Solitalea koreensis
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2864 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A521DDR3|A0A521DDR3_9SPHI Uncharacterized protein OS=Solitalea koreensis OX=543615 GN=SAMN06265350_106220 PE=4 SV=1
MM1 pKa = 7.19 YY2 pKa = 7.77 WTLEE6 pKa = 4.21 LASHH10 pKa = 7.01 LEE12 pKa = 4.08 DD13 pKa = 4.93 APWPATKK20 pKa = 10.54 DD21 pKa = 3.44 EE22 pKa = 5.26 LIDD25 pKa = 3.48 YY26 pKa = 8.56 AIRR29 pKa = 11.84 SGAPVEE35 pKa = 4.53 VIEE38 pKa = 4.56 NLQALEE44 pKa = 4.69 DD45 pKa = 4.28 DD46 pKa = 4.31 GEE48 pKa = 4.31 PYY50 pKa = 11.04 EE51 pKa = 4.81 NIEE54 pKa = 4.55 EE55 pKa = 4.16 VWPDD59 pKa = 3.76 YY60 pKa = 7.77 PTKK63 pKa = 10.9 DD64 pKa = 2.91 DD65 pKa = 4.08 FLFNEE70 pKa = 4.77 DD71 pKa = 3.58 EE72 pKa = 4.45 YY73 pKa = 12.0
Molecular weight: 8.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.818
IPC2_protein 3.592
IPC_protein 3.528
Toseland 3.338
ProMoST 3.694
Dawson 3.516
Bjellqvist 3.681
Wikipedia 3.439
Rodwell 3.363
Grimsley 3.261
Solomon 3.478
Lehninger 3.439
Nozaki 3.643
DTASelect 3.795
Thurlkill 3.401
EMBOSS 3.452
Sillero 3.656
Patrickios 0.693
IPC_peptide 3.478
IPC2_peptide 3.617
IPC2.peptide.svr19 3.629
Protein with the highest isoelectric point:
>tr|A0A521DYT3|A0A521DYT3_9SPHI Uncharacterized protein OS=Solitalea koreensis OX=543615 GN=SAMN06265350_10978 PE=4 SV=1
MM1 pKa = 6.21 VRR3 pKa = 11.84 KK4 pKa = 9.98 RR5 pKa = 11.84 IKK7 pKa = 10.0 RR8 pKa = 11.84 GLRR11 pKa = 11.84 VSRR14 pKa = 11.84 YY15 pKa = 9.02 IIYY18 pKa = 10.44 KK19 pKa = 7.76 EE20 pKa = 3.88 TLVDD24 pKa = 3.64 YY25 pKa = 10.82 RR26 pKa = 11.84 EE27 pKa = 4.95 HH28 pKa = 5.62 IWSFIGAFIGIGIIAFIQSLSLAKK52 pKa = 10.52 LEE54 pKa = 4.0 NVFLIGSFGASSVLVYY70 pKa = 10.21 GAIQSPLAQPRR81 pKa = 11.84 NLIGGHH87 pKa = 5.36 FVSALVGVTVCHH99 pKa = 6.83 ILPDD103 pKa = 4.8 IIWLTASTAVAVSIVAMQITKK124 pKa = 9.64 TMHH127 pKa = 6.4 PPGGATALIAVTGSEE142 pKa = 4.33 KK143 pKa = 10.31 IKK145 pKa = 10.56 ALGYY149 pKa = 9.98 IYY151 pKa = 10.75 VLSPVLTGCLILLTVALIFNNMTSHH176 pKa = 7.29 RR177 pKa = 11.84 KK178 pKa = 9.24 YY179 pKa = 8.87 PTNKK183 pKa = 9.75 RR184 pKa = 11.84 FTNSLKK190 pKa = 10.6 RR191 pKa = 11.84 IITRR195 pKa = 11.84 RR196 pKa = 11.84 RR197 pKa = 11.84 TVRR200 pKa = 11.84 SPNSIKK206 pKa = 10.87
Molecular weight: 22.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 10.101
IPC_protein 10.906
Toseland 10.921
ProMoST 10.701
Dawson 11.023
Bjellqvist 10.745
Wikipedia 11.242
Rodwell 11.286
Grimsley 11.067
Solomon 11.155
Lehninger 11.111
Nozaki 10.906
DTASelect 10.745
Thurlkill 10.921
EMBOSS 11.33
Sillero 10.95
Patrickios 10.994
IPC_peptide 11.155
IPC2_peptide 9.78
IPC2.peptide.svr19 8.112
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2864
0
2864
989305
26
2903
345.4
38.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.149 ± 0.044
0.871 ± 0.015
5.179 ± 0.03
6.044 ± 0.045
5.04 ± 0.042
6.628 ± 0.04
1.824 ± 0.023
7.573 ± 0.05
7.503 ± 0.048
9.418 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.409 ± 0.022
5.777 ± 0.046
3.708 ± 0.027
3.559 ± 0.026
3.687 ± 0.03
6.514 ± 0.04
5.612 ± 0.058
6.427 ± 0.033
1.102 ± 0.017
3.977 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here