Streptococcus phage P7571

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Brussowvirus; unclassified Brussowvirus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286QPB9|A0A286QPB9_9CAUD Helicase OS=Streptococcus phage P7571 OX=1971427 GN=P7571_35 PE=4 SV=1
MM1 pKa = 7.12VNWVDD6 pKa = 4.58KK7 pKa = 11.1DD8 pKa = 4.24GNDD11 pKa = 4.31LPDD14 pKa = 4.6GEE16 pKa = 5.21SKK18 pKa = 10.84DD19 pKa = 4.08FKK21 pKa = 11.26AGMFFSFAGDD31 pKa = 3.56EE32 pKa = 4.15NNITDD37 pKa = 3.82TGEE40 pKa = 3.65GGYY43 pKa = 10.61YY44 pKa = 9.76GGYY47 pKa = 8.0YY48 pKa = 9.41YY49 pKa = 10.76RR50 pKa = 11.84RR51 pKa = 11.84FEE53 pKa = 4.06FGQFGTVWLSCWNKK67 pKa = 10.66DD68 pKa = 3.89DD69 pKa = 5.14LVNYY73 pKa = 7.38YY74 pKa = 8.26QQ75 pKa = 4.22

Molecular weight: 8.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A286QPD2|A0A286QPD2_9CAUD Endonuclease OS=Streptococcus phage P7571 OX=1971427 GN=P7571_27 PE=4 SV=1
MM1 pKa = 7.65LEE3 pKa = 3.9YY4 pKa = 10.54LASFRR9 pKa = 11.84LNSRR13 pKa = 11.84TVTSTFSRR21 pKa = 11.84GFLFFAKK28 pKa = 8.52KK29 pKa = 9.05TKK31 pKa = 10.27III33 pKa = 3.91

Molecular weight: 3.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

10717

33

1517

218.7

24.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.577 ± 1.244

0.644 ± 0.174

6.158 ± 0.452

6.606 ± 0.704

4.171 ± 0.193

6.746 ± 0.506

1.316 ± 0.203

7.017 ± 0.369

7.801 ± 0.646

7.754 ± 0.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.529 ± 0.316

5.655 ± 0.405

2.883 ± 0.23

3.686 ± 0.27

4.395 ± 0.42

6.858 ± 0.625

6.336 ± 0.32

6.802 ± 0.246

1.185 ± 0.182

3.882 ± 0.478
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski