Bombyx mori densovirus 3 (isolate Silkworm/China/Zhenjiang/-) (BmDNV-3)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Mouviricetes; Polivirales; Bidnaviridae; Bidensovirus; Bombyx mori bidensovirus; Bombyx mori densovirus 3

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q4U3C3|Q4U3C3_BMDND DNA-directed DNA polymerase OS=Bombyx mori densovirus 3 (isolate Silkworm/China/Zhenjiang/-) OX=648412 PE=4 SV=1
MM1 pKa = 7.16NLKK4 pKa = 10.07RR5 pKa = 11.84LYY7 pKa = 10.04VFCAVITAINSLSQSDD23 pKa = 4.85LIQIRR28 pKa = 11.84EE29 pKa = 4.3LIEE32 pKa = 3.88TNIIRR37 pKa = 11.84LSKK40 pKa = 9.35TFNGDD45 pKa = 2.16ISTINNFYY53 pKa = 10.53RR54 pKa = 11.84ILVSVFEE61 pKa = 4.87LFNDD65 pKa = 4.18LLVEE69 pKa = 4.64LDD71 pKa = 3.74TPAVSNQLQLFEE83 pKa = 6.6DD84 pKa = 4.77EE85 pKa = 4.53DD86 pKa = 4.66TVPNDD91 pKa = 3.25CLGIVGQMLIEE102 pKa = 4.17YY103 pKa = 9.79TDD105 pKa = 3.48IPNDD109 pKa = 3.61GSLTYY114 pKa = 10.96LNIGTISSLTVTLDD128 pKa = 3.24NQLYY132 pKa = 10.76LILTVIYY139 pKa = 10.38NNGRR143 pKa = 11.84EE144 pKa = 4.37TIQEE148 pKa = 3.96VHH150 pKa = 5.45NTGSPTIVVDD160 pKa = 4.62FYY162 pKa = 11.21EE163 pKa = 4.45IKK165 pKa = 10.71HH166 pKa = 6.04NNTFQEE172 pKa = 4.26IKK174 pKa = 9.79TSIYY178 pKa = 9.53YY179 pKa = 10.5VNFINFVLEE188 pKa = 4.24YY189 pKa = 8.77TVHH192 pKa = 6.65EE193 pKa = 4.36IEE195 pKa = 5.95DD196 pKa = 3.93IIVTRR201 pKa = 11.84NINRR205 pKa = 11.84YY206 pKa = 7.18NQWSTQQRR214 pKa = 11.84SRR216 pKa = 11.84SRR218 pKa = 11.84IAFIQQEE225 pKa = 4.56AIDD228 pKa = 3.83PDD230 pKa = 3.43IRR232 pKa = 11.84NEE234 pKa = 3.84QLQEE238 pKa = 3.91SAILAKK244 pKa = 10.74LHH246 pKa = 6.84DD247 pKa = 4.44AADD250 pKa = 3.57TLEE253 pKa = 4.17YY254 pKa = 10.95YY255 pKa = 10.7GISFLRR261 pKa = 11.84EE262 pKa = 3.22YY263 pKa = 10.67YY264 pKa = 9.56IAYY267 pKa = 8.94FQYY270 pKa = 10.42TIYY273 pKa = 11.21AEE275 pKa = 4.28TQKK278 pKa = 10.92LDD280 pKa = 3.26PEE282 pKa = 5.01FYY284 pKa = 10.64DD285 pKa = 5.86DD286 pKa = 4.31PLEE289 pKa = 4.62KK290 pKa = 10.62NVIYY294 pKa = 10.45INRR297 pKa = 11.84IVQALWRR304 pKa = 11.84QQ305 pKa = 3.3

Molecular weight:
35.62 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q4U3C2|Q4U3C2_BMDND Nonstructural protein OS=Bombyx mori densovirus 3 (isolate Silkworm/China/Zhenjiang/-) OX=648412 PE=4 SV=1
MM1 pKa = 6.99EE2 pKa = 4.47TVEE5 pKa = 4.1LAPRR9 pKa = 11.84YY10 pKa = 9.0AQEE13 pKa = 4.41AKK15 pKa = 10.5PEE17 pKa = 4.05GLLYY21 pKa = 10.23PSLVQDD27 pKa = 4.18LFVQFRR33 pKa = 11.84PFIRR37 pKa = 11.84GSIPSGAGQLNPISTTVQCITRR59 pKa = 11.84GPTSAVGSLNCVQNPLQTVYY79 pKa = 10.29TYY81 pKa = 10.41PQDD84 pKa = 3.54LRR86 pKa = 11.84TIDD89 pKa = 3.68VGDD92 pKa = 3.26ITINLEE98 pKa = 3.71PNQYY102 pKa = 10.23IILRR106 pKa = 11.84LRR108 pKa = 11.84IISTGNCNFQWTVRR122 pKa = 11.84QLNINFFCSSCSGPNLICLEE142 pKa = 3.97QNNRR146 pKa = 11.84YY147 pKa = 9.0NNRR150 pKa = 11.84GSALNDD156 pKa = 3.09YY157 pKa = 10.54SFDD160 pKa = 3.58MNNIEE165 pKa = 5.16VYY167 pKa = 10.17DD168 pKa = 3.83AYY170 pKa = 11.13YY171 pKa = 10.46VNEE174 pKa = 5.16GISSAIYY181 pKa = 9.97DD182 pKa = 4.38LNPTGQLIINTRR194 pKa = 11.84LRR196 pKa = 11.84QRR198 pKa = 11.84LFVLQTPSVAEE209 pKa = 3.81YY210 pKa = 9.19TINGITRR217 pKa = 11.84PVIKK221 pKa = 10.46FNNIQFNLGTIFLSNRR237 pKa = 11.84DD238 pKa = 2.86ICATFVQTTRR248 pKa = 11.84LVIPRR253 pKa = 11.84CDD255 pKa = 3.58NAYY258 pKa = 10.47DD259 pKa = 3.34WNYY262 pKa = 10.59LFNIPGNSLATGTGVKK278 pKa = 9.67VCNNLPFRR286 pKa = 11.84FGVQRR291 pKa = 11.84CPWGPSALLPRR302 pKa = 11.84YY303 pKa = 9.3NFAWMNIIDD312 pKa = 4.22QLQEE316 pKa = 4.04LNMFNVTSFQYY327 pKa = 10.76INFRR331 pKa = 11.84YY332 pKa = 9.7NYY334 pKa = 7.32YY335 pKa = 9.01TEE337 pKa = 6.25RR338 pKa = 11.84ILTNAPIASYY348 pKa = 11.24NSAIDD353 pKa = 4.97DD354 pKa = 4.25LAQLIIDD361 pKa = 3.89EE362 pKa = 4.55GNRR365 pKa = 11.84LDD367 pKa = 4.01ALEE370 pKa = 4.63DD371 pKa = 3.21RR372 pKa = 11.84VEE374 pKa = 4.42RR375 pKa = 11.84IEE377 pKa = 5.01DD378 pKa = 3.8YY379 pKa = 10.6IIEE382 pKa = 4.19SSKK385 pKa = 8.96ITIWDD390 pKa = 3.61VLNIATNLYY399 pKa = 10.03DD400 pKa = 3.18VAGIIRR406 pKa = 11.84DD407 pKa = 3.62SAKK410 pKa = 10.75LIRR413 pKa = 11.84TASNQACKK421 pKa = 10.44SLRR424 pKa = 11.84GFFKK428 pKa = 10.75RR429 pKa = 11.84NNVAFDD435 pKa = 3.42IGNKK439 pKa = 8.96IDD441 pKa = 3.29SLTRR445 pKa = 11.84VTRR448 pKa = 11.84NNQFNPVEE456 pKa = 4.44AINYY460 pKa = 8.5GSIYY464 pKa = 9.83DD465 pKa = 4.13TIHH468 pKa = 6.57GFRR471 pKa = 11.84KK472 pKa = 8.5TSISNPTIRR481 pKa = 11.84NSNLDD486 pKa = 3.66NIITPRR492 pKa = 11.84VLHH495 pKa = 6.92DD496 pKa = 4.04NNFNLPSTSLDD507 pKa = 3.35VKK509 pKa = 11.08DD510 pKa = 5.72LINTNNVISMTTQKK524 pKa = 11.19SFMDD528 pKa = 3.86RR529 pKa = 11.84LRR531 pKa = 11.84DD532 pKa = 3.69PKK534 pKa = 10.64TGTNGDD540 pKa = 3.21IMPYY544 pKa = 10.49GVVEE548 pKa = 4.26TLASPIEE555 pKa = 4.05QLGPLGKK562 pKa = 10.09FGYY565 pKa = 10.23NRR567 pKa = 11.84DD568 pKa = 3.03ISNPSLSNFNTNSKK582 pKa = 8.69STYY585 pKa = 9.86LFHH588 pKa = 6.91TSVRR592 pKa = 11.84TKK594 pKa = 10.83SFEE597 pKa = 4.46LDD599 pKa = 3.34INNKK603 pKa = 7.77WVFSTRR609 pKa = 11.84YY610 pKa = 9.49SGIGEE615 pKa = 4.16PSVLGATTMPHH626 pKa = 5.59QIKK629 pKa = 10.45VGYY632 pKa = 10.19LKK634 pKa = 10.16IEE636 pKa = 4.23YY637 pKa = 9.41DD638 pKa = 3.85LKK640 pKa = 11.11AGTGNTVSLTRR651 pKa = 11.84RR652 pKa = 11.84NWNQVYY658 pKa = 10.54KK659 pKa = 10.85NDD661 pKa = 3.38VEE663 pKa = 4.84TYY665 pKa = 9.83TEE667 pKa = 4.23LDD669 pKa = 2.89IDD671 pKa = 4.05NLFKK675 pKa = 11.01AFSSSKK681 pKa = 9.6TYY683 pKa = 10.48RR684 pKa = 11.84EE685 pKa = 4.05RR686 pKa = 11.84QNEE689 pKa = 4.24STDD692 pKa = 3.43WKK694 pKa = 9.6WLYY697 pKa = 10.46ISFAAEE703 pKa = 3.33QKK705 pKa = 8.9YY706 pKa = 8.2NQRR709 pKa = 11.84KK710 pKa = 9.14VIQTQPIANPLYY722 pKa = 10.51LKK724 pKa = 10.9FMDD727 pKa = 4.54RR728 pKa = 11.84LFDD731 pKa = 3.77INFRR735 pKa = 11.84DD736 pKa = 3.86GRR738 pKa = 11.84LSGFKK743 pKa = 10.89YY744 pKa = 10.67NLLNQNCQTFAKK756 pKa = 8.99TQSQLLTRR764 pKa = 11.84GITNVQINDD773 pKa = 3.28QGLLGFLEE781 pKa = 4.48NYY783 pKa = 9.11KK784 pKa = 10.55QIARR788 pKa = 11.84RR789 pKa = 11.84EE790 pKa = 4.03FSLSSGMTEE799 pKa = 4.12TSWGSTSFVVRR810 pKa = 11.84ALEE813 pKa = 3.91YY814 pKa = 10.46SKK816 pKa = 11.79SNICYY821 pKa = 8.74YY822 pKa = 11.31AKK824 pKa = 10.23INNMSSNIKK833 pKa = 9.54IKK835 pKa = 10.43KK836 pKa = 8.49NRR838 pKa = 11.84YY839 pKa = 8.9SILDD843 pKa = 3.47KK844 pKa = 10.8AYY846 pKa = 10.02KK847 pKa = 9.86NDD849 pKa = 3.18NTIRR853 pKa = 11.84TVICTT858 pKa = 3.58

Molecular weight:
97.95 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3441

126

1115

491.6

56.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.359 ± 0.486

1.715 ± 0.309

6.132 ± 0.609

5.551 ± 0.595

5.086 ± 0.387

5.173 ± 0.819

1.453 ± 0.384

8.66 ± 0.507

6.452 ± 1.132

8.37 ± 0.529

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.598 ± 0.186

8.166 ± 0.714

4.098 ± 0.236

3.226 ± 0.64

4.621 ± 0.723

7.469 ± 0.784

6.423 ± 0.448

4.911 ± 0.308

1.104 ± 0.247

5.434 ± 0.478

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski