Amino acid dipepetide frequency for Cellulomonas sp. Leaf395

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.903AlaAla: 20.903 ± 0.211	0.823AlaCys: 0.823 ± 0.029	9.168AlaAsp: 9.168 ± 0.103	7.434AlaGlu: 7.434 ± 0.093	3.624AlaPhe: 3.624 ± 0.06	12.503AlaGly: 12.503 ± 0.107	2.709AlaHis: 2.709 ± 0.049	4.582AlaIle: 4.582 ± 0.071	2.052AlaLys: 2.052 ± 0.044	14.708AlaLeu: 14.708 ± 0.144	2.542AlaMet: 2.542 ± 0.044	1.96AlaAsn: 1.96 ± 0.044	6.864AlaPro: 6.864 ± 0.092	4.102AlaGln: 4.102 ± 0.064	10.239AlaArg: 10.239 ± 0.133	6.872AlaSer: 6.872 ± 0.09	8.532AlaThr: 8.532 ± 0.1	13.156AlaVal: 13.156 ± 0.126	2.2AlaTrp: 2.2 ± 0.048	2.433AlaTyr: 2.433 ± 0.043	0.001AlaXaa: 0.001 ± 0.001
Cys	0.756CysAla: 0.756 ± 0.028	0.062CysCys: 0.062 ± 0.007	0.373CysAsp: 0.373 ± 0.021	0.268CysGlu: 0.268 ± 0.017	0.162CysPhe: 0.162 ± 0.012	0.598CysGly: 0.598 ± 0.027	0.145CysHis: 0.145 ± 0.011	0.155CysIle: 0.155 ± 0.011	0.056CysLys: 0.056 ± 0.006	0.483CysLeu: 0.483 ± 0.021	0.066CysMet: 0.066 ± 0.008	0.096CysAsn: 0.096 ± 0.009	0.347CysPro: 0.347 ± 0.017	0.127CysGln: 0.127 ± 0.01	0.377CysArg: 0.377 ± 0.019	0.344CysSer: 0.344 ± 0.019	0.418CysThr: 0.418 ± 0.016	0.537CysVal: 0.537 ± 0.021	0.08CysTrp: 0.08 ± 0.009	0.097CysTyr: 0.097 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	9.216AspAla: 9.216 ± 0.098	0.261AspCys: 0.261 ± 0.017	5.0AspAsp: 5.0 ± 0.077	4.579AspGlu: 4.579 ± 0.071	1.432AspPhe: 1.432 ± 0.034	7.226AspGly: 7.226 ± 0.097	1.303AspHis: 1.303 ± 0.038	1.751AspIle: 1.751 ± 0.043	0.901AspLys: 0.901 ± 0.027	7.231AspLeu: 7.231 ± 0.083	0.713AspMet: 0.713 ± 0.025	0.845AspAsn: 0.845 ± 0.028	4.791AspPro: 4.791 ± 0.064	1.737AspGln: 1.737 ± 0.036	4.8AspArg: 4.8 ± 0.063	2.448AspSer: 2.448 ± 0.044	2.975AspThr: 2.975 ± 0.048	7.011AspVal: 7.011 ± 0.08	0.967AspTrp: 0.967 ± 0.032	1.051AspTyr: 1.051 ± 0.031	0.0AspXaa: 0.0 ± 0.0
Glu	6.672GluAla: 6.672 ± 0.1	0.255GluCys: 0.255 ± 0.015	2.793GluAsp: 2.793 ± 0.05	2.492GluGlu: 2.492 ± 0.052	1.264GluPhe: 1.264 ± 0.034	3.682GluGly: 3.682 ± 0.056	1.65GluHis: 1.65 ± 0.041	2.308GluIle: 2.308 ± 0.043	1.012GluLys: 1.012 ± 0.032	6.525GluLeu: 6.525 ± 0.09	0.776GluMet: 0.776 ± 0.021	0.947GluAsn: 0.947 ± 0.026	3.292GluPro: 3.292 ± 0.057	2.156GluGln: 2.156 ± 0.044	4.839GluArg: 4.839 ± 0.075	2.558GluSer: 2.558 ± 0.054	2.593GluThr: 2.593 ± 0.044	5.116GluVal: 5.116 ± 0.062	0.698GluTrp: 0.698 ± 0.023	1.026GluTyr: 1.026 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	3.748PheAla: 3.748 ± 0.059	0.18PheCys: 0.18 ± 0.012	2.018PheAsp: 2.018 ± 0.042	1.407PheGlu: 1.407 ± 0.034	0.845PhePhe: 0.845 ± 0.035	2.898PheGly: 2.898 ± 0.057	0.551PheHis: 0.551 ± 0.023	0.709PheIle: 0.709 ± 0.026	0.36PheLys: 0.36 ± 0.017	2.553PheLeu: 2.553 ± 0.05	0.355PheMet: 0.355 ± 0.019	0.499PheAsn: 0.499 ± 0.022	1.264PhePro: 1.264 ± 0.034	0.674PheGln: 0.674 ± 0.025	1.648PheArg: 1.648 ± 0.037	1.471PheSer: 1.471 ± 0.045	1.846PheThr: 1.846 ± 0.042	2.7PheVal: 2.7 ± 0.053	0.476PheTrp: 0.476 ± 0.018	0.577PheTyr: 0.577 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	11.089GlyAla: 11.089 ± 0.1	0.585GlyCys: 0.585 ± 0.023	5.223GlyAsp: 5.223 ± 0.072	4.496GlyGlu: 4.496 ± 0.067	2.913GlyPhe: 2.913 ± 0.053	7.779GlyGly: 7.779 ± 0.094	2.026GlyHis: 2.026 ± 0.042	3.699GlyIle: 3.699 ± 0.05	1.839GlyLys: 1.839 ± 0.05	9.521GlyLeu: 9.521 ± 0.099	1.808GlyMet: 1.808 ± 0.042	1.489GlyAsn: 1.489 ± 0.043	4.702GlyPro: 4.702 ± 0.067	2.512GlyGln: 2.512 ± 0.049	6.9GlyArg: 6.9 ± 0.095	5.595GlySer: 5.595 ± 0.078	6.317GlyThr: 6.317 ± 0.081	8.279GlyVal: 8.279 ± 0.096	1.839GlyTrp: 1.839 ± 0.042	2.156GlyTyr: 2.156 ± 0.048	0.001GlyXaa: 0.001 ± 0.001
His	2.674HisAla: 2.674 ± 0.049	0.119HisCys: 0.119 ± 0.01	1.547HisAsp: 1.547 ± 0.041	1.208HisGlu: 1.208 ± 0.03	0.444HisPhe: 0.444 ± 0.02	2.203HisGly: 2.203 ± 0.045	0.599HisHis: 0.599 ± 0.022	0.543HisIle: 0.543 ± 0.02	0.289HisLys: 0.289 ± 0.017	2.462HisLeu: 2.462 ± 0.049	0.249HisMet: 0.249 ± 0.012	0.322HisAsn: 0.322 ± 0.018	1.572HisPro: 1.572 ± 0.037	0.555HisGln: 0.555 ± 0.019	1.751HisArg: 1.751 ± 0.039	0.823HisSer: 0.823 ± 0.026	1.099HisThr: 1.099 ± 0.029	2.184HisVal: 2.184 ± 0.039	0.313HisTrp: 0.313 ± 0.018	0.402HisTyr: 0.402 ± 0.019	0.001HisXaa: 0.001 ± 0.001
Ile	5.231IleAla: 5.231 ± 0.067	0.193IleCys: 0.193 ± 0.011	2.713IleAsp: 2.713 ± 0.043	2.162IleGlu: 2.162 ± 0.046	0.826IlePhe: 0.826 ± 0.03	3.585IleGly: 3.585 ± 0.058	0.564IleHis: 0.564 ± 0.023	1.086IleIle: 1.086 ± 0.041	0.576IleLys: 0.576 ± 0.025	2.676IleLeu: 2.676 ± 0.051	0.453IleMet: 0.453 ± 0.019	0.655IleAsn: 0.655 ± 0.026	1.867IlePro: 1.867 ± 0.037	0.716IleGln: 0.716 ± 0.023	2.032IleArg: 2.032 ± 0.041	1.71IleSer: 1.71 ± 0.04	2.232IleThr: 2.232 ± 0.043	3.453IleVal: 3.453 ± 0.055	0.381IleTrp: 0.381 ± 0.018	0.581IleTyr: 0.581 ± 0.02	0.001IleXaa: 0.001 ± 0.001
Lys	2.049LysAla: 2.049 ± 0.049	0.073LysCys: 0.073 ± 0.007	0.953LysAsp: 0.953 ± 0.031	0.748LysGlu: 0.748 ± 0.028	0.361LysPhe: 0.361 ± 0.016	1.219LysGly: 1.219 ± 0.037	0.384LysHis: 0.384 ± 0.017	0.674LysIle: 0.674 ± 0.025	0.464LysLys: 0.464 ± 0.026	1.407LysLeu: 1.407 ± 0.038	0.258LysMet: 0.258 ± 0.015	0.377LysAsn: 0.377 ± 0.018	0.951LysPro: 0.951 ± 0.029	0.435LysGln: 0.435 ± 0.019	1.071LysArg: 1.071 ± 0.033	0.807LysSer: 0.807 ± 0.028	0.933LysThr: 0.933 ± 0.03	1.513LysVal: 1.513 ± 0.039	0.167LysTrp: 0.167 ± 0.012	0.363LysTyr: 0.363 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	15.806LeuAla: 15.806 ± 0.15	0.578LeuCys: 0.578 ± 0.023	7.699LeuAsp: 7.699 ± 0.091	5.112LeuGlu: 5.112 ± 0.069	2.432LeuPhe: 2.432 ± 0.048	9.563LeuGly: 9.563 ± 0.092	2.098LeuHis: 2.098 ± 0.042	2.87LeuIle: 2.87 ± 0.059	1.247LeuLys: 1.247 ± 0.032	10.904LeuLeu: 10.904 ± 0.139	1.432LeuMet: 1.432 ± 0.038	1.488LeuAsn: 1.488 ± 0.039	5.981LeuPro: 5.981 ± 0.067	2.461LeuGln: 2.461 ± 0.045	8.051LeuArg: 8.051 ± 0.083	5.298LeuSer: 5.298 ± 0.07	6.855LeuThr: 6.855 ± 0.07	11.67LeuVal: 11.67 ± 0.116	1.309LeuTrp: 1.309 ± 0.035	1.546LeuTyr: 1.546 ± 0.041	0.003LeuXaa: 0.003 ± 0.001
Met	2.063MetAla: 2.063 ± 0.044	0.101MetCys: 0.101 ± 0.009	0.79MetAsp: 0.79 ± 0.027	0.533MetGlu: 0.533 ± 0.018	0.458MetPhe: 0.458 ± 0.02	1.09MetGly: 1.09 ± 0.031	0.354MetHis: 0.354 ± 0.016	0.662MetIle: 0.662 ± 0.022	0.27MetLys: 0.27 ± 0.016	1.616MetLeu: 1.616 ± 0.035	0.227MetMet: 0.227 ± 0.016	0.316MetAsn: 0.316 ± 0.014	0.992MetPro: 0.992 ± 0.031	0.421MetGln: 0.421 ± 0.018	1.265MetArg: 1.265 ± 0.031	1.32MetSer: 1.32 ± 0.036	1.699MetThr: 1.699 ± 0.034	1.322MetVal: 1.322 ± 0.035	0.194MetTrp: 0.194 ± 0.013	0.253MetTyr: 0.253 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	1.987AsnAla: 1.987 ± 0.046	0.096AsnCys: 0.096 ± 0.009	0.954AsnAsp: 0.954 ± 0.031	0.789AsnGlu: 0.789 ± 0.03	0.495AsnPhe: 0.495 ± 0.023	1.588AsnGly: 1.588 ± 0.047	0.372AsnHis: 0.372 ± 0.019	0.595AsnIle: 0.595 ± 0.024	0.283AsnLys: 0.283 ± 0.016	1.681AsnLeu: 1.681 ± 0.041	0.222AsnMet: 0.222 ± 0.015	0.367AsnAsn: 0.367 ± 0.022	1.357AsnPro: 1.357 ± 0.038	0.485AsnGln: 0.485 ± 0.021	1.048AsnArg: 1.048 ± 0.03	0.721AsnSer: 0.721 ± 0.024	0.924AsnThr: 0.924 ± 0.032	1.522AsnVal: 1.522 ± 0.038	0.222AsnTrp: 0.222 ± 0.014	0.386AsnTyr: 0.386 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	8.108ProAla: 8.108 ± 0.101	0.214ProCys: 0.214 ± 0.014	4.685ProAsp: 4.685 ± 0.067	3.449ProGlu: 3.449 ± 0.054	1.595ProPhe: 1.595 ± 0.035	5.762ProGly: 5.762 ± 0.072	1.162ProHis: 1.162 ± 0.029	1.543ProIle: 1.543 ± 0.036	0.77ProLys: 0.77 ± 0.025	4.994ProLeu: 4.994 ± 0.059	0.957ProMet: 0.957 ± 0.029	0.819ProAsn: 0.819 ± 0.029	3.069ProPro: 3.069 ± 0.069	1.57ProGln: 1.57 ± 0.041	3.607ProArg: 3.607 ± 0.053	3.451ProSer: 3.451 ± 0.053	4.284ProThr: 4.284 ± 0.083	5.884ProVal: 5.884 ± 0.074	0.955ProTrp: 0.955 ± 0.031	1.056ProTyr: 1.056 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	3.629GlnAla: 3.629 ± 0.051	0.124GlnCys: 0.124 ± 0.009	1.527GlnAsp: 1.527 ± 0.033	1.353GlnGlu: 1.353 ± 0.034	0.784GlnPhe: 0.784 ± 0.027	2.013GlnGly: 2.013 ± 0.036	0.657GlnHis: 0.657 ± 0.025	1.13GlnIle: 1.13 ± 0.028	0.454GlnLys: 0.454 ± 0.023	3.065GlnLeu: 3.065 ± 0.05	0.475GlnMet: 0.475 ± 0.019	0.471GlnAsn: 0.471 ± 0.021	1.581GlnPro: 1.581 ± 0.038	1.02GlnGln: 1.02 ± 0.035	2.309GlnArg: 2.309 ± 0.045	1.284GlnSer: 1.284 ± 0.036	1.508GlnThr: 1.508 ± 0.038	3.002GlnVal: 3.002 ± 0.056	0.505GlnTrp: 0.505 ± 0.019	0.591GlnTyr: 0.591 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	9.917ArgAla: 9.917 ± 0.117	0.372ArgCys: 0.372 ± 0.019	4.443ArgAsp: 4.443 ± 0.071	4.001ArgGlu: 4.001 ± 0.062	2.06ArgPhe: 2.06 ± 0.043	5.632ArgGly: 5.632 ± 0.08	1.741ArgHis: 1.741 ± 0.038	2.946ArgIle: 2.946 ± 0.044	1.095ArgLys: 1.095 ± 0.032	8.022ArgLeu: 8.022 ± 0.093	1.447ArgMet: 1.447 ± 0.032	1.068ArgAsn: 1.068 ± 0.03	4.267ArgPro: 4.267 ± 0.065	2.0ArgGln: 2.0 ± 0.039	7.147ArgArg: 7.147 ± 0.106	4.411ArgSer: 4.411 ± 0.058	5.165ArgThr: 5.165 ± 0.064	6.445ArgVal: 6.445 ± 0.079	1.497ArgTrp: 1.497 ± 0.037	1.58ArgTyr: 1.58 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	7.171SerAla: 7.171 ± 0.075	0.304SerCys: 0.304 ± 0.015	3.096SerAsp: 3.096 ± 0.054	2.375SerGlu: 2.375 ± 0.041	1.537SerPhe: 1.537 ± 0.035	5.569SerGly: 5.569 ± 0.073	0.965SerHis: 0.965 ± 0.031	1.857SerIle: 1.857 ± 0.037	0.745SerLys: 0.745 ± 0.026	4.967SerLeu: 4.967 ± 0.062	1.114SerMet: 1.114 ± 0.03	0.911SerAsn: 0.911 ± 0.031	3.133SerPro: 3.133 ± 0.052	1.286SerGln: 1.286 ± 0.037	3.65SerArg: 3.65 ± 0.057	3.273SerSer: 3.273 ± 0.058	4.025SerThr: 4.025 ± 0.068	4.937SerVal: 4.937 ± 0.072	0.986SerTrp: 0.986 ± 0.029	1.101SerTyr: 1.101 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	8.495ThrAla: 8.495 ± 0.106	0.371ThrCys: 0.371 ± 0.018	4.027ThrAsp: 4.027 ± 0.07	2.973ThrGlu: 2.973 ± 0.052	2.019ThrPhe: 2.019 ± 0.045	6.28ThrGly: 6.28 ± 0.086	1.26ThrHis: 1.26 ± 0.034	2.316ThrIle: 2.316 ± 0.051	0.972ThrLys: 0.972 ± 0.032	6.442ThrLeu: 6.442 ± 0.073	1.01ThrMet: 1.01 ± 0.032	1.118ThrAsn: 1.118 ± 0.034	4.291ThrPro: 4.291 ± 0.071	1.589ThrGln: 1.589 ± 0.036	3.956ThrArg: 3.956 ± 0.057	3.702ThrSer: 3.702 ± 0.068	4.772ThrThr: 4.772 ± 0.087	6.775ThrVal: 6.775 ± 0.108	1.197ThrTrp: 1.197 ± 0.035	1.322ThrTyr: 1.322 ± 0.039	0.001ThrXaa: 0.001 ± 0.001
Val	13.706ValAla: 13.706 ± 0.129	0.573ValCys: 0.573 ± 0.024	6.907ValAsp: 6.907 ± 0.081	5.58ValGlu: 5.58 ± 0.069	2.386ValPhe: 2.386 ± 0.049	8.576ValGly: 8.576 ± 0.096	2.124ValHis: 2.124 ± 0.044	3.274ValIle: 3.274 ± 0.05	1.255ValLys: 1.255 ± 0.037	11.237ValLeu: 11.237 ± 0.121	1.367ValMet: 1.367 ± 0.031	1.562ValAsn: 1.562 ± 0.038	5.84ValPro: 5.84 ± 0.076	2.509ValGln: 2.509 ± 0.04	7.608ValArg: 7.608 ± 0.093	4.853ValSer: 4.853 ± 0.064	6.426ValThr: 6.426 ± 0.093	11.83ValVal: 11.83 ± 0.129	1.335ValTrp: 1.335 ± 0.033	1.545ValTyr: 1.545 ± 0.04	0.001ValXaa: 0.001 ± 0.001
Trp	1.793TrpAla: 1.793 ± 0.04	0.138TrpCys: 0.138 ± 0.01	0.981TrpAsp: 0.981 ± 0.03	0.658TrpGlu: 0.658 ± 0.024	0.574TrpPhe: 0.574 ± 0.019	1.142TrpGly: 1.142 ± 0.033	0.392TrpHis: 0.392 ± 0.019	0.534TrpIle: 0.534 ± 0.02	0.269TrpLys: 0.269 ± 0.017	1.769TrpLeu: 1.769 ± 0.041	0.288TrpMet: 0.288 ± 0.014	0.408TrpAsn: 0.408 ± 0.02	0.794TrpPro: 0.794 ± 0.025	0.573TrpGln: 0.573 ± 0.023	1.329TrpArg: 1.329 ± 0.034	1.103TrpSer: 1.103 ± 0.03	1.161TrpThr: 1.161 ± 0.035	1.407TrpVal: 1.407 ± 0.036	0.415TrpTrp: 0.415 ± 0.019	0.313TrpTyr: 0.313 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.389TyrAla: 2.389 ± 0.048	0.126TyrCys: 0.126 ± 0.01	1.34TyrAsp: 1.34 ± 0.033	1.034TyrGlu: 1.034 ± 0.034	0.569TyrPhe: 0.569 ± 0.023	1.861TyrGly: 1.861 ± 0.04	0.302TyrHis: 0.302 ± 0.017	0.457TyrIle: 0.457 ± 0.019	0.297TyrLys: 0.297 ± 0.018	2.142TyrLeu: 2.142 ± 0.045	0.186TyrMet: 0.186 ± 0.013	0.375TyrAsn: 0.375 ± 0.017	0.979TyrPro: 0.979 ± 0.027	0.553TyrGln: 0.553 ± 0.021	1.628TyrArg: 1.628 ± 0.039	0.958TyrSer: 0.958 ± 0.034	1.122TyrThr: 1.122 ± 0.033	1.753TyrVal: 1.753 ± 0.043	0.305TyrTrp: 0.305 ± 0.016	0.413TyrTyr: 0.413 ± 0.021	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.005XaaAla: 0.005 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3738 proteins (1224298 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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