Amino acid dipepetide frequency for Bacillus aquiflavi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.136AlaAla: 5.136 ± 0.102	0.568AlaCys: 0.568 ± 0.029	3.021AlaAsp: 3.021 ± 0.062	4.399AlaGlu: 4.399 ± 0.072	3.234AlaPhe: 3.234 ± 0.074	4.704AlaGly: 4.704 ± 0.091	1.313AlaHis: 1.313 ± 0.038	5.611AlaIle: 5.611 ± 0.083	4.912AlaLys: 4.912 ± 0.078	6.718AlaLeu: 6.718 ± 0.088	1.709AlaMet: 1.709 ± 0.046	2.997AlaAsn: 2.997 ± 0.063	1.834AlaPro: 1.834 ± 0.052	1.988AlaGln: 1.988 ± 0.049	2.421AlaArg: 2.421 ± 0.056	3.553AlaSer: 3.553 ± 0.06	3.151AlaThr: 3.151 ± 0.068	4.988AlaVal: 4.988 ± 0.084	0.529AlaTrp: 0.529 ± 0.027	2.236AlaTyr: 2.236 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	0.423CysAla: 0.423 ± 0.021	0.093CysCys: 0.093 ± 0.01	0.424CysAsp: 0.424 ± 0.022	0.486CysGlu: 0.486 ± 0.023	0.339CysPhe: 0.339 ± 0.02	0.611CysGly: 0.611 ± 0.026	0.202CysHis: 0.202 ± 0.013	0.574CysIle: 0.574 ± 0.025	0.442CysLys: 0.442 ± 0.023	0.682CysLeu: 0.682 ± 0.026	0.157CysMet: 0.157 ± 0.014	0.319CysAsn: 0.319 ± 0.019	0.356CysPro: 0.356 ± 0.024	0.264CysGln: 0.264 ± 0.018	0.301CysArg: 0.301 ± 0.02	0.518CysSer: 0.518 ± 0.026	0.402CysThr: 0.402 ± 0.018	0.381CysVal: 0.381 ± 0.019	0.075CysTrp: 0.075 ± 0.01	0.265CysTyr: 0.265 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	2.753AspAla: 2.753 ± 0.052	0.374AspCys: 0.374 ± 0.021	2.551AspAsp: 2.551 ± 0.059	4.411AspGlu: 4.411 ± 0.079	2.32AspPhe: 2.32 ± 0.052	3.176AspGly: 3.176 ± 0.072	1.41AspHis: 1.41 ± 0.041	4.074AspIle: 4.074 ± 0.067	3.232AspLys: 3.232 ± 0.064	4.884AspLeu: 4.884 ± 0.077	1.159AspMet: 1.159 ± 0.031	1.618AspAsn: 1.618 ± 0.05	1.831AspPro: 1.831 ± 0.045	1.906AspGln: 1.906 ± 0.045	2.245AspArg: 2.245 ± 0.052	2.382AspSer: 2.382 ± 0.052	2.081AspThr: 2.081 ± 0.048	3.489AspVal: 3.489 ± 0.061	0.551AspTrp: 0.551 ± 0.025	2.091AspTyr: 2.091 ± 0.052	0.0AspXaa: 0.0 ± 0.0
Glu	5.192GluAla: 5.192 ± 0.09	0.367GluCys: 0.367 ± 0.022	3.495GluAsp: 3.495 ± 0.065	7.044GluGlu: 7.044 ± 0.129	2.6GluPhe: 2.6 ± 0.048	3.991GluGly: 3.991 ± 0.067	1.496GluHis: 1.496 ± 0.041	6.315GluIle: 6.315 ± 0.092	7.896GluLys: 7.896 ± 0.108	7.432GluLeu: 7.432 ± 0.104	2.399GluMet: 2.399 ± 0.05	4.073GluAsn: 4.073 ± 0.067	1.872GluPro: 1.872 ± 0.055	3.578GluGln: 3.578 ± 0.078	3.693GluArg: 3.693 ± 0.077	3.073GluSer: 3.073 ± 0.053	4.012GluThr: 4.012 ± 0.075	4.918GluVal: 4.918 ± 0.084	0.74GluTrp: 0.74 ± 0.028	