Bombyx mori densovirus 3 (isolate Silkworm/China/Zhenjiang/-) (BmDNV-3)
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4U3C3|Q4U3C3_BMDND DNA-directed DNA polymerase OS=Bombyx mori densovirus 3 (isolate Silkworm/China/Zhenjiang/-) OX=648412 PE=4 SV=1
MM1 pKa = 7.16 NLKK4 pKa = 10.07 RR5 pKa = 11.84 LYY7 pKa = 10.04 VFCAVITAINSLSQSDD23 pKa = 4.85 LIQIRR28 pKa = 11.84 EE29 pKa = 4.3 LIEE32 pKa = 3.88 TNIIRR37 pKa = 11.84 LSKK40 pKa = 9.35 TFNGDD45 pKa = 2.16 ISTINNFYY53 pKa = 10.53 RR54 pKa = 11.84 ILVSVFEE61 pKa = 4.87 LFNDD65 pKa = 4.18 LLVEE69 pKa = 4.64 LDD71 pKa = 3.74 TPAVSNQLQLFEE83 pKa = 6.6 DD84 pKa = 4.77 EE85 pKa = 4.53 DD86 pKa = 4.66 TVPNDD91 pKa = 3.25 CLGIVGQMLIEE102 pKa = 4.17 YY103 pKa = 9.79 TDD105 pKa = 3.48 IPNDD109 pKa = 3.61 GSLTYY114 pKa = 10.96 LNIGTISSLTVTLDD128 pKa = 3.24 NQLYY132 pKa = 10.76 LILTVIYY139 pKa = 10.38 NNGRR143 pKa = 11.84 EE144 pKa = 4.37 TIQEE148 pKa = 3.96 VHH150 pKa = 5.45 NTGSPTIVVDD160 pKa = 4.62 FYY162 pKa = 11.21 EE163 pKa = 4.45 IKK165 pKa = 10.71 HH166 pKa = 6.04 NNTFQEE172 pKa = 4.26 IKK174 pKa = 9.79 TSIYY178 pKa = 9.53 YY179 pKa = 10.5 VNFINFVLEE188 pKa = 4.24 YY189 pKa = 8.77 TVHH192 pKa = 6.65 EE193 pKa = 4.36 IEE195 pKa = 5.95 DD196 pKa = 3.93 IIVTRR201 pKa = 11.84 NINRR205 pKa = 11.84 YY206 pKa = 7.18 NQWSTQQRR214 pKa = 11.84 SRR216 pKa = 11.84 SRR218 pKa = 11.84 IAFIQQEE225 pKa = 4.56 AIDD228 pKa = 3.83 PDD230 pKa = 3.43 IRR232 pKa = 11.84 NEE234 pKa = 3.84 QLQEE238 pKa = 3.91 SAILAKK244 pKa = 10.74 LHH246 pKa = 6.84 DD247 pKa = 4.44 AADD250 pKa = 3.57 TLEE253 pKa = 4.17 YY254 pKa = 10.95 YY255 pKa = 10.7 GISFLRR261 pKa = 11.84 EE262 pKa = 3.22 YY263 pKa = 10.67 YY264 pKa = 9.56 IAYY267 pKa = 8.94 FQYY270 pKa = 10.42 TIYY273 pKa = 11.21 AEE275 pKa = 4.28 TQKK278 pKa = 10.92 LDD280 pKa = 3.26 PEE282 pKa = 5.01 FYY284 pKa = 10.64 DD285 pKa = 5.86 DD286 pKa = 4.31 PLEE289 pKa = 4.62 KK290 pKa = 10.62 NVIYY294 pKa = 10.45 INRR297 pKa = 11.84 IVQALWRR304 pKa = 11.84 QQ305 pKa = 3.3
Molecular weight: 35.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.13
IPC2_protein 4.418
IPC_protein 4.368
Toseland 4.202
ProMoST 4.482
Dawson 4.317
Bjellqvist 4.469
Wikipedia 4.19
Rodwell 4.202
Grimsley 4.113
Solomon 4.317
Lehninger 4.266
Nozaki 4.431
DTASelect 4.596
Thurlkill 4.202
EMBOSS 4.215
Sillero 4.482
Patrickios 1.99
IPC_peptide 4.317
IPC2_peptide 4.469
IPC2.peptide.svr19 4.39
Protein with the highest isoelectric point:
>tr|Q4U3C2|Q4U3C2_BMDND Nonstructural protein OS=Bombyx mori densovirus 3 (isolate Silkworm/China/Zhenjiang/-) OX=648412 PE=4 SV=1
MM1 pKa = 6.99 EE2 pKa = 4.47 TVEE5 pKa = 4.1 LAPRR9 pKa = 11.84 YY10 pKa = 9.0 AQEE13 pKa = 4.41 AKK15 pKa = 10.5 PEE17 pKa = 4.05 GLLYY21 pKa = 10.23 PSLVQDD27 pKa = 4.18 LFVQFRR33 pKa = 11.84 PFIRR37 pKa = 11.84 GSIPSGAGQLNPISTTVQCITRR59 pKa = 11.84 GPTSAVGSLNCVQNPLQTVYY79 pKa = 10.29 TYY81 pKa = 10.41 PQDD84 pKa = 3.54 LRR86 pKa = 11.84 TIDD89 pKa = 3.68 VGDD92 pKa = 3.26 ITINLEE98 pKa = 3.71 PNQYY102 pKa = 10.23 IILRR106 pKa = 11.84 LRR108 pKa = 11.84 IISTGNCNFQWTVRR122 pKa = 11.84 QLNINFFCSSCSGPNLICLEE142 pKa = 3.97 QNNRR146 pKa = 11.84 YY147 pKa = 9.0 NNRR150 pKa = 11.84 GSALNDD156 pKa = 3.09 YY157 pKa = 10.54 SFDD160 pKa = 3.58 MNNIEE165 pKa = 5.16 VYY167 pKa = 10.17 DD168 pKa = 3.83 AYY170 pKa = 11.13 YY171 pKa = 10.46 VNEE174 pKa = 5.16 GISSAIYY181 pKa = 9.97 DD182 pKa = 4.38 LNPTGQLIINTRR194 pKa = 11.84 LRR196 pKa = 11.84 QRR198 pKa = 11.84 LFVLQTPSVAEE209 pKa = 3.81 YY210 pKa = 9.19 TINGITRR217 pKa = 11.84 PVIKK221 pKa = 10.46 FNNIQFNLGTIFLSNRR237 pKa = 11.84 DD238 pKa = 2.86 ICATFVQTTRR248 pKa = 11.84 LVIPRR253 pKa = 11.84 CDD255 pKa = 3.58 NAYY258 pKa = 10.47 DD259 pKa = 3.34 WNYY262 pKa = 10.59 LFNIPGNSLATGTGVKK278 pKa = 9.67 VCNNLPFRR286 pKa = 11.84 FGVQRR291 pKa = 11.84 CPWGPSALLPRR302 pKa = 11.84 YY303 pKa = 9.3 NFAWMNIIDD312 pKa = 4.22 QLQEE316 pKa = 4.04 LNMFNVTSFQYY327 pKa = 10.76 INFRR331 pKa = 11.84 YY332 pKa = 9.7 NYY334 pKa = 7.32 YY335 pKa = 9.01 TEE337 pKa = 6.25 RR338 pKa = 11.84 ILTNAPIASYY348 pKa = 11.24 NSAIDD353 pKa = 4.97 DD354 pKa = 4.25 LAQLIIDD361 pKa = 3.89 EE362 pKa = 4.55 GNRR365 pKa = 11.84 LDD367 pKa = 4.01 ALEE370 pKa = 4.63 DD371 pKa = 3.21 RR372 pKa = 11.84 VEE374 pKa = 4.42 RR375 pKa = 11.84 IEE377 pKa = 5.01 DD378 pKa = 3.8 YY379 pKa = 10.6 IIEE382 pKa = 4.19 SSKK385 pKa = 8.96 ITIWDD390 pKa = 3.61 VLNIATNLYY399 pKa = 10.03 DD400 pKa = 3.18 VAGIIRR406 pKa = 11.84 DD407 pKa = 3.62 SAKK410 pKa = 10.75 LIRR413 pKa = 11.84 TASNQACKK421 pKa = 10.44 SLRR424 pKa = 11.84 GFFKK428 pKa = 10.75 RR429 pKa = 11.84 NNVAFDD435 pKa = 3.42 IGNKK439 pKa = 8.96 IDD441 pKa = 3.29 SLTRR445 pKa = 11.84 VTRR448 pKa = 11.84 NNQFNPVEE456 pKa = 4.44 AINYY460 pKa = 8.5 GSIYY464 pKa = 9.83 DD465 pKa = 4.13 TIHH468 pKa = 6.57 GFRR471 pKa = 11.84 KK472 pKa = 8.5 TSISNPTIRR481 pKa = 11.84 NSNLDD486 pKa = 3.66 NIITPRR492 pKa = 11.84 VLHH495 pKa = 6.92 DD496 pKa = 4.04 NNFNLPSTSLDD507 pKa = 3.35 VKK509 pKa = 11.08 DD510 pKa = 5.72 LINTNNVISMTTQKK524 pKa = 11.19 SFMDD528 pKa = 3.86 RR529 pKa = 11.84 LRR531 pKa = 11.84 DD532 pKa = 3.69 PKK534 pKa = 10.64 TGTNGDD540 pKa = 3.21 IMPYY544 pKa = 10.49 GVVEE548 pKa = 4.26 TLASPIEE555 pKa = 4.05 QLGPLGKK562 pKa = 10.09 FGYY565 pKa = 10.23 NRR567 pKa = 11.84 DD568 pKa = 3.03 ISNPSLSNFNTNSKK582 pKa = 8.69 STYY585 pKa = 9.86 LFHH588 pKa = 6.91 TSVRR592 pKa = 11.84 TKK594 pKa = 10.83 SFEE597 pKa = 4.46 LDD599 pKa = 3.34 INNKK603 pKa = 7.77 WVFSTRR609 pKa = 11.84 YY610 pKa = 9.49 SGIGEE615 pKa = 4.16 PSVLGATTMPHH626 pKa = 5.59 QIKK629 pKa = 10.45 VGYY632 pKa = 10.19 LKK634 pKa = 10.16 IEE636 pKa = 4.23 YY637 pKa = 9.41 DD638 pKa = 3.85 LKK640 pKa = 11.11 AGTGNTVSLTRR651 pKa = 11.84 RR652 pKa = 11.84 NWNQVYY658 pKa = 10.54 KK659 pKa = 10.85 NDD661 pKa = 3.38 VEE663 pKa = 4.84 TYY665 pKa = 9.83 TEE667 pKa = 4.23 LDD669 pKa = 2.89 IDD671 pKa = 4.05 NLFKK675 pKa = 11.01 AFSSSKK681 pKa = 9.6 TYY683 pKa = 10.48 RR684 pKa = 11.84 EE685 pKa = 4.05 RR686 pKa = 11.84 QNEE689 pKa = 4.24 STDD692 pKa = 3.43 WKK694 pKa = 9.6 WLYY697 pKa = 10.46 ISFAAEE703 pKa = 3.33 QKK705 pKa = 8.9 YY706 pKa = 8.2 NQRR709 pKa = 11.84 KK710 pKa = 9.14 VIQTQPIANPLYY722 pKa = 10.51 LKK724 pKa = 10.9 FMDD727 pKa = 4.54 RR728 pKa = 11.84 LFDD731 pKa = 3.77 INFRR735 pKa = 11.84 DD736 pKa = 3.86 GRR738 pKa = 11.84 LSGFKK743 pKa = 10.89 YY744 pKa = 10.67 NLLNQNCQTFAKK756 pKa = 8.99 TQSQLLTRR764 pKa = 11.84 GITNVQINDD773 pKa = 3.28 QGLLGFLEE781 pKa = 4.48 NYY783 pKa = 9.11 KK784 pKa = 10.55 QIARR788 pKa = 11.84 RR789 pKa = 11.84 EE790 pKa = 4.03 FSLSSGMTEE799 pKa = 4.12 TSWGSTSFVVRR810 pKa = 11.84 ALEE813 pKa = 3.91 YY814 pKa = 10.46 SKK816 pKa = 11.79 SNICYY821 pKa = 8.74 YY822 pKa = 11.31 AKK824 pKa = 10.23 INNMSSNIKK833 pKa = 9.54 IKK835 pKa = 10.43 KK836 pKa = 8.49 NRR838 pKa = 11.84 YY839 pKa = 8.9 SILDD843 pKa = 3.47 KK844 pKa = 10.8 AYY846 pKa = 10.02 KK847 pKa = 9.86 NDD849 pKa = 3.18 NTIRR853 pKa = 11.84 TVICTT858 pKa = 3.58
Molecular weight: 97.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.079
IPC2_protein 8.214
IPC_protein 8.053
Toseland 7.951
ProMoST 8.551
Dawson 8.785
Bjellqvist 8.946
Wikipedia 8.843
Rodwell 8.829
Grimsley 7.995
Solomon 8.843
Lehninger 8.814
Nozaki 8.99
DTASelect 8.756
Thurlkill 8.785
EMBOSS 8.916
Sillero 9.033
Patrickios 4.088
IPC_peptide 8.829
IPC2_peptide 7.834
IPC2.peptide.svr19 8.012
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
3441
126
1115
491.6
56.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.359 ± 0.486
1.715 ± 0.309
6.132 ± 0.609
5.551 ± 0.595
5.086 ± 0.387
5.173 ± 0.819
1.453 ± 0.384
8.66 ± 0.507
6.452 ± 1.132
8.37 ± 0.529
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.598 ± 0.186
8.166 ± 0.714
4.098 ± 0.236
3.226 ± 0.64
4.621 ± 0.723
7.469 ± 0.784
6.423 ± 0.448
4.911 ± 0.308
1.104 ± 0.247
5.434 ± 0.478
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here