Bacillus aquiflavi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3304 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B3W129|A0A6B3W129_9BACI DUF2573 family protein OS=Bacillus aquiflavi OX=2672567 GN=G4D64_12625 PE=4 SV=1
MM1 pKa = 7.44LSSNKK6 pKa = 9.93LEE8 pKa = 3.96EE9 pKa = 4.17PVFQFLAGTFHH20 pKa = 7.22QDD22 pKa = 2.08IDD24 pKa = 4.21SPEE27 pKa = 3.9EE28 pKa = 3.74ALQEE32 pKa = 4.14LLTEE36 pKa = 4.14EE37 pKa = 4.07SKK39 pKa = 11.05EE40 pKa = 3.93YY41 pKa = 11.22LEE43 pKa = 4.07FAIIFLTDD51 pKa = 4.26FIGSEE56 pKa = 4.06YY57 pKa = 11.09SDD59 pKa = 3.32IEE61 pKa = 4.1KK62 pKa = 10.91NEE64 pKa = 4.19YY65 pKa = 9.41IQSCADD71 pKa = 3.13GVYY74 pKa = 10.01FPAIGLEE81 pKa = 3.96PLQWLYY87 pKa = 10.98QVIEE91 pKa = 4.33QIRR94 pKa = 11.84EE95 pKa = 3.91AVKK98 pKa = 10.58

Molecular weight: 11.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B3VUM5|A0A6B3VUM5_9BACI Coproporphyrinogen III oxidase OS=Bacillus aquiflavi OX=2672567 GN=G4D64_00510 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.6RR3 pKa = 11.84TFQPKK8 pKa = 8.56KK9 pKa = 8.74RR10 pKa = 11.84KK11 pKa = 9.01RR12 pKa = 11.84SKK14 pKa = 9.17IHH16 pKa = 5.66GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSKK25 pKa = 10.52NGRR28 pKa = 11.84KK29 pKa = 8.92ILASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.97ALSAA44 pKa = 4.07

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3304

0

3304

957062

14

2971

289.7

32.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.502 ± 0.048

0.732 ± 0.014

4.854 ± 0.033

7.659 ± 0.046

4.725 ± 0.045

6.519 ± 0.042

2.194 ± 0.021

8.453 ± 0.042

7.801 ± 0.038

9.837 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.655 ± 0.018

4.761 ± 0.033

3.442 ± 0.026

3.64 ± 0.03

3.993 ± 0.025

5.747 ± 0.028

5.296 ± 0.026

6.649 ± 0.037

0.95 ± 0.016

3.592 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski