Cellulomonas sp. Leaf395

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Cellulomonadaceae; Cellulomonas; unclassified Cellulomonas

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3738 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0T0MKR7|A0A0T0MKR7_9CELL Uncharacterized protein OS=Cellulomonas sp. Leaf395 OX=1736362 GN=ASG23_06805 PE=4 SV=1
MM1 pKa = 7.49FPAQQSRR8 pKa = 11.84RR9 pKa = 11.84ASTVLATAGLAFASVLVPAAAATAAPAATACDD41 pKa = 3.28SRR43 pKa = 11.84TNNTYY48 pKa = 11.18DD49 pKa = 3.39KK50 pKa = 11.24VLGCVTVDD58 pKa = 3.03GVLEE62 pKa = 4.15HH63 pKa = 7.21EE64 pKa = 4.73EE65 pKa = 4.0AFQAIADD72 pKa = 3.91ANGGNRR78 pKa = 11.84AAGTTGYY85 pKa = 7.12TASVDD90 pKa = 3.87YY91 pKa = 11.12VVEE94 pKa = 4.12TLEE97 pKa = 4.04AAGWSVEE104 pKa = 3.73LDD106 pKa = 3.29EE107 pKa = 6.17FPFTFVPPPLLEE119 pKa = 3.98QLMPTQATYY128 pKa = 9.38STGVFTGTGYY138 pKa = 11.54GEE140 pKa = 4.09VTGNVIPVDD149 pKa = 3.26IVLAPPRR156 pKa = 11.84DD157 pKa = 3.81PVTSGCEE164 pKa = 3.49ASDD167 pKa = 3.79FVGLDD172 pKa = 3.27FSGTADD178 pKa = 2.89IALIQRR184 pKa = 11.84GTCEE188 pKa = 4.2FGVKK192 pKa = 10.28AINAQTAGAEE202 pKa = 3.86AVIIFNQGNTEE213 pKa = 3.97LRR215 pKa = 11.84SGLVTGTLFGINQTPLSIPVVGASFADD242 pKa = 4.38GEE244 pKa = 4.3ALAAAGSTARR254 pKa = 11.84VRR256 pKa = 11.84VDD258 pKa = 3.53LPEE261 pKa = 4.4SRR263 pKa = 11.84PQVNVIAEE271 pKa = 4.18LAGRR275 pKa = 11.84TDD277 pKa = 3.65GNVVMAGAHH286 pKa = 6.37LDD288 pKa = 3.8SVQAGPGINDD298 pKa = 3.47NGSGSSALLEE308 pKa = 4.23VAQQIAKK315 pKa = 9.61VKK317 pKa = 10.31PEE319 pKa = 3.72NTLRR323 pKa = 11.84FAWWGAEE330 pKa = 3.73EE331 pKa = 4.42SGLLGSRR338 pKa = 11.84AYY340 pKa = 10.57VAGLDD345 pKa = 3.65QAGLDD350 pKa = 4.17EE351 pKa = 4.55IALYY355 pKa = 11.25LNFDD359 pKa = 3.93MVASPNYY366 pKa = 9.86IFMVYY371 pKa = 10.42DD372 pKa = 3.72GDD374 pKa = 3.85EE375 pKa = 4.42SGFPAPVVVPEE386 pKa = 4.46GSVQIEE392 pKa = 4.2DD393 pKa = 3.95FFEE396 pKa = 4.05SFYY399 pKa = 10.12TSRR402 pKa = 11.84GIPYY406 pKa = 10.22DD407 pKa = 3.51DD408 pKa = 4.89AEE410 pKa = 4.16FSGRR414 pKa = 11.84SDD416 pKa = 3.35YY417 pKa = 11.02QAFIQNGIPAGGLFTGAEE435 pKa = 4.39VVKK438 pKa = 10.77SPEE441 pKa = 3.66QAAIWGGTAGQQYY454 pKa = 10.41DD455 pKa = 3.78PCYY458 pKa = 10.51HH459 pKa = 7.05LACDD463 pKa = 4.06TIDD466 pKa = 4.29NISLEE471 pKa = 4.17ALDD474 pKa = 4.36VNTDD478 pKa = 4.12AIAAAVLTYY487 pKa = 10.23AYY489 pKa = 9.31STEE492 pKa = 4.21TVNGVVGQQVPGNFVLPAPAGPQGTTGSGGGDD524 pKa = 2.86EE525 pKa = 4.4VDD527 pKa = 3.5GVEE530 pKa = 4.16

Molecular weight: 54.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0T0MJC2|A0A0T0MJC2_9CELL Peptide-methionine (R)-S-oxide reductase OS=Cellulomonas sp. Leaf395 OX=1736362 GN=ASG23_08190 PE=4 SV=1
MM1 pKa = 7.56AVPKK5 pKa = 10.48RR6 pKa = 11.84KK7 pKa = 9.07MSRR10 pKa = 11.84SNTRR14 pKa = 11.84ARR16 pKa = 11.84RR17 pKa = 11.84SQWKK21 pKa = 6.29TTATTLHH28 pKa = 6.39TCPQCKK34 pKa = 9.28ANKK37 pKa = 8.6MPHH40 pKa = 5.35TACPSCGAYY49 pKa = 9.49KK50 pKa = 10.56GRR52 pKa = 11.84AYY54 pKa = 10.6AEE56 pKa = 3.93AVRR59 pKa = 11.84TEE61 pKa = 4.04FEE63 pKa = 4.11VRR65 pKa = 3.28

Molecular weight: 7.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3738

0

3738

1224298

37

2068

327.5

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.741 ± 0.057

0.555 ± 0.009

6.624 ± 0.035

5.265 ± 0.039

2.678 ± 0.026

9.077 ± 0.033

2.136 ± 0.022

3.369 ± 0.031

1.521 ± 0.023

10.441 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.595 ± 0.015

1.609 ± 0.025

5.794 ± 0.029

2.748 ± 0.022

7.526 ± 0.049

5.3 ± 0.03

6.489 ± 0.041

10.05 ± 0.042

1.604 ± 0.018

1.879 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski