Amino acid dipepetide frequency for alpha proteobacterium AAP81b

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
28.251AlaAla: 28.251 ± 0.348
1.046AlaCys: 1.046 ± 0.038
8.859AlaAsp: 8.859 ± 0.107
8.244AlaGlu: 8.244 ± 0.117
4.695AlaPhe: 4.695 ± 0.072
15.43AlaGly: 15.43 ± 0.218
2.392AlaHis: 2.392 ± 0.061
7.083AlaIle: 7.083 ± 0.087
3.552AlaLys: 3.552 ± 0.085
16.461AlaLeu: 16.461 ± 0.184
3.995AlaMet: 3.995 ± 0.079
3.158AlaAsn: 3.158 ± 0.099
8.057AlaPro: 8.057 ± 0.13
3.925AlaGln: 3.925 ± 0.075
12.459AlaArg: 12.459 ± 0.18
6.749AlaSer: 6.749 ± 0.117
8.326AlaThr: 8.326 ± 0.136
10.311AlaVal: 10.311 ± 0.117
2.088AlaTrp: 2.088 ± 0.056
2.375AlaTyr: 2.375 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.889CysAla: 0.889 ± 0.035
0.061CysCys: 0.061 ± 0.008
0.493CysAsp: 0.493 ± 0.027
0.325CysGlu: 0.325 ± 0.019
0.238CysPhe: 0.238 ± 0.014
0.81CysGly: 0.81 ± 0.033
0.223CysHis: 0.223 ± 0.021
0.306CysIle: 0.306 ± 0.02
0.118CysLys: 0.118 ± 0.013
0.689CysLeu: 0.689 ± 0.03
0.107CysMet: 0.107 ± 0.012
0.189CysAsn: 0.189 ± 0.015
0.424CysPro: 0.424 ± 0.025
0.166CysGln: 0.166 ± 0.013
0.567CysArg: 0.567 ± 0.028
0.341CysSer: 0.341 ± 0.019
0.34CysThr: 0.34 ± 0.022
0.46CysVal: 0.46 ± 0.023
0.082CysTrp: 0.082 ± 0.01
0.134CysTyr: 0.134 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
8.828AspAla: 8.828 ± 0.119
0.452AspCys: 0.452 ± 0.027
3.575AspAsp: 3.575 ± 0.077
2.643AspGlu: 2.643 ± 0.07
2.401AspPhe: 2.401 ± 0.059
5.898AspGly: 5.898 ± 0.099
1.1AspHis: 1.1 ± 0.04
2.864AspIle: 2.864 ± 0.071
1.381AspLys: 1.381 ± 0.046
5.981AspLeu: 5.981 ± 0.084
1.13AspMet: 1.13 ± 0.043
1.359AspAsn: 1.359 ± 0.046
3.959AspPro: 3.959 ± 0.072
1.407AspGln: 1.407 ± 0.042
4.776AspArg: 4.776 ± 0.076
2.248AspSer: 2.248 ± 0.057
2.668AspThr: 2.668 ± 0.071
3.944AspVal: 3.944 ± 0.064
1.238AspTrp: 1.238 ± 0.035
1.55AspTyr: 1.55 ± 0.049
0.0AspXaa: 0.0 ± 0.0
Glu
8.053GluAla: 8.053 ± 0.126
0.24GluCys: 0.24 ± 0.019
1.98GluAsp: 1.98 ± 0.048
1.874GluGlu: 1.874 ± 0.059
1.387GluPhe: 1.387 ± 0.039
3.616GluGly: 3.616 ± 0.068
0.839GluHis: 0.839 ± 0.035
2.784GluIle: 2.784 ± 0.061
1.33GluLys: 1.33 ± 0.049
4.613GluLeu: 4.613 ± 0.08
1.109GluMet: 1.109 ± 0.04
1.009GluAsn: 1.009 ± 0.033
2.161GluPro: 2.161 ± 0.052
1.344GluGln: 1.344 ± 0.039
4.039GluArg: 4.039 ± 0.086
1.778GluSer: 1.778 ± 0.045
2.954GluThr: 2.954 ± 0.06
3.671GluVal: 3.671 ± 0.079