2.407GluTyr: 2.407 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	2.958PheAla: 2.958 ± 0.064	0.348PheCys: 0.348 ± 0.021	2.251PheAsp: 2.251 ± 0.061	3.011PheGlu: 3.011 ± 0.05	2.56PhePhe: 2.56 ± 0.074	3.206PheGly: 3.206 ± 0.067	1.151PheHis: 1.151 ± 0.036	4.536PheIle: 4.536 ± 0.09	2.836PheLys: 2.836 ± 0.057	4.881PheLeu: 4.881 ± 0.094	1.179PheMet: 1.179 ± 0.04	2.173PheAsn: 2.173 ± 0.049	1.71PhePro: 1.71 ± 0.043	1.601PheGln: 1.601 ± 0.046	1.55PheArg: 1.55 ± 0.044	3.352PheSer: 3.352 ± 0.065	2.614PheThr: 2.614 ± 0.05	3.049PheVal: 3.049 ± 0.054	0.452PheTrp: 0.452 ± 0.021	1.832PheTyr: 1.832 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	4.465GlyAla: 4.465 ± 0.081	0.597GlyCys: 0.597 ± 0.027	3.018GlyAsp: 3.018 ± 0.066	4.432GlyGlu: 4.432 ± 0.075	3.303GlyPhe: 3.303 ± 0.07	4.502GlyGly: 4.502 ± 0.1	1.382GlyHis: 1.382 ± 0.045	5.806GlyIle: 5.806 ± 0.083	5.447GlyLys: 5.447 ± 0.08	6.192GlyLeu: 6.192 ± 0.084	1.976GlyMet: 1.976 ± 0.055	2.697GlyAsn: 2.697 ± 0.054	1.589GlyPro: 1.589 ± 0.041	1.938GlyGln: 1.938 ± 0.049	2.528GlyArg: 2.528 ± 0.053	3.535GlySer: 3.535 ± 0.06	3.796GlyThr: 3.796 ± 0.062	4.636GlyVal: 4.636 ± 0.079	0.717GlyTrp: 0.717 ± 0.031	2.633GlyTyr: 2.633 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	1.207HisAla: 1.207 ± 0.038	0.228HisCys: 0.228 ± 0.015	1.098HisAsp: 1.098 ± 0.037	1.499HisGlu: 1.499 ± 0.037	1.272HisPhe: 1.272 ± 0.041	1.312HisGly: 1.312 ± 0.039	0.834HisHis: 0.834 ± 0.032	1.721HisIle: 1.721 ± 0.044	1.209HisLys: 1.209 ± 0.033	2.38HisLeu: 2.38 ± 0.056	0.481HisMet: 0.481 ± 0.022	0.82HisAsn: 0.82 ± 0.028	1.068HisPro: 1.068 ± 0.036	0.89HisGln: 0.89 ± 0.033	0.874HisArg: 0.874 ± 0.029	1.33HisSer: 1.33 ± 0.037	0.991HisThr: 0.991 ± 0.033	1.535HisVal: 1.535 ± 0.045	0.202HisTrp: 0.202 ± 0.015	0.994HisTyr: 0.994 ± 0.038	0.0HisXaa: 0.0 ± 0.0
Ile	5.906IleAla: 5.906 ± 0.1	0.665IleCys: 0.665 ± 0.03	4.842IleAsp: 4.842 ± 0.074	6.482IleGlu: 6.482 ± 0.099	3.861IlePhe: 3.861 ± 0.086	6.242IleGly: 6.242 ± 0.096	1.938IleHis: 1.938 ± 0.047	6.818IleIle: 6.818 ± 0.11	5.836IleLys: 5.836 ± 0.089	7.514IleLeu: 7.514 ± 0.117	1.788IleMet: 1.788 ± 0.041	4.03IleAsn: 4.03 ± 0.075	3.463IlePro: 3.463 ± 0.061	2.903IleGln: 2.903 ± 0.06	3.181IleArg: 3.181 ± 0.06	5.374IleSer: 5.374 ± 0.076	4.477IleThr: 4.477 ± 0.07	5.85IleVal: 5.85 ± 0.086	0.653IleTrp: 0.653 ± 0.027	2.709IleTyr: 2.709 ± 0.053	0.0IleXaa: 0.0 ± 0.0
Lys	5.026LysAla: 5.026 ± 0.074	0.395LysCys: 0.395 ± 0.023	4.032LysAsp: 4.032 ± 0.067	7.81LysGlu: 7.81 ± 0.11	2.261LysPhe: 2.261 ± 0.044	4.851LysGly: 4.851 ± 0.074	1.464LysHis: 1.464 ± 0.036	5.996LysIle: 5.996 ± 0.076	7.163LysLys: 7.163 ± 0.095	6.816LysLeu: 6.816 ± 0.087	2.463LysMet: 2.463 ± 0.054	4.039LysAsn: 4.039 ± 0.069	2.262LysPro: 2.262 ± 0.054	3.555LysGln: 3.555 ± 0.062	3.661LysArg: 3.661 ± 0.065	3.737LysSer: 3.737 ± 0.057	3.993LysThr: 3.993 ± 0.066	5.004LysVal: 5.004 ± 0.084	0.863LysTrp: 0.863 ± 0.033	2.615LysTyr: 2.615 ± 0.059	0.0LysXaa: 0.0 ± 0.0
Leu	6.778LeuAla: 6.778 ± 0.094	0.705LeuCys: 0.705 ± 0.032	4.344LeuAsp: 4.344 ± 0.066	6.747LeuGlu: 6.747 ± 0.094	5.271LeuPhe: 5.271 ± 0.102	5.9LeuGly: 5.9 ± 0.089	2.063LeuHis: 2.063 ± 0.045	7.894LeuIle: 7.894 ± 0.106	7.609LeuLys: 7.609 ± 0.103	10.271LeuLeu: 10.271 ± 0.143	2.356LeuMet: 2.356 ± 0.052	4.799LeuAsn: 4.799 ± 0.066	3.764LeuPro: 3.764 ± 0.064	3.811LeuGln: 3.811 ± 0.061	3.55LeuArg: 3.55 ± 0.07	6.669LeuSer: 6.669 ± 0.098	6.058LeuThr: 6.058 ± 0.08	5.828LeuVal: 5.828 ± 0.086	0.727LeuTrp: 0.727 ± 0.028	3.229LeuTyr: 3.229 ± 0.066	0.0LeuXaa: 0.0 ± 0.0
Met	1.667MetAla: 1.667 ± 0.043	0.124MetCys: 0.124 ± 0.011	1.247MetAsp: 1.247 ± 0.039	1.833MetGlu: 1.833 ± 0.043	1.096MetPhe: 1.096 ± 0.034	1.593MetGly: 1.593 ± 0.042	0.392MetHis: 0.392 ± 0.02	2.423MetIle: 2.423 ± 0.051	2.774MetLys: 2.774 ± 0.06	2.519MetLeu: 2.519 ± 0.055	0.902MetMet: 0.902 ± 0.027	1.644MetAsn: 1.644 ± 0.041	0.965MetPro: 0.965 ± 0.036	0.715MetGln: 0.715 ± 0.032	1.05MetArg: 1.05 ± 0.033	1.64MetSer: 1.64 ± 0.041	1.651MetThr: 1.651 ± 0.041	1.445MetVal: 1.445 ± 0.041	0.172MetTrp: 0.172 ± 0.016	0.697MetTyr: 0.697 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	2.695AsnAla: 2.695 ± 0.053	0.357AsnCys: 0.357 ± 0.018	2.51AsnAsp: 2.51 ± 0.056	4.421AsnGlu: 4.421 ± 0.071	1.679AsnPhe: 1.679 ± 0.04	3.508AsnGly: 3.508 ± 0.065	1.103AsnHis: 1.103 ± 0.039	4.242AsnIle: 4.242 ± 0.071	3.73AsnLys: 3.73 ± 0.063	3.902AsnLeu: 3.902 ± 0.068	1.262AsnMet: 1.262 ± 0.04	2.353AsnAsn: 2.353 ± 0.06	2.056AsnPro: 2.056 ± 0.052	1.807AsnGln: 1.807 ± 0.045	2.167AsnArg: 2.167 ± 0.05	2.368AsnSer: 2.368 ± 0.053	2.135AsnThr: 2.135 ± 0.048	3.13AsnVal: 3.13 ± 0.059	0.552AsnTrp: 0.552 ± 0.026	1.63AsnTyr: 1.63 ± 0.044	0.0AsnXaa: 0.0 ± 0.0
Pro	2.012ProAla: 2.012 ± 0.055	0.227ProCys: 0.227 ± 0.017	1.626ProAsp: 1.626 ± 0.043	2.682ProGlu: 2.682 ± 0.063	1.993ProPhe: 1.993 ± 0.057	2.107ProGly: 2.107 ± 0.061	0.811ProHis: 0.811 ± 0.032	3.112ProIle: 3.112 ± 0.056	2.386ProLys: 2.386 ± 0.047	3.499ProLeu: 3.499 ± 0.07	0.713ProMet: 0.713 ± 0.027	1.821ProAsn: 1.821 ± 0.05	1.017ProPro: 1.017 ± 0.037	1.105ProGln: 1.105 ± 0.039	1.044ProArg: 1.044 ± 0.04	2.062ProSer: 2.062 ± 0.043	1.839ProThr: 1.839 ± 0.054	2.592ProVal: 2.592 ± 0.051	0.368ProTrp: 0.368 ± 0.02	1.407ProTyr: 1.407 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	2.44GlnAla: 2.44 ± 0.053	0.209GlnCys: 0.209 ± 0.015	1.426GlnAsp: 1.426 ± 0.04	2.545GlnGlu: 2.545 ± 0.056	1.882GlnPhe: 1.882 ± 0.043	1.774GlnGly: 1.774 ± 0.046	0.794GlnHis: 0.794 ± 0.033	2.77GlnIle: 2.77 ± 0.055	2.979GlnLys: 2.979 ± 0.054	4.557GlnLeu: 4.557 ± 0.076	1.073GlnMet: 1.073 ± 0.028	1.648GlnAsn: 1.648 ± 0.048	1.075GlnPro: 1.075 ± 0.04	1.859GlnGln: 1.859 ± 0.055	1.444GlnArg: 1.444 ± 0.04	2.002GlnSer: 2.002 ± 0.045	1.959GlnThr: 1.959 ± 0.049	2.124GlnVal: 2.124 ± 0.049	0.374GlnTrp: 0.374 ± 0.022	1.463GlnTyr: 1.463 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	2.417ArgAla: 2.417 ± 0.054	0.275ArgCys: 0.275 ± 0.019	1.886ArgAsp: 1.886 ± 0.046	3.101ArgGlu: 3.101 ± 0.069	1.989ArgPhe: 1.989 ± 0.041	2.289ArgGly: 2.289 ± 0.054	0.861ArgHis: 0.861 ± 0.034	3.1ArgIle: 3.1 ± 0.058	3.641ArgLys: 3.641 ± 0.07	4.081ArgLeu: 4.081 ± 0.075	1.19ArgMet: 1.19 ± 0.03	1.985ArgAsn: 1.985 ± 0.044	1.261ArgPro: 1.261 ± 0.032	1.521ArgGln: 1.521 ± 0.041	1.749ArgArg: 1.749 ± 0.05	2.219ArgSer: 2.219 ± 0.054	2.048ArgThr: 2.048 ± 0.045	2.43ArgVal: 2.43 ± 0.057	0.395ArgTrp: 0.395 ± 0.02	1.485ArgTyr: 1.485 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	3.298SerAla: 3.298 ± 0.072	0.453SerCys: 0.453 ± 0.026	2.505SerAsp: 2.505 ± 0.051	3.893SerGlu: 3.893 ± 0.068	3.485SerPhe: 3.485 ± 0.063	4.168SerGly: 4.168 ± 0.078	1.21SerHis: 1.21 ± 0.036	5.214SerIle: 5.214 ± 0.077	4.06SerLys: 4.06 ± 0.062	6.081SerLeu: 6.081 ± 0.08	1.564SerMet: 1.564 ± 0.039	2.563SerAsn: 2.563 ± 0.055	2.068SerPro: 2.068 ± 0.042	1.761SerGln: 1.761 ± 0.04	2.156SerArg: 2.156 ± 0.047	3.67SerSer: 3.67 ± 0.08	2.938SerThr: 2.938 ± 0.059	3.629SerVal: 3.629 ± 0.066	0.585SerTrp: 0.585 ± 0.027	2.169SerTyr: 2.169 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	3.804ThrAla: 3.804 ± 0.071	0.344ThrCys: 0.344 ± 0.019	2.477ThrAsp: 2.477 ± 0.053	3.61ThrGlu: 3.61 ± 0.071	2.645ThrPhe: 2.645 ± 0.055	3.97ThrGly: 3.97 ± 0.069	0.996ThrHis: 0.996 ± 0.031	4.941ThrIle: 4.941 ± 0.073	3.699ThrLys: 3.699 ± 0.063	4.922ThrLeu: 4.922 ± 0.073	1.285ThrMet: 1.285 ± 0.036	2.594ThrAsn: 2.594 ± 0.055	2.164ThrPro: 2.164 ± 0.049	1.277ThrGln: 1.277 ± 0.04	1.77ThrArg: 1.77 ± 0.042	3.073ThrSer: 3.073 ± 0.062	2.818ThrThr: 2.818 ± 0.062	4.208ThrVal: 4.208 ± 0.068	0.433ThrTrp: 0.433 ± 0.02	1.93ThrTyr: 1.93 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	4.403ValAla: 4.403 ± 0.072	0.586ValCys: 0.586 ± 0.023	3.378ValAsp: 3.378 ± 0.055	4.648ValGlu: 4.648 ± 0.088	3.072ValPhe: 3.072 ± 0.06	4.198ValGly: 4.198 ± 0.072	1.356ValHis: 1.356 ± 0.037	5.908ValIle: 5.908 ± 0.079	4.962ValLys: 4.962 ± 0.08	6.294ValLeu: 6.294 ± 0.086	1.743ValMet: 1.743 ± 0.042	3.405ValAsn: 3.405 ± 0.056	2.431ValPro: 2.431 ± 0.049	2.27ValGln: 2.27 ± 0.046	2.521ValArg: 2.521 ± 0.051	4.239ValSer: 4.239 ± 0.057	3.758ValThr: 3.758 ± 0.067	4.477ValVal: 4.477 ± 0.087	0.578ValTrp: 0.578 ± 0.028	2.26ValTyr: 2.26 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.534TrpAla: 0.534 ± 0.025	0.078TrpCys: 0.078 ± 0.009	0.475TrpAsp: 0.475 ± 0.021	0.648TrpGlu: 0.648 ± 0.028	0.478TrpPhe: 0.478 ± 0.023	0.637TrpGly: 0.637 ± 0.029	0.212TrpHis: 0.212 ± 0.016	0.816TrpIle: 0.816 ± 0.033	0.772TrpLys: 0.772 ± 0.026	1.121TrpLeu: 1.121 ± 0.035	0.289TrpMet: 0.289 ± 0.018	0.513TrpAsn: 0.513 ± 0.027	0.228TrpPro: 0.228 ± 0.017	0.309TrpGln: 0.309 ± 0.02	0.38TrpArg: 0.38 ± 0.021	0.563TrpSer: 0.563 ± 0.025	0.446TrpThr: 0.446 ± 0.025	0.554TrpVal: 0.554 ± 0.026	0.118TrpTrp: 0.118 ± 0.012	0.331TrpTyr: 0.331 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.91TyrAla: 1.91 ± 0.049	0.322TyrCys: 0.322 ± 0.017	1.933TyrAsp: 1.933 ± 0.05	2.89TyrGlu: 2.89 ± 0.053	1.912TyrPhe: 1.912 ± 0.044	2.445TyrGly: 2.445 ± 0.057	0.956TyrHis: 0.956 ± 0.035	2.659TyrIle: 2.659 ± 0.056	2.423TyrLys: 2.423 ± 0.054	3.627TyrLeu: 3.627 ± 0.06	0.861TyrMet: 0.861 ± 0.03	1.514TyrAsn: 1.514 ± 0.043	1.408TyrPro: 1.408 ± 0.038	1.34TyrGln: 1.34 ± 0.035	1.641TyrArg: 1.641 ± 0.045	2.11TyrSer: 2.11 ± 0.049	1.791TyrThr: 1.791 ± 0.044	2.22TyrVal: 2.22 ± 0.051	0.421TyrTrp: 0.421 ± 0.021	1.536TyrTyr: 1.536 ± 0.044	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3304 proteins (957062 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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