 0.009
6.047 ± 0.05
4.991 ± 0.06
4.068 ± 0.038
6.887 ± 0.055
2.144 ± 0.03
6.567 ± 0.045
5.192 ± 0.058
9.886 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.802 ± 0.026
4.324 ± 0.043
4.018 ± 0.03
5.12 ± 0.051
4.145 ± 0.043
5.58 ± 0.051
6.733 ± 0.05
7.327 ± 0.043
1.141 ± 0.023
3.348 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here