0.643GluTrp: 0.643 ± 0.027
0.734GluTyr: 0.734 ± 0.033
0.0GluXaa: 0.0 ± 0.0
Phe
5.39PheAla: 5.39 ± 0.077
0.275PheCys: 0.275 ± 0.02
2.795PheAsp: 2.795 ± 0.052
1.828PheGlu: 1.828 ± 0.044
1.188PhePhe: 1.188 ± 0.038
3.963PheGly: 3.963 ± 0.072
0.656PheHis: 0.656 ± 0.031
1.339PheIle: 1.339 ± 0.037
0.744PheLys: 0.744 ± 0.036
2.934PheLeu: 2.934 ± 0.067
0.537PheMet: 0.537 ± 0.024
0.923PheAsn: 0.923 ± 0.042
1.517PhePro: 1.517 ± 0.039
0.849PheGln: 0.849 ± 0.03
2.212PheArg: 2.212 ± 0.052
1.62PheSer: 1.62 ± 0.041
2.128PheThr: 2.128 ± 0.058
2.772PheVal: 2.772 ± 0.058
0.443PheTrp: 0.443 ± 0.023
0.843PheTyr: 0.843 ± 0.03
0.0PheXaa: 0.0 ± 0.0
Gly
11.985GlyAla: 11.985 ± 0.169
0.753GlyCys: 0.753 ± 0.028
5.753GlyAsp: 5.753 ± 0.107
4.64GlyGlu: 4.64 ± 0.077
3.975GlyPhe: 3.975 ± 0.079
9.565GlyGly: 9.565 ± 0.224
1.829GlyHis: 1.829 ± 0.054
4.42GlyIle: 4.42 ± 0.07
2.775GlyLys: 2.775 ± 0.064
9.787GlyLeu: 9.787 ± 0.103
2.128GlyMet: 2.128 ± 0.054
2.8GlyAsn: 2.8 ± 0.14
3.899GlyPro: 3.899 ± 0.064
2.832GlyGln: 2.832 ± 0.064
7.013GlyArg: 7.013 ± 0.094
5.002GlySer: 5.002 ± 0.146
5.051GlyThr: 5.051 ± 0.282
6.913GlyVal: 6.913 ± 0.108
1.646GlyTrp: 1.646 ± 0.054
2.16GlyTyr: 2.16 ± 0.049
0.0GlyXaa: 0.0 ± 0.0
His
2.39HisAla: 2.39 ± 0.062
0.222HisCys: 0.222 ± 0.017
1.221HisAsp: 1.221 ± 0.04
0.778HisGlu: 0.778 ± 0.032
0.749HisPhe: 0.749 ± 0.028
1.89HisGly: 1.89 ± 0.052
0.461HisHis: 0.461 ± 0.027
0.835HisIle: 0.835 ± 0.029
0.376HisLys: 0.376 ± 0.02
1.762HisLeu: 1.762 ± 0.048
0.392HisMet: 0.392 ± 0.021
0.43HisAsn: 0.43 ± 0.025
1.217HisPro: 1.217 ± 0.04
0.447HisGln: 0.447 ± 0.024
1.386HisArg: 1.386 ± 0.047
0.757HisSer: 0.757 ± 0.035
0.588HisThr: 0.588 ± 0.023
1.403HisVal: 1.403 ± 0.039
0.347HisTrp: 0.347 ± 0.022
0.485HisTyr: 0.485 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
8.422IleAla: 8.422 ± 0.114
0.302IleCys: 0.302 ± 0.018
3.971IleAsp: 3.971 ± 0.07
2.785IleGlu: 2.785 ± 0.059
1.357IlePhe: 1.357 ± 0.047
4.963IleGly: 4.963 ± 0.07
0.76IleHis: 0.76 ± 0.03
1.83IleIle: 1.83 ± 0.049
1.066IleLys: 1.066 ± 0.04
3.443IleLeu: 3.443 ± 0.053
0.699IleMet: 0.699 ± 0.03
1.208IleAsn: 1.208 ± 0.047
1.93IlePro: 1.93 ± 0.049
0.834IleGln: 0.834 ± 0.031
2.725IleArg: 2.725 ± 0.057
1.991IleSer: 1.991 ± 0.063
2.61IleThr: 2.61 ± 0.088
4.274IleVal: 4.274 ± 0.067
0.519IleTrp: 0.519 ± 0.024
0.816IleTyr: 0.816 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
3.787LysAla: 3.787 ± 0.083
0.12LysCys: 0.12 ± 0.012
1.214LysAsp: 1.214 ± 0.047
0.835LysGlu: 0.835 ± 0.037
0.73LysPhe: 0.73 ± 0.028
2.034LysGly: 2.034 ± 0.06
0.422LysHis: 0.422 ± 0.026
1.068LysIle: 1.068 ± 0.04
0.727LysLys: 0.727 ± 0.042
2.735LysLeu: 2.735 ± 0.062
0.561LysMet: 0.561 ± 0.028
0.508LysAsn: 0.508 ± 0.024
1.617LysPro: 1.617 ± 0.053
0.59LysGln: 0.59 ± 0.025
1.778LysArg: 1.778 ± 0.056
1.158LysSer: 1.158 ± 0.044
1.425LysThr: 1.425 ± 0.042
1.855LysVal: 1.855 ± 0.051
0.293LysTrp: 0.293 ± 0.016
0.451LysTyr: 0.451 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
18.865LeuAla: 18.865 ± 0.218
0.685LeuCys: 0.685 ± 0.03
6.109LeuAsp: 6.109 ± 0.083
3.925LeuGlu: 3.925 ± 0.074
3.32LeuPhe: 3.32 ± 0.075
9.189LeuGly: 9.189 ± 0.125
1.737LeuHis: 1.737 ± 0.05
4.476LeuIle: 4.476 ± 0.07
2.832LeuLys: 2.832 ± 0.077
10.102LeuLeu: 10.102 ± 0.157
1.848LeuMet: 1.848 ± 0.048
2.1LeuAsn: 2.1 ± 0.082
6.276LeuPro: 6.276 ± 0.086
2.35LeuGln: 2.35 ± 0.056
7.144LeuArg: 7.144 ± 0.104
4.747LeuSer: 4.747 ± 0.078
6.1LeuThr: 6.1 ± 0.143
8.21LeuVal: 8.21 ± 0.103
1.255LeuTrp: 1.255 ± 0.041
1.828LeuTyr: 1.828 ± 0.044
0.0LeuXaa: 0.0 ± 0.0
Met
3.384MetAla: 3.384 ± 0.077
0.127MetCys: 0.127 ± 0.013
0.848MetAsp: 0.848 ± 0.035
0.714MetGlu: 0.714 ± 0.028
0.574MetPhe: 0.574 ± 0.028
1.553MetGly: 1.553 ± 0.048
0.382MetHis: 0.382 ± 0.022
1.06MetIle: 1.06 ± 0.039
0.652MetLys: 0.652 ± 0.028
2.216MetLeu: 2.216 ± 0.051
0.465MetMet: 0.465 ± 0.023
0.532MetAsn: 0.532 ± 0.026
1.297MetPro: 1.297 ± 0.041
0.567MetGln: 0.567 ± 0.023
1.51MetArg: 1.51 ± 0.045
1.091MetSer: 1.091 ± 0.038
1.657MetThr: 1.657 ± 0.043
1.488MetVal: 1.488 ± 0.043
0.183MetTrp: 0.183 ± 0.015
0.231MetTyr: 0.231 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.197AsnAla: 3.197 ± 0.096
0.219AsnCys: 0.219 ± 0.016
1.438AsnAsp: 1.438 ± 0.073
0.817AsnGlu: 0.817 ± 0.028
0.978AsnPhe: 0.978 ± 0.047
2.278AsnGly: 2.278 ± 0.13
0.417AsnHis: 0.417 ± 0.026
1.313AsnIle: 1.313 ± 0.049
0.501AsnLys: 0.501 ± 0.025
2.383AsnLeu: 2.383 ± 0.069
0.417AsnMet: 0.417 ± 0.022
0.741AsnAsn: 0.741 ± 0.053
1.657AsnPro: 1.657 ± 0.046
0.592AsnGln: 0.592 ± 0.027
1.678AsnArg: 1.678 ± 0.049
1.179AsnSer: 1.179 ± 0.061
1.062AsnThr: 1.062 ± 0.051
1.766AsnVal: 1.766 ± 0.058
0.35AsnTrp: 0.35 ± 0.021
0.57AsnTyr: 0.57 ± 0.029
0.0AsnXaa: 0.0 ± 0.0
Pro
9.2ProAla: 9.2 ± 0.149
0.334ProCys: 0.334 ± 0.02
3.614ProAsp: 3.614 ± 0.068
3.241ProGlu: 3.241 ± 0.071
1.806ProPhe: 1.806 ± 0.051
5.934ProGly: 5.934 ± 0.09
0.99ProHis: 0.99 ± 0.034
2.128ProIle: 2.128 ± 0.052
1.3ProLys: 1.3 ± 0.043
5.326ProLeu: 5.326 ± 0.083
1.092ProMet: 1.092 ± 0.031
1.089ProAsn: 1.089 ± 0.038
3.797ProPro: 3.797 ± 0.092
1.567ProGln: 1.567 ± 0.043
3.705ProArg: 3.705 ± 0.072
2.179ProSer: 2.179 ± 0.054
2.567ProThr: 2.567 ± 0.056
4.267ProVal: 4.267 ± 0.07
0.778ProTrp: 0.778 ± 0.033
0.955ProTyr: 0.955 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
3.977GlnAla: 3.977 ± 0.066
0.195GlnCys: 0.195 ± 0.015
1.062GlnAsp: 1.062 ± 0.039
0.894GlnGlu: 0.894 ± 0.033
0.957GlnPhe: 0.957 ± 0.036
2.357GlnGly: 2.357 ± 0.059
0.433GlnHis: 0.433 ± 0.022
1.304GlnIle: 1.304 ± 0.041
0.619GlnLys: 0.619 ± 0.031
3.046GlnLeu: 3.046 ± 0.072
0.557GlnMet: 0.557 ± 0.025
0.62GlnAsn: 0.62 ± 0.03
1.546GlnPro: 1.546 ± 0.041
0.837GlnGln: 0.837 ± 0.037
2.094GlnArg: 2.094 ± 0.051
1.297GlnSer: 1.297 ± 0.038
1.316GlnThr: 1.316 ± 0.043
2.164GlnVal: 2.164 ± 0.05
0.386GlnTrp: 0.386 ± 0.017
0.487GlnTyr: 0.487 ± 0.021
0.0GlnXaa: 0.0 ± 0.0
Arg
10.332ArgAla: 10.332 ± 0.133
0.513ArgCys: 0.513 ± 0.022
4.702ArgAsp: 4.702 ± 0.095
3.313ArgGlu: 3.313 ± 0.073
3.094ArgPhe: 3.094 ± 0.067
5.736ArgGly: 5.736 ± 0.09
1.709ArgHis: 1.709 ± 0.054
3.782ArgIle: 3.782 ± 0.072
1.501ArgLys: 1.501 ± 0.047
9.648ArgLeu: 9.648 ± 0.12
1.589ArgMet: 1.589 ± 0.052
1.579ArgAsn: 1.579 ± 0.046
4.08ArgPro: 4.08 ± 0.086
2.283ArgGln: 2.283 ± 0.051
6.388ArgArg: 6.388 ± 0.116
3.16ArgSer: 3.16 ± 0.065
3.236ArgThr: 3.236 ± 0.068
5.316ArgVal: 5.316 ± 0.084
1.282ArgTrp: 1.282 ± 0.043
1.731ArgTyr: 1.731 ± 0.048
0.0ArgXaa: 0.0 ± 0.0
Ser
6.007SerAla: 6.007 ± 0.101
0.323SerCys: 0.323 ± 0.018
2.798SerAsp: 2.798 ± 0.053
1.908SerGlu: 1.908 ± 0.048
2.041SerPhe: 2.041 ± 0.053
5.078SerGly: 5.078 ± 0.14
0.812SerHis: 0.812 ± 0.034
2.131SerIle: 2.131 ± 0.064
0.978SerLys: 0.978 ± 0.033
4.888SerLeu: 4.888 ± 0.083
0.827SerMet: 0.827 ± 0.036
1.203SerAsn: 1.203 ± 0.051
2.697SerPro: 2.697 ± 0.062
1.194SerGln: 1.194 ± 0.036
3.044SerArg: 3.044 ± 0.066
2.011SerSer: 2.011 ± 0.058
1.994SerThr: 1.994 ± 0.061
3.38SerVal: 3.38 ± 0.064
0.661SerTrp: 0.661 ± 0.03
1.112SerTyr: 1.112 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
8.022ThrAla: 8.022 ± 0.172
0.349ThrCys: 0.349 ± 0.019
2.788ThrAsp: 2.788 ± 0.055
2.055ThrGlu: 2.055 ± 0.052
1.642ThrPhe: 1.642 ± 0.059
5.732ThrGly: 5.732 ± 0.177
0.872ThrHis: 0.872 ± 0.029
2.922ThrIle: 2.922 ± 0.081
0.949ThrLys: 0.949 ± 0.037
6.269ThrLeu: 6.269 ± 0.192
1.002ThrMet: 1.002 ± 0.03
1.233ThrAsn: 1.233 ± 0.06
3.817ThrPro: 3.817 ± 0.072
1.284ThrGln: 1.284 ± 0.037
3.802ThrArg: 3.802 ± 0.065
2.502ThrSer: 2.502 ± 0.078
3.026ThrThr: 3.026 ± 0.116
4.128ThrVal: 4.128 ± 0.106
0.615ThrTrp: 0.615 ± 0.025
0.966ThrTyr: 0.966 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
12.306ValAla: 12.306 ± 0.131
0.479ValCys: 0.479 ± 0.025
4.149ValAsp: 4.149 ± 0.077
3.948ValGlu: 3.948 ± 0.08
2.481ValPhe: 2.481 ± 0.056
5.893ValGly: 5.893 ± 0.092
1.371ValHis: 1.371 ± 0.044
3.64ValIle: 3.64 ± 0.06
1.675ValLys: 1.675 ± 0.053
6.911ValLeu: 6.911 ± 0.099
1.463ValMet: 1.463 ± 0.043
1.923ValAsn: 1.923 ± 0.068
4.149ValPro: 4.149 ± 0.069
1.763ValGln: 1.763 ± 0.046
5.452ValArg: 5.452 ± 0.08
3.577ValSer: 3.577 ± 0.069
5.156ValThr: 5.156 ± 0.102
5.983ValVal: 5.983 ± 0.09
0.915ValTrp: 0.915 ± 0.037
1.296ValTyr: 1.296 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.616TrpAla: 1.616 ± 0.046
0.116TrpCys: 0.116 ± 0.01
0.676TrpAsp: 0.676 ± 0.028
0.497TrpGlu: 0.497 ± 0.027
0.527TrpPhe: 0.527 ± 0.027
1.034TrpGly: 1.034 ± 0.037
0.341TrpHis: 0.341 ± 0.02
0.516TrpIle: 0.516 ± 0.024
0.349TrpLys: 0.349 ± 0.019
1.963TrpLeu: 1.963 ± 0.056
0.286TrpMet: 0.286 ± 0.017
0.397TrpAsn: 0.397 ± 0.022
0.792TrpPro: 0.792 ± 0.034
0.722TrpGln: 0.722 ± 0.028
1.476TrpArg: 1.476 ± 0.045
0.764TrpSer: 0.764 ± 0.03
0.866TrpThr: 0.866 ± 0.032
0.817TrpVal: 0.817 ± 0.03
0.27TrpTrp: 0.27 ± 0.02
0.293TrpTyr: 0.293 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.557TyrAla: 2.557 ± 0.056
0.154TyrCys: 0.154 ± 0.012
1.357TyrAsp: 1.357 ± 0.046
0.897TyrGlu: 0.897 ± 0.032
0.817TyrPhe: 0.817 ± 0.03
1.976TyrGly: 1.976 ± 0.045
0.386TyrHis: 0.386 ± 0.024
0.703TyrIle: 0.703 ± 0.029
0.481TyrLys: 0.481 ± 0.025
1.945TyrLeu: 1.945 ± 0.052
0.324TyrMet: 0.324 ± 0.021
0.552TyrAsn: 0.552 ± 0.026
0.98TyrPro: 0.98 ± 0.033
0.592TyrGln: 0.592 ± 0.028
1.723TyrArg: 1.723 ± 0.044
0.938TyrSer: 0.938 ± 0.031
0.908TyrThr: 0.908 ± 0.038
1.407TyrVal: 1.407 ± 0.039
0.32TyrTrp: 0.32 ± 0.02
0.486TyrTyr: 0.486 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3055 proteins (934311 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski