Sphingomonas crocodyli

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4579 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A437LZU7|A0A437LZU7_9SPHN Cell division ATP-binding protein FtsE OS=Sphingomonas crocodyli OX=1979270 GN=ftsE PE=3 SV=1
MM1 pKa = 7.63AKK3 pKa = 10.15LVVITRR9 pKa = 11.84EE10 pKa = 3.93GDD12 pKa = 3.28EE13 pKa = 4.32SVYY16 pKa = 10.39EE17 pKa = 4.3AEE19 pKa = 5.3DD20 pKa = 3.74GLSVMEE26 pKa = 4.47VIRR29 pKa = 11.84DD30 pKa = 3.68NGVDD34 pKa = 3.99EE35 pKa = 5.6LLALCGGCCSCATCHH50 pKa = 6.68VIIDD54 pKa = 3.8PAFASLLPEE63 pKa = 4.12MSDD66 pKa = 5.33DD67 pKa = 3.99EE68 pKa = 5.54NEE70 pKa = 4.42LLDD73 pKa = 4.42SSDD76 pKa = 3.05HH77 pKa = 5.31RR78 pKa = 11.84TEE80 pKa = 4.2GSRR83 pKa = 11.84LSCQVIMKK91 pKa = 10.23DD92 pKa = 4.15DD93 pKa = 4.54LDD95 pKa = 4.18GMIVTIAPEE104 pKa = 3.85DD105 pKa = 3.4

Molecular weight: 11.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A437MBA0|A0A437MBA0_9SPHN Efflux RND transporter periplasmic adaptor subunit OS=Sphingomonas crocodyli OX=1979270 GN=EOD43_14220 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.61GFRR19 pKa = 11.84SRR21 pKa = 11.84SATPGGRR28 pKa = 11.84KK29 pKa = 9.04VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84NKK41 pKa = 10.45LSAA44 pKa = 3.94

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4579

0

4579

1452114

29

3681

317.1

34.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.258 ± 0.055

0.772 ± 0.01

6.163 ± 0.028

5.19 ± 0.035

3.577 ± 0.024

8.929 ± 0.048

1.992 ± 0.017

5.449 ± 0.024

3.159 ± 0.03

9.586 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.5 ± 0.018

2.569 ± 0.029

5.321 ± 0.029

2.986 ± 0.02

7.224 ± 0.043

5.201 ± 0.03

5.334 ± 0.038

7.097 ± 0.027

1.433 ± 0.016

2.259 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski