Sulfolobus sp. SCGC AB-777_G06
Average proteome isoelectric point is 7.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1085 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T9X4N7|A0A2T9X4N7_9CREN Uncharacterized protein OS=Sulfolobus sp. SCGC AB-777_G06 OX=1987491 GN=DDW11_04245 PE=4 SV=1
MM1 pKa = 7.34 LRR3 pKa = 11.84 DD4 pKa = 3.8 FEE6 pKa = 5.45 EE7 pKa = 4.45 IEE9 pKa = 4.43 CTKK12 pKa = 10.9 EE13 pKa = 3.65 EE14 pKa = 4.63 YY15 pKa = 10.89 YY16 pKa = 11.19 DD17 pKa = 4.09 DD18 pKa = 4.13 LGKK21 pKa = 10.41 FHH23 pKa = 7.08 EE24 pKa = 4.63 VPYY27 pKa = 10.0 YY28 pKa = 11.01 VPAKK32 pKa = 10.14 CYY34 pKa = 8.68 MKK36 pKa = 10.21 EE37 pKa = 4.19 YY38 pKa = 10.04 EE39 pKa = 4.5 WGTATILEE47 pKa = 4.84 DD48 pKa = 5.38 DD49 pKa = 4.92 LDD51 pKa = 5.58 LGDD54 pKa = 5.25 SIIVYY59 pKa = 10.17
Molecular weight: 7.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.904
IPC2_protein 4.05
IPC_protein 3.948
Toseland 3.757
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.075
Wikipedia 3.821
Rodwell 3.783
Grimsley 3.681
Solomon 3.897
Lehninger 3.846
Nozaki 4.037
DTASelect 4.19
Thurlkill 3.808
EMBOSS 3.834
Sillero 4.062
Patrickios 0.362
IPC_peptide 3.897
IPC2_peptide 4.037
IPC2.peptide.svr19 3.956
Protein with the highest isoelectric point:
>tr|A0A2T9X4U4|A0A2T9X4U4_9CREN 50S ribosomal protein L18 OS=Sulfolobus sp. SCGC AB-777_G06 OX=1987491 GN=rpl18 PE=3 SV=1
MM1 pKa = 8.14 PSPQLRR7 pKa = 11.84 SRR9 pKa = 11.84 SLRR12 pKa = 11.84 RR13 pKa = 11.84 VQVRR17 pKa = 11.84 TPSGRR22 pKa = 11.84 TVTHH26 pKa = 6.41 YY27 pKa = 10.7 EE28 pKa = 4.03 DD29 pKa = 4.26 KK30 pKa = 11.02 KK31 pKa = 11.07 NSQALCAICKK41 pKa = 10.03 KK42 pKa = 9.84 PLRR45 pKa = 11.84 GVKK48 pKa = 8.36 TNKK51 pKa = 7.52 IHH53 pKa = 7.62 KK54 pKa = 10.03 YY55 pKa = 7.76 STTEE59 pKa = 3.65 KK60 pKa = 10.29 RR61 pKa = 11.84 PEE63 pKa = 4.01 RR64 pKa = 11.84 PFGGYY69 pKa = 9.47 LCHH72 pKa = 7.56 DD73 pKa = 3.88 CLEE76 pKa = 4.31 RR77 pKa = 11.84 LIKK80 pKa = 10.49 LAVRR84 pKa = 11.84 GFF86 pKa = 3.48
Molecular weight: 9.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.677
IPC_protein 10.16
Toseland 10.687
ProMoST 10.277
Dawson 10.774
Bjellqvist 10.452
Wikipedia 10.935
Rodwell 11.155
Grimsley 10.818
Solomon 10.862
Lehninger 10.833
Nozaki 10.687
DTASelect 10.438
Thurlkill 10.687
EMBOSS 11.082
Sillero 10.716
Patrickios 10.906
IPC_peptide 10.862
IPC2_peptide 9.531
IPC2.peptide.svr19 8.537
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1085
0
1085
276833
19
1575
255.1
28.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.725 ± 0.081
0.64 ± 0.03
4.751 ± 0.069
7.078 ± 0.104
4.347 ± 0.065
6.596 ± 0.062
1.307 ± 0.028
8.869 ± 0.07
7.808 ± 0.118
10.276 ± 0.094
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.193 ± 0.033
4.519 ± 0.07
3.932 ± 0.06
2.259 ± 0.044
4.482 ± 0.074
6.561 ± 0.077
4.941 ± 0.066
7.973 ± 0.068
0.97 ± 0.027
4.772 ± 0.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here